JP2001500006A - 線虫及び/又はアリマキに対する耐性 - Google Patents
線虫及び/又はアリマキに対する耐性Info
- Publication number
- JP2001500006A JP2001500006A JP10509387A JP50938798A JP2001500006A JP 2001500006 A JP2001500006 A JP 2001500006A JP 10509387 A JP10509387 A JP 10509387A JP 50938798 A JP50938798 A JP 50938798A JP 2001500006 A JP2001500006 A JP 2001500006A
- Authority
- JP
- Japan
- Prior art keywords
- plant
- dna
- sequence
- nucleic acid
- dna sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- C07—ORGANIC CHEMISTRY
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- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
Description
Claims (1)
- 【特許請求の範囲】 1.DNA配列が、図5のDNA若しくはその一部、又は図5のDNA配列に相同なDNA 配列である核酸。 2.植物病原体に感受性がある宿主植物に移入されたときに、該宿主植物に前 記植物病原体に対する耐性を与え得る請求項1の核酸。 3.DNA配列が、図5に示されているDNA配列の少なくとも一部である核酸に相 当するcDNAである核酸配列。 4.宿主植物に移入されたときに、線虫に対する耐性を与えることができる請 求項1又は3の核酸。 5.宿主植物に移入されたときに、アリマキに対する耐性を与えることができ る請求項1又は3の核酸。 6.宿主植物に移入されたときに、線虫及びアリマキに対する耐性を与えるこ とができる請求項1又は3の核酸。 7.前記DNA配列が、図5の配列のヌクレオチド3263から始まり、ヌクレオチ ド7111に終わる配列又はこれと相同な任意のDNA配列に相当する核酸。 8.前記DNA配列が、ヌクレオチド3263の5'上流に位置するプロモーター配列 又はこれと相同な任意のDNA配列に相当する核酸。 9.前記DNA配列が、コスミドMi-11中に存在するゲノムインサートの少なくと も一部又はこれと相同な任意のDNA配列に相当する請求項1又は3の核酸。 10.請求項1〜9の何れか1項に記載の核酸を具備する組換えDNA構築物。 11.前記核酸が、植物細胞中で機能するプロモーターの調節下にあり、該プ ロモーターが前記植物細胞に内在しているか、又は外在しており、このような植 物細胞中の前記DNA配列の転写を調節するのに有効である請求項10の組換えDNA 構築物。 12 前記プロモーターが、図5に示されているヌクレオチド3263の5'上流に 位置するプロモーター配列又はこれに相同な任意のDNA配列に相当する請求項1 1の組換えDNA構築物。 13 請求項10〜12の何れが1項に記載のDNA構築物を具備する植物細胞を 形質転換するのに適したベクター。 14 番号CBS822.96で寄託したプラスミドpKGMi-11。 15.番号CBS821.96で寄託したプラスミドpKGMi-18。 16.請求項13〜15の何れか1項に記載のベクター又はプラスミドを具備 する細菌細胞。 17.請求項10〜12の何れか1項に記載のDNA構築物が取り込まれ、該DNA 構築物を具備する組換え植物ゲノム。 18 請求項10〜12の何れか1項に記載のDNA構築物を具備する植物細胞 。 19.請求項18に記載の植物細胞を具備する植物。 20.線虫に対しで減少した感受性を有する請求項19に記載の植物。 21.前記線虫が、根こぶ線虫、特にサツマイモネコブセンチュウ(Meloidogy ne incognita)である植物。 22.アリマキ、特にマクロサイファム・ユーフォオービア(Macrosiphum eu phorblae)に対して減少した感受性を有する請求項19に記載の植物。 23.請求項10〜12の何れか1項に記載のDNA構築物を具備する種子。 24.植物細胞環境中に存在する請求項17の組換え植物ゲノム。 25.病原体に対して減少した感受性を有する植物を得る方法であって、以下 のステップ: (1)請求項10〜12の何れか1項に記載のDNA構築物を植物細胞のゲノムに挿入す ることと、 (2)被形質転換細胞を得ることと、 (3)前記被形質転換細胞から遺伝的に形質転換された植物を再生すること、及 び (4)必要に応じて、前記植物を繁殖させること、 とを具備する方法。 26.請求項25に記載の方法であって、前記病原体が線虫、好ましくは、根 こぶ線虫、特にサツマイモネコブセンチュウである方法。 27.前記病原体がアリマキ、好ましくはマクロサイファム・ユーフォービア である請求項25に記載の方法。 28.栽培している植物を病原体の感染から守るための方法であって、 (1)請求項10〜12の何れか1項に記載のDNA構築物を植物のゲノムに供給す ることと、 (2)前記植物を増殖させること とを具備する方法。 29.請求項1〜6の何れか1項に記載の核酸を単離する方法であって、以下 のステップ: (1)請求項1〜9の何れか1項に記載のDNA配列を用いて、植物のゲノムライブ ラリー又はcDNAライブラリーをスクリーニングすることと、 (2)前記DNA配列にハイブリダイズするポジティブクローンを同定することと、 (3)前記ポジティブクローンを単離すること とを具備する方法。 30 前記ライブリーが第一の植物に由来し、前記DNA配列が第二の植物に属 する請求項29の方法。 31.表3に図示されているAFLPマーカーPM02〜PM29のうちの少なくとも一つ を同定するプライマーの組み合わせを用いて、請求項1〜9に記載の核酸を同定 するための選択的制限断片増幅法。 32.前記プライマーの組み合わせがAFLPマーカーPM14を同定する請求項31 の方法。 33 請求項1〜9に記載の核酸の少なくとも一部に相当するDNA配列を具備 するオリゴヌクレオチド。 34.ストリンジェントなハイブリダイゼーション条件下で、請求項1〜9の 何れか1項に記載のDNA配列に選択的にハイブリダイズするのに十分なサイズで ある請求項33のオリゴヌクレオチド。 35 前記DNA配列が、ヌクレオチド6921から始まり、ヌクレオチド7034で終 わる配列に相当ずる請求項34に記載のオリゴヌクレオチド。 36.前記DNA配列が3'末端に存在し、好ましくは、DNAの合成を開始させ得る 配列5'TGCAGGA-3'に相当する請求項35に記載のオリゴヌクレオチド。 37.前記DNA配列が3'末端に存在し、好ましくは、DNAの合成を開始させ得る 配列5'TAATCT-3'に相当する請求項35に記載のオリゴヌクレオチド。 38.請求項36に記載の第一のオリゴヌクレオチド及び請求項37に記載の 第二のヌクレオチドを具備するプライマーの組み合わせ。 39.請求項33〜37の何れか1項に記載のオリゴヌクレオチドのうちの少 なくとも1つを具備する診断用キット。 40.請求項38に記載のプライマーの組み合わせを具備する診断用キット。 41.請求項39又は40に記載の診断用キットを用いて、特に植物DNA中に おける請求項1〜9に記載のDNA配列の存在又は不存在を検出するための方法。 42.請求項1〜13の何れか1項に記載の組換えDNAの核酸の発現産物であ るポリペプチド。 43.図7Aに示されている配列を有するアミノ酸配列、又は請求項1〜3の何 れか1項に記載されている対応する相同性配列にコードされるアミノ酸配列を有 するポリペプチド。 44.請求項1〜3の何れか1項のDNA配列の一部又は全部の転写物のリボ核 酸配列を有するRNA。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
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EP96401764A EP0823481A1 (en) | 1996-08-09 | 1996-08-09 | Resistance against nematodes |
EP96401764.4 | 1996-08-09 | ||
EP97401101.7 | 1997-05-16 | ||
EP97401101 | 1997-05-16 | ||
PCT/EP1997/004340 WO1998006750A2 (en) | 1996-08-09 | 1997-08-08 | Resistance against nematodes and/or aphids |
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JP2008126165A Division JP2008289481A (ja) | 1996-08-09 | 2008-05-13 | 線虫及び/又はアリマキに対する耐性 |
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JP2001500006A true JP2001500006A (ja) | 2001-01-09 |
JP4339404B2 JP4339404B2 (ja) | 2009-10-07 |
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JP50938798A Expired - Fee Related JP4339404B2 (ja) | 1996-08-09 | 1997-08-08 | 線虫及び/又はアリマキに対する耐性 |
JP2008126165A Withdrawn JP2008289481A (ja) | 1996-08-09 | 2008-05-13 | 線虫及び/又はアリマキに対する耐性 |
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US (1) | US6613962B1 (ja) |
EP (2) | EP1493817B1 (ja) |
JP (2) | JP4339404B2 (ja) |
KR (1) | KR20000029896A (ja) |
AT (2) | ATE279523T1 (ja) |
AU (1) | AU735063B2 (ja) |
BR (1) | BR9711048B1 (ja) |
CA (1) | CA2262411C (ja) |
DE (2) | DE69740090D1 (ja) |
DK (2) | DK0937155T3 (ja) |
ES (1) | ES2231891T3 (ja) |
HU (1) | HUP9902900A3 (ja) |
IL (1) | IL128198A (ja) |
MX (1) | MX235300B (ja) |
NZ (1) | NZ334077A (ja) |
PL (1) | PL191777B1 (ja) |
PT (2) | PT937155E (ja) |
RU (1) | RU2221044C2 (ja) |
TR (1) | TR199900277T2 (ja) |
WO (1) | WO1998006750A2 (ja) |
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WO2015002318A1 (ja) * | 2013-07-05 | 2015-01-08 | タキイ種苗株式会社 | トマト植物のネコブセンチュウ抵抗性マーカー、ネコブセンチュウ抵抗性トマト植物、ネコブセンチュウ抵抗性トマト植物の製造方法、およびネコブセンチュウ抵抗性トマト植物のスクリーニング方法 |
WO2022176971A1 (ja) * | 2021-02-19 | 2022-08-25 | 国立大学法人 熊本大学 | 植物寄生性センチュウ防除剤 |
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EP1493817B1 (en) * | 1996-08-09 | 2010-12-29 | Keygene N.V. | Resistance against plant pests |
CN1266457A (zh) * | 1997-06-18 | 2000-09-13 | 麒麟麦酒株式会社 | 抗真菌植物和获得抗真菌植物的方法 |
GB9827152D0 (en) * | 1998-07-03 | 1999-02-03 | Devgen Nv | Characterisation of gene function using double stranded rna inhibition |
US7897843B2 (en) | 1999-03-23 | 2011-03-01 | Mendel Biotechnology, Inc. | Transcriptional regulation of plant biomass and abiotic stress tolerance |
JP2002533379A (ja) | 1998-12-23 | 2002-10-08 | イデア アクチェンゲゼルシャフト | 生体内における局所的に非侵襲性である用途のための改善された製剤 |
FR2849863B1 (fr) * | 2003-01-13 | 2008-02-22 | Genoplante Valor | Gene de resistance a aphis gossypii |
MXPA06001622A (es) | 2003-08-11 | 2006-07-03 | Kweek En Researchbed Agrico Bv | Plantas resistentes a hongos y sus usos. |
AR060563A1 (es) | 2006-04-24 | 2008-06-25 | Mendel Biotechnology Inc | Promotores inducibles por enfermedad |
ZA200900024B (en) | 2006-06-29 | 2010-04-28 | Mendel Biotechnology Inc | Improved yield and stress tolerance in transgenic plants |
US8927811B2 (en) | 2006-08-07 | 2015-01-06 | Mendel Biotechnology, Inc. | Plants with enhanced size and growth rate |
CN113444740B (zh) * | 2021-06-10 | 2022-08-09 | 上海交通大学 | 一种可提高大豆蚜虫抗性基因的重组载体及其应用 |
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GB9104617D0 (en) | 1991-03-05 | 1991-04-17 | Nickerson Int Seed | Pest control |
WO1993006239A1 (en) | 1991-09-24 | 1993-04-01 | Keygene N.V. | Selective restriction fragment amplification: a general method for dna fingerprinting |
GB9205474D0 (en) * | 1992-03-13 | 1992-04-29 | Cambridge Advanced Tech | Root knot nematode resistance |
AU697247B2 (en) | 1993-12-24 | 1998-10-01 | Plant Bioscience Limited | Plant pathogen resistance genes and uses thereof |
EP1493817B1 (en) * | 1996-08-09 | 2010-12-29 | Keygene N.V. | Resistance against plant pests |
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WO2015002318A1 (ja) * | 2013-07-05 | 2015-01-08 | タキイ種苗株式会社 | トマト植物のネコブセンチュウ抵抗性マーカー、ネコブセンチュウ抵抗性トマト植物、ネコブセンチュウ抵抗性トマト植物の製造方法、およびネコブセンチュウ抵抗性トマト植物のスクリーニング方法 |
US10244691B2 (en) | 2013-07-05 | 2019-04-02 | Takii & Company Limited | Root-knot nematode resistance marker for tomato plant, root-knot nematode resistant tomato plant, production method for root-knot nematode resistant tomato plant, and screening method for root-knot nematode resistant tomato plant |
WO2022176971A1 (ja) * | 2021-02-19 | 2022-08-25 | 国立大学法人 熊本大学 | 植物寄生性センチュウ防除剤 |
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