JP2001238610A - Method for producing modified copra meal - Google Patents

Abstract

PROBLEM TO BE SOLVED: To efficiently and economically isolate mannose by introducing salts in the reaction system. SOLUTION: This method for producing modified copra meal features acting mannanase upon copra meal in the coexistence of salts.

Description

DETAILED DESCRIPTION OF THE INVENTION

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a process for producing a modified copra meal containing mannose, which is expected to have a salmonella eradication effect, especially for feed or feed addition.

[0001]

2. Description of the Related Art It has been conventionally known that the addition of mannoses to a feed is expected to have an effect of eliminating Salmonella bacteria (Poultly Science 1989 68 1357).
It has also been known that copra meal, which is produced as coconut oil pulp, is rich in mannan.Mannose polysaccharides obtained by the action of enzymes on raw materials containing galactomannans such as copra meal are also known. It has been reported that blending it with feed for livestock is effective in preventing Salmonella contamination (Japanese Patent Application Laid-Open No. 8-173055).

However, these enzymes use β-mannosidase or galactomannanase alone or in combination with α-galactosidase, and the target component is mannose oligosaccharide or polysaccharide, not mannose. Mannoses differ in efficacy between monosaccharides, oligosaccharides, and polysaccharides, and there are reports that the monosaccharide mannose works most effectively against Salmonella, but mannose is expensive and must be added to feed for poultry farming. It is an economic burden for the industry. Under these circumstances, there is a need in the market to provide a more inexpensive feed-adding mannose in consideration of the effects and production costs.

[0003]

SUMMARY OF THE INVENTION Copra meal is produced as coconut oil squeezed cake, and is itself used as animal feed. However, copramannan, which is contained therein in an amount of 30 to 36%, is indigestible and has little effect on eradicating Salmonella. The present inventors have found that when salts such as NaCl and MgCl2 are added when mannanase is allowed to act on copra meal, a larger amount of monosaccharide mannose is released than when no salts are used, and the present invention has been completed. .
That is, the present invention, in copra meal, in the presence of salts,
This is a method for producing modified copra meal, which is characterized by allowing mannanase to act.

The mannanase to be used is commercially available (for example, hemicellulase GM "Amano" / manufactured by Amano Pharmaceutical Co., Ltd. (trade name), Sumiteam ACH / Shin Nippon Chemical Co., Ltd. (trade name), cellulosin GM5 / Hankyu Bio-Industry Co., Ltd. (trade name) is good. still,
Most of these commercially available products are Aspergillu
s niger.

[0005] These enzymes act on copra meal in the presence of salts. The salt is preferably a chloride salt or a sulfate salt, and a chloride salt or a sulfate salt of an alkali metal or an alkaline earth metal such as NaCl, MgCl2, or MgSO4, or NH4Cl is easily available, price, It is more preferable from the viewpoint of safety. It is convenient to dissolve these salts in the enzyme solution in advance, and in the enzyme solution,
01 to 2.0 M (mol / liter), preferably
It should be adjusted to be 0.005 to 0.5M. 2.0 M
If it exceeds, the effect will be poor.

[0006] Copra meal can be used as a residue produced by squeezing palm oil or as a residue obtained by extracting oils and fats with a solvent, and usually contains mannan in an amount of about 30 to 36% by weight. The enzyme solution containing salts may be mixed uniformly with copra meal, but since copra meal has a very high water absorption, a considerable amount of water is required in the reaction system necessary for uniform mixing. However, it is necessary to remove the water in the mannose purification step and the like, so that the water content should be kept to the minimum necessary.

[0007] Copra meal treated by the present method contains free monosaccharide mannose, and by introducing salts into the reaction system in advance, the inactivation of the enzyme can be suppressed and the reactivity is improved. Copra meal can be particularly effective in eliminating Salmonella by mixing it with the feed as it is or by drying it appropriately or by extracting the water-soluble fraction and adding it to the feed.

For example, 150 g of an enzyme solution obtained by dissolving 15000 u of β-galactomannanase in an aqueous solution of 0.01 M NaCl is added to 100 g of copra meal and reacted at 60 ° C. for 72 hours to produce 12 to 15 g of mannose. The copra meal after the reaction treatment may be directly blended into a dried feed or the like. If the compounding amount is about 0.25 to 1%, the mannose content in the compound feed is 0.01 to 0.075.
% By weight, and an effect of eliminating harmful bacteria such as Salmonella can be obtained.

[0009]

[Example] To 10 g of pressed copra meal, 1/30 g of β-galactomannanase and 15 g of an enzyme solution in which various salts (types and addition amounts are described in Table 1) were added and mixed. And allowed to react at 72 ° C. for 72 hours. The produced mannose was quantified as follows. 50 g of water was added to the enzyme-treated copra meal, and the mixture was kept in a boiling bath for 10 minutes to deactivate the enzyme and simultaneously dissolve the water-soluble component in the aqueous layer. I got The solution was appropriately pretreated, such as by protein removal, and the amount of mannose was determined using ion exchange chromatography. As a result, NaCl (0.001
-0.01M), MgCl2 (0.001-0.1M), CuSO4 (0.001M), etc., produced more mannose than the control (no addition) (Table 1). As the β-galactomannanase, hemicellulase GM “Amano” (trade name, manufactured by Amano Pharmaceutical Co., Ltd., 45000 u / g β-galactomannanase activity) was used.

[0010]

Claims (2)

[Claims] 1. A method for producing a modified copra meal, wherein mannanase is allowed to act on copra meal in the presence of salts. 2. The method according to claim 1, wherein the salt is a chloride salt such as NaCl, MgCl2, NH4Cl or a sulfate salt such as MgSO4.