CN102763652A - Novel chitosan efficient broad-spectrum antibacterial agent - Google Patents

Novel chitosan efficient broad-spectrum antibacterial agent Download PDF

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Publication number
CN102763652A
CN102763652A CN2012101621310A CN201210162131A CN102763652A CN 102763652 A CN102763652 A CN 102763652A CN 2012101621310 A CN2012101621310 A CN 2012101621310A CN 201210162131 A CN201210162131 A CN 201210162131A CN 102763652 A CN102763652 A CN 102763652A
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China
Prior art keywords
shitosan
antibacterial agent
ultra micro
water
novel chitosan
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Pending
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CN2012101621310A
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Chinese (zh)
Inventor
王开宇
王斐斐
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QINGDAO YUNZHOU BIOCHEMISTRY CO Ltd
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QINGDAO YUNZHOU BIOCHEMISTRY CO Ltd
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Priority to CN2012101621310A priority Critical patent/CN102763652A/en
Publication of CN102763652A publication Critical patent/CN102763652A/en
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Abstract

The invention relates to a novel chitosan efficient broad-spectrum antibacterial agent, and is characterized by providing a production technology of a super-fine chitosan, and a formula and a special synthetic technology for the novel chitosan efficient broad-spectrum antibacterial agent. The antibacterial agent is prepared according to strict screening and compatibility based on experiments and through a special process, and convenient for production and usage, has good compound effect, no toxic or side effect on human body, gains functions of efficient broad-spectrum sterilization, damp clearing and skin moisturizing, and can be widely used in disease prevention and treatment and antibiotic disinfection of people to improve and protect the life quality; besides, the antibacterial agent is applicable to disease prevention and treatment for various populations in various fields, health supplies disinfection, disease prevention and treatment in animal husbandry and aquaculture, antibacterial finishing of printing and dyeing textile, and antisepsis and mold removal of fruit and vegetable.

Description

Novel chitosan high-efficiency broad spectrum antibacterial agent
Technical field the present invention relates to a kind of novel chitosan high-efficiency broad spectrum antibacterial agent.The prescription and the special synthesis technique of a kind of ultra micro shitosan production technology, novel chitosan high-efficiency broad spectrum antibacterial agent have been it is characterized in that inventing.At occurring in nature, estimate 10,000,000,000 tons of annual biosynthetic chitins nearly, distribute extensively, comprise in the cell wall of arthropods, mollusk etc. and rudimentary plant such as fungi, algae.In the natural organic matter that exists on earth, chitin is only second to cellulose, also is simultaneously the outer the highest natural organic matter of nitrogen content of isolating protein, it serves to show that it is a kind of inexhaustible, nexhaustible regenerated resources.Shitosan then is that chitin is separated the water-soluble products that deacetylation generates through strong lye, is a kind of important derivatives of chitin.Along with the raising of living standards of the people, the exploitation of wide spectrum, efficient, safe natural anticorrosion antibacterial agent has received people's common concern.Shitosan makes one of its research focus that becomes the exploitation of natural anticorrosion antibacterial agent owing to have characteristics such as biodegradability, biocompatibility, biological nontoxic property and antibacterial activity.The present invention utilizes the chemism site of shitosan that it is carried out chemical modification; Obtained a kind of novel chitosan derivative antibacterial agent around improving antibacterial activity and these two key points of dissolubility, synthesizing---novel chitosan high-efficiency broad spectrum antibacterial agent, synthetic route, product characteristics and the antibacterial activity of broad-spectrum antiseptic functional group chitosan derivatives-bios DH220 and hydrolase are studied.To Escherichia coli, staphylococcus aureus, Candida albicans effect 2 minutes, sterilizing rate was greater than 99.99% through its novel chitosan high-efficiency broad spectrum antibacterial agent of 0.2% of test; Its LD50 is greater than 10g/kg, belongs to actual nontoxic level., skin is not also had obvious irritation and do not present the allergy of skin, be a kind of safe new type natural biological high-efficiency broad spectrum antimicrobicide.In order to improve the water-soluble of shitosan, it has been carried out salify ultra micro film handled, logical ultra micro shitosan has fabulous solute effect under the environment of little acid neutrality that lactic acid is regulated.The prescription of novel chitosan high-efficiency broad spectrum antibacterial agent of the present invention is water-soluble ultra micro shitosan, and bios DH220, hydrolase, organic amine-lactic acid liquid are hydrolyzed to gel in little sour neutral environment.Do not contain any chemical composition; Through the strict screening of experiment compatibility, the compound action effect is good, forms through the special process configuration; Making, easy to use; To the human non-toxic side effect, have the effect of high-efficiency broad spectrum sterilization, clearing damp skin moisten, can be widely used in common people's disease preventing and treating antibiotic and sterilizing; Improve and the protection life quality, be applicable to that the various crowds in various fields prevent and cure diseases, the antibiotic finish of the preventing and curing diseases of antibiotic and sterilizing, livestock-raising and the aquaculture of amenities, dyeing textile article, food fruits and vegetables anticorrosion except that field such as mould.
Background technology people's living standard is along with the development of society improves day by day, and increasing people is with the quickening of rhythm of life, and the pressure that comes from aspects such as society, work, life is increasing; The incidence of disease of various diseases is increasingly high, and people's health care consciousness constantly strengthens in diseases prevention, and disease-prevention health and disinfecting, antimicrobial are caused concern and attention; Therefore; A kind of safe, effective, convenient and practical tegument glycan novel chitosan high-efficiency broad spectrum antibacterial agent of research invention has been corrected the existing thimerosal active ingredient pure chemicals of distinguishing the flavor of, and bactericidal effect and toxicity are directly proportional with content; Cost price is high, poor stability; Be corrosive and penetrating odor, skin is had certain damage, and difficulty is rinsed well.Therefore, people hope to have a kind of can be in sterilization, nourishes rough skin, keep skin normal physiological environment, the thimerosal of high-efficiency broad spectrum pure natural comes out.The object of the present invention is to provide a kind of composite multifunction biological antiseptic liquid that can overcome the existing procucts deficiency; Belonging to nontoxic, non-stimulated, no burn into does not have the green product that allergy, colorless and odorless have no side effect, have disinfecting skin-care, sterilization dispel flavor, good stability, be convenient to flushing, characteristics such as convenient and practical.Because the function demand just according to the invention of shitosan self.The present invention utilizes the chemism site of shitosan that it is carried out chemical modification; Obtained a kind of novel chitosan derivative antibacterial agent around improving antibacterial activity and these two key points of dissolubility, synthesizing---novel chitosan high-efficiency broad spectrum antibacterial agent, synthetic route, product characteristics and the antibacterial activity of broad-spectrum antiseptic functional group chitosan derivatives-bios DH220 and hydrolase are studied.To Escherichia coli, staphylococcus aureus, Candida albicans effect 2 minutes, sterilizing rate was greater than 99.99% through its novel chitosan high-efficiency broad spectrum antibacterial agent of 0.2% of test; Its LD50 is greater than 10g/kg, belongs to actual nontoxic level., skin is not also had obvious irritation and do not present the allergy of skin, be a kind of safe new type natural biological high-efficiency broad spectrum antimicrobicide.In order to improve the water-soluble of shitosan, it has been carried out salify ultra micro film handled, logical ultra micro shitosan has fabulous solute effect under the environment of little acid neutrality that lactic acid is regulated.The prescription of novel chitosan high-efficiency broad spectrum antibacterial agent of the present invention is water-soluble ultra micro shitosan, and bios DH220, hydrolase, organosilicon quaternary ammonium salt CH168, organic amine-lactic acid liquid are hydrolyzed to gel in little sour neutral environment.Do not contain any chemical composition, through the strict screening of experiment compatibility, the compound action effect is good, form through the special process configuration,
Summary of the invention main points of the present invention are the chitin of occurring in nature is carried out extraction process in two steps; The first step removes calcium carbonate with watery hydrochloric acid: second step removed protein with hot diluted alkaline; Handle the chitin that just can obtain white through decolouring again, between the conversion ratio general 15~30%.With chitin alkalization insulation deacetylation, clean to neutrality and be shitosan, through special mocromembrane nanometer technology get final product the ultra micro shitosan.The ultra micro shitosan can suppress pathogen and grow, and does not influence the balance of natural ecological, because of containing free amine group; Be alkaline polysaccharide unique in the natural polysaccharide, have many special physics and chemical property and physiological function, can combine acid molecule; Generating amino cation makes it become positively charged natural polymer; Its antibacterial action mainly is a shitosan band positive electricity property, can combine with electronegative part in the bacterioprotein, and bacterium or fungi are lost activity.The shitosan of small-molecular weight may penetrate into microorganism inside in addition, stops RNA (ribonucleic acid) to transform, thereby suppresses the growth of bacterium. because the application of shitosan and derivative thereof is increasingly extensive, so its safety has also caused people's serious concern.The safety of ultra micro shitosan; Carried out many-sided research, these researchs relate to acute toxicity experience, subacute toxicity test, teratogenesis and carcinogenicity test, skin irritant experiment, skin accumulation irritation test, toxicity test, skin anaphylactic test, the test of eye mucosa irritation property, skin absorption test etc. for the first time.The result shows that the ultra micro shitosan belongs to actual nontoxic level.Why shitosan and derivative thereof can be antibacterial, mainly are because their right cytoplasma membranes have played certain function, have destroyed its normal physiological function.However, still there are some differences in shitosan and its derivative on antibiotic mechanism.
Electron microscopic observation is found, after bacterium receives the shitosan effect, tangible metamorphosis can take place, cell attenuation even the breakage of grampostive bacteria such as staphylococcus aureus, and the effect of duplicating is suppressed; Gram-negative bacteria such as colibacillary cytoplasm take place to concentrate, and the gap between the matter wall obviously enlarges.This shows that shitosan has played obvious suppression or destruction to the cell growth of bacterium.The somebody thinks that shitosan dissolving back belongs to polycation; Thereby interactions such as the acidic materials that can produce with bacterium surface such as lipopolysaccharides, techoic acid, teichuronic acid, capsular polysaccharide; Form complicated polyelectrolyte, thereby the cell membrane function of bacterium is got muddled.Antibiotic property mechanism because hydrophobic affinity interaction takes place between the phosphatide on basic chain of hydrophobic length and the bacterial cell plasma membrane, has been destroyed the bacterial cell plasma membrane, from then having produced antibiotic property.Also there are bigger difference in bios and shitosan on antibiotic mechanism.Have report to think, bios can hinder DNA to the transcribing of RNA, and influence the growth and breeding of bacterium, thus the generation antibacterial action.And shitosan acts on a kind of polyphosphazene polymer cation, gathers at the cell wall place easily, prevents that nutriment from passing through cell wall and getting into cell, makes cell failure dead.
Novel chitosan high-efficiency broad spectrum antiseptic prescription of the present invention is (component and percentage by weight % thereof) as follows: water-soluble ultra micro shitosan 3-5; Bios DH220 1-2; Hydrolase 0.5-0.8; Organic amine-lactic acid liquid 0.5-1; Dual-filtering water to 100.
The technological process of the water-soluble ultra micro shitosan of the present invention is as follows: shrimp and crab shells → select → wash → acidleach 4~6%HCl room temperature 5h decalcification → soda boiling 5-10%NaOH, and the above-mentioned acidizing alkali metallization processes time of 80-100 ℃ of 3h deproteinization → repeat is respectively 3h, 2h → decolouring KMnO4/ oxalic acid → drying → chitin; Chitin 40~50%NaOH100 ℃ of 16h → insulation deacetylation → clean to neutrality → 80-100 ℃ of heated-air drying → shitosan → special mocromembrane nanometer technology → ultra micro shitosan; Little sour neutral solution with 3-5% ultra micro shitosan is the effective ingredient-water soluble chitosan of shitosan biogel finishing agent prescription.
The synthesis technique of novel chitosan high-efficiency broad spectrum antibacterial agent of the present invention is following: water-soluble ultra micro shitosan 3-5; Bios DH220 1-2; Hydrolase 0.5-0.8; Organic amine-lactic acid liquid 0.5-1; Dual-filtering water to 100.Stirring is mixed gets final product in dissolving under 60 ℃ of conditions of ratio separately.
The present invention has the following advantages: pure natural shitosan elite composition is refined in (1), and is nontoxic to human body skin; Environmental protection, nontoxic.(2) biological degradability.Good biocompatibility has good spectrum antibacterial antiplaque performance and good moisture pick-up properties.(3) have sterilizing, clearing damp skin moisten effect.(4) its making, easy to use, safety has no side effect, persistent, long shelf-life.(5) through experiment showed, that effective ingredient is stable, germicidal efficiency is high, and fungicidal spectrum is wide, to the sterilizing rate of Escherichia coli, staphylococcus aureus, Candida albicans more than 99.99%.
Embodiment: the present invention is done further detailed description below in conjunction with embodiment:
1. carry out the synthetic of water-soluble ultra micro shitosan according to the technological process of the water-soluble ultra micro shitosan of the present invention: shrimp and crab shells → select → wash → acidleach 4~6%HCl room temperature 5h decalcification → soda boiling 5-10%NaOH, the above-mentioned acidizing alkali metallization processes time of 80-100 ℃ of 3h deproteinization → repeat is respectively 3h, 2h → decolouring KMnO4/ oxalic acid → drying → chitin; Chitin 40~50%NaOH100 ℃ of 16h → insulation deacetylation → clean to neutrality → 80-100 ℃ of heated-air drying → shitosan → special mocromembrane nanometer technology → ultra micro shitosan; Little sour neutral solution with 3-5% ultra micro shitosan is the effective ingredient-water-soluble ultra micro shitosan of novel chitosan high-efficiency broad spectrum antiseptic prescription.
2. the synthesis technique according to novel chitosan high-efficiency broad spectrum antibacterial agent of the present invention carries out synthesizing new shitosan high-efficiency broad spectrum antibacterial agent: listed data of following table (percentage by weight %) and said step thereof are configured: with water-soluble ultra micro shitosan 3-5; Bios DH220 1-2; Hydrolase 0.5-0.8; Organic amine-lactic acid liquid 0.5-1; Dual-filtering water to 100.Stirring is mixed gets final product in dissolving under 60 ℃ of conditions of ratio separately.

Claims (3)

1. the present invention relates to a kind of novel chitosan high-efficiency broad spectrum antibacterial agent.It is characterized in that having following prescription (component and percentage by weight % thereof): water-soluble ultra micro shitosan 3-5; Bios DH220 1-2; Hydrolase 0.5-0.8; Organic amine-lactic acid liquid 0.5-1; Dual-filtering water to 100.
2. the said novel chitosan high-efficiency broad spectrum of claim 1 antibacterial agent is characterized in that having preparation technology's flow process of following water-soluble ultra micro shitosan:
Shrimp and crab shells → select → wash → acidleach 4~6%HCl room temperature 5h decalcification → soda boiling 5-10%NaOH, the above-mentioned acidizing alkali metallization processes time of 80-100 ℃ of 3h deproteinization → repeat is respectively 3h, 2h → decolouring KMnO4/ oxalic acid → drying → chitin; Chitin 40~50%NaOH100 ℃ of 16h → insulation deacetylation → clean to neutrality → 80-100 ℃ of heated-air drying → shitosan → special mocromembrane nanometer technology → ultra micro shitosan; Little sour neutral solution with 3-5% ultra micro shitosan is the effective ingredient-water-soluble ultra micro shitosan of novel chitosan high-efficiency broad spectrum antiseptic prescription.
3. the said novel chitosan high-efficiency broad spectrum of claim 1 antibacterial agent is characterized in that having following synthesis technique:
With water-soluble ultra micro shitosan 3-5; Bios DH220 1-2; Hydrolase 0.5-0.8; Organic amine-lactic acid liquid 0.5-1; Dual-filtering water to 100.Stirring is mixed gets final product in dissolving under 60 ℃ of conditions of ratio separately.
CN2012101621310A 2012-05-24 2012-05-24 Novel chitosan efficient broad-spectrum antibacterial agent Pending CN102763652A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103109797A (en) * 2013-02-26 2013-05-22 广州市锐博生物科技有限公司 Chitosan nano particle, biological pesticide preparation for controlling beet armyworms and preparation method thereof
CN104770404A (en) * 2015-03-18 2015-07-15 陈斌 Preparation method of toothbrush disinfectant solution
CN109691449A (en) * 2017-10-23 2019-04-30 青岛胜恩全成生物科技有限公司 A kind of fungicide and preparation method thereof for preventing and treating large cherry tree bleeding disease

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103109797A (en) * 2013-02-26 2013-05-22 广州市锐博生物科技有限公司 Chitosan nano particle, biological pesticide preparation for controlling beet armyworms and preparation method thereof
CN103109797B (en) * 2013-02-26 2015-04-08 广州市锐博生物科技有限公司 Chitosan nano particle, biological pesticide preparation for controlling beet armyworms and preparation method thereof
CN104770404A (en) * 2015-03-18 2015-07-15 陈斌 Preparation method of toothbrush disinfectant solution
CN109691449A (en) * 2017-10-23 2019-04-30 青岛胜恩全成生物科技有限公司 A kind of fungicide and preparation method thereof for preventing and treating large cherry tree bleeding disease

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Application publication date: 20121107