CN103875666A - Degerming composition and application thereof - Google Patents

Degerming composition and application thereof Download PDF

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Publication number
CN103875666A
CN103875666A CN201210554611.1A CN201210554611A CN103875666A CN 103875666 A CN103875666 A CN 103875666A CN 201210554611 A CN201210554611 A CN 201210554611A CN 103875666 A CN103875666 A CN 103875666A
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acid
bacteria composition
chitose
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composition
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魏亚中
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魏亚中
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Abstract

The invention relates to a degerming composition, comprising chitose and a peroxy organic acid. A ratio (w/w) of the chitose to the peroxy organic acid is 0.01-100. The chitose is one or more composition selected from a group consisting of amino monosaccharide, amide monosaccharide, chitosan oligosaccharide, chitosan; and the peroxy organic acid is a C1-C18 aliphatic peroxy organic acid that is one or more composition selected from a group consisting of peroxy monocarbonic acid and peroxy bicarbonic acid. The degerming composition is used for protecting an object from damages brought by microbes. The degerming composition has the effects of protecting various materials and preventing damages brought by the microbes.

Description

Except bacteria composition and application thereof
Technical field
The present invention relates to a kind of bacteria composition that removes, relate in particular to for the protection of various materials, as natural material, artificial material, organism etc., avoid suffering the degerming protectant of microorganism injury.
Background technology
Since ancient times, no matter be organism or abiotic object, under atmospheric environment, all can face implantation and the breeding that microorganism comprises bacterium, mould, fungi etc.Microbes grows cause of disease, and injury human body, animal and plant, cause public health problem; Microbial growth and metabolism, can produce stink affects air quality, also can produce acid compound and cause the degraded of material and aging, and can chromogenesis pollute the outward appearance of object.Therefore,, for avoiding object to suffer the injury of microorganism, be necessary to implement degerming safeguard measure.Medicament for degerming is commonly referred to as antibacterial agent, can divide into bacteriostatic agent (antimicrobials), bactericide (Disinfectants), disinfectant (Sanitizers) etc. according to degerming mode and degerming speed.The antibacterial agent of existing use is very many, be divided into inorganic matter, organic matter and natural goods three major types system, all there is toxicity problem in general antibacterial agent, especially the extraordinary inorganic compound of bacteria-eliminating efficacy or organic compound, such as halide, heavy metal, hydroformylation thing, phenolate thing etc. are for degerming, in air, living space, article surface all can leave residual hazard, affects human and livestock health.
Chitose is on the earth, to contain abundant natural polymer, the resource that existing raw material is mainly taken from aquatic products shell discarded object reclaims, aminated polysaccharide body (Aminopolysaccharide) chemical constitution of chitose has been proved has antibacterial functions, document about chitose antibacterial test is delivered, show that it can effectively suppress various microbial growths widely, comprising: bacterium, mould, fungi, saccharomycete, algae.The chemical structural formula of chitose is as follows:
Amido monose amide groups monose
Physical property, voltinism and the biological property of chitose, depend mainly on two microstructure parameters: " molecular weight MW " (molecular weight) and " Deacetylation degree DD " (degree ofdeacetylation, DD=m/(m+n)), chitose is water insoluble, but in the dilute acid solution of pH<6.3, the amido-NH of chitose 2can change into-NH 3 +dissolve and produce.
The action principle that suppresses growth of microorganism about chitose, has three kinds of discussions.First discussion is widely accepted most, advocate that the positive charge of chitose molecule and the negative electrical charge of microorganism wall produce electrostatic force, this electrostatic interaction causes two interference for microorganism: (1) cell wall permeability of the membrane changes, cause cell interior osmotic pressure imbalance, (2) the victory peptide glycan of cell wall produces hydrolysis, the electrolyte that causes cell interior leaks, and facilitates inhibition growth of microorganism.Second discussion, advocates the cell wall of micromolecular chitose through microorganism, and the DNA that enters interference cell core in cell is transcribed into RNA, stops the synthetic of protein.The 3rd discussion, advocates the chitose molecule chelation for metal, subdues the generting element of spore, and the restriction micro-organisms needed nutrient of growing.
Above-listed three kinds of discussions, have all obtained experiment showed, that experimental data shows that the antibacterial effect of chitose is determined by molecular weight and Deacetylation degree.Amido-the NH of chitose 2provide electrostatic interaction and chelation, the internal factor by chemical constitution: Deacetylation degree DD is determined, and is subject to extrinsic factor: the impact of conjugate acid and pH value.Experimental data also shows that the antibacterial effect of chitosan oligosaccharide (oligomer) is better than shitosan (polymer), and molecular weight ratio Deacetylation degree is more important for the impact of antibacterial effect.
Chitose has been made into various antibacterial formulation, and the antibacterial carrier such as fiber, film, compound, is applied to each field; Wherein, chitose suppresses growth of microorganism, and speed of action is slow, belongs to bacteriostatic agent, because can not quick sterilization, and there is no effect for general virus, cannot serve as disinfectant.
Peroxide organic acid (peroxycarboxylic acid) is taking organic acid and hydrogen peroxide as raw material, carry out the product that peroxide reaction (perhydrolysis) obtains, this is a reversible reaction, when peroxide organic acid is dissolved in water, can pass through reverse reaction, produce oxidability, change into hydrogen peroxide, organic acid, and further resolve into end product: O 2, CO 2, H 2o.Peroxide organic acid industrial in a large number for sterilization, bleaching, synthetic fine chemicals.
Peroxide organic acid is implemented sterilization with oxidation, discharge electronics and reach quick sterilization to microorganism, with Peracetic acid (peroxyacetic acid, PAA) be example, its oxidizing force (1.81eV) exceeds clorox (1.36eV), can eliminate bacterium, mould, fungi, virus etc., in environment, leave nontoxic and water-soluble product, there is no the residual toxicity problem of the oxidizing biocides such as clorox.Peracetic acid possesses that quick sterilization, effect effect are extensive, the property of medicine stable, the nontoxic characteristic such as residual, obtains WHO, US FDA, the US EPA accreditation disinfectant as safety non-toxic, is used in the fields such as medicine, food, environment.
Use peroxide organic acid to make degerming disinfectant, normally, taking peroxide organic acid, hydrogen peroxide, water three as basic ingredient, separately add some adjuvants, form composition. add organic hypophosphorous acid (organic phosphonicacid) seizure metal ion Deng people (US4051058 and US4051059), and add the teepol enhancement stability of sulfuric acid system or sulfonic acid system.The people such as Crommelynck (US4297298) add micro-strong acid and promote stability.The people such as Jourdan-Laforte (US4587264) add nitric acid and reach anticorrosive.The people such as Casentino (US5508046 and US5656302) use hypophosphorous acid and sodium pyrophosphate (sodiumpyrophosphate), and add divalence and trivalent ion enhancement stability.The people such as Melone (US5720983) use alkali-metal phosphate anticorrosive with Dipotassium pyrophosphate, and add phosphoric acid or phosphate enhancement stability.The people such as Smith (US20040143133) add amine oxide (aminooxide) as deodorant.
About the two the combination of peroxide organic acid and chitose, prior art application peroxide organic acid is made oxidation chitose as oxidant, or for reducing the viscosity of chitose, or for adjusting pH-value.The people such as Eoff (US6764981, US6981552 and US7007752) disclose oxidation chitose for drilling well, use Peracetic acid as oxidant, degrade and oxidation for chitose, make the oxidation chitose that water-soluble height and viscosity are low.The people such as Wick (US6794346) disclose the composition of chitose and furnanone for cleaning agent, in order to allow chitose and furnanone performance optimum efficiency, use lactic acid, sulfamic acid, citric acid, valeric acid, caproic acid, glycolic to adjust pH-value to pH<5, mention that Peracetic acid is the sour composition being applicable to.The composition of the people such as Avery (US6849586) announcement chitose and other (organic or inorganic) chemical antibacterial agents is soluble in water for cleaning agent, in order to allow chitose performance optimum efficiency, use organic acid to adjust the pH-value pH<7 of the aqueous solution, mention that Peracetic acid is feasible organic acid.
As for peroxide organic acid and chitose the two in conjunction with being applied to degerming, existing technology is not pure use this two as effective antibacterial composition, and not by this two and with carrying out direct action.Chitose, acid, other antibacterial agents are listed as necessary composition by aforementioned published patent formulation (US6794346 and US6849586), use peroxide organic acid and chitose another antibacterial agent in addition as degerming active ingredient, import the problem that another antibacterial agent will definitely cause safety and residual hazard, limit its range of application.The people such as Narciso (J.A.Narciso, E.A.Baldwin, A.Plotto, and C.M.Ference, Preharvest Peroxyacetic Acid Sprays Slow Decay and ExtendShelf Life of Strawberries, Hort Science42(3): 617-621.2007) first use peroxide organic acid to spray for the strawberry before gathering, strawberry after gathering is soaked with chitose solution again, reach degerming and fresh-keeping effect, peroxide organic acid and chitose divide at two program effects, limit its convenience and increase cost.
Therefore, the property improved of the above-mentioned defect of inventor's thoughts, spy concentrates on studies and coordinates the utilization of scientific principle, finally proposes a kind of reasonable in design and effectively improve the present invention of above-mentioned defect.
Summary of the invention
The invention provides a kind of except bacteria composition and application thereof, in air, living space, article surface can not leave residual hazard, harmless in human and livestock health.
The bacteria composition that removes that comprises chitose (chitosan) and peroxide organic acid (peroxycarboxylic acid) except bacteria composition provided by the invention has sterilization, antibacterial, except effect of spot, this is one preferably except bacteria composition, the two composition ratio of its chitose and peroxide organic acid: chitose/peroxide organic acid (w/w)=0.01-100, chitose is selected from amido monose, amide groups monose, chitosan oligosaccharide, shitosan forms one or more compositions in group, peroxide organic acid is the aliphatic peroxide organic acid of C1-C18, it is selected from the acid of peroxide list carbonyl, two carbonyl one or more compositions in group that acid forms of peroxide, wherein, should remove the degerming liquor that bacteria composition can use solvent dilution to become to contain >10ppm chitose.
In other words, the bacteria composition that removes provided by the invention comprises chitose and peroxide organic acid, should be except the two composition ratio of the chitose in bacteria composition and peroxide organic acid: chitose/peroxide organic acid (w/w)=0.01-100, chitose is selected from amido monose, amide groups monose, chitosan oligosaccharide, shitosan and forms one or more compositions in group, peroxide organic acid is the aliphatic peroxide organic acid of C1-C18, and it is selected from the acid of peroxide list carbonyl, two carbonyl one or more compositions in group that acid forms of peroxide; Wherein, should can be diluted to the bacteria remover that contains >10ppm chitose except bacteria composition.The present invention also provides a kind of above-mentioned application except bacteria composition, should keep out the injury of microorganism for the protection of object except bacteria composition.
The beneficial effect that the present invention has:
Of the present invention except bacteria composition, its peroxide organic acid and chitose are the not safe antibacterial agent of residual toxicity, peroxide organic acid possesses the quick-acting sterilizing power of wide spectrum, chitose possesses the feature of long acting antibiotic and excellent film forming, the two has complementary functions, and is applicable to composition safety, convenient, efficient degerming protectant; Its peroxide organic acid and chitose have compatibility, stability and the splendid advantage such as stability and degerming synergistic effect of height; Its peroxide organic acid and chitose can disposablely complete, and do not need to mix other antibacterial compositions and can form stable except bacteria composition, so have the advantages such as the Production Time of saving and cost; It can concentration on demand be made into the aqueous solution, acts on hard or soft object, reach sterilization, fungi-proofing, except spot function, and can form degerming diaphragm at body surface.
For enabling further to understand feature of the present invention and technology contents, refer to following about detailed description of the present invention and accompanying drawing, but appended accompanying drawing only provide with reference to and explanation use, be not used for the present invention to be limited.
Brief description of the drawings
Fig. 1 is the degerming flow chart that the present invention removes bacteria composition and act on body surface;
Fig. 2 A is the degerming schematic diagram () that the present invention removes bacteria composition and act on body surface;
Fig. 2 B is the degerming schematic diagram (two) that the present invention removes bacteria composition and act on body surface;
Fig. 2 C is the degerming schematic diagram (three) that the present invention removes bacteria composition and act on body surface.
[main element description of reference numerals]
1 body surface
2 degerming diaphragms
Step S101 is to step S103
Embodiment
The present invention relates to the bacteria composition that removes derived from chitose (chitosan), the more important thing is with chitose (chitosan) and the combination of peroxide organic acid (peroxycarboxylic acid) and strengthen sterilization and antibacterial effect.Preferably except bacteria composition is the two composition ratio of chitose and peroxide organic acid: chitose/peroxide organic acid (w/w)=0.01-100; The degerming liquor that wherein, also can use solvent dilution to become to contain >10ppm chitose except bacteria composition.
The present invention takes edible natural goods: chitose (chitosan), as except one of them principal component of bacteria composition, its chemical composition is not had toxicity, and be the safe antibacterial agent that Environmental Protection Agency's (being called for short USEPA) is approved.Another principal component except in bacteria composition: peroxide organic acid (peroxycarboxylic acid), also be the safe antibacterial agent of USEPA accreditation, because peroxide organic acid is carried out after degermation, can change into organic acid and be combined with chitose and form salt, so residual toxicity material not.
Chitose (chitosan) is natural polysaccharide body (polysaccharide), two kinds of construction units of its macromolecular chain amino-contained monose and amide groups monose, and film forming is very good, and can suppress microbial growth.Chitose is water insoluble, but be dissolved in some organic acids and inorganic aqueous acid, the molecular weight of chitose and Deacetylation degree determine its film forming, the film forming of chitose is also limited by the performance of acid molecule in solution, in the time that the chemical constitution specification of chitose immobilizes, use dissolubility acid better, can obtain physical property chitose film better.Chitose is dissolved in aqueous acid, and its principle can represent with reaction equation one: the amido-NH of chitose 2receive the free protons H in water +, change into-NH 3 +dissolve and produce.
Reaction equation one:
-NH 2+H ++A →-NH 3 ++A
The present invention use peroxide organic acid and chitose the two form except bacteria composition, due to the product (organic acid) of peroxide organic acid and its decomposition, in water, dissociate proton H +, as shown in reaction equation two, so very good for the dissolubility of chitose, can reach even and stable chitose solution, and obtain excellent film forming, in other words, due to of the present invention except two principal component peroxide organic acids (RCOOOH) in bacteria composition and chitose (Ch-NH 2) form ion pair, thus there is the compatible of height and be stable homogeneous phase, as shown in reaction equation three:
Reaction equation two:
Reaction equation three:
Ch-NH 2+RCOOOH→Ch-NH 3 ++RCOOO -
The antibacterial effect of chitose is by the internal factor of chemical constitution: Deacetylation degree and molecular weight are determined, and be subject to extrinsic factor: the impact of conjugate acid and pH value.As shown in above-mentioned reaction equation three, peroxide organic acid organic acid and free protons H corresponding with it in water +, form balance, stable conjugate acid and pH value environment is provided, impel chitose to bring into play excellent antibacterial effect, in other words, of the present inventionly remove peroxide organic acid and chitose in bacteria composition and there is splendid stability and splendid degerming synergistic effect.
Following examples are of the present invention illustrating, but can therefore not limit protection scope of the present invention.The chitose that the present invention uses except bacteria composition can be selected from amido monose, amide groups monose, chitosan oligosaccharide, shitosan and form one or more compositions in group; Preferably, chitose is selected chitosan oligosaccharide.The molecular weight of chitose of impact about to(for) antibacterial effect, experimental data shows that the antibacterial effect of chitosan oligosaccharide (oligimer) is better than shitosan (polymer), and molecular weight ratio Deacetylation degree is even more important for the impact of antibacterial effect.Chitosan oligosaccharide is the product through depolymerization reaction (Depolymerization) by shitosan, and chitose can be by three kinds of modes: chemical method, Physical, ferment method are carried out depolymerization.Ferment method is used chitinase (chitosanase) or protease (protease) to be hydrolyzed (hydrolysis) for shitosan, obtains chitosan oligosaccharide.Physical uses ultrasonic (ultrasonication) or radiation (irradiation) to carry out depolymerization.Chemical method use acid be hydrolyzed reaction (acidic hydrolysis) or use oxidant carry out oxidative degradation (oxidativedegradation), cut-out shitosan high polymer main chain, obtain low-molecular-weight chitosan oligosaccharide.The oxidative degradation of chitose can adopt the oxidants such as peroxide, natrium nitrosum, ozone, oxidant can act on two positions of sugared structure for the oxidation reaction of chitose: the functional group on the link button of six interannulars or six rings, the six ring structure unit that the former maintains chitose are constant, the length that only reduces molecular backbone, is called degradation reaction; The latter produces open loop (ring-opening), carbonylation (carboxylation), amino loss (loss ofamino group), is considered as the side reaction of degradation reaction; Use peroxide to carry out oxidative degradation, via reaction condition: concentration, temperature, acid-base value, isoparametric control of time, can suppress the generation of side reaction, obtain chitosan oligosaccharide.
The aliphatic peroxide organic acid that is C1-C18 at the peroxide organic acid using except bacteria composition of the present invention, it is selected from the acid of peroxide list carbonyl, two carbonyl one or more compositions in group that acid forms of peroxide; Wherein, because peroxide organic acid can produce organic acid and hydrogen peroxide in water, form chemical balance, wherein peroxide organic acid and hydrogen peroxide are oxidant, and oxidability is provided, and impel chitose to produce oxidative degradation, promote the antibiotic property of chitose.It is worth mentioning that, of the present invention except bacteria composition can be further according to the demand of application and performance, flexible adjustment removes the parameters such as incorporation time, mixing temperature and the hybrid mode of peroxide organic acid concentration, peroxide organic acid proportioning, peroxide organic acid and chitose in bacteria composition, to improve dissolubility and to change viscosity, so can prepare most suitable except bacteria composition; Preferably, of the present inventionly can contain 0.01-30%(w/w except bacteria composition) the organic acid of C1-C18; Preferably, of the present inventionly can contain 0.01-30%(w/w except bacteria composition) hydrogen peroxide; Preferably, of the present inventionly can contain 0.01-30%(w/w except bacteria composition simultaneously) hydrogen peroxide and 0.01-30%(w/w) the organic acid of C1-C18.Above-mentioned organic acid can be selected from formic acid, acetic acid, propionic acid, sad, capric acid, lactic acid, glutaric acid, adipic acid, decanedioic acid, citric acid, malic acid, maleic acid, gluconic acid, amino acid forms one or more compositions in group.
The degerming liquor that can use solvent dilution to become to contain >10ppm chitose except bacteria composition of the present invention; The wherein said bacteria composition that removes can be diluted with water to the uniform aqueous solution, more specifically, because proposed by the invention can be completely water-soluble except bacteria composition, so when the chitose concentration in the aqueous solution is at 10ppm-10%(w/w) time, composition for a long time (>6 month) exists with even aqueous stabilizing.On the other hand, what chitose and peroxide organic acid formed removes bacteria composition, while acting on body surface with the form of the aqueous solution, due to hydrone and gas molecule generation evaporation, the remnant that therefore remains in body surface can form the diaphragm taking chitose as skeleton basis; Wherein, as shown in reaction equation four, the composition that chitose and peroxide organic acid form is at the degermation of body surface, peroxide organic acid peroxy (RCOOO -) can discharge acid radical anion (RCOO -) and free oxygen base ([O]), free oxygen base ([O]) is carried out quick degerming, and remaining acid radical anion (RCOO -) with chitose cation (Chitosan-NH 3 +) formation chitose salt (Chitosan-NH 3 +-OOCR) and be fixed in diaphragm, so can make to continue to exercise the function of long-acting degerming except bacteria composition, as shown in reaction equation five.
Reaction equation four:
RCOOO →RCOO +[O]
Reaction equation five:
Chitosan-NH 3 ++RCOO →Chitosan-NH 3 +OOCR
Ch-NH 3 ++RCOO →Ch-NH 3 +OOCR
In sum; of the present invention except bacteria composition not only optionally concentration be made into the aqueous solution or emulsified dispersed liquid; act on hard or soft object, can also form degerming diaphragm at body surface except bacteria composition, with reach sterilization, fungi-proofing, except the function of spot.In addition, the present invention also can be by means of parameters such as incorporation time, mixing temperature and the hybrid modes of adjustment peroxide organic acid concentration, peroxide organic acid proportioning, peroxide organic acid and chitose, to improve the performance of diaphragm.
Because form that can liquid except bacteria composition (being chitose and peroxide organic acid composition) of the present invention stores, using as degerming purposes, for extending period of validity of the present invention, the present invention can further add and has the auxiliary element of promoting stability.For instance, do not affecting under bacteria-eliminating efficacy of the present invention, the present invention can add by compound or stabilization agent compatible and effective dose test, to prepare homogeneous liquid; Wherein, described compound or stabilization agent have the effect that makes peroxide stable.Preferably, the content of the stabilization agent that the present invention adds can be 10ppm-10%(w/w), and stabilization agent can be selected from stannate, pyrophosphate, silicate, sulphate, sulfonate and forms one or more compositions in group.Described stabilization agent not only capable of regulating peroxide organic acid dissociates proton H +degree, impel chitose to bring into play best antibacterial effect, also can make chitose evenly and unchangeably be dissolved in and contain peroxide organic acid, hydrogen peroxide and free protons H +solvent in, to promote the dissolubility of chitose, and then make of the present inventionly to reach splendid film forming except bacteria composition, to act on hard or soft object.
On the other hand, because the bacteria composition (being chitose and peroxide organic acid composition) that removes of the present invention has oxidability, if so while acting on the metal object of easy oxidation, the present invention can further add corrosion resistant component, to avoid causing metal object surface corrosion under Long Term Contact, in other words, not disturb bacteria-eliminating efficacy under front topic, the present invention can add the compound that possesses anticorrosive function, through compatible and effective dose test, be added in chitose and peroxide organic acid composition, for instance, the content that the present invention adds corrosion resistant component can be 10ppm-10%(w/w), and this corrosion resistant component can be selected from nitric acid, nitrate, phosphoric acid, phosphate, hypophosphorous acid, hypophosphites, pyrophosphoric acid, pyrophosphate, alkali metal, divalence and trivalent metal ion form one or more compositions in group, more specifically, described corrosion resistant component can be in order to adjust peroxide organic acid corrosivity.
Again on the one hand; when of the present invention while being applied in the degerming of object and conservation treatment except bacteria composition (being chitose and peroxide organic acid composition); also can add interfacial agent; to improve wettability, permeability, foaming characteristic, stability of liquid etc.; to promote its degerming protection effect; for instance; the content that adds interfacial agent as the present invention is 0.1-20%(w/w) time; not only can improve the advantage such as covering property and tackness of diaphragm for object in order to improve liquid dissolubility and stability, to have more.In addition, the content that adds interfacial agent as the present invention is 1-99%(w/w) time, the present invention can be made into possess clean, degerming, the multi-functional product such as protection, such as cleaning agent etc.
In sum; for peroxide organic acid and chitose, the two forms stable except bacteria composition in the present invention; because peroxide organic acid and chitose are the not safe antibacterial agent of residual toxicity; and peroxide organic acid possesses the quick-acting sterilizing power of wide spectrum; and chitose possesses the feature of long acting antibiotic and excellent film forming; so peroxide organic acid and chitose have complementary functions, be applicable to composition safety, convenient, efficient degerming protectant.In addition, of the present invention except bacteria composition, its peroxide organic acid and chitose are with compatibility, stability and the splendid advantage such as stability and degerming synergistic effect of height.
Refer to shown in Fig. 1, the present invention comprises the following steps except bacteria composition acts on body surface execution degermation: process step S101, process step S102 and process step S103.
Please arrange in pairs or groups with reference to figure 2A to Fig. 2 C, Fig. 2 A is the degerming schematic diagram () that the present invention removes bacteria composition and act on body surface, Fig. 2 B is the degerming schematic diagram (two) that the present invention removes bacteria composition and act on body surface, and Fig. 2 C is for the present invention acts on the degerming schematic diagram (three) of body surface except bacteria composition; Need special instruction, the embodiment that Fig. 2 A to Fig. 2 C is Optimal State.As shown in Figure 2 A, the present invention is at the beginning by chitose (Ch-NH 2) and peroxide organic acid (RCOOOH) form there is homogeneous chitose cation (Ch-NH except bacteria composition 3 +) and peroxy (RCOOO -) ion pair (process step S101).Then, as shown in Figure 2 B, the bacteria composition that removes of the present invention utilizes chitose cation (Ch-NH at body surface 3 +) and peroxy (RCOOO -) discharge free oxygen base ([O]) carry out degermation (process step S102).Afterwards, as shown in Figure 2 C, of the present invention except bacteria composition is at body surface formation degerming diaphragm, peroxy (RCOOO -) discharge acid radical anion (RCOO -) and chitose cation (Ch-NH 3 +) formation chitose salt (Ch-NH 3 +... -oOCR), to carry out long-acting degerming (process step S103).
Can spread to the relevant content of implementing according to this for more understanding the disclosed content of specification of the present invention, now illustrate as follows with datumization: about the Application Example except bacteria composition of the present invention, chitose (chitosan, CH) take from the same batch of product taking crab shell as raw material: weight molecular weight Mw=100,000-150,000, Deacetylation degree DD=86%, Peracetic acid (peroxyaceticacid, PAA) is purchased from the reagent of Sigma Aldrich.Peroxycaprylic acid (peroxyoctanoic acid, POA) adopts the commodity Octave of Ecolab Inc. tM.
embodiment 1
In the beaker of 1L, first put into the Peracetic acid of 50g, thin up, the gross weight of Peracetic acid and water reaches 950g, then adds 50g chitose, and this mixture (containing 5%CH, 1.6%PAA) continues to stir after 5 hours at normal temperatures, stop stirring, leave standstill 24 hours at room temperature.In the beaker of 1L, first put into the Octave of 50g tMthin up, Octave tMreach 925g with the gross weight of water, add 50g chitose, then add 25g glacial acetic acid (AA), this mixture (containing 5%CH, 4.7%POA, 2.5%AA) is settled at normal temperatures, continues to stir after 5 hours, stops stirring, leave standstill 24 hours at room temperature, obtain the uniform aqueous solution.In addition, preparation control sample (containing 5%CH, 2.5%AA): in 925g water, add 25g glacial acetic acid and 50g chitose, stir at normal temperatures after 5 hours, stop stirring, leave standstill 24 hours at room temperature, obtain the uniform aqueous solution.Measure viscosity and carry out antibacterial tests for these 5 liquor samples.The viscosity test of above-listed sample is used Broofield Rotary Viscosimeter 20 DEG C of measurements, as shown in the table:
Sample Chitose Peroxide organic acid Acetic acid Viscosity
A 5% - 2.5% 32000cp
B 5% 5%PAA - 600cp
C 5% 5%Octave 2.5% 850cp
With minimal inhibitory concentration (Minimum Inhibitory Concentration Test, MIC) test method(s) carries out antibacterial effect detection, for Escherichia coli Escherichia coli and melanomyces Aspergillus niger checking antibacterial effect, each sample is diluted to 6 samples with nutrient solution respectively, and its chitose concentration is respectively 25,50,75,100,250,500ppm.The sample of dilution is divided into two groups, implants respectively Escherichia coli and melanomyces.Colibacillary sample is cultivated after 24 hours at 37 DEG C, inspects Bacteria cold shock; The sample of melanomyces is cultivated after 15 days at 28 DEG C, inspects fungus growth state, and experimental data is as shown in the table:
(+): growth microorganism, (-): not long microorganism
embodiment 2
Preparation, containing chitose/peroxide organic acid composition thing of stabilization agent and corrosion inhibitor, is verified bactericidal effect (bactericidal disinfectant efficacy).Prepare sample D: in the beaker of 1L, first put into 500g water, sequentially add 10g chitose, 10g Peracetic acid (C 2h 4o 3, peroxyaceticacid, PAA), 2g hypophosphorous acid (RPO(OH) 2, phosphonic acid), 5g dikalium phosphate (K 2hPO 4, dipotassium hydrogen orthophosphate), then add water and make solution gross weight reach 1000g.Continue at normal temperatures to stir after 5 hours, stop stirring, leave standstill 24 hours at room temperature, obtain uniform liquid.The composition of sample E: 10g chitose, 5g Peroxycaprylic acid Octave tM, 5g glacial acetic acid, 3g hypophosphorous acid, 7g dikalium phosphate, 970g water, preparation method is as sample D.
The bactericidal assay of sample D and sample E is used AOAC960.09 test method(s) (Germicidaland Detergent Sanitizing Action of Disinfectants), tests for staphylococcus aureus (Staphylococcus aureus) and Escherichia coli (Escherichia coli) two strain bacterium.Sample, with 100 times of AOAC standard hard water dilutions (containing 100ppm chitose), is got 99ml dilution sample and is planted bacterium 1ml contact 30sec, 60sec at 20 DEG C, carries out bacterium colony recovery, cultivation, counting.Diluted sample plant bacterium number (by blank solution reclaim counting): staphylococcus aureus=5.8x10 7cFU, Escherichia coli=6.3x10 7cFU
Express result of the test with degerming rate (Log Reduction) as shown in the table:
CFU:colonyforming?units.
In sum, the bacteria composition that removes that the embodiment of the present invention provides has following useful effect:
Of the present invention except bacteria composition; its peroxide organic acid and chitose are the not safe antibacterial agent of residual toxicity; peroxide organic acid possesses the quick-acting sterilizing power of wide spectrum; chitose possesses the feature of long acting antibiotic and excellent film forming; the two has complementary functions, and is applicable to composition safety, convenient, efficient degerming protectant.
Of the present invention except bacteria composition, its peroxide organic acid and chitose have compatibility, stability and the splendid advantage such as stability and degerming synergistic effect of height.
Of the present invention except bacteria composition, its peroxide organic acid and chitose can disposablely complete, and do not need to mix other antibacterial compositions and can form stable except bacteria composition, so have the advantages such as saving Production Time and cost.
Of the present invention except bacteria composition, it can concentration on demand be made into the aqueous solution, acts on hard or soft object, reach sterilization, fungi-proofing, except spot function, and can form degerming diaphragm at body surface.
But the foregoing is only preferred embodiment of the present invention, be non-ly intended to limit to protection scope of the present invention, so the equivalence that all utilizations specification of the present invention and accompanying drawing content are done changes, be all in like manner all contained in protection scope of the present invention explanation hereby.

Claims (13)

1. one kind is removed bacterium Group compound, comprise chitose and peroxide organic acid, it is characterized in that, should be except the two composition ratio of chitose of bacteria composition and peroxide organic acid: chitose/peroxide organic acid (w/w)=0.01-100, chitose is selected from amido monose, amide groups monose, chitosan oligosaccharide, shitosan and forms one or more compositions in group, peroxide organic acid is the aliphatic peroxide organic acid of C1-C18, and it is selected from the acid of peroxide list carbonyl, two carbonyl one or more compositions in group that acid forms of peroxide;
Wherein, should can be diluted to the bacteria remover that contains >10ppm chitose except bacteria composition.
2. as claimed in claim 1 except bacteria composition, it is characterized in that: should further comprise 0.01-30%(w/w except bacteria composition) hydrogen peroxide.
3. as claimed in claim 1 except bacteria composition, it is characterized in that: should further comprise 0.01-30%(w/w except bacteria composition) the organic acid of C1-C18.
4. as claimed in claim 3 except bacteria composition, it is characterized in that: this organic acid is selected from formic acid, acetic acid, propionic acid, sad, capric acid, lactic acid, glutaric acid, adipic acid, decanedioic acid, citric acid, malic acid, maleic acid, gluconic acid, amino acid forms one or more compositions in group.
5. as claimed in claim 1 except bacteria composition, it is characterized in that: should further comprise 0.01-30%(w/w except bacteria composition) hydrogen peroxide and 0.01-30%(w/w) the organic acid of C1-C18.
6. as claimed in claim 5 except bacteria composition, it is characterized in that: this organic acid is selected from formic acid, acetic acid, propionic acid, sad, capric acid, lactic acid, glutaric acid, adipic acid, decanedioic acid, citric acid, malic acid, maleic acid, gluconic acid, amino acid forms one or more compositions in group.
7. as claimed in claim 1 except bacteria composition, it is characterized in that: should further comprise a stabilization agent that makes peroxide stable except bacteria composition, this stabilization agent content is 10ppm-10%(w/w).
8. as claimed in claim 7 except bacteria composition, it is characterized in that: this stabilization agent is selected from stannate, pyrophosphate, silicate, sulphate, sulfonate and forms one or more compositions in group.
9. as claimed in claim 1 except bacteria composition, it is characterized in that: should further comprise corrosion resistant component except bacteria composition, its content is 10ppm-10%(w/w).
10. as claimed in claim 9 except bacteria composition, it is characterized in that: this corrosion resistant component is selected from nitric acid, nitrate, phosphoric acid, phosphate, hypophosphorous acid, hypophosphites, pyrophosphoric acid, pyrophosphate, alkali metal, divalence and trivalent metal ion and forms one or more compositions in group.
11. is as claimed in claim 1 except bacteria composition, it is characterized in that: should further comprise interfacial agent except bacteria composition, the content of its interfacial agent is 0.1-20%(w/w).
12. is as claimed in claim 1 except bacteria composition, it is characterized in that: should further comprise interfacial agent except bacteria composition, the content of its interfacial agent is 1-99%(w/w).
13. as described in any one in claim 1 to 12 except the application of bacteria composition, it is characterized in that: should keep out the injury of microorganism for the protection of object except bacteria composition.
CN201210554611.1A 2012-12-19 2012-12-19 Degerming composition and application thereof Pending CN103875666A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107441494A (en) * 2017-07-28 2017-12-08 中国科学院过程工程研究所 A kind of chitosan oligosaccharide of antibacterial film activity and antibiotic and its application
CN111713499A (en) * 2020-06-04 2020-09-29 西安超视距智能科技有限公司 Synthesis method of biological sugar multi-ionic liquid

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1680452A (en) * 2004-06-25 2005-10-12 中国科学院海洋研究所 Preparation of chitin oligose compound
CN102613179A (en) * 2012-03-12 2012-08-01 河北科技大学 Sterilizing agent for preventing and curing diseases of vegetables and fruits and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1680452A (en) * 2004-06-25 2005-10-12 中国科学院海洋研究所 Preparation of chitin oligose compound
CN102613179A (en) * 2012-03-12 2012-08-01 河北科技大学 Sterilizing agent for preventing and curing diseases of vegetables and fruits and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107441494A (en) * 2017-07-28 2017-12-08 中国科学院过程工程研究所 A kind of chitosan oligosaccharide of antibacterial film activity and antibiotic and its application
CN111713499A (en) * 2020-06-04 2020-09-29 西安超视距智能科技有限公司 Synthesis method of biological sugar multi-ionic liquid

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