JP2001211742A - Culture medium for cultivating pleurotus eryngii - Google Patents

Abstract

PROBLEM TO BE SOLVED: To reutilize a used culture medium daily rejected in a large amount in the harvesting period as a culture medium for artificially cultivating Pleurotus eryngii and more increase the yield of the Pleurotus eryngii. SOLUTION: This culture medium for cultivating the Pleurotus eryngii is obtained by pulverizing a used culture medium after carrying out the cultivation of mushrooms by adding required nutriments and water to a culture medium substrate, providing a used culture medium, mixing the resultant used culture medium with an unused culture medium substrate such as a large sawdust or a corncob and mixing the resultant mixture with required nutriments and other additives.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a culture medium for cultivation of eryngii, wherein a used culture medium after mushroom cultivation in a bottle is partially used as a culture medium for cultivation of eryngii.

[0002]

2. Description of the Related Art In general, cultivation of eryngii has a short history unlike bottle cultivation of common edible mushrooms such as enokitake mushrooms and bunashimeji, and the cultivation test started from around 1993. Is still in the developing stage.

[0003] In 1995, a method for producing and cultivating a new strain was developed (Japanese Patent No. 2881293), and cultivation of eryngii has been stabilized only recently.

[0004]

However, in mushroom cultivation, it is an issue for producers to improve productivity, that is, to increase production at low cost. Therefore, it is of the utmost importance for a producer who is cultivating bottles to increase the yield from one bottle and to reduce the cost per bottle.

On the other hand, used mushroom cultivation medium is scraped out of bottles and composted and provided for agriculture. In recent years, organic matter discharged from various factories has been composted and provided. However, organic fertilizers for agriculture have also tended to be oversupplied, and if the entire amount of spent medium discharged daily is composted on top of that, there has been the problem that organic fertilizers will be excessive in some areas.

[0006] For this reason, at present, a large amount of used medium is treated as industrial waste, and at present, corn cob resources that must be imported are wasted.

In view of such a situation, the method for preparing an eryngii medium using a used medium according to the present invention uses a used medium that is generated in large quantities every day at the time of harvesting for the artificial cultivation of eryngii, which has recently been industrialized. Reused as a medium for
The object of the present invention is to stabilize the supply of a medium substrate by reducing the amount of corn cob used, and to reduce the cost per bottle to improve productivity.

[0008]

The feature of the eryngii cultivation medium according to the present invention for solving the above-mentioned conventional problems and achieving the intended object is that nutrients and water necessary for the culture medium base are provided. In addition, the used medium obtained by pulverizing the used medium after mushroom cultivation is mixed with the unused medium base material such as sawdust or corn cob, and the necessary nutrient elements and other additives are mixed. In the medium.

The ratio of the unused medium base material to the used medium is 7: 3 to 3: 7 (weight ratio), and the used medium medium is corn cob. Is preferred.

In cultivation of eryngii, increasing the yield per bottle is the most effective way to increase the yield using existing equipment as it is.

On the other hand, to increase the yield per bottle, a. Develop nutrients that increase sales. b. Increase the amount of nutrients per bottle. c. Increase the volume of medium.
However, there are no inexpensive and effective nutrients that have the effect of increasing the yield. If the amount of nutrient per bottle is increased, the cost per unit medium increases, and the amount of nutrient per bottle increases. When the amount of the medium is increased more than the current level, the degree of consolidation increases, the medium becomes hard and the culture is not smooth, and the elongation of the hypha stops halfway, resulting in poor culture.

Therefore, the present inventors have found that the used medium conventionally discarded contains a large amount of nutrients that have not been sucked up in the previous mushroom cultivation and substances that remain in the mycelium and are effective for mushroom cultivation. Focusing on the fact that it is inexpensive, by using it, it is possible to provide an inexpensive and nutritious medium, increase the yield per bottle, and develop the eryngii cultivation medium of the present invention that can improve productivity. did.

[0013]

BEST MODE FOR CARRYING OUT THE INVENTION Preparation of Used Medium Used for artificial cultivation using mushroom cultivation bottles such as Bunashimeji and Elingi, and scraping off the used medium remaining in the cultivation bottle after mushroom harvesting by a scraping device. The used medium in a coarsely crushed state obtained by the above is dried.

For this drying, a dryer 1 shown in FIG. 1 is used. This dryer accommodates a large number of horizontal conveyors 3, 3... Each using a breathable mesh-shaped conveyor belt in a multi-stage arrangement in a drying chamber 2, and on each horizontal conveyor,
While the coarsely crushed spent medium is sequentially moved horizontally and sequentially dropped from the end portion 3b of the upper horizontal conveyor to the start end 3a of the lower horizontal conveyor, and conveyed, the lower blowing port 4 of the drying chamber 2 is provided. , 4 to be dried to a moisture content of 7 to 35%.

The hot air sent from the air outlet 4 is cooled in the drying chamber 2, discharged from the upper exhaust port 5, and discharged through the dust collector to the atmosphere.

During the drying, a used and crushed and dried medium in which granular materials of about 6 to 60 mesh are mixed is used, and this is used to make the particle size distribution and the distribution of the mushrooms of the pre-cultivated mushroom contained in the medium uniform. Stirring.

When long-term storage is required, the moisture content is set to about 7 to 12%, and when it is immediately reused, it is dried to about 12 to 35%.

Preparation of Cultivation Medium A used medium crushed and dried as described above and an unused crushed corn cob are mixed to form a main raw material, and rice bran, a selected tube bran and other necessary nutrients are added thereto. , Sorghum,
And supplementary ingredients (additives) such as soybean hull, cotton hull, coconut shell, peat moss, etc.
Water is added thereto to adjust the water content to a water content of about 58 to 68% by weight (preferably 63%).

This is packed in a cultivation bottle for cultivation of eryngii, subjected to necessary sterilization treatment, inoculated with a seed fungus, and cultivated eryngii.

Test Example Corncob, soybean hulls, and rice bran were mixed at a weight ratio of 10: 5: 6 as target areas, and tap water was added thereto so that the water content became 63% by weight, followed by stirring to adjust the culture medium. This has a capacity of 650
Filled in a wide-mouthed cultivation container of cm3 and sterilized at 120 ° C. When the temperature of the culture medium became 20 ° C or lower, inoculated with eryngii inoculum, capped and cultured for 35 days in a room adjusted to room temperature of 20 ° C and 60% humidity did.

Next, the cap was removed, and 1 cm of bacteria was scraped from above the culture medium. This was cultured at a temperature of 17 ° C. and a relative humidity of 70 to 80% to form fruiting bodies.

As a test plot 1, 30% of the corn cob in the target plot was replaced with a used medium to adjust the culture medium.
The cells were cultured in the same manner as in the control.

As a test plot 2, the culture medium was adjusted by replacing 50% of the corn cob in the target plot with a used medium.
The cells were cultured in the same manner as in the control.

As a test plot 3, the culture medium was adjusted by replacing 70% of the corn cob in the target plot with a used medium.
The cells were cultured in the same manner as in the control.

In test plot 4, the culture medium was adjusted by replacing the whole amount of corn cob in the target plot with a used medium, and cultured in the same manner as in the target plot.

The results are as shown in Table 1.

Table 1

As shown in the results of Table 1, when eryngii is cultivated in a culture medium in which 30 to 70% of the corn cob in the target area has been replaced with a used medium, when cultivated in a culture medium adjusted without using a used medium. As compared with the method, the number of days required to obtain fruiting bodies was shortened, and a higher yield was obtained.

[Brief description of the drawings]

FIG. 1 is a cross-sectional view schematically showing an example of an apparatus for drying a fresh medium used in the present invention.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Dryer 2 Drying room 3 Horizontal conveyor 3a Starting end 3b Ending end 4 Blow port 5 Exhaust port

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[Procedure amendment]

[Submission Date] February 18, 2000 (2000.2.1
8)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] Brief description of drawings

[Correction method] Deleted

[Procedure amendment]

[Submission date] February 28, 2000 (200.2.2
8)

[Procedure amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0007

[Correction method] Change

[Correction contents]

In view of such a situation, the method for preparing an eryngii medium using a used medium according to the present invention is a method for preparing a large amount of used medium every day during harvesting for artificial cultivation of eryngii which has recently been industrialized. Reused as a medium for
The object of the present invention is to stabilize the supply of a medium substrate by reducing the amount of corn cob used, and to reduce the cost per bottle to improve productivity.

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0014

[Correction method] Change

[Correction contents]

For this drying, a dryer is used. This dryer accommodates a large number of horizontal conveyors, each using a breathable mesh-like conveyor belt, in a multi-stage arrangement in a drying chamber, and sequentially moves coarsely-crushed spent medium horizontally on each horizontal conveyor. While being successively dropped from the end of the horizontal conveyor to the start of the lower horizontal conveyor, and then conveyed, it is dried to a moisture content of 7 to 35% by exposing it to hot air sent from an air blowing port at the lower part of the drying chamber. Let it.

[Procedure amendment 3]

[Document name to be amended] Statement

[Correction target item name] 0015

[Correction method] Change

[Correction contents]

The hot air sent from the air outlet is cooled in the drying chamber, discharged from the upper exhaust port, and discharged to the atmosphere through the dust collector.

 ────────────────────────────────────────────────── ─── Continuing on the front page (72) Inventor Satoshi Inatomi 800-8 Shimokomazawa, Daino, Nagano City, Nagano Prefecture Inside the Kinoko Research Laboratory, Hokuto Sangyo Co., Ltd. (72) Inventor Masayuki Mizuno 138-1 Murahito, Minamijiji, Nagano City, Nagano Prefecture F-term in Hokuto Sangyo Co., Ltd. (reference) 2B011 AA07 BA06 BA07 BA13 GA04

Claims (3)

[Claims] 1. A used culture medium obtained by pulverizing a used culture medium after cultivating mushrooms by adding necessary nutrients and water to the culture medium base, and an unused culture medium base such as sawdust or corn cob. A medium for cultivation of eryngii, which is mixed and mixed with necessary nutrients and other additives. 2. The eryngii cultivation medium according to claim 1, wherein the ratio of the unused medium base material to the used medium is 7: 3 to 3: 7 (weight ratio). 3. The eryngii cultivation medium according to claim 1, wherein the used culture medium is corn cob.