JP2000509994A - 細胞培養培地への金属依存性プロテアーゼ又はキモトリプシンの阻害物質の添加を含む組換えポリペプチドの製造法 - Google Patents
細胞培養培地への金属依存性プロテアーゼ又はキモトリプシンの阻害物質の添加を含む組換えポリペプチドの製造法Info
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- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Toxicology (AREA)
- Plant Pathology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Diabetes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9601855A SE9601855D0 (sv) | 1996-05-14 | 1996-05-14 | Process for producing a protein |
| US1887496P | 1996-05-29 | 1996-05-29 | |
| US9601855-1 | 1996-05-29 | ||
| US60/018,874 | 1996-05-29 | ||
| PCT/SE1997/000783 WO1997043436A1 (en) | 1996-05-14 | 1997-05-13 | A process for producing a recombinant polypeptide involving the addition of an inhibitor of metal-dependent proteases or chymotrypsins to the cell culture medium |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009001597A Division JP4896999B2 (ja) | 1996-05-14 | 2009-01-07 | 細胞培養培地への金属依存性プロテアーゼ又はキモトリプシンの阻害物質の添加を含む組換えポリペプチドの製造法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2000509994A true JP2000509994A (ja) | 2000-08-08 |
| JP2000509994A5 JP2000509994A5 (https=) | 2004-12-09 |
Family
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Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP09540800A Pending JP2000509994A (ja) | 1996-05-14 | 1997-05-13 | 細胞培養培地への金属依存性プロテアーゼ又はキモトリプシンの阻害物質の添加を含む組換えポリペプチドの製造法 |
| JP2009001597A Expired - Fee Related JP4896999B2 (ja) | 1996-05-14 | 2009-01-07 | 細胞培養培地への金属依存性プロテアーゼ又はキモトリプシンの阻害物質の添加を含む組換えポリペプチドの製造法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009001597A Expired - Fee Related JP4896999B2 (ja) | 1996-05-14 | 2009-01-07 | 細胞培養培地への金属依存性プロテアーゼ又はキモトリプシンの阻害物質の添加を含む組換えポリペプチドの製造法 |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5851800A (https=) |
| EP (1) | EP0934424B1 (https=) |
| JP (2) | JP2000509994A (https=) |
| AT (2) | ATE321141T1 (https=) |
| AU (1) | AU716245B2 (https=) |
| CA (1) | CA2253287C (https=) |
| DE (2) | DE69735510T2 (https=) |
| DK (1) | DK0934424T3 (https=) |
| ES (2) | ES2270460T3 (https=) |
| NO (2) | NO325358B1 (https=) |
| NZ (1) | NZ332751A (https=) |
| PT (2) | PT1318198E (https=) |
| WO (1) | WO1997043436A1 (https=) |
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| US6475725B1 (en) * | 1997-06-20 | 2002-11-05 | Baxter Aktiengesellschaft | Recombinant cell clones having increased stability and methods of making and using the same |
| EP2921180B1 (en) | 1999-02-22 | 2019-08-14 | University of Connecticut | Albumin-free factor VIII formulations |
| AT409379B (de) | 1999-06-02 | 2002-07-25 | Baxter Ag | Medium zur protein- und serumfreien kultivierung von zellen |
| ATE330003T1 (de) * | 1999-08-05 | 2006-07-15 | Baxter Ag | Rekombinanter stabiler zellklon, seine herstellung und verwendung |
| WO2001052891A1 (fr) * | 2000-01-20 | 2001-07-26 | Nippon Organon K. K. | Inhibiteurs d'invasion plasmodiale dans des erythrocytes |
| PT2287288E (pt) * | 2002-07-09 | 2012-12-10 | Baxter Int | Meio isento de proteína animal para cultura de células |
| CN101857851A (zh) * | 2002-12-23 | 2010-10-13 | 布里斯托尔-迈尔斯斯奎布公司 | 用于蛋白质生产的哺乳动物细胞培养方法 |
| DE60335024D1 (de) * | 2002-12-23 | 2010-12-30 | Bristol Myers Squibb Co | Produktqualitätsverbesserung in säugerzellkulturverfahrenzur proteinproduktion |
| US20040265964A1 (en) * | 2003-04-25 | 2004-12-30 | Martin Allen | Inducers of recombinant protein expression |
| US7255288B2 (en) * | 2004-03-08 | 2007-08-14 | Wan Shan Chan | Aroma therapy for fountain |
| US20060094104A1 (en) * | 2004-10-29 | 2006-05-04 | Leopold Grillberger | Animal protein-free media for cultivation of cells |
| EP1707634A1 (en) * | 2005-03-29 | 2006-10-04 | Octapharma AG | Method for isolation of recombinantly produced proteins |
| EP3653699A1 (en) | 2006-01-04 | 2020-05-20 | Baxalta Incorporated | Oligopeptide-free cell culture media |
| LT2126106T (lt) | 2007-02-23 | 2018-01-10 | Sk Chemicals Co., Ltd. | Viii faktoriaus ir jo darinių gavimo ir gryninimo būdas |
| EP1988101A1 (en) | 2007-05-04 | 2008-11-05 | Novo Nordisk A/S | Improvement of factor VIII polypeptide titers in cell cultures |
| WO2008135498A2 (en) * | 2007-05-04 | 2008-11-13 | Novo Nordisk A/S | Prevention of protein degradation in mammalian cell cultures |
| NZ593190A (en) | 2008-11-07 | 2013-01-25 | Baxter Int | Factor viii formulations |
| US9540426B2 (en) | 2009-10-06 | 2017-01-10 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
| CN107190034A (zh) | 2010-10-05 | 2017-09-22 | 诺沃—诺迪斯克保健股份有限公司 | 生产蛋白质的方法 |
| EP2906683B1 (en) | 2012-10-15 | 2017-05-31 | Bristol-Myers Squibb Company | Mammalian cell culture processes for protein production |
| KR102668200B1 (ko) | 2015-10-28 | 2024-05-23 | 주식회사유한양행 | 지속형 fgf21 융합 단백질 및 이를 포함하는 약학적 조성물 |
| KR102670157B1 (ko) | 2015-10-28 | 2024-05-29 | 주식회사유한양행 | 이중 작용 단백질 및 이를 포함하는 약학적 조성물 |
| AU2017358289C1 (en) | 2016-11-10 | 2025-05-08 | Yuhan Corporation | Pharmaceutical composition for preventing or treating hepatitis, hepatic fibrosis, and hepatic cirrhosis comprising fusion proteins |
| CN110709520B (zh) | 2017-04-21 | 2024-04-05 | 株式会社柳韩洋行 | 用于产生双功能蛋白质及其衍生物的方法 |
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| US5149787A (en) * | 1984-05-22 | 1992-09-22 | The Blood Center Research Foundation | Method for maintaining intact, non-degraded factor VIII/von-Willebrand factor during blood processing |
| SE8501050D0 (sv) * | 1985-03-05 | 1985-03-05 | Kabivitrum Ab | Biologically active fragments of human antihemophilic factor and method for preparation thereof |
| US5279939A (en) * | 1986-08-18 | 1994-01-18 | Smithkline Beecham Corporation | Protein protease inhibitors from streptomyces |
| US4891356A (en) * | 1987-07-15 | 1990-01-02 | Brigham & Women's Hospital | Proteinase inhibitors for treatment of gastrointestinal ulcer disease |
| JPH0387173A (ja) * | 1987-09-10 | 1991-04-11 | Teijin Ltd | ヒト活性化天然型ファクター８ｃの製造方法及びそれに用いる形質転換体 |
| CA1340740C (en) * | 1987-12-08 | 1999-09-14 | Eileen R. Mulvihill | Co-expression in eukaryotic cells |
| JP2769170B2 (ja) * | 1987-12-08 | 1998-06-25 | ザイモジェネティクス，インコーポレイティド | 真核細肪中での同時発現 |
| DK457788D0 (da) * | 1988-08-16 | 1988-08-16 | Nordisk Gentofte | Fremgangsmaade til fremstilling af et oensket polypeptid |
| WO1990002175A1 (en) * | 1988-08-16 | 1990-03-08 | Novo Nordisk A/S | A method of producing polypeptides by culturing eukaryotic cells in the presence of protease inhibitors |
| GB8827305D0 (en) * | 1988-11-23 | 1988-12-29 | British Bio Technology | Compounds |
| DK105489D0 (da) * | 1989-03-03 | 1989-03-03 | Novo Nordisk As | Polypeptid |
| SE465222C5 (sv) * | 1989-12-15 | 1998-02-10 | Pharmacia & Upjohn Ab | Ett rekombinant, humant faktor VIII-derivat och förfarande för dess framställning |
| DE69133064T2 (de) * | 1990-02-26 | 2003-03-06 | The Board Of Trustees Of The Leland Stanford Junior University, Stanford | Identifizierung und expression von insekten-steroidrezeptor-dns-sequenzen |
| US5189178A (en) * | 1990-11-21 | 1993-02-23 | Galardy Richard E | Matrix metalloprotease inhibitors |
| US5304603A (en) * | 1991-06-18 | 1994-04-19 | The Population Council | Leydig cell stimulator |
| US5661034A (en) * | 1991-07-18 | 1997-08-26 | Fuji Yakuhin Kogyo Kabushiki Kaisha | Serum-free medium for tissue culture containing tissue inhibitor of metalloproteinases and method for growing cells using the medium |
| WO1993006217A1 (en) * | 1991-09-19 | 1993-04-01 | Genentech, Inc. | EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES |
| CA2078721A1 (en) * | 1991-09-24 | 1993-03-25 | Hiroshi Yonemura | Process for preparing human coagulation factor viii protein complex |
| US5278289A (en) * | 1991-11-12 | 1994-01-11 | Johnson Alan J | Antihemophilic factor stabilization |
| US5296471A (en) * | 1992-12-22 | 1994-03-22 | Glycomed Incorporated | Method for controlling o-desulfation of heparin and compositions produced thereby |
| WO1995005477A1 (en) * | 1993-08-12 | 1995-02-23 | University Of Maryland | Thermostable alkaline metalloprotease produced by a hyphomonas, and preparation thereof |
| US5631159A (en) * | 1993-09-22 | 1997-05-20 | Creative Biomolecules, Inc. | Lipid-modified serum free media |
| US5543396A (en) * | 1994-04-28 | 1996-08-06 | Georgia Tech Research Corp. | Proline phosphonate derivatives |
-
1997
- 1997-05-07 US US08/852,783 patent/US5851800A/en not_active Expired - Lifetime
- 1997-05-13 JP JP09540800A patent/JP2000509994A/ja active Pending
- 1997-05-13 DE DE69735510T patent/DE69735510T2/de not_active Expired - Lifetime
- 1997-05-13 WO PCT/SE1997/000783 patent/WO1997043436A1/en not_active Ceased
- 1997-05-13 AT AT03000391T patent/ATE321141T1/de active
- 1997-05-13 NZ NZ332751A patent/NZ332751A/en not_active IP Right Cessation
- 1997-05-13 AT AT97923382T patent/ATE334223T1/de active
- 1997-05-13 AU AU29197/97A patent/AU716245B2/en not_active Ceased
- 1997-05-13 PT PT03000391T patent/PT1318198E/pt unknown
- 1997-05-13 DK DK97923382T patent/DK0934424T3/da active
- 1997-05-13 EP EP97923382A patent/EP0934424B1/en not_active Expired - Lifetime
- 1997-05-13 PT PT97923382T patent/PT934424E/pt unknown
- 1997-05-13 CA CA002253287A patent/CA2253287C/en not_active Expired - Fee Related
- 1997-05-13 DE DE69736391T patent/DE69736391T2/de not_active Expired - Lifetime
- 1997-05-13 ES ES97923382T patent/ES2270460T3/es not_active Expired - Lifetime
- 1997-05-13 ES ES03000391T patent/ES2260524T3/es not_active Expired - Lifetime
-
1998
- 1998-11-13 NO NO19985297A patent/NO325358B1/no not_active IP Right Cessation
-
2006
- 2006-11-02 NO NO20065027A patent/NO325633B1/no not_active IP Right Cessation
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2009
- 2009-01-07 JP JP2009001597A patent/JP4896999B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2253287A1 (en) | 1997-11-20 |
| WO1997043436A1 (en) | 1997-11-20 |
| JP4896999B2 (ja) | 2012-03-14 |
| EP0934424B1 (en) | 2006-07-26 |
| DE69736391T2 (de) | 2007-08-16 |
| DE69735510T2 (de) | 2006-08-24 |
| AU716245B2 (en) | 2000-02-24 |
| CA2253287C (en) | 2008-02-19 |
| NO20065027L (no) | 1999-01-14 |
| EP0934424A1 (en) | 1999-08-11 |
| PT934424E (pt) | 2006-12-29 |
| US5851800A (en) | 1998-12-22 |
| DE69735510D1 (de) | 2006-05-11 |
| DK0934424T3 (da) | 2006-11-20 |
| ATE321141T1 (de) | 2006-04-15 |
| NO985297L (no) | 1999-01-14 |
| NO325633B1 (no) | 2008-06-30 |
| ATE334223T1 (de) | 2006-08-15 |
| AU2919797A (en) | 1997-12-05 |
| DE69736391D1 (de) | 2006-09-07 |
| NO985297D0 (no) | 1998-11-13 |
| ES2260524T3 (es) | 2006-11-01 |
| PT1318198E (pt) | 2006-08-31 |
| NO325358B1 (no) | 2008-04-07 |
| JP2009148271A (ja) | 2009-07-09 |
| ES2270460T3 (es) | 2007-04-01 |
| NZ332751A (en) | 2000-06-23 |
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