JP2000270851A - Microorganism material and its use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a microorganism material having a high plant disease- inhibiting effect and plant growth-accelerating effect and useful for agriculture or horticulture by mixing a specific fermented material and microorganisms belonging to the genus Phoma. SOLUTION: This microorganism material is obtained by mixing a fermented material obtained by fermenting organic materials such as rice bran, corn bran, etc., at >=40 deg.C, preferably >=65 deg.C temperature for >=2 days, with a material containing one or more kinds of microorganisms belonging to the genus Phoma selected from the group consisting of Phoma sp GS-8-2 (FERM P-13003), Phoma sp-GS-10-2 (FERM-P-13129) and Phoma sp GS-12-2 (FERM P-13004). Further, it is preferable to produce a plant growing soil by including the microorganism material.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to a microbial material and its use.

[0002]

2. Description of the Related Art Conventionally, various microorganism materials have been used for the purpose of suppressing plant diseases and promoting plant growth.

[0003]

However, the effects of these microbial materials on controlling plant diseases and promoting plant growth have not always been satisfactory.

[0004]

Means for Solving the Problems In view of the above situation, the present inventors have made intensive studies to find a better microorganism material. As a result, the organic material was ripened at a temperature of 40 ° C. or more for 2 days or more. It has been found that a microbial material obtained by mixing a rotten product and a material containing a microorganism of the genus Phoma exhibits a high plant disease suppressing effect and a plant growth promoting effect. That is, the present invention provides a microbial material obtained by mixing a rotten product obtained by ripening an organic material at a temperature of 40 ° C. or more for 2 days or more and a material containing a microorganism of the genus Phoma (hereinafter, referred to as the microbial material of the present invention). And its uses.

[0005]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described in detail. First, a method for producing a persimmon used in producing the microorganism material of the present invention will be described. Production Method 1 A decay product can be produced by depositing an organic material and ripening it with microorganisms floating in the air or adhering to the organic material. On this occasion,
The organic material is usually heated to 40 ° C. or higher by appropriately adjusting the amount and height of the organic material so that the temperature rise due to the heat of ripening generated during ripening is greater than the temperature decrease due to the heat radiation, and sufficient oxygen can be supplied. , Preferably 6
It can be ripened at a temperature of 5 ° C. or more for 2 days or more.
Organic materials used in such a production method include:
It is not particularly limited as long as it can be ripened, for example,
Rice, barley, wheat, corn, soybean, kouryan,
Cassava, rice straw, straw, chaff, bark, sawdust,
Rice bran, corn bran, bran, barley bran, peanut grounds, sunflower grounds, corn germ, pruning waste, pasture, wild grass and other plants and their residues, mushroom medium residues, animal blood, hair, offal, meat, bones, Hooves, horns,
Scales, Kara and its steamed or dried products, soy sauce, beer grounds, ginger grounds, sake grounds, whiskey grounds, corn starch by-products, potato starch grounds, sweet potato starch grounds, tofu grounds, soy milk grounds, dairy processed grounds, oranges Juice residue, apple juice residue,
Sardines, mackerel, tuna, tangerines, thighs, bamboo shoots, canned corn meal, soybean oil cake, rapeseed oil cake, cottonseed oil cake, peanut oil cake, linseed oil cake, sesame oil cake, castor oil cake, rice bran oil cake, kapok oil Processing residue of food industry such as grounds, coffee grounds, beet pulp, sugarcane by-products, silkworm pupa oil grounds, silk spun pupa litter, tobacco litter, licorice grounds, endju grounds, cattle, pigs, horses and other livestock manure, chicken,
Excess sludge obtained by treating poultry feces such as quails, household waste, and waste liquid containing organic matter by the activated sludge method, and mixtures thereof. Also, soil improvement materials such as vermiculite, zeolite, perlite, bentonite, diatomaceous earth fired granules, peat, charcoal, kuntan, humic acid materials, polyethyleneimine materials, polyvinyl alcohol materials, nitrogenous fertilizers, phosphate fertilizers, Kali fertilizer, organic fertilizer, compound fertilizer, calcareous fertilizer, silicate fertilizer, malay fertilizer,
Ordinary fertilizers based on the Fertilizer Control Law, such as manganese fertilizers, boron fertilizers, and trace element fertilizers, special fertilizers based on the Fertilizer Control Law, such as dust ash, synthetic polymer compounds such as superabsorbent polymers, and metals such as cobalt and nickel ,vitamin,
Organic substances such as sugars, amino acids and nucleic acids, and soils such as Akadama soil and Kanuma soil can also be added to the organic material.

The water content and the carbon / nitro ratio of the organic material to be produced vary depending on the type of the organic material used, but the water content is 20 to 80% by weight, and the carbon / nitro ratio is about 2 to 70 by weight. It is desirable to adjust. The degree of ripening can be determined by the color, smell, and feel of the ripened product. The rotten product is used as it is or after drying.
It can be mixed with a material containing an oma genus microorganism.

A material containing a microorganism of the genus Pharma can be produced according to the production method described in JP-A-6-105617. Here, the microorganisms of the genus Phoma are all Ph-type microorganisms described in JP-A-6-105617.
Oma microorganisms. In addition, Pharma sp. GS
-8-2, Phoma sp. GS-10-2, Ph
oma sp. The color tone and morphological properties of the strain named GS-12-2 after culturing are described in JP-A-6-105617.
The details are described in Japanese Patent Publication No.

[0008] Among the microbial materials of the present invention, 40 organic materials are used.
° C. or higher, preferably when producing a microbial material that is a mixture of a ripened product and a material containing a microorganism of the genus Phoma, which has been aged for 2 days or more at a temperature of 65 ° C. or more, the mixing ratio of both is usually a weight ratio, For the former 100, the latter is 0.0
The ratio is from 1 to 10000, preferably from 0.1 to 1000.

Production method 2 Among the microbial materials of the present invention, a microbial material obtained by mixing a ripened product obtained by aging an organic material at a temperature of 40 ° C. or more, preferably 65 ° C. or more for 2 days or more, and a material containing a microorganism of the genus Phoma. Can be produced by, for example, the following method. The amount and height of the microbial material produced by the method described in Production Method 1 are adjusted and deposited so that oxygen can be sufficiently supplied, and the microorganism material is matured. At this time, the water content is preferably adjusted to about 20 to 70%, and the temperature of the sediment is usually maintained in the range of 10 to 60 ° C, and preferably in the range of 15 to 45 ° C. The degree of ripening may be appropriately set depending on the use of the microbial material, and it is more preferable that the decomposition is continued slowly even after application to the soil, etc., and the color, smell, touch and sedimentation of the microorganisms of the genus Phoma are increased. It can be visually observed and judged.

Next, an application example of the microorganism material of the present invention will be described. Examples of the use of the microorganism material of the present invention include the use of suppressing plant diseases and promoting the growth of plants.

[0011] In such a use, for example, when used for cultivation of field crops such as vegetables and flowers, and turf cultivation, 0.1 to 1,000 kg, preferably 1 to 200 kg, of the microorganism material of the present invention per are are usually applied to the soil surface. Spray. At this time, the microorganism material of the present invention and soil may be mixed. Paddy rice, vegetables,
When mixed with the cultivation soil for raising flowers, the microorganism material of the present invention is mixed usually at a ratio of 0.005 to 50%, preferably 0.05 to 5% with respect to the weight of the cultivation soil. The microbial material of the present invention can be used in any form of powder, granule, and pellet. Further, the microorganism material of the present invention can be applied after being diluted with water. When the microbial material of the present invention is applied after being diluted with water, the dilution ratio is the microbial material of the present invention /
The water ratio is 0.0001 to 0.5, preferably 0.001 to 0.3 by weight, and when applied to field crops such as vegetables and flowers, cultivation of turf, etc., the equivalent of the microorganism material of the present invention is used. so,
It is applied so that it is usually 0.1 to 1,000 kg, preferably 1 to 200 kg per are. The microbial material of the present invention is an insecticide, a nematocide, a fungicide, an acaricide, a herbicide,
They can be mixed with or used in combination with plant growth regulators, soil improvement materials, and the like.

The plant cultivation soil containing the microbial material of the present invention can be produced by mixing the microbial material of the present invention with soil, fertilizer, soil improvement material and the like. For example, after heating and sterilizing the soil and sieving it to an appropriate particle size, nitrogen, fertilizer containing phosphoric acid, kari components and the like, perlite, peat moss and other soil improving materials and the microorganism material of the present invention are mixed,
The pH can be adjusted to around 6-7. Such cultivation soil for plant growth is used, for example, as cultivation soil for growing seedlings such as paddy rice, vegetables, and flowers.

The effect of the microbial material of the present invention on controlling plant diseases can be determined, for example, by mixing the microbial material with soil containing pathogenic bacteria of the plant, then sowing plant seeds, irrigating it appropriately in a greenhouse, and continuing cultivation. However, when the microorganism material of the present invention is not applied and / or when a ripened product obtained by ripening an organic material at a temperature of 40 ° C. or more for 2 days or more is applied instead of the microorganism material of the present invention, and / or The material containing the microorganisms of the genus Phoma, and 4 organic materials.
Judgment of seed germination and seedling establishment is higher than when applied without mixing with a ripened product that has been ripened for 2 days or more at a temperature of 0 ° C. or more. The effect of promoting the growth of plants is, for example, when the microbial material is mixed with soil, seeded with the seeds of the plant, irrigated appropriately in a greenhouse to continue cultivation, and when the microbial material of the present invention is not applied. Alternatively, when a ripened product obtained by ripening an organic material at a temperature of 40 ° C. or more for 2 days or more is applied instead of the microorganism material of the present invention, and / or a material containing a microorganism of the genus Phoma is used instead of the microorganism material of the present invention, Is applied without mixing with a ripened product that has been ripened for 2 days or more at a temperature of 40 ° C. or more,
It can be determined from the fact that the number of leaves, plant height, leaf color, above-ground weight, below-ground weight, fruit weight and the like after germination are superior.

[0014]

The present invention will be described in more detail with reference to production examples and test examples, but the present invention is not limited to these examples.

Production Example 1 Bran 100 was placed in a glass beaker having a volume of 2,000 mL.
g and 90 ml of distilled water, and after mixing, autoclave (1
(21 ° C., 20 minutes) and then cooled to room temperature. 3. Agar plate medium (trade name: potato dextrose agar medium “Nissui”, manufactured by Nissui Pharmaceutical Co., Ltd .; Nissui Pharmaceutical Co., Ltd .; 200 ppm chloramphenicol);
0g, glucose 20.0g, agar 15.0g, total 3
9.0 g, to which 1 L of water is added and dissolved by heating; hereinafter, referred to as PDA-CP medium. ) In an autoclave (121
(20 ° C, 20 minutes), dispensed in 6-mL portions (about 3 mm in depth) into a 9 cm diameter petri dish, cooled to room temperature, and placed in a sterile room.
Pharma sp. GS-12-2 was inoculated into PDA-CP medium and cultured aseptically at 25 ° C for 7 days. Place three pieces of the latter (2 cm on each side) on the surface of the former in a sterile room,
Aseptic stationary culture was performed at 5 ° C. for 2 weeks to obtain Comparative Material A. Surplus sludge (water content: 85.50%) obtained by treating an organic matter-containing waste liquid generated from the food industry such as the ham manufacturing industry by the activated sludge method.
8%) 40 m ³ in the excess sludge deposition rot ripe products (water content 39.
(3%) 80 m ³ was mixed using a tire excavator to adjust the water content to 57.3%. The back wall and both side walls made of concrete and capable of supplying air from the bottom were also deposited in a fermenter made of concrete so as to have a height of 2 m, and forcedly ventilated. The product temperature at a height of 1 m above the ground was 71 ° C one day after deposition, 83 ° C two days later, and 80 ° C three days later. Six days later, the temperature fell below 40 ° C., so the tire was turned back with a shovel car. The mixture was turned over two more times to ripen. The temperature of the product hardly increased, a peculiar rancid smell and ripe color were recognized, and it became sticky to the hands.
After 8 days, a mature product A (water content: 37.0%) was obtained. 2,0
In a 00mL beaker, 100g of comparative material A and ripe A10
0 g was added and mixed uniformly with a spoon to obtain a microorganism material A of the present invention.

[0016] Put the bran 150kg Preparation Example 2 Sterile deposited culture apparatus is adjusted to 60% after the water content sterilized, Phoma s cultured aseptically
p. GS-12-2 was added thereto, and the cells were deposited and cultured at 30 ° C. for 3 days. Then, sterile dry air was sent to dry for 2 days to obtain 90 kg of a comparative material B containing a microorganism of the genus Phoma (water content: 5.0%). 2,000 kg of rotten product A (water content 37.2
%) And 200 kg of well water were added and mixed (water content: 39.1%), and the height was set to 1 m in a rotary stirring fermenter (width 4 m, length 50 m, height 1 m).
Deposited. The stack was turned back after 7, 14, 19, 22, 25, and 30 days so that the product temperature at the top of the deposit did not exceed 50 ° C. On the seventh day, 100 kg of well water was added. On the 35th day, the temperature rise for 5 days after switching back was 1
It was within 0 ° C. On the 7th and 11th day of observation, Pho
ma sp. It was visually confirmed that the white hyphae of GS-12-2 had grown, and a peculiar rancid odor and a ripe color were observed. 996 kg were obtained (water content 34.5%).

Production Example 3 The microorganism material of the present invention B400k
g and 280 kg of well water are added and mixed (water content 40.8
%), And ripened in the same manner as in Production Example 2 to obtain 2,321 kg of the microorganism material C of the present invention (water content: 34.8%).

Microorganism Separation Example 1 90 mL of water was added to 10 g of comparative material A, and the mixture was stirred.
Was placed on a PDA-CP medium and cultured at 25 ° C. for 7 days.
The color tone, tissue and conidial formation structure of the grown filamentous fungi were observed, and it was confirmed that the same species as the inoculated Phoma genus microorganism was growing.

Microbial Separation Example 2 An aqueous extract of the microorganism material B of the present invention produced in Production Example 2 was mixed with a PDA-CP medium or an M40Y agar plate medium (400 g of sucrose, 20 g of malt extract, 5 g of yeast extract, 20 g of agar). , 1 L of water) and isolate the grown filamentous fungi,
Further, the cells were inoculated into a PDA-CP medium and cultured at 25 ° C. for 7 days. Observation of the color tone, tissue, and conidium formation structure of the grown filamentous fungi revealed that three species of Scopulario
A psis microorganism and one Eurotium microorganism have been identified.

Microorganism Separation Example 3 The microorganism material B of the present invention produced in Production Example 2 was applied to a surface of a lawn (variety; Noshiba) of a golf course in an amount of 20 kg per are.
Sprayed. 2.5cm in diameter and 5cm in depth 27 days after spraying
Minutes of soil was collected with a cork borer. After adding 90 mL of water to 10 g of the soil and stirring, 5 mL of the mixture was added to a PDA-CP medium and cultured at 25 ° C. for 7 days. The color tone, tissue, and conidium formation structure of the grown filamentous fungi were observed and identified as the same species as the inoculated Phoma microorganism.

Test Example 1 Pytium aphani, a plant blight fungus
The dermatum was cultured in a bran medium at 27 ° C. for 14 days, and 1% by weight mixed with soil (particle diameter: 2 mm or less) was used as disease-contaminated soil. 100 mL of the disease-contaminated soil was mixed with 0.2% by weight of the microorganism material A of the present invention obtained in Production Example 1 and filled into a 130 mL plastic cup. Five cucumber (cultivar: Shogoin Aonaga Setsunari) was sowed and cultivated in a glass greenhouse at a temperature of 21 to 27 ° C. for 10 days with 5 repetitions. The degree of seedling blight disease was investigated by classifying the seedling into three: normal seedling; 0, abnormally grown seedling; 1, dead and unburied seedling;
In addition, the disease degree of the plot (untreated plot) cultivated in the same manner except that the microorganism material A of the present invention was not applied was investigated. The disease severity and control value were calculated by the following formula, and the control effect was examined.

## EQU1 ## Degree of disease (%) = (number of abnormally growing seedlings × 1 + number of dead and unburied seedlings × 2) / (number of seeds × 2) × 100

## EQU2 ## Control value (%) = (degree of disease occurrence in untreated area−degree of disease in treated area) / degree of disease in untreated area × 100 In this case, the control value of the untreated area is 0.

Test Example 2 A test was conducted in the same manner as in Test Example 1 except that 0.2% by weight of a decay product A was mixed instead of the microorganism material A of the present invention, and the control value of seedling blight was determined.

Test Example 3 A test was carried out in the same manner as in Test Example 1 except that 0.2% by weight of comparative material A was used instead of the microorganism material A of the present invention, and the control value of seedling blight was determined.

Test Example 4 Microorganism material C0.2 of the present invention in place of Microorganism material A of the invention
The test was conducted in the same manner as in Test Example 1 except that the weight% was mixed, and the control value of seedling blight was determined. Table 1 shows the control value of seedling blight of Test Examples 1 to 4.

[Table 1]

Test Example 5 The microorganism material B of the present invention produced in Production Example 2 was sprayed on a lawn (variety: Noshiba) surface of a golf course at 20 kg per are. The test was conducted in 0.5 l / section, 1 repetition system, and the number of lesions of lawn disease (Taiwan baldness) due to spontaneously occurring Rhizoctonia sp. Was counted.

Test Example 6 A test was conducted in the same manner as in Test Example 5 except that the microorganism material B of the present invention was not applied, and the number of bald spots in Taiwan was measured. Test example 5
Table 2 shows the results of and.

[Table 2]

Test Example 7 Microorganism material B of the present invention obtained in Production Example 2 on soil contaminated with bacterial wilt in a glass house in Kojima-gun, Okayama Prefecture
Was applied to the soil after spraying at 20 kg per are. 10 days later, eggplants (variety: 1,000 ryos, rootstock is torero) are striped 210c
m, 268 plants were planted at 75 cm between plants. The test was performed in section 1 4.
Three rounds were performed with one repeat. 199 days after planting, the onset of bacterial wilt was investigated, and the disease rate was calculated by the following formula.

[Equation 3] Diseased disease rate (%) = Number of diseased diseased plants / Total number of strains × 100

Test Example 8 A test was conducted in the same manner as in Test Example 7 except that the microbial material B of the present invention was not applied, and the rate of bacterial wilt disease was calculated. Table 3 shows the results of Test Examples 7 and 8.

[Table 3]

Test Example 9 1% by weight of the microbial material C of the present invention obtained in Production Example 3 was mixed with 500 mL of the disease-contaminated soil prepared according to Test Example 1, and filled in a 288-hole plug tray (10 mL / hole). And
One cucumber was sown and cultivated in a glass greenhouse at a temperature of 21 to 27 ° C. for 14 days. The test was performed in 5 replicates. The control value of seedling blight calculated in the same manner as in Test Example 1 was 64 (0 in the untreated group).

Test Example 10 The microbial material B of the present invention obtained in Production Example 2 was applied to a golf course.
1m on the surface of grass (variety: Noshiba)^Two200g per spray
did. 27 days after spraying, the leaf color of the grass was changed to the standard leaf color
Fuji Film color scale made by Photographic Film Co., Ltd.
)). Values are a minimum of 1 and a maximum of 7,
The higher the value, the darker the green. The test is 50m per section ^Two, One iteration
I went in.

Test Example 11 Test Example 1 was conducted except that the microorganism material B of the present invention was not applied.
The test was performed in the same manner as in the case of 0. Table 4 shows the results of Test Examples 10 and 11. The turf leaf color in Test Example 10 was significantly darker than the turf leaf color in Test Example 11, and the growth promoting effect was high.

[Table 4]

Test Example 12 1% by weight of the microbial material B of the present invention obtained in Production Example 2 was mixed with a seedling culture soil which had been sterilized, granulated and sieved, and a plastic nursery box was cut into quarters. Thing (length 15cm x
(Width 28 cm x depth 3 cm). 30 g of rice (cultivar: Koshihikari) seeds were sown on this, and the temperature was 25-1.
Seedlings were grown in a greenhouse at 5 ° C. for 25 days, and the plant height was measured. The test was performed in triplicate.

Test Example 13 A test was conducted in the same manner as in Test Example 12 except that the microorganism material B of the present invention was applied. Table 5 shows the results of Test Examples 12 and 13.

[Table 5] The index of plant height is a ratio of the plant height (cm) of Test Example 13 to 100.

Test Example 14 Commercially available horticultural seedling raising soil (trade name: Dotaro Sumitomo Forestry Co., Ltd.)
Was mixed with 1% by weight of the microorganism material B of the present invention obtained in Production Example 2 and filled into a 130 ml plastic cup. One cucumber (variety: Shogoin Aonaga Setsunari) was sowed and cultivated in a greenhouse at a temperature of 21 to 27 ° C. for 15 days, and the above-ground dry weight of the cucumber was measured. The test was performed on a five-repeat system.

Test Example 15 A test was conducted in the same manner as in Test Example 14 except that the microorganism material B was not applied. Table 6 shows the results of Test Examples 14 and 15.

[Table 6] In addition, the index of the dry weight was 100 times the dry weight (g) of Test Example 15.
Is the ratio.

Test Example 16 20 kg of the microorganism material B of the present invention obtained in Production Example 2 was sprayed on a radish cultivation field in Maniwa-gun, Okayama Prefecture, mixed with soil, and 10 days later, radish (cultivar: T- 39
7) was sown. Forty-eight days after sowing, 20 radishes were extracted, and the underground fresh weight was measured.

Test Example 17 A test was conducted in the same manner as in Test Example 16 except that the microorganism material B was not applied. Table 7 shows the results of Test Examples 16 and 17.

[Table 7] The index of the fresh weight was 100% of the fresh weight (g) of Test Example 17.
Is the ratio.

Test Example 18 In a tobacco cultivation field in Suzu-gun, Ishikawa Prefecture, a mixture of the microorganism material C of the present invention obtained in Production Example 3 and water mixed at a weight ratio of 1: 9 was applied at 50 g per tobacco. did.
52 days after the application, the onset of the damping-off disease was investigated, and the disease-causing strain rate was calculated by the formula of Equation 3. The number of surveyed shares is 338.

Test Example 19 A test was conducted in the same manner as in Test Example 18 except that the microorganism material C of the present invention was not applied, and the onset of damping-off was investigated, and the diseased strain rate was calculated. The number of surveyed strains is 324. Test example 1
Table 8 shows the results of 8 and 19.

[Table 8]

Test Example 20 In an eggplant field in Higashi-Yatsushiro-gun, Yamanashi Prefecture, the microorganism material C of the present invention obtained in Production Example 3 was applied in an amount of 20 kg per are and then mixed with soil. Fifteen days later, eggplants (variety: Senryo No. 2, rootstock: Tolbum Biggar) were planted. 85 days after application, the wilt disease-causing strains were counted. The number of surveyed stocks is 50.

Test Example 21 A test was conducted in the same manner as in Test Example 20 except that the microorganism material C of the present invention was not applied, and the number of wilt disease-causing strains was counted. The number of surveyed stocks is 50. Table 9 shows the results of Test Examples 20 and 21.

[Table 9]

Test Example 22 In a pepper field in Sanda City, Hyogo Prefecture, the microorganism material C of the present invention obtained in Production Example 3 was sprayed with 20 kg per are and then mixed with soil. Five days later, peppers were planted. 45 days after application, the number of plague-producing strains was counted. The number of surveyed stocks is 7
0 shares.

Test Example 23 A test was performed in the same manner as in Test Example 22 except that the microorganism material C of the present invention was not applied, and the number of plague-producing strains was counted. The number of surveyed stocks is 70. Table 10 shows the results of Test Examples 22 and 23.
Shown in

[Table 10]

Test Example 24 In the eggplant field in Kasai City, Hyogo Prefecture, the microorganism material C of the present invention obtained in Production Example 3 was sprayed with 20 kg per are and then mixed with soil. Ten days later, eggplant (variety: Senryo No. 2, rootstock is Italian Happy) was 210 cm between strips and 70 between stocks.
Planted 9 plants in cm. The test was performed at 13.23 m ² in one section and three times. From 116 days after application to 146 days after application, the harvest fruit weight of the eggplant was measured.

Test Example 25 A rotten product A was used in place of the microorganism material C of the present invention in an amount of 2
The test was carried out in the same manner as in Test Example 24 except that 0 kg was sprayed and then mixed with the soil, and the harvest fruit weight of the eggplant was measured. Table 11 shows the results of Test Example 24 and Test Example 25.

[Table 11]

Test Example 26 In a melon field in Akumi-gun, Yamagata Prefecture, the microorganism material C of the present invention obtained in Production Example 3 was applied in an amount of 17 kg per are and then mixed with soil. 11 days later melon (breed: Andes)
Was planted. Five hundred melons were harvested 100 days after application, the fruit weight of each was measured, and the average value per one was calculated.

Test Example 27 Test Example 26 except that the microorganism material C of the present invention was not applied.
A test was performed in the same manner as described above, and five melons were harvested 100 days after application, the fruit weight of each melon was measured, and the average value per one was calculated. Table 12 shows the results of Test Examples 26 and 27.

[Table 12]

[0048]

The microbial material of the present invention has a high plant disease suppressing effect and a high plant growth promoting effect.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁷ Identification code FI Theme coat ゛ (Reference) C09K 101: 00

Claims (7)

[Claims] 1. A microorganism material comprising a mixture of a ripened product obtained by ripening an organic material at a temperature of 40 ° C. or more for 2 days or more and a material containing a microorganism of the genus Phoma. 2. A microorganism material comprising a mixture of a ripened product obtained by ripening an organic material at a temperature of 65 ° C. or more for 2 days or more and a material containing a microorganism of the genus Phoma. 3. The microorganism material according to claim 1 or 2,
A microbial material characterized by being ripened for more than a day. 4. The microorganism of the genus Pharma.
p. GS-8-2 (Deposited bacteria of the Institute of Biotechnology and Industrial Technology, Ministry of International Trade and Industry, Accession No. FERM P-1300
3), Pharma sp. GS-10-2 (National Institute of Bioscience and Human-Technology deposit bacteria, accession number FERM P-13129) and Phoma sp.
GS-12-2 (Deposited by the Research Institute of Biotechnology and Industrial Technology, Ministry of International Trade and Industry, Accession No. FERM P-13004)
The microorganism material according to claim 1, which is at least one member selected from the group consisting of: 5. A soil for cultivating a plant, comprising the microbial material according to claim 1. 6. A method for controlling plant diseases using the microorganism material according to claim 1. 7. A method for promoting plant growth using the microorganism material according to any one of claims 1 to 4.