JP2000106833A - Product made from grains as raw material, material for promoting proliferation of useful microorganism and their production - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a product capable of maintaining the activity of vitamin Bs, etc., at a high value, readily absorbing minerals, etc., and suitable for raising organisms by inoculating a koji mold to grains, preparing a koji, adding fresh grains thereto, providing mixed grains, treating the resultant mixed grains under specific conditions and then removing phytic acid. SOLUTION: A koji mold is inoculated into grains such as soybean or rice to prepare a koji and fresh grains are then added to a product obtained by the treatment for preparing the koji to afford mixed grains. Water is further added thereto to hydrolyze proteins in the mixed grains and phytic acid in the mixed grains is removed to provide a product using the grains as a raw material. Grains are preferably subsequently added to the resultant product to afford additionally mixed grains and water is then preferably added thereto to hydrolyze proteins in the additionally mixed grains and remove the phytic acid. Thereby, the product is preferably prepared. In the hydrolyzing step, the proliferation of useful microorganisms such as fungi, lactic acid bacteria or bifidus bacteria and/or useful microorganisms added to the product is preferably promoted to produce a material for promoting the proliferation of the useful microoraanisms.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a cereal-based product, a useful material for promoting the growth of microorganisms, and a method for producing the same.

In the present invention, cereals are soybeans, rice,
It means wheat, corn, these meals, processed products from cereals, and the like, and at least one of these cereals is used as a raw material substrate. The cereal-based product means food (eg, tofu or soy milk), livestock feed, aquaculture feed, and the like using the cereal as a raw material.

[0003] In the present invention, useful microorganism growth promoting materials include useful microorganisms (fungi such as yeast, lactic acid bacteria, bifidobacteria and other useful microorganisms) which effectively act to maintain the health of living organisms in breeding organisms. Fungi).

[0004]

2. Description of the Related Art In general,
There is a great demand for cereal-based products and a wide variety of products is provided.

[0005] Conventionally, brews made using koji mold, especially those made from cereals, include sake, vinegar,
There were soy sauce and miso.

However, most of them are palatable foods, and conventionally, for the purpose of nutrition and health maintenance,
It is no exaggeration to say that there was no food that could be eaten in large quantities.

[0007] To further explain, the function of koji mold in conventional brewed products is mainly used for saccharifying starch in sake, shochu, vinegar and the like, and is used for decomposing proteins in soy sauce and miso. Was. It is also difficult to commercialize these brews using only koji molds, and it is also known that each of these brews is made by utilizing the functions of lactic acid bacteria, yeast, and the like. This is to utilize the characteristics of the koji mold. In other words, it can be said that this has become possible because the koji mold has the property of coexistence and symbiosis with lactic acid bacteria, yeast and the like. The function of lactic acid bacteria and yeast in sake, soy sauce, miso, and the like is to suppress the growth of putrefactive bacteria during aging for a relatively long period of time.Lactic acid bacteria grow to produce lactic acid, and yeast is alcoholic under anaerobic conditions. The production of each product has been achieved in order to produce

[0008] As described above, the products utilizing the koji mold are each produced through the process of coexistence and symbiosis of the koji mold with lactic acid bacteria and yeast. There was no brewed food for the purpose of maintaining the health of humans and animals, such as the intestinal action.

[0009] Health foods using lactic acid bacteria include yogurt, but yogurt is not a food using koji mold.

On the other hand, in recent years, many antibiotics (antibiotics) have been put into practical use since the clinical application of penicillin in 1941, and greatly contributed to infectious diseases. MRSA
(Methicillin-resistant Staphylococcus aureus) and drug-resistant bacteria such as VRSE (vicomycin-resistant Staphylococcus epidermidis). Humans and domestic animals are administered the same antibiotics due to zoonotic diseases, and drug-resistant bacteria have been confirmed in many pig farms and poultry farms, which has become a major social problem. At present, there is no solution for these.

With respect to the coexistence and symbiosis of microorganisms, many studies have been made with particular attention to the function of lactic acid bacteria, but it seems that there is still no use other than yogurt as a dairy product. Regarding lactic acid bacteria, apart from the aforementioned suppression of the growth of putrefactive bacteria due to a decrease in pH due to the production of lactic acid, attention has also been paid to the action of antibacterial substances such as bacteriocin and mucous polysaccharides.

[0012] It is also said that research results on physiological activity, which is the potential ability of lactic acid bacteria, can be expected in the future.

Therefore, suppression of the growth of harmful bacteria (causes of infectious diseases, etc.) by the function as a probiotex does not cause the emergence of resistant bacteria such as the administration of antibiotics. It seems that it can be a countermeasure.

Further, it has been proposed to suppress the growth of Salmonella in the intestine of livestock by useful microorganisms mainly composed of lactic acid bacteria (see Japanese Patent Application Laid-Open No. 9-506625). In this conventional example, attention was paid to the fact that a symbiosis of 29 kinds of bacteria mainly composed of lactic acid bacteria cultured in the cecal contents of poultry produces short-chain fatty acids which Salmonella dislikes, and the growth of Salmonella in the intestine of livestock. Are proposed to suppress symbiosis. This conventional example was achieved by culturing a symbiotic bacterium using the contents of the cecum as a nutrient source, so that these bacterial groups stay in the intestine, produce short-chain fatty acids, and produce Salmonella spp. Probably, it could be expected that the function of suppressing the proliferation was sufficiently exhibited.

However, in this conventional example, the following inconvenience occurs because the cecal contents of chickens are preliminarily cultured in order to make the bacteria work in the intestine. In other words, the cecal contents of chickens must be given due consideration to hygiene, extremely strict hygiene management is required for the culture of bacterial symbiotic organisms, and their versatility in application is poor.
Unsuitable for food applications.

Therefore, unlike a nutrient source having a hygienic problem such as chicken cecal contents, it is excellent in hygiene, easy to produce, and has a low production cost. There is a demand for the development of a material and the development of a technique capable of simultaneously growing useful microorganisms during the production of the useful microorganism growth promoting material.

In addition, a product using cereals as a raw material and capable of efficiently mass-producing a very high-quality product such as phytic acid removed, or a material capable of mass-producing efficiently as the useful microorganism growth promoting material Was also requested.

The present invention has been made in view of the above points, and has been made so that the present applicant has already proposed in Japanese Patent Application Laid-Open No. Hei 7-23725 a mass-produced product using cereals as a raw material. Utilizing coexistence and symbiosis with lactic acid bacteria and yeasts contained in Aspergillus oryzae.
No. 725, which is a useful microorganism growth promoting material obtained by using a product using cereals as a raw material, and which effectively acts to maintain the health of living organisms in growing the living organisms It is an object of the present invention to provide a cereal-based product, a useful microorganism growth-promoting material, and a method for producing the same, which enables more efficient mass production of a useful microorganism growth-promoting material for promoting the growth of cereals.

Another object of the present invention is to provide a useful microorganism growth-promoting material which is excellent in hygiene, is easy to produce, and is a low-cost nutrient source. An object of the present invention is to provide a production method capable of simultaneously growing useful microorganisms.

[0020]

SUMMARY OF THE INVENTION The present applicant has disclosed in Japanese Patent Application Laid-Open No. Hei 7-23725 that the phytic acid in cereals can be easily removed while keeping the cereal in a solid state. It is possible to obtain a product that maintains the activity of vitamin B and the like contained in the product at a high level, easily absorbs the minerals contained in the product, and can further promote the absorption. In addition, a product using cereals as a raw material, which has a low production cost, and a method for producing the same are proposed.

In order to achieve the above object, the present inventor has conducted intensive studies on the cereal-based product proposed by the present applicant in the above-mentioned gazette. A very high-quality product made from cereals by adding new cereals to the product and hydrolyzing it as a mixed cereal, or adding the cereals to the product thus produced and hydrolyzing it as an additional mixed cereal It has been found that the present invention can be mass-produced more efficiently, and the present invention has been completed. In addition, regarding the useful microorganism growth promoting material, by incorporating the technology of probiotex, the product using the cereal as a raw material can promote the growth of useful microorganisms that effectively act to maintain the health of living organisms, It is found that the koji mold and the useful microorganism coexist and coexist with the product using the cereal as a raw material to promote growth, and a new cereal is added to the koji-produced product to hydrolyze as a mixed cereal. By adding cereals to the useful microbial growth-promoting material thus produced and then hydrolyzing it as an additional mixed cereal, it is possible to mass-produce a very high-quality useful microbial growth-promoting material from cereals more efficiently. The present invention has been completed to complete the present invention.

More specifically, the term "probiotex" as used herein means that koji is inoculated into cereals to produce koji, and the koji-produced product is hydrolyzed to hydrolyze proteins and / or carbohydrates in the product. It is produced by decomposing and removing a predetermined amount of phytic acid in the cereal. In this process, an environment is provided in which useful microorganisms such as lactic acid bacteria, yeast, and bifidobacteria can coexist and coexist. That is,
Lactic acid bacteria, yeasts, bifidobacteria, and the like are based on the fact that they can be actively proliferated while coexisting and coexisting with the koji mold on the same product as the same substrate.

The present inventor has further studied diligently and found that the useful microorganisms including lactic acid bacteria which grew actively while coexisting in the product can (1) be resident in the intestine of humans and livestock, 2) It has been found that it has no adverse effect on humans and livestock hosts, and (3) it is possible to efficiently produce short-chain fatty acids and the like in the intestine. And found that it contains a substance that is not digested (undigested substance = undegraded enzyme) up to the large intestine, which is a source of nutrients and can produce short-chain fatty acids that are harmful to harmful bacteria such as Salmonella, The present invention has been completed. Further, by combining the product with resistant starch (starch and partially hydrolyzed starch that are not digested and absorbed in the small intestinal cavity of a healthy individual), the undegraded enzyme and resistant starch are combined. Have been found to be a nutrient source such as lactic acid bacteria that can be grown in the intestine of domestic animals, and completed the present invention. Originally, probiotics are living microbial cells, for example, there are products in the form of a mixture of koji mold, yeast, lactic acid bacteria, etc., but as in the present invention, koji mold, yeast, lactic acid bacteria, etc. are used on the same substrate (cereals). ), And there is no commercialized product containing the cells themselves.

As described above, brewed products (sake, miso, soy sauce, etc.) using koji mold are products obtained by coexisting and coexisting koji mold, lactic acid bacteria, and yeast, which are effective in terms of product quality. Method, not the purpose of probiotics. The fact is that in the case of sake and soy sauce, the viable bacteria in the product are sterilized, and even in the case of miso, viable bacteria are often a problem in terms of product management. In addition, since these brewed products have a long preparation and aging period, transition of each microorganism occurs, and it is impossible to produce a product obtained by culturing a large amount of koji mold, yeast, lactic acid bacteria, etc. in the same substrate (cereals).

The product based on the cereal of the present invention described in claim 1 based on these research results is produced by the production method described in claim 7,
The cereals are inoculated with a koji mold to make koji, and a new cereal is added to a product obtained by the koji-making process to form a mixed cereal, and the mixed cereal is hydrolyzed to hydrolyze the protein in the mixed cereal and mix the cereal. It is produced by removing phytic acid in cereals.

Since the cereal-produced product of the present invention is produced in this manner, the koji-producing process allows the koji mold to grow to remove phytic acid in the cereal, and is further produced by the koji-producing process. Enzymes such as phytase and phosphatase in the resulting product ensure the degradation of proteins and phytic acid in newly added grains as well as the grains as the first raw material, resulting in large amounts of mixing. The protein can be reliably degraded and the phytic acid can be efficiently removed from the cereal, and a very good cereal-based product can be obtained.

[0027] A cereal product according to the second aspect is produced by the production method according to the eighth aspect, and a cereal product using the cereal according to the first aspect. The additional mixed cereal is added to the additional mixed cereal, and the additional mixed cereal is hydrolyzed to hydrolyze the protein in the additional mixed cereal and to remove phytic acid in the additional mixed cereal. Features.

As described above, the cereal-based product according to claim 1 contains enzymes such as phytase and phosphatase having enzyme activity.
Protein degradation and phytic acid removal in the initial and additional grains, as well as the newly added grains, will be ensured, and the protein degradation will occur for a large amount of additional mixed grains. And the removal of phytic acid can be performed more reliably and more efficiently, and a product using extremely high-quality cereals as a raw material can be obtained.

Further, the useful microorganism growth promoting material of the present invention according to claim 3 based on the above research results comprises:
A cereal is inoculated with a koji mold to make koji, and a new cereal is added to a product obtained by the koji-making process to form a mixed cereal, and the mixed cereal is hydrolyzed to hydrolyze proteins and / or carbohydrates in the mixed cereal. And by removing a predetermined amount of phytic acid from the cereal. When added to foods containing useful microorganisms, the useful microorganism growth promoting material provides nutrition to the useful microorganisms, coexists with koji mold, promotes the growth of the useful microorganisms, and has the useful microorganisms. Various health-promoting effects can be exerted, the growth of harmful bacteria can be reliably suppressed, and the maintenance of the health of organisms that eat the food can be improved. In addition, this product contains a substance that is not digested in the intestine (undigested substance = undegraded enzyme), which is a nutrient source for lactic acid bacteria and the like in the intestine of livestock and enables the production of short-chain fatty acids that Salmonella bacteria dislike. So
Useful microorganisms, such as lactic acid bacteria, generate undigested nutrients as a nutrient in the intestine of animals and produce short-chain fatty acids that Salmonella bacteria dislike well, thereby suppressing the growth of Salmonella bacteria. Moreover, unlike the above-mentioned conventional example, the chicken and the contents of the cecum are not used as a nutrient source, so that it is extremely excellent in hygiene.

Further, when a resist starch is added to the product to provide a useful microbial growth promoting material, the undegraded enzyme contained in the product and the resist starch are combined to form lactic acid bacteria and the like in the intestine of livestock. , And useful microorganisms such as lactic acid bacteria can produce more undesired short-chain fatty acids in the intestine of animals by using undigested nutrients as a nutrient source, thereby suppressing the growth of Salmonella more reliably. .

Further, as described in claim 4 and claim 5, the useful microorganism growth promoting material of the present invention comprises a useful microorganism contained in a product obtained by the koji-making process and / or a material obtained by the koji-making process. It is characterized in that the useful microorganism added to the product is promoted for growth during the hydrolysis. In this useful microorganism growth promoting material,
During the hydrolysis step, the koji mold and the useful microorganism coexist and coexist with the product having the same substrate, and the useful microorganism is provided with nutrients from the product and is promoted to grow. Are cultured together. Therefore,
If this useful microorganism growth promoting material is added to food, the useful microorganisms already cultured in the useful microorganism growth promoting material and the useful microorganisms contained in the food are further promoted, and various health possessed by the useful microorganism are improved. The promoting action can be exerted and the growth of harmful bacteria can be surely suppressed, and the maintenance of the health of the organism that eats the food can be improved. The useful microorganisms that have already been cultivated here refer to the useful microorganisms naturally required during the production process including the lactic acid bacteria and the like that require mevalonic acid and produce bacteriocin, and the useful microorganisms added in the hydrolysis process. That is.

[0032] The method for producing a useful microorganism growth promoting material of the present invention according to claim 9 is characterized in that a cereal is inoculated with a koji mold to produce koji,
A new cereal is added to the product obtained by the koji-making process to obtain a mixed cereal, and the mixed cereal is hydrolyzed to hydrolyze the protein and / or carbohydrate in the mixed cereal and to reduce a predetermined amount of phytic acid in the cereal. The method is characterized in that a useful microorganism growth promoting material for promoting the growth of the useful microorganism is produced by removing the material.

According to the method for producing a useful microorganism growth promoting material of the present invention according to the ninth aspect, koji is inoculated into a cereal as a raw material to produce koji, and the phytate in the cereal is grown. Is removed, and a new cereal is added to the product of the koji-making process to form a mixed cereal, and the mixed cereal is hydrolyzed to hydrolyze the protein and / or carbohydrate in the mixed cereal and simultaneously to give a predetermined amount of phytic acid. In order to remove phytate and phytic acid, not only the first raw material grains but also newly added grains are reduced by enzymes such as phytase and phosphatase in the product produced by the koji-making process. Removal will be performed reliably, and eventually a large amount of mixed grains can be reliably and efficiently subjected to protein degradation and phytic acid removal,
Further, a predetermined amount of phytic acid can be easily and reliably removed in a short time while the state of the cereal remains in a solid state, and further, the growth of useful microorganisms is promoted, Not only exerts its health promotion effect,
A useful microbial growth promoting material capable of reliably suppressing the growth of harmful bacteria and improving the maintenance of living organisms can be manufactured, the manufacturing process is simple, and the manufacturing cost is low.

According to the method for producing a product using cereals of the present invention according to claim 10, in the hydrolysis step in claim 9, the product contained in the product obtained by the koji making process is included. The present invention is characterized in that the growth of useful microorganisms and / or useful microorganisms added to the product of the koji-making process is promoted.

According to the method for producing a useful microorganism growth promoting material of the present invention according to the tenth aspect, the koji mold and the useful microorganism coexist and coexist with the product having the same substrate during the hydrolysis step, and the useful microorganism Is promoted by receiving nutrients from the product, and the product is in a state in which koji mold and useful microorganisms are cultured together. Therefore, if this useful microorganism growth promoting material is added to food, useful microorganisms already cultured in the useful microorganism growth promoting material and useful microorganisms contained in the food are further promoted, and the useful microorganism has Various health-promoting effects can be exerted, the growth of harmful bacteria can be reliably suppressed, and the maintenance of the health of organisms that eat the food can be improved. The process of producing the useful microorganism growth promoting material acting as described above is simple, and the production cost can be reduced.

The useful microorganism can be at least one of fungi, lactic acid bacteria, and bifidobacteria, and can be appropriately selected according to the use of the useful microorganism growth promoting material.

The useful microorganism growth promoting material according to claim 6 is produced by the production method according to claim 12, and is useful according to any one of claims 3 to 5. A cereal is further added to the microbial growth promoting material to form an additional mixed cereal, and the additional mixed cereal is hydrolyzed to hydrolyze proteins in the additional mixed cereal and to remove phytic acid in the additional mixed cereal or to remove useful microorganisms. Characterized by being produced by growing.

As described above, the useful microorganism growth promoting material according to any one of claims 3 to 5 contains enzymes having phytase, phosphatase and the like having enzymatic activity and is useful. Microorganisms are also contained, so that not only the initial raw material grains and newly added grains, but also the degradation of proteins and the removal of phytic acid in the added grains are reliably performed, and the growth of useful microorganisms is also achieved. It will be able to be performed reliably,
Furthermore, protein degradation, phytic acid removal and growth of useful microorganisms can be reliably and more efficiently applied to a large amount of additional mixed grains, and a very good quality useful microorganism growth promoting material can be obtained.

[0039]

FIG. 1 is a block diagram showing an embodiment of the present invention.
3 will be described.

FIG. 1 shows a product of the present invention as a cereal raw material and a method for producing a useful microorganism growth promoting material.

First, a case of producing a product using wheat bran as a raw material and a cereal raw material will be described. Since this wheat bran contains a large amount of plant-derived phytase capable of decomposing phytic acid in a raw state, it is suitable as a cereal that degrades phytic acid in a cereal field.

Explaining in accordance with the process shown in FIG. 1, the wheat bran having a high phytase activity is not steamed and its water content is set to an amount which allows the koji mold to grow (for example, 35% by weight).

The method of the present invention is carried out on the wheat bran whose water content has been adjusted as described above, as follows.

That is, a seed koji made of Aspergillus oryzae is inoculated into a wheat bran alone at a predetermined weight ratio, and mixed until both are uniform.

Thereafter, the mixture is charged into a koji making apparatus,
The wheat bran having a low water content of about 35% by weight is fermented with a koji mold and kept at a temperature of 28 to 30 ° C. for a predetermined time, and the koji-making is continued until the phytic acid in the wheat bran is sufficiently reduced. Do.

In this case, the phytase or phosphatase, which is an enzyme that decomposes phytic acid, which is produced by the koji mold as the koji mold grows on the wheat bran, decomposes and removes the phytic acid in the wheat bran, and reduces the plant-derived phytase activity of the wheat bran. It also exerts a synergistic effect that greatly improves.

That is, an enzyme that decomposes phytic acid from phytic acid, which is a compound in which all of the hydroxyl groups of myo-inositol have a phosphate group bonded thereto, releases the phosphate group to form inositol pentaphosphate and inositol tetraphosphate. , Inositol triphosphate, inositol diphosphate, inositol monophosphate, and / or inositol are produced to remove the phytic acid.

The phytase activity of the wheat bran was 2465.9 u / g before the koji-making treatment, but was greatly improved to 3860.6 u / g after the koji-making treatment. By the koji making treatment according to the present invention, phytic acid in cereals can be more efficiently decomposed and removed.

The koji mold used in the koji-making is an aspergillus oryzae which has been used in fermented foods and tempe peculiar to Japan since ancient times, and is safe as a food. Aspergillus oryzae, Aspergillus niger, and other Aspergillus and Rhizopus genases having a high phytase titer and phosphatase titer may be used. Mutants of these koji molds and genetically modified koji molds can also be used.

[0050] The fermentation time is at least 24 hours or more depending on the type of koji mold used, and may be a fermentation time sufficient to sufficiently remove phytic acid from soybean meal.

Thereafter, the koji-treated product is
As a new cereal, for example, a predetermined amount of tofu cake is added and mixed to obtain a mixed cereal.

Thereafter, the mixed cereal was added with water until its weight ratio became about 50%, and then maintained at a temperature of 30 to 45 ° C. for a predetermined period of time, thereby decomposing phytase and phosphatase contained in the product. Phytic acid contained in mixed grains is sufficiently reduced.

As a result, phytic acid in the mixed cereal consisting of wheat bran and tofu cake was completely removed.

According to this method, the growth of lactic acid bacteria is very easy, and the growth of lactic acid bacteria suppresses the growth of various bacteria in the mixed cereals. Goods can be mass-produced. Also,
Sufficient phytic acid removal treatment can be performed without reducing the water content.

The removal of phytic acid is carried out by releasing at least one phosphate group from phytic acid consisting of inositol hexaphosphate.
Inositol tetraphosphate, inositol triphosphate, inositol diphosphate, inositol monophosphate, and inositol from which the phosphate group of the group has been released have water solubility and are contained in products made from cereals. It has the effect of greatly promoting the absorption of minerals such as calcium.

To further explain, the inositol hexaphosphate and inositol pentaphosphate have strong ionic bonds,
It does not elute the bound calcium and greatly suppresses the calcium absorption action. On the other hand, inositol tetraphosphate to inositol monophosphate have an appropriate affinity to bind calcium well and easily elute the bound calcium when necessary. The characteristic action of promoting is shown.

The soybean meal in which phytic acid has been greatly or almost completely reduced according to the present invention is useful as a vitamin B or the like contained in soybean meal which has a growth promoting action and an anti-fatty liver action. The activity is maintained at a high level, and the soybean meal is easy to absorb minerals and the like contained in the soybean meal.

Since the cereal-based product of the present invention is produced in this manner, the koji-producing process is used to grow the koji mold to remove phytic acid in the cereal, and the koji-producing process further produces the product. Enzymes such as phytase and phosphatase in the resulting product ensure the degradation of proteins and phytic acid in newly added grains as well as the grains as the first raw material, resulting in large amounts of mixing. The protein can be reliably degraded and the phytic acid can be efficiently removed from the cereal, and a very good cereal-based product can be obtained.

In the above description, the koji-making process of wheat bran and the subsequent production and hydrolysis of mixed grains are performed continuously at the same place. Once the product is taken out, the product may be transported to another place where the mixed cereal is produced and hydrolyzed, and the mixed cereal is produced and hydrolyzed at another place.
When the mixed cereal is performed in another place, the moisture and temperature of the mixed cereal are adjusted to appropriate values after the mixed cereal is formed, and the inside of a hydrolysis container, for example, a flexible container that can be moved is removed. It is good to do it with care. In this way, the product obtained by the koji-making treatment of wheat bran has a high phytic acid decomposability, and has a property of easily promoting the growth of lactic acid bacteria, and has a property of suppressing the growth of various bacteria. The production and hydrolysis of mixed cereals performed elsewhere will be performed very well, as in the case of continuous processing.

In the case where the thus-produced wheat bran having plant-derived phytase and the product using tofu meal as a raw material cereal are used as a feed or the like, as shown in FIG. The cereal product without phytic acid without drying the product thus produced is dried, and then crushed to obtain phytic acid-removed crushed cereals to produce livestock feed and aquaculture feed. It may be a product such as a raw material. In particular, when used as feed for livestock, phytic acid is completely degraded, so phytic phosphorus is converted into inorganic phosphorus and eluted, and is easily absorbed well into the body. Phosphorus, which is a nutrient contained in tofu cake, is absorbed into the body, which can increase nutrients, and furthermore, phosphorus is not excreted in feces of livestock, making it less likely that livestock will become ill. Is exhibited.

As described above, according to the present invention, live cereals are grown to remove phytic acid in cereals and to hydrolyze proteins. However, phytic acid can be easily removed, the manufacturing process is simplified, and the manufacturing cost is reduced.

Therefore, according to the present invention, the following problems occurred in the case of producing a solid product by the conventional method,
That is, there is a need for a step of turning the raw material into a liquid, and further a post-step of turning the liquid into a solid after the decomposition treatment of phytic acid is required, which leads to problems such as a complicated manufacturing process and high cost. It can be surely eliminated.

In the present invention, a product obtained by subjecting a product obtained as a raw material of the mixed cereal obtained by the hydrolysis step to drying or a product not subjected to drying to a fresh cereal is used. For example, a predetermined amount of tofu lees is added and mixed to form an additional mixed grain, and thereafter, water is added to the additional mixed grain until the weight ratio becomes about 50%, and then 3
It is kept at a temperature of 0 to 45 ° C. for a predetermined period of time, and the phytase contained in the product is sufficiently reduced by the decomposing action of the phosphatase to reduce the phytic acid contained in the additional mixed cereal, and to reduce the hydrolysis. Do.

Regarding the hydrolysis of this protein, a hydrolysis time and a hydrolysis temperature sufficient to sufficiently remove phytic acid from the additional mixed cereals may be used depending on the type of koji mold used.

As a result, phytic acid in the product obtained from the mixed cereal consisting of wheat bran and tofu lees and the additional mixed cereal consisting of fresh tofu lees was completely removed.

Next, a method for producing a useful microorganism growth promoting material will be described.

FIG. 1 shows one embodiment of a production method for producing a useful microorganism growth promoting material from soybean meal, which is a kind of cereal, according to the present invention in the solid line portion, and further shows the production method of the present invention in the chain line portion in FIG. 2 shows another embodiment of the present invention.

First, an embodiment of the method of the present invention will be described along the steps shown by the solid lines in FIG.

First, soybean meal as raw cereals is steamed. By performing this steaming, the growth of the koji mold becomes easy. Further, the soybean meal is preferably cooked by a batch system or a continuous system depending on the purpose of production and the like.

Then, the soybean meal after the completion of the steaming is cooled once so that the moisture content in the soybean meal is adjusted to an amount (for example, 40% by weight) at which the koji mold can grow.

The method of the present invention is carried out as follows on the soybean meal whose water content has been adjusted as described above.

That is, the soybean meal alone after steaming is inoculated with a seed koji made of koji mold at a predetermined weight ratio, and mixed until both are uniform.

Thereafter, the mixture is charged into a koji making apparatus,
Koji-making is carried out by keeping the soybean meal having a low water content of 40% by weight by fermenting the soybean meal with a koji mold while keeping the temperature at 28 to 30 ° C. for a predetermined time, and reducing phytic acid in the soybean meal sufficiently. .

In this case, an enzyme that decomposes phytic acid called phytase or phosphatase produced by the koji mold as the koji mold grows on the soybean meal decomposes and removes phytic acid in the soybean meal.

That is, an enzyme that decomposes phytic acid from phytic acid, which is a compound in which all of the hydroxyl groups of myo-inositol have a phosphate group bonded thereto, releases the phosphate group to form inositol pentaphosphate and inositol tetraphosphate. , Inositol triphosphate, inositol diphosphate, inositol monophosphate, and / or inositol are produced to remove the phytic acid.

As the koji mold used in the koji making, the koji mold described above is preferably used.

The fermentation time is at least 24 hours or more depending on the type of koji mold used, and may be a fermentation time sufficient to sufficiently remove phytic acid in soybean meal.

Thereafter, the koji-produced product is
As a new cereal, for example, a predetermined amount of tofu cake is added and mixed to obtain a mixed cereal.

Thereafter, water was added to the mixed cereal until the weight ratio became about 50%, and the mixture was kept at a temperature of 30 to 45 ° C. for a predetermined period of time to decompose phytase and phosphatase contained in the product. Phytic acid contained in the mixed grains is sufficiently reduced and hydrolysis is performed.

Regarding the hydrolysis of this protein, a hydrolysis time and a hydrolysis temperature sufficient to sufficiently remove phytic acid from soybean meal may be used depending on the type of koji mold used.

According to this method, an organic acid is generated at the beginning of fermentation to suppress the growth of various bacteria in soybean meal, and there is no fear of secondary contamination, and a large amount of a product using soybean meal is produced. be able to. In addition, a sufficient phytic acid removal treatment can be performed without using low moisture.

The removal of phytic acid is carried out by releasing at least one phosphate group from phytic acid consisting of inositol hexaphosphate.
Inositol tetraphosphate, inositol triphosphate, inositol diphosphate, inositol monophosphate, and inositol from which the phosphate group of the group has been released have water solubility and are contained in products made from cereals. It has the effect of greatly promoting the absorption of minerals such as calcium.

To further explain, the inositol hexaphosphate and inositol pentaphosphate have strong ionic bonds,
It does not elute the bound calcium and greatly suppresses the calcium absorption action. On the other hand, inositol tetraphosphate to inositol monophosphate have an appropriate affinity to bind calcium well and easily elute the bound calcium when necessary. The characteristic action of promoting is shown. In this case, by adjusting the fermentation time and hydrolysis time and hydrolysis temperature according to the type of grain, state, characteristics, amount, type of koji mold, state, characteristics, amount, type of product, characteristics, etc., It is advisable to control the number of phosphate groups released from phytic acid.

When the useful microorganism growth-promoting material of this embodiment thus produced is added to food containing useful microorganisms, it provides nutrients to the useful microorganisms and coexists with the koji mold. Promote the growth of useful microorganisms, while exhibiting various health promoting effects of the useful microorganisms, it is possible to reliably suppress the growth of harmful bacteria,
The maintenance of the health of the organism that eats the food can be improved.

Next, another embodiment of the method of the present invention for a useful microorganism growth promoting material will be described with reference to the portion shown by the chain line in FIG.

In the present embodiment, in the hydrolysis step, the koji mold and useful microorganisms coexist and coexist with the product produced by the koji-making process and the mixed cereal consisting of newly added cereals. It is intended to be.

Therefore, the same steps as in the above embodiment are performed until the mixed cereal is produced. Then, during the hydrolysis, add useful microorganisms to the product of the koji making process,
The useful microorganisms are put into the product with the naturally occurring useful microorganisms contained in the cereals used as raw materials.

Examples of useful microorganisms include fungi such as yeast, lactic acid bacteria, bifidobacteria, and other useful fungi that are effective in maintaining the health of living organisms in growing them. Good. The fungi, lactic acid bacteria and bifidobacteria are very compatible with cereals as a substrate,
It grows using cereals as a nutrient source. And as the kind of useful microorganism to be put into the product by the koji making process,
At least one type may be used depending on the type of cereal serving as a substrate, the type of food as a final product to be produced, the use, and the like.

When the useful microorganisms are introduced into the mixed cereals and the hydrolysis step is started in this way, the koji mold grown in the koji-making process during the hydrolysis step and the useful microorganisms coexist in the mixed cereals having the same substrate. Symbiosis, the useful microorganisms are provided with nutrients from the mixed cereal, which is a product, and are promoted to grow. As a result, the mixed cereal is mixed with the koji mold and the useful microorganism. In particular, vitamin Bs are produced in the hydrolysis process in the product of the koji-making process, and high nutrient components that are easily absorbed by the useful microorganisms are produced. Is promoted. Finally, a useful microbial growth promoting material is produced using a probiotic cultivated on a product in which the koji mold and the useful microorganism are the same substrate.

When the useful microorganism growth promoting material of the present embodiment thus produced is added to food, useful microorganisms already cultured in the useful microorganism growth promoting material or useful microorganisms contained in the food are added. Is further promoted, and various health promoting effects of the useful microorganism are exhibited, the growth of harmful bacteria can be surely suppressed, and the maintenance of the health of organisms eating the food can be improved. it can. In particular, lactic acid bacteria have the property of activating the physiological activity in the body when inoculated into a human or animal body, even in a dead state as well as a viable state, and are extremely remarkable. Exercise health.

In the present invention, a product obtained by drying the product obtained from the mixed cereal obtained by the above-mentioned hydrolysis step or a product obtained by subjecting the product not dried to a new cereal For example, a predetermined amount of tofu lees is added and mixed to form an additional mixed grain, and thereafter, water is added to the additional mixed grain until the weight ratio becomes about 50%, and then 3
It is kept at a temperature of 0 to 45 ° C. for a predetermined period of time, and the phytase contained in the product is sufficiently reduced by the decomposing action of the phosphatase to reduce the phytic acid contained in the additional mixed cereal, and to reduce the hydrolysis. Do.

[0092] Regarding the hydrolysis of this protein, a hydrolysis time and a hydrolysis temperature sufficient to sufficiently remove phytic acid from the additional mixed cereals may be used depending on the type of koji mold used.

When the useful microbial growth promoting material of this embodiment produced as a raw material for a larger amount of the additional mixed cereal as described above is added to a food containing useful microorganisms, Nutrients, coexistence and coexistence with Aspergillus, promote the growth of the useful microorganisms, while exerting various health promoting effects of the useful microorganisms, it is possible to reliably suppress the growth of harmful bacteria, The maintenance of the health of the organism that eats the food can be improved.

Further, the same steps as those in the above embodiment are carried out until additional mixed grains are produced. Then, during the hydrolysis, useful microorganisms are added to the product obtained by the koji-making process, and / or the grains used as raw materials are added. The useful microorganism-promoting material may be produced by adding a useful microorganism derived from a naturally occurring useful microorganism contained in the product to the product.

In this way, when the useful microorganism is used as a raw material in a larger amount of additional mixed grains to start the hydrolysis step, the koji mold grown in the koji-making process during the hydrolysis step and the useful microorganism have the same substrate. Coexisting and coexisting with the additional mixed cereal, the useful microorganisms were given nutrients from the additional mixed cereal as a product to promote their growth, and the additional mixed cereal was cultured with the koji mold and useful microorganisms together. State. In particular, in the product of the koji-making process, vitamin B is generated in the hydrolysis step, and a nutritive component that is easily absorbed by useful microorganisms is generated.
Using these as a nutrient source, the growth of useful microorganisms is promoted.
Finally, a useful microbial growth promoting material is produced using a probiotex cultured on a product in which the koji mold and the useful microorganism are the same substrate.

When the useful microbial growth promoting material of this example produced in this manner is added to food, the useful microorganisms already cultured in the useful microbial growth promoting material or the useful microorganisms contained in the food are added. Is further promoted, and various health promoting effects of the useful microorganism are exhibited, and infectious diseases can be prevented by surely suppressing the growth of harmful bacteria such as Salmonella. It can improve the maintenance of health.
In particular, when lactic acid bacteria are inoculated into humans or animals, not only in the state of live bacteria but also in the dead state,
It has the property of activating physiological activity in the body, and exerts a very remarkable health promoting action.

When the soybean meal produced as described above is used as feed or the like, as shown in FIG.
The soybean meal produced as in each of the above embodiments may be dried, and then ground to be used as ground soybean meal.

As described above, according to the present invention, a living koji mold is multiplied to remove phytic acid in cereals, and the product is further reduced in molecular weight by hydrolysis. A useful microorganism growth promoting material can be obtained by utilizing the same, and a useful microorganism growth promoting material that facilitates the growth of useful microorganisms that effectively act to maintain the health of the living organism can be obtained. The manufacturing process is simplified, and the manufacturing cost is reduced.

Also, when phytic acid is removed by releasing at least two phosphate groups from phytic acid,
A product can be obtained in which the absorption of minerals takes place more efficiently.

In each of the above embodiments, the case where the present invention is applied to soybean meal having protein as a main component has been described. However, the present invention utilizes rice or the like having starch as a main component as cereals. It is also possible to use a mixture of rice and soybean meal and soybean meal, and it is also possible to use a combination of other types of cereals. In addition, the present invention can be similarly applied to all products using phytic acid-containing grains as raw materials, that is, from human food to aquaculture feed and feed.

Further, in the present invention, it is possible to carry out the process using a conventional koji making apparatus as it is, and it is not necessary to particularly manufacture a production base apparatus, so that it is highly versatile.

Note that the present invention is not limited to the above embodiments, and can be modified as needed.

[0103]

As described above, the product of the present invention using the cereal as a raw material, the useful material for promoting the growth of microorganisms, and the method for producing the same are constructed and functioning. Promote the growth of useful microorganisms that can be obtained by using the product as a raw material and that have the effect of the product using the cereal as a raw material and that effectively act to maintain the health of the organism in breeding the organism. Can be done.

That is, the present inventors have proposed in Japanese Patent Application Laid-Open No. Hei 7-23725 that the product made from cereal as a raw material can be mass-produced more efficiently, and the coexistence and symbiosis with lactic acid bacteria and yeast contained in Aspergillus oryzae. A useful microbial growth-promoting material that can be obtained by using a cereal-based product that has already been proposed in Japanese Patent Application Laid-Open No. 7-23725 and that maintains the health of living organisms in breeding them. Therefore, a useful microorganism growth promoting material for promoting the growth of useful microorganisms that effectively act can be mass-produced more efficiently.

That is, according to the product using cereals as a raw material,
Phytic acid in cereals can be easily removed while keeping the state of the cereal in a solid state, and the activity of vitamin B and the like contained in the product is maintained at a high level. It is easy to absorb the contained minerals and the like, and it is also possible to obtain a product capable of promoting the absorption. The production process is simple and the production cost is low.

Furthermore, according to the useful microorganism growth promoting material,
When this useful microorganism growth promoting material is added to, for example, a food containing a useful microorganism, the growth of the useful microorganism is promoted, and various health promoting actions of the useful microorganism are exhibited, and the growth of harmful bacteria is surely prevented. Can be suppressed, the maintenance of the health of the organism that eats the food can be improved, and the growth of useful microorganisms that effectively act to maintain the health of the organism can be promoted in growing the organism. .

Further, when the useful microorganisms are naturally added to the product or separately added by hydrolysis during the subsequent hydrolysis of the product obtained by the completion of the koji making, the koji mold and the koji mold during the hydrolysis step The useful microorganism coexists and coexists with the product having the same substrate, the useful microorganism receives nutrients from the product to promote the growth, and the product is cultured with the koji mold and the useful microorganism together. Becomes And if this useful microorganism growth promoting material is added to food,
The useful microorganisms already cultured in the useful microorganism growth-promoting material and the useful microorganisms contained in the food are further promoted, and various health promoting actions of the useful microorganisms are exhibited, and the growth of harmful bacteria is performed. Can be reliably suppressed, and the maintenance of the health of the organism that eats the food can be improved.

Further, according to the present invention, the useful microorganism growth promoting material acting as described above can be manufactured with a simple manufacturing process and at a low manufacturing cost.

[Brief description of the drawings]

FIG. 1 is a process diagram showing one embodiment of a method for producing a product from which phytic acid in soybean meal has been removed according to the present invention.

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Claims (12)

[Claims] Claims 1. A cereal grain is inoculated with a koji mold to make koji, and a new cereal is added to a product obtained by the koji making treatment to obtain a mixed cereal.
A product using cereals as a raw material, wherein the cereals are produced by hydrolyzing proteins in the mixed cereals by adding water to the mixed cereals and removing phytic acid in the mixed cereals. 2. A cereal is added to the product cereal obtained from the cereal according to claim 1 to obtain an additional mixed cereal, and the protein in the additional mixed cereal is hydrolyzed by adding water to the additional mixed cereal. And a cereal-based product produced by removing phytic acid from the additional mixed cereal. 3. A cereal is inoculated with a koji mold to make koji, and a new cereal is added to a product obtained by the koji-making process to obtain a mixed cereal.
Promoting the growth of a useful microorganism characterized by being hydrolyzed into the mixed grain to hydrolyze proteins and / or carbohydrates in the mixed grain and removing a predetermined amount of phytic acid from the mixed grain. Useful microbial growth promoting material. 4. Useful microorganisms contained in the product of the koji making process and / or useful microorganisms added to the product of the koji making process are promoted to grow during the hydrolysis. The useful microorganism growth promoting material according to claim 3, characterized in that: 5. The useful microorganism growth promoting material according to claim 3, wherein the useful microorganism is at least one of a fungus, a lactic acid bacterium and a bifidobacterium. 6. An additional mixed cereal by adding a cereal to the useful microbial growth promoting material according to any one of claims 3 to 5, and adding the cereal to the additional mixed cereal by adding water thereto. A product made from cereals, which is produced by hydrolyzing proteins and / or carbohydrates and removing a predetermined amount of phytic acid from the additional mixed cereals. 7. A cereal is inoculated with a koji mold to make koji, and a new cereal is added to a product obtained by the koji-making treatment to form a mixed cereal.
By hydrolyzing the protein in the mixed cereal by adding water to the mixed cereal, the phytic acid in the mixed cereal is removed to produce a product using the cereal as a raw material. Production method for the product. 8. An additional mixed cereal by adding cereals to the product produced by the method for producing a product using cereals as a raw material according to claim 7, and adding the additional mixed cereals by adding water to the additional mixed cereals. A method for producing a cereal-based product, comprising hydrolyzing proteins in the cereal and removing phytic acid in the additional mixed cereal to produce a cereal-based product. . 9. A cereal is inoculated with a koji mold to produce koji, and a new cereal is added to a product obtained by the koji-making treatment to obtain a mixed cereal.
By hydrolyzing the mixed cereal, protein and / or carbohydrate in the mixed cereal is hydrolyzed, and a predetermined amount of phytic acid in the mixed cereal is removed to produce a useful microorganism growth promoting material for promoting useful microorganisms. Producing a useful microorganism growth promoting material. 10. The method according to claim 1, wherein in the hydrolysis step, useful microorganisms contained in the product of the koji making process and / or useful microorganisms added to the product of the koji making process are promoted. A method for producing a useful microorganism growth promoting material according to claim 9. 11. The method for producing a useful microorganism growth promoting material according to claim 9, wherein the useful microorganism is at least one of fungi, lactic acid bacteria and bifidobacteria. 12. The method according to claim 9, wherein
The cereal is further added to the useful microbial growth promoting material according to item to form an additional mixed cereal, and the protein and / or saccharide in the additional mixed cereal is hydrolyzed by adding water to the additional mixed cereal, and the additional mixed cereal is added to the additional mixed cereal. A method for producing a useful microorganism growth-promoting material, comprising: removing a predetermined amount of phytic acid to produce a useful microorganism growth-promoting material for promoting useful microorganism growth.