IT201800002411A1 - Domini strutturali di molecole di rna antisenso che aumentano la traduzione - Google Patents

Domini strutturali di molecole di rna antisenso che aumentano la traduzione Download PDF

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Publication number
IT201800002411A1
IT201800002411A1 IT201800002411A IT201800002411A IT201800002411A1 IT 201800002411 A1 IT201800002411 A1 IT 201800002411A1 IT 201800002411 A IT201800002411 A IT 201800002411A IT 201800002411 A IT201800002411 A IT 201800002411A IT 201800002411 A1 IT201800002411 A1 IT 201800002411A1
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IT
Italy
Prior art keywords
seq
sequence
sine
nucleic acid
domain
Prior art date
Application number
IT201800002411A
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English (en)
Italian (it)
Inventor
Stefano Gustincich
Silvia Zucchelli
Peter Podbevsek
Janez Plavec
Piero Carninci
Hazuki Takahashi
Toshio Yamazaki
Takako Ohyama
Harshita Sharma
Original Assignee
Scuola Int Superiore Di Studi Avanzati Sissa
Transsine Tech Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scuola Int Superiore Di Studi Avanzati Sissa, Transsine Tech Co Ltd filed Critical Scuola Int Superiore Di Studi Avanzati Sissa
Priority to IT201800002411A priority Critical patent/IT201800002411A1/it
Priority to KR1020207025572A priority patent/KR20210005548A/ko
Priority to EP19708167.2A priority patent/EP3749772A1/en
Priority to US16/967,254 priority patent/US12134768B2/en
Priority to CN201980023671.0A priority patent/CN112236524A/zh
Priority to PCT/IB2019/050914 priority patent/WO2019150346A1/en
Priority to JP2020563841A priority patent/JP2021512646A/ja
Priority to CA3090347A priority patent/CA3090347A1/en
Publication of IT201800002411A1 publication Critical patent/IT201800002411A1/it

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/532—Closed or circular
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/11—Applications; Uses in screening processes for the determination of target sites, i.e. of active nucleic acids

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
IT201800002411A 2018-02-05 2018-02-05 Domini strutturali di molecole di rna antisenso che aumentano la traduzione IT201800002411A1 (it)

Priority Applications (8)

Application Number Priority Date Filing Date Title
IT201800002411A IT201800002411A1 (it) 2018-02-05 2018-02-05 Domini strutturali di molecole di rna antisenso che aumentano la traduzione
KR1020207025572A KR20210005548A (ko) 2018-02-05 2019-02-05 번역을 상향-조절하는 안티센스 rna 분자의 구조 도메인
EP19708167.2A EP3749772A1 (en) 2018-02-05 2019-02-05 Structural domains of antisense rna molecules up-regulating translation
US16/967,254 US12134768B2 (en) 2018-02-05 2019-02-05 Structural domains of antisense RNA molecules up-regulating translation
CN201980023671.0A CN112236524A (zh) 2018-02-05 2019-02-05 上调翻译的反义rna分子的结构域
PCT/IB2019/050914 WO2019150346A1 (en) 2018-02-05 2019-02-05 Structural domains of antisense rna molecules up-regulating translation
JP2020563841A JP2021512646A (ja) 2018-02-05 2019-02-05 翻訳を上方調節するアンチセンスｒｎａ分子の構造ドメイン
CA3090347A CA3090347A1 (en) 2018-02-05 2019-02-05 Structural domains of antisense rna molecules up-regulating translation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT201800002411A IT201800002411A1 (it) 2018-02-05 2018-02-05 Domini strutturali di molecole di rna antisenso che aumentano la traduzione

Publications (1)

Publication Number Publication Date
IT201800002411A1 true IT201800002411A1 (it) 2019-08-05

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IT201800002411A IT201800002411A1 (it) 2018-02-05 2018-02-05 Domini strutturali di molecole di rna antisenso che aumentano la traduzione

Country Status (8)

Country Link
US (1) US12134768B2 (https=)
EP (1) EP3749772A1 (https=)
JP (1) JP2021512646A (https=)
KR (1) KR20210005548A (https=)
CN (1) CN112236524A (https=)
CA (1) CA3090347A1 (https=)
IT (1) IT201800002411A1 (https=)
WO (1) WO2019150346A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116322791A (zh) 2020-09-24 2023-06-23 意大利技术基金会 经修饰的功能性核酸分子
CA3196633A1 (en) * 2020-10-08 2022-04-14 Scuola Internazionale Superiore Di Studi Avanzati Functional nucleic acid molecules
EP3992289A1 (en) 2020-10-30 2022-05-04 Transine Therapeutics Limited Functional nucleic acid molecules incorporating protein binding domain
EP4063505A1 (en) 2021-03-22 2022-09-28 Fondazione Istituto Italiano di Tecnologia Functional nucleic acid molecules directed to targets for nervous system disorders
WO2022214635A1 (en) 2021-04-08 2022-10-13 Stichting Vu Nucleic acid molecules for compensation of stxbp1 haploinsufficiency and their use in the treatment of stxbp1-related disorders
GB202205423D0 (en) 2022-04-12 2022-05-25 Transine Therapeutics Ltd Functional nucleic acid molecule
GB202207796D0 (en) 2022-05-26 2022-07-13 Fondazione St Italiano Tecnologia Functional nucleic acid molecule
GB202207795D0 (en) * 2022-05-26 2022-07-13 Fondazione St Italiano Tecnologia Functional nucleic acid molecule
CN115054612B (zh) * 2022-06-15 2023-07-21 北京航空航天大学 一种可用于促进骨形成的增强子及其应用
CN119040324B (zh) * 2024-08-30 2025-06-24 广州国家实验室 一种tns1基因增强子及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012133947A1 (en) * 2011-03-30 2012-10-04 Riken Functional nucleic acid molecule and use thereof
WO2016070853A1 (zh) * 2014-11-04 2016-05-12 清华大学 含有反向sineb2重复序列的人工非编码rna及其在增强靶蛋白翻译中的用途

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102586237B (zh) * 2012-02-17 2017-12-22 北京大学 一种多聚核酸分子及其应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012133947A1 (en) * 2011-03-30 2012-10-04 Riken Functional nucleic acid molecule and use thereof
WO2016070853A1 (zh) * 2014-11-04 2016-05-12 清华大学 含有反向sineb2重复序列的人工非编码rna及其在增强靶蛋白翻译中的用途

Non-Patent Citations (5)

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Title
DEMOOR J M ET AL: "ANTISENSE NUCLEIC ACIDS TARGETED TO THE THYMIDYLATE SYNTHASE (TS) MRNA TRANSLATION START SITE STIMULATE TS GENE TRANSCRIPTION", EXPERIMENTAL CELL RESEARCH, ELSEVIER, AMSTERDAM, NL, vol. 243, 25 August 1998 (1998-08-25), pages 11 - 21, XP002923758, ISSN: 0014-4827, DOI: 10.1006/EXCR.1998.4059 *
GOSUKE HAYASHI ET AL: "Activation of prokaryotic translation by antisense oligonucleotides binding to coding region of mRNA", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 429, no. 1-2, 27 October 2012 (2012-10-27), AMSTERDAM, NL, pages 105 - 110, XP055265704, ISSN: 0006-291X, DOI: 10.1016/j.bbrc.2012.10.072 *
GOSUKE HAYASHI ET AL: "Supplementary Data: Activation of prokaryotic translation by antisense oligonucleotides binding to coding region of mRNA", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 27 October 2012 (2012-10-27), United States, pages 105 - 110, XP055483951, Retrieved from the Internet <URL:https://ars.els-cdn.com/content/image/1-s2.0-S0006291X1202044X-mmc1.doc> [retrieved on 20180613], DOI: 10.1016/j.bbrc.2012.10.072 *
PATRUCCO LAURA ET AL: "Engineering mammalian cell factories with SINEUP noncoding RNAs to improve translation of secreted proteins", GENE, ELSEVIER, AMSTERDAM, NL, vol. 569, no. 2, 2 June 2015 (2015-06-02), pages 287 - 293, XP029247971, ISSN: 0378-1119, DOI: 10.1016/J.GENE.2015.05.070 *
S ZUCCHELLI ET AL: "SINEUPs: A new class of natural and synthetic antisense long non-coding RNAs that activate translation", RNA BIOLOGY, vol. 12, no. 8, 3 August 2015 (2015-08-03), US, pages 771 - 779, XP055386212, ISSN: 1547-6286, DOI: 10.1080/15476286.2015.1060395 *

Also Published As

Publication number Publication date
US12134768B2 (en) 2024-11-05
CA3090347A1 (en) 2019-08-08
KR20210005548A (ko) 2021-01-14
CN112236524A (zh) 2021-01-15
JP2021512646A (ja) 2021-05-20
EP3749772A1 (en) 2020-12-16
US20210139898A1 (en) 2021-05-13
WO2019150346A1 (en) 2019-08-08

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