IN1998KO02070A - - Google Patents

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Publication number
IN1998KO02070A
IN1998KO02070A IN2070KO1998A IN1998KO02070A IN 1998KO02070 A IN1998KO02070 A IN 1998KO02070A IN 2070KO1998 A IN2070KO1998 A IN 2070KO1998A IN 1998KO02070 A IN1998KO02070 A IN 1998KO02070A
Authority
IN
India
Application number
Other languages
English (en)
Inventor
Johnathan A Napier
Original Assignee
Univ Bristol
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Bristol filed Critical Univ Bristol
Publication of IN1998KO02070A publication Critical patent/IN1998KO02070A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0083Miscellaneous (1.14.99)
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/16Drugs for disorders of the alimentary tract or the digestive system for liver or gallbladder disorders, e.g. hepatoprotective agents, cholagogues, litholytics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00Drugs for dermatological disorders
    • A61P17/10Anti-acne agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00Drugs for disorders of the metabolism
    • A61P3/08Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00Antineoplastic agents
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00Drugs for disorders of the cardiovascular system
    • A61P9/10Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00Drugs for disorders of the cardiovascular system
    • A61P9/12Antihypertensives
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6427Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Diabetes (AREA)
  • Nutrition Science (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Vascular Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Dermatology (AREA)
  • Rheumatology (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Pain & Pain Management (AREA)
IN2070KO1998 1997-11-24 1998-11-24 IN1998KO02070A (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9724783.7A GB9724783D0 (en) 1997-11-24 1997-11-24 Novel polypeptides

Publications (1)

Publication Number Publication Date
IN1998KO02070A true IN1998KO02070A (no) 2005-03-11

Family

ID=10822539

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2070KO1998 IN1998KO02070A (no) 1997-11-24 1998-11-24

Country Status (17)

Country Link
US (1) US6897050B1 (no)
EP (1) EP1032682A1 (no)
JP (1) JP2002505848A (no)
KR (1) KR20010032433A (no)
CN (1) CN1285874A (no)
AU (1) AU762750B2 (no)
BR (1) BR9815419A (no)
CA (1) CA2311472A1 (no)
EE (1) EE200000304A (no)
GB (1) GB9724783D0 (no)
HU (1) HUP0004484A3 (no)
IN (1) IN1998KO02070A (no)
MX (1) MXPA00005109A (no)
PL (1) PL340760A1 (no)
RU (1) RU2248397C2 (no)
WO (1) WO1999027111A1 (no)
ZA (1) ZA9810716B (no)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
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US6355861B1 (en) * 1991-10-10 2002-03-12 Rhone-Poulenc Agrochimie Production of gamma linolenic acid by a Δ6-desaturase
US6683232B1 (en) 1991-10-10 2004-01-27 Rhone-Poulenc Agrochimie Production of γ linolenic acid by a Δ6-desaturase
AU776447B2 (en) 1999-06-07 2004-09-09 Basf Plant Science Gmbh Delta6-acetylenase and delta6-desaturase from ceratodon purpureus
DE10030976A1 (de) 2000-06-30 2002-01-10 Basf Ag DELTA6-Desaturasegene exprimierende Pflanzen und PUFAS enthaltende Öle aus diesen Pflanzen und ein Verfahren zur Herstellung ungesättigter Fettsäuren
WO2001002591A1 (de) * 1999-07-06 2001-01-11 Basf Plant Science Gmbh Δ6-desaturasegene exprimierende pflanzen und pufas enthaltende öle aus diesen pflanzen und ein verfahren zur herstellung ungesättigter fettsäuren
DK1322752T3 (da) 2000-09-28 2010-07-19 Bioriginal Food & Science Corp FAD4, FAD5, FAD5-2, og FAD6-medlemmer af fedtsyredesaturasefamilien og anvendelser deraf
DE10102337A1 (de) 2001-01-19 2002-07-25 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren, neue Biosynthesegene sowie neue pflanzliche Expressionskonstrukte
CN1625597A (zh) 2002-01-30 2005-06-08 巴斯福植物科学有限公司 新的延长酶基因及多不饱和脂肪酸的制备方法
GB2385852A (en) 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
DE10219203A1 (de) 2002-04-29 2003-11-13 Basf Plant Science Gmbh Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in Pflanzen
AU2003296638B2 (en) 2002-12-19 2009-06-11 University Of Bristol Method for the production of polyunsaturated fatty acids
AU2004215705B2 (en) 2003-02-27 2009-11-12 Basf Plant Science Gmbh Method for the production of polyunsaturated fatty acids
EP2390313B1 (de) 2003-03-31 2016-05-11 University Of Bristol Neue pflanzliche Acyltransferase spezifisch für langkettige mehrfach ungesättigte Fettsäuren
MXPA05010571A (es) 2003-04-08 2005-11-23 Basf Plant Science Gmbh Delta-4-desaturasas a partir de euglena gracilis, que expresan plantas y aceites que comprenden pufa
EP2172536B1 (de) 2003-08-01 2016-05-04 BASF Plant Science GmbH Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen
US11952581B2 (en) 2003-08-01 2024-04-09 Basf Plant Science Gmbh Process for the production of polyunsaturated fatty acids in transgenic organisms
EP1720988B1 (de) 2004-02-27 2011-10-12 BASF Plant Science GmbH Verfahren zur herstellung von ungesättigten omega-3-fettsäuren in transgenen organismen
CN1930277B (zh) 2004-02-27 2011-02-02 巴斯福植物科学有限公司 在转基因植物中产生多不饱和脂肪酸的方法
PL1756280T3 (pl) 2004-04-22 2015-04-30 Commw Scient Ind Res Org Synteza długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach rekombinowanych
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
PL1766023T3 (pl) 2004-06-04 2011-04-29 Fluxome Sciences As Metabolicznie zmodyfikowane komórki do wytwarzania wielonienasyconych kwasów tłuszczowych
DE102005013779A1 (de) 2005-03-22 2006-09-28 Basf Plant Science Gmbh Verfahren zur Herstellung von mehrfach ungesättigten C20- und C22-Fettsäuren mit mindestens vier Doppelbindungen in transgenen Pflanzen
DE102005038036A1 (de) 2005-08-09 2007-02-15 Basf Plant Science Gmbh Verfahren zur Herstellung von Arachidonsäure und/oder Eicosapentaensäure in transgenen Nutzpflanzen
GB2431158A (en) 2005-10-13 2007-04-18 Rothamsted Res Ltd Process for the production of arachidonic and/or eicosapentaenoic acid
DE102005052551A1 (de) 2005-11-02 2007-05-16 Rothamsted Res Harpenden Verfahren zur Herstellung von y-Linolensäure und/oder Stearidonsäure in transgenen Brassicaceae und Linaceae
GB0603160D0 (en) 2006-02-16 2006-03-29 Rothamsted Res Ltd Nucleic acid
AR059376A1 (es) 2006-02-21 2008-03-26 Basf Plant Science Gmbh Procedimiento para la produccion de acidos grasos poliinsaturados
US8629195B2 (en) 2006-04-08 2014-01-14 Bayer Materialscience Ag Production of polyurethane foams
AU2007287585B2 (en) 2006-08-24 2013-08-29 Basf Plant Science Gmbh Isolation and characterization of a novel Pythium omega 3 desaturase with specificity to all omega 6 fatty acids longer than 18 carbon chains
RU2009111266A (ru) 2006-08-29 2010-10-10 Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) Синтез жирных кислот
WO2008040787A2 (de) 2006-10-06 2008-04-10 Basf Plant Science Gmbh Verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen organismen
EP2176416B1 (de) 2007-07-31 2016-03-16 BASF Plant Science GmbH Elongasen und verfahren zur herstellung mehrfach ungesättigter fettsäuren in transgenen organismen
US20100263088A1 (en) 2007-12-14 2010-10-14 Basf Plant Science Gmbh Promoters From Brassica Napus For Seed Specific Gene Expression
CA2723072C (en) 2008-04-30 2017-01-10 Rothamsted Research Ltd. Desaturase and method for the production of polyunsaturated fatty acids in transgenic organisms
AU2009265795B2 (en) 2008-07-01 2014-12-11 Basf Plant Science Gmbh Promoters from Brassica napus for seed specific gene expression
JP5757870B2 (ja) 2008-08-26 2015-08-05 ビーエーエスエフ プラント サイエンス ゲーエムベーハー デサチュラーゼをコードする核酸および改変植物油
CN102317459A (zh) 2008-11-18 2012-01-11 联邦科学技术研究组织 产生ω-3脂肪酸的酶和方法
PL2376638T3 (pl) 2008-12-12 2014-01-31 Basf Plant Science Gmbh Desaturazy oraz sposób wytwarzania wielonienasyconych kwasów tłuszczowych w organizmach transgenicznych
EP2821492A3 (en) 2009-05-13 2015-04-08 BASF Plant Science Company GmbH Acyltransferases and uses thereof in fatty acid production
CA2765560C (en) * 2009-06-22 2017-10-31 Saga University Mutation that increases the oleic acid content in soybean oil and responsible gene thereof
AU2011269059B2 (en) 2010-06-25 2016-01-28 Basf Plant Science Company Gmbh Acyltransferases and uses therof in fatty acid production
WO2013153404A1 (en) 2012-04-12 2013-10-17 Rothamsted Research Ltd Production of omega-3 long chain polyunsaturated fatty acids
NZ631702A (en) 2012-06-15 2017-01-27 Grains Res & Dev Corp Production of long chain polyunsaturated fatty acids in plant cells
GB201217524D0 (en) 2012-10-01 2012-11-14 Rothamsted Res Ltd Recombinant organisms
US9725399B2 (en) 2013-12-18 2017-08-08 Commonwealth Scientific And Industrial Research Organisation Lipid comprising long chain polyunsaturated fatty acids
KR102527795B1 (ko) 2014-06-27 2023-05-02 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 도코사펜타에노산을 포함하는 지질

Family Cites Families (6)

* Cited by examiner, † Cited by third party
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GB8813766D0 (en) 1988-06-10 1988-07-13 Efamol Holdings Essential fatty acid compositions
EP0400547A1 (en) * 1989-06-02 1990-12-05 Abbott Laboratories Parenteral nutrition product
IE892333A1 (en) * 1989-07-19 1991-06-19 Univ Galway College Diagnostic assays and antibodies for use in said assays
US5614393A (en) * 1991-10-10 1997-03-25 Rhone-Poulenc Agrochimie Production of γ-linolenic acid by a Δ6-desaturase
PT1598422E (pt) * 1997-04-11 2013-04-03 Abbott Lab Métodos e composições para síntese de ácidos gordos poliinsaturados de cadeia longa em plantas
US5968809A (en) * 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids

Also Published As

Publication number Publication date
PL340760A1 (en) 2001-02-26
WO1999027111A1 (en) 1999-06-03
GB9724783D0 (en) 1998-01-21
AU762750B2 (en) 2003-07-03
BR9815419A (pt) 2007-05-22
EP1032682A1 (en) 2000-09-06
EE200000304A (et) 2001-06-15
US6897050B1 (en) 2005-05-24
CA2311472A1 (en) 1999-06-03
HUP0004484A2 (hu) 2001-04-28
MXPA00005109A (es) 2001-12-31
AU1249799A (en) 1999-06-15
JP2002505848A (ja) 2002-02-26
ZA9810716B (en) 1999-06-16
RU2248397C2 (ru) 2005-03-20
KR20010032433A (ko) 2001-04-25
CN1285874A (zh) 2001-02-28
HUP0004484A3 (en) 2002-11-28

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