IL95176A - Increased activity of naphthalene dioxinase during the production process of indigo microbial - Google Patents
Increased activity of naphthalene dioxinase during the production process of indigo microbialInfo
- Publication number
- IL95176A IL95176A IL9517690A IL9517690A IL95176A IL 95176 A IL95176 A IL 95176A IL 9517690 A IL9517690 A IL 9517690A IL 9517690 A IL9517690 A IL 9517690A IL 95176 A IL95176 A IL 95176A
- Authority
- IL
- Israel
- Prior art keywords
- naphthalene dioxygenase
- naphthalene
- ferredoxin
- iron
- plasmid
- Prior art date
Links
- 230000000694 effects Effects 0.000 title claims abstract description 59
- 108010041042 naphthalene dioxygenase Proteins 0.000 title claims abstract description 54
- COHYTHOBJLSHDF-UHFFFAOYSA-N indigo powder Natural products N1C2=CC=CC=C2C(=O)C1=C1C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-UHFFFAOYSA-N 0.000 title claims abstract description 25
- 235000000177 Indigofera tinctoria Nutrition 0.000 title claims abstract description 24
- 229940097275 indigo Drugs 0.000 title claims abstract description 24
- 238000004519 manufacturing process Methods 0.000 title description 10
- 230000000813 microbial effect Effects 0.000 title description 5
- 229920001184 polypeptide Polymers 0.000 claims abstract description 54
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 54
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 54
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 48
- 108010074122 Ferredoxins Proteins 0.000 claims abstract description 46
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 44
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 claims abstract description 42
- 108010081409 Iron-Sulfur Proteins Proteins 0.000 claims abstract description 25
- 102000005298 Iron-Sulfur Proteins Human genes 0.000 claims abstract description 25
- 229910052742 iron Inorganic materials 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 24
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 claims abstract description 21
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 claims abstract description 21
- 230000008569 process Effects 0.000 claims abstract description 20
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical compound [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 claims abstract description 19
- 102000004316 Oxidoreductases Human genes 0.000 claims abstract description 18
- 108090000854 Oxidoreductases Proteins 0.000 claims abstract description 18
- 230000001131 transforming effect Effects 0.000 claims abstract description 13
- 239000013612 plasmid Substances 0.000 claims description 85
- UFWIBTONFRDIAS-UHFFFAOYSA-N Naphthalene Chemical compound C1=CC=CC2=CC=CC=C21 UFWIBTONFRDIAS-UHFFFAOYSA-N 0.000 claims description 18
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 14
- 244000005700 microbiome Species 0.000 claims description 13
- 230000002708 enhancing effect Effects 0.000 claims description 9
- 241000589776 Pseudomonas putida Species 0.000 claims description 4
- 108010028143 Dioxygenases Proteins 0.000 claims 2
- 102000016680 Dioxygenases Human genes 0.000 claims 2
- 108020004414 DNA Proteins 0.000 abstract description 17
- 108090000623 proteins and genes Proteins 0.000 description 34
- 239000000284 extract Substances 0.000 description 22
- 239000012634 fragment Substances 0.000 description 18
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 11
- 102000004190 Enzymes Human genes 0.000 description 10
- 108090000790 Enzymes Proteins 0.000 description 10
- 229940075566 naphthalene Drugs 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 8
- PCKPVGOLPKLUHR-UHFFFAOYSA-N indoxyl Chemical group C1=CC=C2C(O)=CNC2=C1 PCKPVGOLPKLUHR-UHFFFAOYSA-N 0.000 description 8
- 238000010276 construction Methods 0.000 description 7
- 241000588724 Escherichia coli Species 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 150000001413 amino acids Chemical class 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- XVARCVCWNFACQC-RKQHYHRCSA-N indican Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=CC=C12 XVARCVCWNFACQC-RKQHYHRCSA-N 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 101150073444 rd gene Proteins 0.000 description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 3
- 101100177572 Porphyromonas gingivalis (strain ATCC BAA-308 / W83) nahA gene Proteins 0.000 description 3
- 239000000287 crude extract Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000002741 site-directed mutagenesis Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108091081024 Start codon Proteins 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000007621 bhi medium Substances 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- BXFFHSIDQOFMLE-UHFFFAOYSA-N indoxyl sulfate Natural products C1=CC=C2C(OS(=O)(=O)O)=CNC2=C1 BXFFHSIDQOFMLE-UHFFFAOYSA-N 0.000 description 2
- XVARCVCWNFACQC-UHFFFAOYSA-N indoxyl-beta-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1=CNC2=CC=CC=C12 XVARCVCWNFACQC-UHFFFAOYSA-N 0.000 description 2
- 101150066764 isp gene Proteins 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 101150102798 nahB gene Proteins 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- QPUHWUSUBHNZCG-VHSXEESVSA-N (1R,2S)-1,2-dihydronaphthalene-1,2-diol Chemical compound C1=CC=C2[C@@H](O)[C@@H](O)C=CC2=C1 QPUHWUSUBHNZCG-VHSXEESVSA-N 0.000 description 1
- MLCFNGQWWKZFBD-HTQZYQBOSA-N (2r,3r)-2,3-dihydro-1h-indole-2,3-diol Chemical compound C1=CC=C2[C@@H](O)[C@@H](O)NC2=C1 MLCFNGQWWKZFBD-HTQZYQBOSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- NIKBMHGRNAPJFW-IUCAKERBSA-N His-Arg Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 NIKBMHGRNAPJFW-IUCAKERBSA-N 0.000 description 1
- MAJYPBAJPNUFPV-BQBZGAKWSA-N His-Cys Chemical compound SC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 MAJYPBAJPNUFPV-BQBZGAKWSA-N 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 102000004020 Oxygenases Human genes 0.000 description 1
- 108090000417 Oxygenases Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 108020005038 Terminator Codon Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000001055 blue pigment Substances 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000004177 carbon cycle Methods 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 108010051678 cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase Proteins 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000007429 general method Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- COHYTHOBJLSHDF-BUHFOSPRSA-N indigo dye Chemical compound N\1C2=CC=CC=C2C(=O)C/1=C1/C(=O)C2=CC=CC=C2N1 COHYTHOBJLSHDF-BUHFOSPRSA-N 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 239000000543 intermediate Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 238000005567 liquid scintillation counting Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000007269 microbial metabolism Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 101150018284 nahA gene Proteins 0.000 description 1
- 150000002790 naphthalenes Chemical class 0.000 description 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000010076 replication Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 125000004354 sulfur functional group Chemical group 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/16—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing two or more hetero rings
- C12P17/165—Heterorings having nitrogen atoms as the only ring heteroatoms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
- C12Y114/12—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of two atoms of oxygen into one donor (1.14.12)
- C12Y114/12012—Naphthalene 1,2-dioxygenase (1.14.12.12)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US38973889A | 1989-08-04 | 1989-08-04 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IL95176A0 IL95176A0 (en) | 1991-06-10 |
| IL95176A true IL95176A (en) | 1999-01-26 |
Family
ID=23539526
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL9517690A IL95176A (en) | 1989-08-04 | 1990-07-24 | Increased activity of naphthalene dioxinase during the production process of indigo microbial |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0437579B1 (de) |
| JP (1) | JP2974407B2 (de) |
| AT (1) | ATE200693T1 (de) |
| CA (1) | CA2022285C (de) |
| DE (1) | DE69033725T2 (de) |
| IE (1) | IE902821A1 (de) |
| IL (1) | IL95176A (de) |
| SG (1) | SG48998A1 (de) |
| WO (1) | WO1991002055A1 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5496715A (en) * | 1993-08-25 | 1996-03-05 | Mitsubishi Chemical Corporation | Process for preparing indigo |
| CN1317388C (zh) * | 2003-11-28 | 2007-05-23 | 中国科学院微生物研究所 | 2－氨基酚1，6－双加氧酶、其基因及用途 |
| CN101955951B (zh) * | 2009-07-17 | 2014-06-25 | 上海市农业科学院 | 不动杆菌萘双加氧酶系统基因及其应用 |
| CN116769806A (zh) * | 2023-05-06 | 2023-09-19 | 上海市农业科学院 | 萘双加氧酶基因簇、过表达载体及其重组菌株在降解有机污染物中的应用 |
-
1990
- 1990-07-19 WO PCT/US1990/004128 patent/WO1991002055A1/en not_active Ceased
- 1990-07-19 JP JP2511626A patent/JP2974407B2/ja not_active Expired - Lifetime
- 1990-07-19 AT AT90912129T patent/ATE200693T1/de not_active IP Right Cessation
- 1990-07-19 EP EP90912129A patent/EP0437579B1/de not_active Expired - Lifetime
- 1990-07-19 DE DE69033725T patent/DE69033725T2/de not_active Expired - Lifetime
- 1990-07-24 IL IL9517690A patent/IL95176A/en not_active IP Right Cessation
- 1990-07-30 CA CA002022285A patent/CA2022285C/en not_active Expired - Lifetime
- 1990-08-03 IE IE282190A patent/IE902821A1/en unknown
- 1990-12-19 SG SG1996005037A patent/SG48998A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0437579A4 (en) | 1992-08-05 |
| ATE200693T1 (de) | 2001-05-15 |
| IE902821A1 (en) | 1991-02-27 |
| DE69033725D1 (de) | 2001-05-23 |
| EP0437579B1 (de) | 2001-04-18 |
| EP0437579A1 (de) | 1991-07-24 |
| WO1991002055A1 (en) | 1991-02-21 |
| CA2022285A1 (en) | 1991-02-05 |
| JP2974407B2 (ja) | 1999-11-10 |
| IL95176A0 (en) | 1991-06-10 |
| DE69033725T2 (de) | 2001-08-09 |
| CA2022285C (en) | 2002-12-31 |
| SG48998A1 (en) | 1998-05-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| RH | Patent void |