IL280817B2 - Synthesis of dna with improved yield - Google Patents
Synthesis of dna with improved yieldInfo
- Publication number
- IL280817B2 IL280817B2 IL280817A IL28081721A IL280817B2 IL 280817 B2 IL280817 B2 IL 280817B2 IL 280817 A IL280817 A IL 280817A IL 28081721 A IL28081721 A IL 28081721A IL 280817 B2 IL280817 B2 IL 280817B2
- Authority
- IL
- Israel
- Prior art keywords
- dna
- salts
- cell
- nucleotide
- free process
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/125—Specific component of sample, medium or buffer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/137—Concentration of a component of medium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/125—Rolling circle
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1813429.6A GB201813429D0 (en) | 2018-08-17 | 2018-08-17 | Synthesis of DNA with improved yield |
| PCT/GB2019/052307 WO2020035698A1 (en) | 2018-08-17 | 2019-08-16 | Synthesis of dna with improved yield |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| IL280817A IL280817A (en) | 2021-04-29 |
| IL280817B1 IL280817B1 (en) | 2025-04-01 |
| IL280817B2 true IL280817B2 (en) | 2025-08-01 |
Family
ID=63668172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL280817A IL280817B2 (en) | 2018-08-17 | 2019-08-16 | Synthesis of dna with improved yield |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20210301312A1 (https=) |
| EP (1) | EP3837382A1 (https=) |
| JP (2) | JP7548581B2 (https=) |
| KR (1) | KR20210084431A (https=) |
| CN (1) | CN113056565A (https=) |
| AU (1) | AU2019321778B2 (https=) |
| BR (1) | BR112021002764A2 (https=) |
| CA (1) | CA3109755A1 (https=) |
| GB (1) | GB201813429D0 (https=) |
| IL (1) | IL280817B2 (https=) |
| MX (1) | MX2021001729A (https=) |
| SG (1) | SG11202101548YA (https=) |
| WO (1) | WO2020035698A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB202002068D0 (en) * | 2020-02-14 | 2020-04-01 | Touchlight Ip Ltd | DNA synthesis yield improvements |
| GB202111742D0 (en) | 2021-08-16 | 2021-09-29 | Touchlight Ip Ltd | Improved process |
| WO2023069948A1 (en) | 2021-10-18 | 2023-04-27 | Flagship Pioneering Innovations Vii, Llc | Dna compositions and related methods |
| GB202319998D0 (en) | 2023-12-22 | 2024-02-07 | Touchlight Ip Ltd | duplexing method |
| EP4729632A1 (en) | 2024-10-18 | 2026-04-22 | AskBio Inc. | Method of synthesizing dna |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016034849A1 (en) * | 2014-09-05 | 2016-03-10 | Touchlight Genetics Limited | Synthesis of dna |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8435774B2 (en) * | 2006-06-28 | 2013-05-07 | Qiagen Gmbh | Enhancing reactivation of thermostable reversibly inactivated enzymes |
| US8986930B2 (en) * | 2010-07-12 | 2015-03-24 | Pacific Biosciences Of California, Inc. | Sequencing reactions with alkali metal cations for pulse width control |
| CN103608467B (zh) * | 2011-04-20 | 2017-07-21 | 美飒生物技术公司 | 用于核酸的振荡扩增反应 |
| WO2013101783A2 (en) * | 2011-12-30 | 2013-07-04 | Bio-Rad Laboratories, Inc. | Methods and compositions for performing nucleic acid amplification reactions |
| US9587263B2 (en) * | 2014-03-26 | 2017-03-07 | General Electric Company | Isothermal amplification under low salt condition |
-
2018
- 2018-08-17 GB GBGB1813429.6A patent/GB201813429D0/en not_active Ceased
-
2019
- 2019-08-16 IL IL280817A patent/IL280817B2/en unknown
- 2019-08-16 JP JP2021507969A patent/JP7548581B2/ja active Active
- 2019-08-16 EP EP19755959.4A patent/EP3837382A1/en active Pending
- 2019-08-16 CN CN201980067811.4A patent/CN113056565A/zh active Pending
- 2019-08-16 WO PCT/GB2019/052307 patent/WO2020035698A1/en not_active Ceased
- 2019-08-16 CA CA3109755A patent/CA3109755A1/en active Pending
- 2019-08-16 AU AU2019321778A patent/AU2019321778B2/en active Active
- 2019-08-16 US US17/268,721 patent/US20210301312A1/en not_active Abandoned
- 2019-08-16 KR KR1020217007998A patent/KR20210084431A/ko active Pending
- 2019-08-16 BR BR112021002764-5A patent/BR112021002764A2/pt unknown
- 2019-08-16 SG SG11202101548YA patent/SG11202101548YA/en unknown
- 2019-08-16 MX MX2021001729A patent/MX2021001729A/es unknown
-
2024
- 2024-05-02 JP JP2024074647A patent/JP7812580B2/ja active Active
-
2025
- 2025-06-10 US US19/233,542 patent/US20260028659A1/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016034849A1 (en) * | 2014-09-05 | 2016-03-10 | Touchlight Genetics Limited | Synthesis of dna |
Non-Patent Citations (2)
| Title |
|---|
| KLENOW, H., & HENNINGSEN, I., EFFECT OF MONOVALENT CATIONS ON THE ACTIVITY OF THE DNA POLYMERASE OF ESCHERICHIA COLI B, 1 May 1969 (1969-05-01) * |
| WALWICK, E. R., & MAIN, R. K., MONOVALENT CATION EFFECTS ON A DEOXYRIBONUCLEIC ACID-SYNTHESIZING SYSTEM, 1 January 1962 (1962-01-01) * |
Also Published As
| Publication number | Publication date |
|---|---|
| SG11202101548YA (en) | 2021-03-30 |
| MX2021001729A (es) | 2021-04-19 |
| JP7548581B2 (ja) | 2024-09-10 |
| AU2019321778A1 (en) | 2021-02-25 |
| JP2021534746A (ja) | 2021-12-16 |
| KR20210084431A (ko) | 2021-07-07 |
| JP2024099814A (ja) | 2024-07-25 |
| AU2019321778B2 (en) | 2025-05-08 |
| EP3837382A1 (en) | 2021-06-23 |
| CN113056565A (zh) | 2021-06-29 |
| GB201813429D0 (en) | 2018-10-03 |
| US20260028659A1 (en) | 2026-01-29 |
| US20210301312A1 (en) | 2021-09-30 |
| IL280817B1 (en) | 2025-04-01 |
| JP7812580B2 (ja) | 2026-02-10 |
| WO2020035698A1 (en) | 2020-02-20 |
| BR112021002764A2 (pt) | 2021-07-20 |
| IL280817A (en) | 2021-04-29 |
| CA3109755A1 (en) | 2020-02-20 |
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