AU2019321778B2 - Synthesis of DNA with improved yield - Google Patents

Synthesis of DNA with improved yield Download PDF

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Publication number
AU2019321778B2
AU2019321778B2 AU2019321778A AU2019321778A AU2019321778B2 AU 2019321778 B2 AU2019321778 B2 AU 2019321778B2 AU 2019321778 A AU2019321778 A AU 2019321778A AU 2019321778 A AU2019321778 A AU 2019321778A AU 2019321778 B2 AU2019321778 B2 AU 2019321778B2
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AU
Australia
Prior art keywords
dna
dntps
cell
salts
nucleotide
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AU2019321778A
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English (en)
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AU2019321778A1 (en
Inventor
Thomas Antony James ADIE
Anna BABULA
Stefanie GEHRIG-GIANNINI
Neil Porter
Paul James ROTHWELL
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Touchlight IP Ltd
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Touchlight IP Ltd
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Publication of AU2019321778A1 publication Critical patent/AU2019321778A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
AU2019321778A 2018-08-17 2019-08-16 Synthesis of DNA with improved yield Active AU2019321778B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1813429.6 2018-08-17
GBGB1813429.6A GB201813429D0 (en) 2018-08-17 2018-08-17 Synthesis of DNA with improved yield
PCT/GB2019/052307 WO2020035698A1 (en) 2018-08-17 2019-08-16 Synthesis of dna with improved yield

Publications (2)

Publication Number Publication Date
AU2019321778A1 AU2019321778A1 (en) 2021-02-25
AU2019321778B2 true AU2019321778B2 (en) 2025-05-08

Family

ID=63668172

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2019321778A Active AU2019321778B2 (en) 2018-08-17 2019-08-16 Synthesis of DNA with improved yield

Country Status (13)

Country Link
US (2) US20210301312A1 (https=)
EP (1) EP3837382A1 (https=)
JP (2) JP7548581B2 (https=)
KR (1) KR20210084431A (https=)
CN (1) CN113056565A (https=)
AU (1) AU2019321778B2 (https=)
BR (1) BR112021002764A2 (https=)
CA (1) CA3109755A1 (https=)
GB (1) GB201813429D0 (https=)
IL (1) IL280817B2 (https=)
MX (1) MX2021001729A (https=)
SG (1) SG11202101548YA (https=)
WO (1) WO2020035698A1 (https=)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202002068D0 (en) * 2020-02-14 2020-04-01 Touchlight Ip Ltd DNA synthesis yield improvements
GB202111742D0 (en) 2021-08-16 2021-09-29 Touchlight Ip Ltd Improved process
WO2023069948A1 (en) 2021-10-18 2023-04-27 Flagship Pioneering Innovations Vii, Llc Dna compositions and related methods
GB202319998D0 (en) 2023-12-22 2024-02-07 Touchlight Ip Ltd duplexing method
EP4729632A1 (en) 2024-10-18 2026-04-22 AskBio Inc. Method of synthesizing dna

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120009567A1 (en) * 2010-07-12 2012-01-12 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8435774B2 (en) * 2006-06-28 2013-05-07 Qiagen Gmbh Enhancing reactivation of thermostable reversibly inactivated enzymes
CN103608467B (zh) * 2011-04-20 2017-07-21 美飒生物技术公司 用于核酸的振荡扩增反应
WO2013101783A2 (en) * 2011-12-30 2013-07-04 Bio-Rad Laboratories, Inc. Methods and compositions for performing nucleic acid amplification reactions
US9587263B2 (en) * 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
GB201415789D0 (en) 2014-09-05 2014-10-22 Touchlight Genetics Ltd Synthesis of DNA

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120009567A1 (en) * 2010-07-12 2012-01-12 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
E.R WALWICK ET AL: "Monovalent cation effects on a deoxyribonucleic acid-synthesizing system", BBA SPECIALIZED SECTION ON NUCLEIC ACIDS AND RELATED SUBJECTS, 1 January 1962 (1962-01-01), pages 876 - 884, , DOI: 10.1016/0926-6550(62)90004-X *
KLENOW H ET AL: "Effect of monovalent cations on the activity of the DNA polymerase of Escherichia coli B.", EUROPEAN JOURNAL OF BIOCHEMISTRY 01 MAY 1969, vol. 9, no. 1, 1 May 1969 (1969-05-01), pages 133 - 141, *

Also Published As

Publication number Publication date
SG11202101548YA (en) 2021-03-30
IL280817B2 (en) 2025-08-01
MX2021001729A (es) 2021-04-19
JP7548581B2 (ja) 2024-09-10
AU2019321778A1 (en) 2021-02-25
JP2021534746A (ja) 2021-12-16
KR20210084431A (ko) 2021-07-07
JP2024099814A (ja) 2024-07-25
EP3837382A1 (en) 2021-06-23
CN113056565A (zh) 2021-06-29
GB201813429D0 (en) 2018-10-03
US20260028659A1 (en) 2026-01-29
US20210301312A1 (en) 2021-09-30
IL280817B1 (en) 2025-04-01
JP7812580B2 (ja) 2026-02-10
WO2020035698A1 (en) 2020-02-20
BR112021002764A2 (pt) 2021-07-20
IL280817A (en) 2021-04-29
CA3109755A1 (en) 2020-02-20

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