MX2021001729A - Sintesis de adn con rendimiento mejorado. - Google Patents

Sintesis de adn con rendimiento mejorado.

Info

Publication number
MX2021001729A
MX2021001729A MX2021001729A MX2021001729A MX2021001729A MX 2021001729 A MX2021001729 A MX 2021001729A MX 2021001729 A MX2021001729 A MX 2021001729A MX 2021001729 A MX2021001729 A MX 2021001729A MX 2021001729 A MX2021001729 A MX 2021001729A
Authority
MX
Mexico
Prior art keywords
dna
synthesis
improved
yield
nucleotides
Prior art date
Application number
MX2021001729A
Other languages
English (en)
Inventor
Neil Porter
Paul James Rothwell
Stefanie Gehrig-Giannini
Anna Babula
Thomas Antony James Adie
Original Assignee
Touchlight Ip Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Touchlight Ip Ltd filed Critical Touchlight Ip Ltd
Publication of MX2021001729A publication Critical patent/MX2021001729A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/125—Rolling circle

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

La presente invención se refiere a un proceso mejorado para la síntesis de ácido desoxirribonucleico (ADN), en particular una síntesis enzimática de ADN libre de células, preferiblemente a gran escala, con un rendimiento mejorado y/o con una eficiencia mejorada. La especie de catión presente en la sal de nucleótido como el contraión es crucial para el rendimiento, eficiencia y fidelidad de la reacción de síntesis enzimática de ADN de alto rendimiento. Los procesos descritos en este documento usan cationes alternativos como contraiones para los nucleótidos iónicos, lo que permite el uso de concentraciones más altas de nucleótidos en la síntesis de ADN, y permite además que se usen condiciones de reacción más favorables.
MX2021001729A 2018-08-17 2019-08-16 Sintesis de adn con rendimiento mejorado. MX2021001729A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1813429.6A GB201813429D0 (en) 2018-08-17 2018-08-17 Synthesis of DNA with improved yield
PCT/GB2019/052307 WO2020035698A1 (en) 2018-08-17 2019-08-16 Synthesis of dna with improved yield

Publications (1)

Publication Number Publication Date
MX2021001729A true MX2021001729A (es) 2021-04-19

Family

ID=63668172

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021001729A MX2021001729A (es) 2018-08-17 2019-08-16 Sintesis de adn con rendimiento mejorado.

Country Status (13)

Country Link
US (1) US20210301312A1 (es)
EP (1) EP3837382A1 (es)
JP (2) JP7548581B2 (es)
KR (1) KR20210084431A (es)
CN (1) CN113056565A (es)
AU (1) AU2019321778A1 (es)
BR (1) BR112021002764A2 (es)
CA (1) CA3109755A1 (es)
GB (1) GB201813429D0 (es)
IL (1) IL280817A (es)
MX (1) MX2021001729A (es)
SG (1) SG11202101548YA (es)
WO (1) WO2020035698A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202002068D0 (en) * 2020-02-14 2020-04-01 Touchlight Ip Ltd DNA synthesis yield improvements
GB202111742D0 (en) 2021-08-16 2021-09-29 Touchlight Ip Ltd Improved process

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8435774B2 (en) * 2006-06-28 2013-05-07 Qiagen Gmbh Enhancing reactivation of thermostable reversibly inactivated enzymes
US8986930B2 (en) * 2010-07-12 2015-03-24 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US9428781B2 (en) * 2011-04-20 2016-08-30 Mesa Biotech, Inc. Oscillating amplification reaction for nucleic acids
US20130217071A1 (en) * 2011-12-30 2013-08-22 Luz Montesclaros Methods and compositions for performing nucleic acid amplification reactions
US9587263B2 (en) * 2014-03-26 2017-03-07 General Electric Company Isothermal amplification under low salt condition
GB201415789D0 (en) * 2014-09-05 2014-10-22 Touchlight Genetics Ltd Synthesis of DNA

Also Published As

Publication number Publication date
BR112021002764A2 (pt) 2021-07-20
GB201813429D0 (en) 2018-10-03
CA3109755A1 (en) 2020-02-20
JP7548581B2 (ja) 2024-09-10
AU2019321778A1 (en) 2021-02-25
US20210301312A1 (en) 2021-09-30
EP3837382A1 (en) 2021-06-23
JP2024099814A (ja) 2024-07-25
IL280817A (en) 2021-04-29
KR20210084431A (ko) 2021-07-07
WO2020035698A1 (en) 2020-02-20
JP2021534746A (ja) 2021-12-16
CN113056565A (zh) 2021-06-29
SG11202101548YA (en) 2021-03-30

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