IL276781B1 - Methods and tools for the biological synthesis of mogrosides - Google Patents

Methods and tools for the biological synthesis of mogrosides

Info

Publication number
IL276781B1
IL276781B1 IL276781A IL27678120A IL276781B1 IL 276781 B1 IL276781 B1 IL 276781B1 IL 276781 A IL276781 A IL 276781A IL 27678120 A IL27678120 A IL 27678120A IL 276781 B1 IL276781 B1 IL 276781B1
Authority
IL
Israel
Prior art keywords
biosynthesis
materials
methods
mogroside compounds
mogroside
Prior art date
Application number
IL276781A
Other languages
English (en)
Hebrew (he)
Other versions
IL276781A (en
Original Assignee
Evolva Sa
Danstar Ferment Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US14/504,109 external-priority patent/US10011859B2/en
Application filed by Evolva Sa, Danstar Ferment Ag filed Critical Evolva Sa
Publication of IL276781A publication Critical patent/IL276781A/en
Publication of IL276781B1 publication Critical patent/IL276781B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P33/00—Preparation of steroids
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A23L27/36—Terpene glycosides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0073—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with NADH or NADPH as one donor, and incorporation of one atom of oxygen 1.14.13
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/56—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical directly bound to a condensed ring system having three or more carbocyclic rings, e.g. daunomycin, adriamycin
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04J—MULTIPLEX COMMUNICATION
    • H04J14/00—Optical multiplex systems
    • H04J14/02—Wavelength-division multiplex systems
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Computer Networks & Wireless Communication (AREA)
  • Signal Processing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
IL276781A 2014-10-01 2015-09-30 Methods and tools for the biological synthesis of mogrosides IL276781B1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US14/504,109 US10011859B2 (en) 2012-12-04 2014-10-01 Methods and materials for biosynthesis of mogroside compounds
US201462059136P 2014-10-02 2014-10-02
US201462087726P 2014-12-04 2014-12-04
US201462090836P 2014-12-11 2014-12-11
US201462091895P 2014-12-15 2014-12-15
US201562199115P 2015-07-30 2015-07-30
PCT/EP2015/072645 WO2016050890A2 (en) 2014-10-01 2015-09-30 Methods and materials for biosynthesis of mogroside compounds

Publications (2)

Publication Number Publication Date
IL276781A IL276781A (en) 2020-10-29
IL276781B1 true IL276781B1 (en) 2026-06-01

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
IL276781A IL276781B1 (en) 2014-10-01 2015-09-30 Methods and tools for the biological synthesis of mogrosides
IL251380A IL251380B (en) 2014-10-01 2017-03-26 Methods and materials for biosynthesis of mogroside compounds

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL251380A IL251380B (en) 2014-10-01 2017-03-26 Methods and materials for biosynthesis of mogroside compounds

Country Status (9)

Country Link
US (2) US10633685B2 (enExample)
EP (1) EP3201315A2 (enExample)
JP (1) JP2017529860A (enExample)
CN (1) CN107466320B (enExample)
AU (2) AU2015326892B2 (enExample)
CA (2) CA2963300C (enExample)
IL (2) IL276781B1 (enExample)
SG (2) SG10201902813XA (enExample)
WO (1) WO2016050890A2 (enExample)

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IL287789B2 (en) 2014-09-11 2024-05-01 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Mogroside compounds, methods for their preparation, preparations including them and their uses
CN105801658B (zh) * 2014-12-30 2019-03-19 北京农学院 甜甙及其类似物的制备及作为stat3、erk信号通路靶位点药物在抗肿瘤中的应用
JP6949838B2 (ja) 2015-10-29 2021-10-13 フィルメニッヒ インコーポレイテッドＦｉｒｍｅｎｉｃｈ Ｉｎｃｏｒｐｏｒａｔｅｄ 高甘味度甘味料
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
CN111108213B (zh) * 2017-05-03 2024-12-13 弗门尼舍公司 制备高强度甜味剂的方法
EP3638804A1 (en) 2017-06-15 2020-04-22 Evolva SA Production of mogroside compounds in recombinant hosts
CN109402163A (zh) * 2018-09-26 2019-03-01 怀化兴科创生物技术有限公司 新的罗汉果角鲨烯环氧酶基因（Sgsqe）的应用
CN109439676A (zh) * 2018-09-26 2019-03-08 怀化兴科创生物技术有限公司 罗汉果环氧化物水解酶Sgeph5基因的应用
CN113286523A (zh) * 2018-11-07 2021-08-20 弗门尼舍公司 制备高强度甜味剂的方法
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CN110066785B (zh) * 2019-01-30 2021-01-26 中国医学科学院药用植物研究所 一种罗汉果葫芦二烯醇合酶突变体及其构建方法和应用
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CN120230785A (zh) * 2020-03-17 2025-07-01 可口可乐公司 新的罗汉果苷生产系统和方法
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EP4314272A1 (en) * 2021-04-02 2024-02-07 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
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CN114807065B (zh) * 2021-06-07 2023-06-09 山西农业大学 一种酶的突变体
EP4314316A2 (en) 2021-06-29 2024-02-07 Firmenich Incorporated Methods for making high intensity sweeteners
CN114854722B (zh) * 2022-05-11 2024-01-09 成都普睿法药物研发有限公司 一种季也蒙念珠菌外切β-1,3-葡聚糖酶及其突变体及应用
NL2032683B1 (en) * 2022-07-18 2024-01-26 Sestina Bio Llc Bioproduction of isoprenoids
WO2024064695A2 (en) * 2022-09-19 2024-03-28 Elo Life Systems Mogroside compositions and methods of producing same
CN115896136B (zh) * 2022-12-22 2025-12-16 云南农业大学 与葫芦二烯醇羟化相关的细胞色素p450基因及其编码产物
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CN117417949B (zh) * 2023-10-19 2024-12-20 云南农业大学 雪胆细胞色素氧化酶HcCYP81Q58转基因酵母工程菌及其应用
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CN107466320A (zh) 2017-12-12
AU2022204012A1 (en) 2022-06-30
IL276781A (en) 2020-10-29
WO2016050890A2 (en) 2016-04-07
JP2017529860A (ja) 2017-10-12
IL251380B (en) 2020-09-30
CA2963300A1 (en) 2016-04-07
IL251380A0 (en) 2017-05-29
AU2015326892A2 (en) 2017-05-25
US11091787B2 (en) 2021-08-17
US20170247735A1 (en) 2017-08-31
AU2022204012C1 (en) 2024-09-05
US10633685B2 (en) 2020-04-28
US20200325517A1 (en) 2020-10-15
CA2963300C (en) 2024-04-30
SG10201902813XA (en) 2019-04-29
WO2016050890A3 (en) 2016-06-30
EP3201315A2 (en) 2017-08-09
CA3232630A1 (en) 2016-04-07
AU2015326892A1 (en) 2017-03-30
AU2015326892B2 (en) 2022-03-10
SG11201702123SA (en) 2017-04-27
CN107466320B (zh) 2021-11-05
AU2022204012B2 (en) 2024-06-27