IL258851B2 - התקן מכשיר מיקרופלואידי להרטבה חשמלית עם משטח הידרופופי בקישור קוולנטי - Google Patents
התקן מכשיר מיקרופלואידי להרטבה חשמלית עם משטח הידרופופי בקישור קוולנטיInfo
- Publication number
- IL258851B2 IL258851B2 IL258851A IL25885118A IL258851B2 IL 258851 B2 IL258851 B2 IL 258851B2 IL 258851 A IL258851 A IL 258851A IL 25885118 A IL25885118 A IL 25885118A IL 258851 B2 IL258851 B2 IL 258851B2
- Authority
- IL
- Israel
- Prior art keywords
- droplet
- microfluidic device
- microfluidic
- layer
- substrate
- Prior art date
Links
- 230000005661 hydrophobic surface Effects 0.000 title description 7
- 239000010410 layer Substances 0.000 claims description 319
- 239000000758 substrate Substances 0.000 claims description 286
- 239000002609 medium Substances 0.000 claims description 247
- 238000000034 method Methods 0.000 claims description 140
- 238000004720 dielectrophoresis Methods 0.000 claims description 128
- 230000002209 hydrophobic effect Effects 0.000 claims description 110
- 239000000463 material Substances 0.000 claims description 104
- 230000008569 process Effects 0.000 claims description 81
- 239000003989 dielectric material Substances 0.000 claims description 75
- 239000007788 liquid Substances 0.000 claims description 70
- 229910052739 hydrogen Inorganic materials 0.000 claims description 67
- 239000001257 hydrogen Substances 0.000 claims description 67
- 125000004435 hydrogen atom Chemical group [H]* 0.000 claims description 62
- 229910052731 fluorine Chemical group 0.000 claims description 59
- 239000011737 fluorine Chemical group 0.000 claims description 59
- 239000003153 chemical reaction reagent Substances 0.000 claims description 56
- 239000002356 single layer Substances 0.000 claims description 54
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical group [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 claims description 53
- 229910052710 silicon Inorganic materials 0.000 claims description 52
- 239000003446 ligand Substances 0.000 claims description 37
- 239000012736 aqueous medium Substances 0.000 claims description 27
- 229910021417 amorphous silicon Inorganic materials 0.000 claims description 24
- 239000011324 bead Substances 0.000 claims description 24
- 125000001153 fluoro group Chemical group F* 0.000 claims description 23
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 22
- -1 acyclic organic Chemical class 0.000 claims description 21
- 150000003961 organosilicon compounds Chemical class 0.000 claims description 21
- 238000006243 chemical reaction Methods 0.000 claims description 20
- 125000005647 linker group Chemical group 0.000 claims description 20
- 229910044991 metal oxide Inorganic materials 0.000 claims description 17
- 150000004706 metal oxides Chemical class 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 17
- 108020004414 DNA Proteins 0.000 claims description 12
- 229910052814 silicon oxide Inorganic materials 0.000 claims description 11
- 125000004437 phosphorous atom Chemical group 0.000 claims description 10
- 229910052698 phosphorus Inorganic materials 0.000 claims description 10
- 229910052581 Si3N4 Inorganic materials 0.000 claims description 9
- HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 claims description 9
- 230000003213 activating effect Effects 0.000 claims description 8
- 150000004767 nitrides Chemical class 0.000 claims description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 7
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical group O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims description 7
- 239000000377 silicon dioxide Substances 0.000 claims description 6
- 235000012239 silicon dioxide Nutrition 0.000 claims description 6
- 108020005196 Mitochondrial DNA Proteins 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 108020004999 messenger RNA Proteins 0.000 claims description 5
- 108091070501 miRNA Proteins 0.000 claims description 5
- 239000002679 microRNA Substances 0.000 claims description 5
- 238000003556 assay Methods 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 239000007850 fluorescent dye Substances 0.000 claims description 4
- 239000012139 lysis buffer Substances 0.000 claims description 4
- 238000013412 genome amplification Methods 0.000 claims description 3
- 239000002736 nonionic surfactant Substances 0.000 claims description 3
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims description 2
- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims description 2
- 238000010839 reverse transcription Methods 0.000 claims description 2
- PXGOKWXKJXAPGV-UHFFFAOYSA-N Fluorine Chemical group FF PXGOKWXKJXAPGV-UHFFFAOYSA-N 0.000 claims 4
- 230000009919 sequestration Effects 0.000 description 160
- 150000001875 compounds Chemical class 0.000 description 72
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 67
- 238000002955 isolation Methods 0.000 description 52
- 210000004027 cell Anatomy 0.000 description 46
- 230000004913 activation Effects 0.000 description 43
- 239000012530 fluid Substances 0.000 description 41
- 239000010703 silicon Substances 0.000 description 40
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical group [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 39
- 125000000217 alkyl group Chemical group 0.000 description 35
- 238000003384 imaging method Methods 0.000 description 29
- 150000002894 organic compounds Chemical class 0.000 description 25
- 230000033001 locomotion Effects 0.000 description 20
- 229920000642 polymer Polymers 0.000 description 20
- 239000000523 sample Substances 0.000 description 18
- 125000004432 carbon atom Chemical group C* 0.000 description 17
- 238000000231 atomic layer deposition Methods 0.000 description 16
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 15
- 229910052760 oxygen Inorganic materials 0.000 description 15
- 239000001301 oxygen Substances 0.000 description 15
- 230000035515 penetration Effects 0.000 description 15
- 238000009792 diffusion process Methods 0.000 description 13
- 239000004205 dimethyl polysiloxane Substances 0.000 description 13
- 230000007274 generation of a signal involved in cell-cell signaling Effects 0.000 description 13
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 13
- 239000004094 surface-active agent Substances 0.000 description 13
- 239000004020 conductor Substances 0.000 description 12
- 239000011521 glass Substances 0.000 description 12
- 229920001296 polysiloxane Polymers 0.000 description 12
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 11
- 239000012620 biological material Substances 0.000 description 11
- 125000003709 fluoroalkyl group Chemical group 0.000 description 11
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 10
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 10
- 239000004065 semiconductor Substances 0.000 description 10
- 125000001424 substituent group Chemical group 0.000 description 10
- 238000001816 cooling Methods 0.000 description 9
- 230000006870 function Effects 0.000 description 9
- 230000004048 modification Effects 0.000 description 9
- 238000012986 modification Methods 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 150000007523 nucleic acids Chemical class 0.000 description 9
- 239000003921 oil Substances 0.000 description 9
- 230000003287 optical effect Effects 0.000 description 9
- 238000000623 plasma-assisted chemical vapour deposition Methods 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 230000000269 nucleophilic effect Effects 0.000 description 8
- ABLZXFCXXLZCGV-UHFFFAOYSA-N phosphonic acid group Chemical group P(O)(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 102000004169 proteins and genes Human genes 0.000 description 8
- 230000028327 secretion Effects 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- 238000007740 vapor deposition Methods 0.000 description 8
- 229910052799 carbon Inorganic materials 0.000 description 7
- KPUWHANPEXNPJT-UHFFFAOYSA-N disiloxane Chemical class [SiH3]O[SiH3] KPUWHANPEXNPJT-UHFFFAOYSA-N 0.000 description 7
- 238000005530 etching Methods 0.000 description 7
- 239000007789 gas Substances 0.000 description 7
- 230000005484 gravity Effects 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 7
- 229910052751 metal Inorganic materials 0.000 description 7
- 239000002184 metal Substances 0.000 description 7
- 229920002545 silicone oil Polymers 0.000 description 7
- 239000002904 solvent Substances 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 125000001931 aliphatic group Chemical group 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 125000004429 atom Chemical group 0.000 description 6
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 6
- 229920001971 elastomer Polymers 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 238000012544 monitoring process Methods 0.000 description 6
- 125000005010 perfluoroalkyl group Chemical group 0.000 description 6
- 230000010412 perfusion Effects 0.000 description 6
- 239000002953 phosphate buffered saline Substances 0.000 description 6
- 238000012545 processing Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 125000000547 substituted alkyl group Chemical group 0.000 description 6
- 238000009736 wetting Methods 0.000 description 6
- 101000986989 Naja kaouthia Acidic phospholipase A2 CM-II Proteins 0.000 description 5
- 239000000853 adhesive Substances 0.000 description 5
- 230000001070 adhesive effect Effects 0.000 description 5
- 125000003118 aryl group Chemical group 0.000 description 5
- 238000004140 cleaning Methods 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 238000000151 deposition Methods 0.000 description 5
- 229910000449 hafnium oxide Inorganic materials 0.000 description 5
- WIHZLLGSGQNAGK-UHFFFAOYSA-N hafnium(4+);oxygen(2-) Chemical compound [O-2].[O-2].[Hf+4] WIHZLLGSGQNAGK-UHFFFAOYSA-N 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 5
- 125000004430 oxygen atom Chemical group O* 0.000 description 5
- 125000005373 siloxane group Chemical group [SiH2](O*)* 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000004593 Epoxy Substances 0.000 description 4
- 150000001336 alkenes Chemical group 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 239000006143 cell culture medium Substances 0.000 description 4
- 239000013592 cell lysate Substances 0.000 description 4
- 230000003750 conditioning effect Effects 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- CJNBYAVZURUTKZ-UHFFFAOYSA-N hafnium(iv) oxide Chemical compound O=[Hf]=O CJNBYAVZURUTKZ-UHFFFAOYSA-N 0.000 description 4
- WMCMKBBLRYJDNO-UHFFFAOYSA-N indium(3+) oxygen(2-) tantalum(5+) Chemical compound [O--].[O--].[O--].[O--].[In+3].[Ta+5] WMCMKBBLRYJDNO-UHFFFAOYSA-N 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 230000005693 optoelectronics Effects 0.000 description 4
- RVTZCBVAJQQJTK-UHFFFAOYSA-N oxygen(2-);zirconium(4+) Chemical compound [O-2].[O-2].[Zr+4] RVTZCBVAJQQJTK-UHFFFAOYSA-N 0.000 description 4
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
- 230000002829 reductive effect Effects 0.000 description 4
- 230000008961 swelling Effects 0.000 description 4
- 230000032258 transport Effects 0.000 description 4
- 229910001928 zirconium oxide Inorganic materials 0.000 description 4
- VCLJODPNBNEBKW-UHFFFAOYSA-N 2,2,4,4,6,8,8-heptamethylnonane Chemical compound CC(C)(C)CC(C)CC(C)(C)CC(C)(C)C VCLJODPNBNEBKW-UHFFFAOYSA-N 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 244000157072 Hylocereus undatus Species 0.000 description 3
- 235000018481 Hylocereus undatus Nutrition 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 229910052782 aluminium Inorganic materials 0.000 description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 150000001721 carbon Chemical group 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 238000004891 communication Methods 0.000 description 3
- 229920001577 copolymer Polymers 0.000 description 3
- 230000008021 deposition Effects 0.000 description 3
- 239000003599 detergent Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000000806 elastomer Substances 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 125000001072 heteroaryl group Chemical group 0.000 description 3
- 229930195733 hydrocarbon Natural products 0.000 description 3
- 150000002430 hydrocarbons Chemical class 0.000 description 3
- 230000001976 improved effect Effects 0.000 description 3
- AMGQUBHHOARCQH-UHFFFAOYSA-N indium;oxotin Chemical compound [In].[Sn]=O AMGQUBHHOARCQH-UHFFFAOYSA-N 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 239000002502 liposome Substances 0.000 description 3
- 238000011068 loading method Methods 0.000 description 3
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 3
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 3
- 239000011325 microbead Substances 0.000 description 3
- 239000011859 microparticle Substances 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 229920000620 organic polymer Polymers 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 238000009832 plasma treatment Methods 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 238000005381 potential energy Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000005060 rubber Substances 0.000 description 3
- 125000004469 siloxy group Chemical group [SiH3]O* 0.000 description 3
- 238000000527 sonication Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- HHBBIOLEJRWIGU-UHFFFAOYSA-N 4-ethoxy-1,1,1,2,2,3,3,4,5,6,6,6-dodecafluoro-5-(trifluoromethyl)hexane Chemical compound CCOC(F)(C(F)(C(F)(F)F)C(F)(F)F)C(F)(F)C(F)(F)C(F)(F)F HHBBIOLEJRWIGU-UHFFFAOYSA-N 0.000 description 2
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 108091005461 Nucleic proteins Proteins 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 125000002947 alkylene group Chemical group 0.000 description 2
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 2
- 125000003710 aryl alkyl group Chemical group 0.000 description 2
- 238000004422 calculation algorithm Methods 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 150000001768 cations Chemical class 0.000 description 2
- 239000008004 cell lysis buffer Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 239000011888 foil Substances 0.000 description 2
- 125000005843 halogen group Chemical group 0.000 description 2
- 125000004051 hexyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000009830 intercalation Methods 0.000 description 2
- 230000002687 intercalation Effects 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000004973 liquid crystal related substance Substances 0.000 description 2
- 238000001459 lithography Methods 0.000 description 2
- 230000002934 lysing effect Effects 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- QLOAVXSYZAJECW-UHFFFAOYSA-N methane;molecular fluorine Chemical group C.FF QLOAVXSYZAJECW-UHFFFAOYSA-N 0.000 description 2
- 125000000956 methoxy group Chemical group [H]C([H])([H])O* 0.000 description 2
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 2
- 125000004108 n-butyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- BPUBBGLMJRNUCC-UHFFFAOYSA-N oxygen(2-);tantalum(5+) Chemical compound [O-2].[O-2].[O-2].[O-2].[O-2].[Ta+5].[Ta+5] BPUBBGLMJRNUCC-UHFFFAOYSA-N 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000000059 patterning Methods 0.000 description 2
- 229920000136 polysorbate Polymers 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000000284 resting effect Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 229920005573 silicon-containing polymer Polymers 0.000 description 2
- 150000003384 small molecules Chemical class 0.000 description 2
- 238000013020 steam cleaning Methods 0.000 description 2
- 239000002344 surface layer Substances 0.000 description 2
- PBCFLUZVCVVTBY-UHFFFAOYSA-N tantalum pentoxide Inorganic materials O=[Ta](=O)O[Ta](=O)=O PBCFLUZVCVVTBY-UHFFFAOYSA-N 0.000 description 2
- 230000002123 temporal effect Effects 0.000 description 2
- 150000003573 thiols Chemical class 0.000 description 2
- 125000002023 trifluoromethyl group Chemical group FC(F)(F)* 0.000 description 2
- 239000012808 vapor phase Substances 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 125000006273 (C1-C3) alkyl group Chemical group 0.000 description 1
- 125000004169 (C1-C6) alkyl group Chemical group 0.000 description 1
- LHLRHWJTTUCDQA-UHFFFAOYSA-N 1-fluorodecane Chemical compound CCCCCCCCCCF LHLRHWJTTUCDQA-UHFFFAOYSA-N 0.000 description 1
- YHYBNVZCQIDLSQ-UHFFFAOYSA-N 1-fluorododecane Chemical compound CCCCCCCCCCCCF YHYBNVZCQIDLSQ-UHFFFAOYSA-N 0.000 description 1
- DHIVLKMGKIZOHF-UHFFFAOYSA-N 1-fluorooctane Chemical compound CCCCCCCCF DHIVLKMGKIZOHF-UHFFFAOYSA-N 0.000 description 1
- YRDSYPITPPQNED-UHFFFAOYSA-N 1-fluorotetradecane Chemical compound CCCCCCCCCCCCCCF YRDSYPITPPQNED-UHFFFAOYSA-N 0.000 description 1
- 239000004215 Carbon black (E152) Substances 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 241000252506 Characiformes Species 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 238000004566 IR spectroscopy Methods 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 238000004833 X-ray photoelectron spectroscopy Methods 0.000 description 1
- IUHFWCGCSVTMPG-UHFFFAOYSA-N [C].[C] Chemical class [C].[C] IUHFWCGCSVTMPG-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000002015 acyclic group Chemical group 0.000 description 1
- 150000001335 aliphatic alkanes Chemical group 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- PXTQQOLKZBLYDY-UHFFFAOYSA-N bis(2-ethylhexyl) carbonate Chemical compound CCCCC(CC)COC(=O)OCC(CC)CCCC PXTQQOLKZBLYDY-UHFFFAOYSA-N 0.000 description 1
- 239000007767 bonding agent Substances 0.000 description 1
- 125000001246 bromo group Chemical group Br* 0.000 description 1
- 125000000484 butyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000011203 carbon fibre reinforced carbon Substances 0.000 description 1
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000003833 cell viability Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- PBAYDYUZOSNJGU-UHFFFAOYSA-N chelidonic acid Natural products OC(=O)C1=CC(=O)C=C(C(O)=O)O1 PBAYDYUZOSNJGU-UHFFFAOYSA-N 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000013626 chemical specie Substances 0.000 description 1
- 125000001309 chloro group Chemical group Cl* 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 239000011370 conductive nanoparticle Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 125000004093 cyano group Chemical group *C#N 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 125000001891 dimethoxy group Chemical group [H]C([H])([H])O* 0.000 description 1
- 238000011143 downstream manufacturing Methods 0.000 description 1
- 238000000572 ellipsometry Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- SUHUKEQAOUOUJO-UHFFFAOYSA-N ethane-1,2-diol;2,4,7,9-tetramethyldec-5-yne-4,7-diol Chemical compound OCCO.CC(C)CC(C)(O)C#CC(C)(O)CC(C)C SUHUKEQAOUOUJO-UHFFFAOYSA-N 0.000 description 1
- 210000003527 eukaryotic cell Anatomy 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000005262 ferroelectric liquid crystals (FLCs) Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 229920005570 flexible polymer Polymers 0.000 description 1
- NBVXSUQYWXRMNV-UHFFFAOYSA-N fluoromethane Chemical compound FC NBVXSUQYWXRMNV-UHFFFAOYSA-N 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 125000004446 heteroarylalkyl group Chemical group 0.000 description 1
- 125000000592 heterocycloalkyl group Chemical group 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000006459 hydrosilylation reaction Methods 0.000 description 1
- 125000004356 hydroxy functional group Chemical group O* 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000011810 insulating material Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 125000000959 isobutyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])* 0.000 description 1
- 125000001972 isopentyl group Chemical group [H]C([H])([H])C([H])(C([H])([H])[H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 239000006193 liquid solution Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000007422 luminescence assay Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000000873 masking effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000007769 metal material Substances 0.000 description 1
- 238000001465 metallisation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000002048 multi walled nanotube Substances 0.000 description 1
- 125000000740 n-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 125000004123 n-propyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 239000002107 nanodisc Substances 0.000 description 1
- 239000002105 nanoparticle Substances 0.000 description 1
- 239000002070 nanowire Substances 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 125000001971 neopentyl group Chemical group [H]C([*])([H])C(C([H])([H])[H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- JRZJOMJEPLMPRA-UHFFFAOYSA-N olefin Natural products CCCCCCCC=C JRZJOMJEPLMPRA-UHFFFAOYSA-N 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 210000003463 organelle Anatomy 0.000 description 1
- 125000001147 pentyl group Chemical group C(CCCC)* 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920001983 poloxamer Polymers 0.000 description 1
- 229920000307 polymer substrate Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 210000001236 prokaryotic cell Anatomy 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 125000002572 propoxy group Chemical group [*]OC([H])([H])C(C([H])([H])[H])([H])[H] 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000002109 single walled nanotube Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 108020001572 subunits Proteins 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000005019 vapor deposition process Methods 0.000 description 1
- 238000001429 visible spectrum Methods 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 229910052724 xenon Inorganic materials 0.000 description 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
- B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
- B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/16—Surface properties and coatings
- B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
- B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0424—Dielectrophoretic forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
- B01L2400/0427—Electrowetting
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micromachines (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201562246605P | 2015-10-27 | 2015-10-27 | |
US201562247725P | 2015-10-28 | 2015-10-28 | |
US15/135,707 US10723988B2 (en) | 2015-04-22 | 2016-04-22 | Microfluidic cell culture |
US201662342131P | 2016-05-26 | 2016-05-26 | |
US201662410238P | 2016-10-19 | 2016-10-19 | |
PCT/US2016/059234 WO2017075295A1 (en) | 2015-10-27 | 2016-10-27 | Microfluidic electrowetting device apparatus having a covalently bound hydrophobic surface |
Publications (3)
Publication Number | Publication Date |
---|---|
IL258851A IL258851A (he) | 2018-06-28 |
IL258851B1 IL258851B1 (he) | 2023-04-01 |
IL258851B2 true IL258851B2 (he) | 2023-08-01 |
Family
ID=57281295
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
IL258851A IL258851B2 (he) | 2015-10-27 | 2018-04-22 | התקן מכשיר מיקרופלואידי להרטבה חשמלית עם משטח הידרופופי בקישור קוולנטי |
Country Status (10)
Country | Link |
---|---|
EP (2) | EP3862088A1 (he) |
JP (2) | JP6891169B2 (he) |
KR (1) | KR102426825B1 (he) |
CN (2) | CN114289087B (he) |
AU (2) | AU2016344171B2 (he) |
CA (1) | CA3001616C (he) |
IL (1) | IL258851B2 (he) |
SG (2) | SG11201802968VA (he) |
TW (2) | TWI781484B (he) |
WO (1) | WO2017075295A1 (he) |
Families Citing this family (41)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109154599A (zh) | 2016-03-24 | 2019-01-04 | 生物动力学公司 | 一次性射流卡盘及组件 |
CA3046827A1 (en) | 2016-12-12 | 2018-06-21 | xCella Biosciences, Inc. | Methods and systems for screening using microcapillary arrays |
WO2018200872A1 (en) * | 2017-04-26 | 2018-11-01 | Berkeley Lights, Inc. | Biological process systems and methods using microfluidic apparatus having an optimized electrowetting surface |
CA3067171A1 (en) * | 2017-06-21 | 2018-12-27 | Base4 Innovation Limited | Method for investigating molecules such as nucleic acids |
IL313689A (he) * | 2017-06-21 | 2024-08-01 | Lightcast Discovery Ltd | מכשיר מניפולציה למיקרו-טיפות |
CN107803228B (zh) * | 2017-11-06 | 2019-10-18 | 南京理工大学 | 一种自动分离水油混合液滴的装置及其分离方法 |
WO2019107231A1 (ja) * | 2017-11-29 | 2019-06-06 | 積水化学工業株式会社 | マイクロ流体チップ |
CN116121340A (zh) | 2017-12-19 | 2023-05-16 | 生物动力学公司 | 用于从生物样品中检测多种分析物的方法和装置 |
US20190262829A1 (en) * | 2018-02-28 | 2019-08-29 | Volta Labs, Inc. | Directing Motion of Droplets Using Differential Wetting |
US11883833B2 (en) | 2018-04-02 | 2024-01-30 | Biological Dynamics, Inc. | Dielectric materials |
KR102040286B1 (ko) * | 2018-07-09 | 2019-11-04 | 성균관대학교 산학협력단 | 종이기반 디지털 미세 유체역학기기의 제조 방법 |
EP3812451A4 (en) * | 2018-07-25 | 2021-08-11 | Able Corporation | CELL STIMULATION DEVICE, CELL STIMULATION PROCESS, PRODUCTION PROCESS FOR A CROP PRODUCT, PRODUCTION PROCESS FOR ISOLATED CELLS, AND CELL PROLIFERATION PROCESS |
WO2020024119A1 (en) * | 2018-08-01 | 2020-02-06 | University Of Macau | Apparatus and method for on-chip microfluids dispensing |
US10913067B2 (en) * | 2018-10-01 | 2021-02-09 | Sharp Life Science (Eu) Limited | Barrier droplet configurations against migration between droplets on AM-EWOD devices |
TWI763526B (zh) * | 2018-10-15 | 2022-05-01 | 美商電子墨水股份有限公司 | 用於將水性化學物質分配到表面的方法 |
KR102585756B1 (ko) * | 2018-11-12 | 2023-10-11 | 현대자동차주식회사 | 역전기습윤 에너지 수확장치 |
GB201909514D0 (en) * | 2018-11-20 | 2019-08-14 | Lightcast Discovery Ltd | Device and method for microdroplet detection of cells |
CN109487234A (zh) * | 2018-12-18 | 2019-03-19 | 湖北大学 | 超疏油涂层及其制备方法和应用 |
US20210220828A1 (en) * | 2019-01-15 | 2021-07-22 | Beijing Boe Optoelectronics Technology Co., Ltd. | Biological detection substrate, microfluidic chip and driving method thereof, microfluidic detection component |
CN111450906B (zh) * | 2019-01-22 | 2022-02-25 | 北京纳米能源与系统研究所 | 自驱动型电润湿阀门、纸基微流体芯片及免疫检测器件 |
CN109752856B (zh) * | 2019-03-08 | 2022-08-12 | 京东方科技集团股份有限公司 | 数字微流器件、制作方法、裸眼三维显示装置和显示方法 |
WO2020223077A1 (en) * | 2019-04-30 | 2020-11-05 | E Ink Corporation | Microfluidic devices and methods of making the same |
TWI745719B (zh) * | 2019-07-15 | 2021-11-11 | 長庚大學 | 奈米粒子控制量測系統及其運作方法 |
JP2023500623A (ja) * | 2019-10-25 | 2023-01-10 | バークレー ライツ,インコーポレイテッド | マイクロ流体デバイスを作動させるためのシステム |
CN111056525B (zh) * | 2019-11-12 | 2023-04-18 | 重庆大学 | 交流电浸润效应致微通道沸腾换热强化和流动不稳定性抑制方法 |
WO2021102134A1 (en) | 2019-11-20 | 2021-05-27 | E Ink Corporation | Spatially variable hydrophobic layers for digital microfluidics |
CN114945426A (zh) | 2020-01-17 | 2022-08-26 | 核酸有限公司 | 用于数字微流体的空间可变介电层 |
US11946901B2 (en) | 2020-01-27 | 2024-04-02 | Nuclera Ltd | Method for degassing liquid droplets by electrical actuation at higher temperatures |
JP2023513832A (ja) | 2020-02-18 | 2023-04-03 | ヌークレラ ヌクリークス, リミテッド | Ewodアレイの高周波ac駆動のためのアダプティブゲート駆動 |
JP2023514278A (ja) | 2020-02-19 | 2023-04-05 | ヌークレラ ヌクリークス, リミテッド | EWoDアレイの高周波数AC駆動のためのラッチ付きトランジスタ駆動 |
WO2021222061A1 (en) | 2020-04-27 | 2021-11-04 | Nuclera Nucleics Ltd. | Segmented top plate for variable driving and short protection for digital microfluidics |
CN112175824B (zh) * | 2020-09-17 | 2022-05-27 | 厦门德运芯准科技有限公司 | 一种基于数字微流控技术的全自动单细胞捕获芯片及其应用 |
CN114317250A (zh) * | 2020-09-30 | 2022-04-12 | 富佳生技股份有限公司 | 加热结构、检测芯片、核酸检测盒及核酸检测设备 |
JP2022058244A (ja) * | 2020-09-30 | 2022-04-11 | 富佳生技股▲ふん▼有限公司 | 核酸検出箱及び核酸検出デバイス |
US20220097052A1 (en) * | 2020-09-30 | 2022-03-31 | Icare Diagnostics International Co. Ltd. | Detection chip, nucleic acid detection kit, and nucleic acid detection device |
WO2022216283A1 (en) * | 2021-04-07 | 2022-10-13 | Hewlett-Packard Development Company, L.P. | Dielectric layer for microfluidic receptacle |
JP2023157546A (ja) * | 2022-04-15 | 2023-10-26 | Toppanホールディングス株式会社 | マイクロ流路チップおよびマイクロ流路チップの製造方法 |
TWI837762B (zh) * | 2022-08-10 | 2024-04-01 | 醫華生技股份有限公司 | 非接觸式分選裝置與其光感應結構、及生物微粒分選設備 |
WO2024081151A1 (en) * | 2022-10-11 | 2024-04-18 | Ohio State Innovation Foundation | Detection of an amphiphile using visual inspection of a ligand-modified substrate |
CN116060148B (zh) * | 2023-02-17 | 2024-06-07 | 西南石油大学 | 一种纳米通道原位可控疏水改性方法 |
CN117920372B (zh) * | 2024-03-18 | 2024-07-05 | 微纳动力(北京)科技有限责任公司 | 一种光电镊芯片及其制造方法 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20080225378A1 (en) * | 2007-03-13 | 2008-09-18 | Varioptic | Dielectric coatings for electrowetting applications |
US20090170186A1 (en) * | 2004-04-12 | 2009-07-02 | Ming Chiang Wu | Optoelectronic tweezers for microparticle and cell manipulation |
Family Cites Families (47)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6379929B1 (en) * | 1996-11-20 | 2002-04-30 | The Regents Of The University Of Michigan | Chip-based isothermal amplification devices and methods |
US6294063B1 (en) | 1999-02-12 | 2001-09-25 | Board Of Regents, The University Of Texas System | Method and apparatus for programmable fluidic processing |
CN1181337C (zh) * | 2000-08-08 | 2004-12-22 | 清华大学 | 微流体系统中实体分子的操纵方法及相关试剂盒 |
US6942776B2 (en) | 1999-05-18 | 2005-09-13 | Silicon Biosystems S.R.L. | Method and apparatus for the manipulation of particles by means of dielectrophoresis |
US6958132B2 (en) | 2002-05-31 | 2005-10-25 | The Regents Of The University Of California | Systems and methods for optical actuation of microfluidics based on opto-electrowetting |
JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
US6911132B2 (en) * | 2002-09-24 | 2005-06-28 | Duke University | Apparatus for manipulating droplets by electrowetting-based techniques |
US7041481B2 (en) | 2003-03-14 | 2006-05-09 | The Regents Of The University Of California | Chemical amplification based on fluid partitioning |
US20050164402A1 (en) * | 2003-07-14 | 2005-07-28 | Belisle Christopher M. | Sample presentation device |
WO2005047696A1 (en) * | 2003-11-17 | 2005-05-26 | Koninklijke Philips Electronics N.V. | System for manipulation of a body of fluid |
TWI258456B (en) * | 2005-05-31 | 2006-07-21 | Academia Sinica | Fluid driving device |
CA2636855C (en) | 2006-01-11 | 2016-09-27 | Raindance Technologies, Inc. | Microfluidic devices and methods of use in the formation and control of nanoreactors |
US20150107995A1 (en) * | 2006-04-18 | 2015-04-23 | Advanced Liquid Logic, Inc. | Droplet Actuator Devices and Methods for Manipulating Beads |
EP2021113A2 (en) | 2006-05-11 | 2009-02-11 | Raindance Technologies, Inc. | Microfluidic devices |
JP2008089752A (ja) * | 2006-09-29 | 2008-04-17 | Sony Corp | エレクトロウエッティング装置とこれを用いた可変焦点レンズ、光ピックアップ装置、光記録再生装置、液滴操作装置、光学素子、ズームレンズ、撮像装置、光変調装置及び表示装置 |
US20080153134A1 (en) * | 2006-12-22 | 2008-06-26 | Willy Wiyatno | Methods and apparatus for generating hydrophilic patterning of high density microplates using an amphiphilic polymer |
WO2008091848A2 (en) | 2007-01-22 | 2008-07-31 | Advanced Liquid Logic, Inc. | Surface assisted fluid loading and droplet dispensing |
WO2008119066A1 (en) | 2007-03-28 | 2008-10-02 | The Regents Of The University Of California | Single-sided lateral-field and phototransistor-based optoelectronic tweezers |
US8111465B2 (en) * | 2007-09-12 | 2012-02-07 | University Of Cincinnati | Electrofluidic devices, visual displays, and methods for making and operating such electrofluidic devices |
US9279435B2 (en) * | 2008-02-25 | 2016-03-08 | University of Washington through its Center for Communication | Vibration-driven droplet transport devices |
WO2009111723A1 (en) * | 2008-03-07 | 2009-09-11 | Drexel University | Electrowetting microarray printing system and methods for bioactive tissue construct manufacturing |
JP2010107908A (ja) * | 2008-10-31 | 2010-05-13 | Sony Corp | エレクトロウェッティング装置、可変焦点レンズ、光ピックアップ装置、光記録再生装置、液滴操作装置、光学素子、ズームレンズ、撮像装置、光変調装置、表示装置、ストロボ装置及びエレクトロウェッティング装置の駆動方法 |
CN101592627B (zh) * | 2009-03-19 | 2012-12-05 | 中国科学院苏州纳米技术与纳米仿生研究所 | 多通道高灵敏生物传感器的制作集成方法 |
FR2946658B1 (fr) * | 2009-06-11 | 2011-08-05 | Commissariat Energie Atomique | Dispositif microfluidique comportant deux couches hydrophobes assemblees l'une a l'autre et procede d'assemblage |
US8815070B2 (en) * | 2010-03-09 | 2014-08-26 | Sparkle Power, Inc. | Microelectrode array architecture |
KR20120066100A (ko) * | 2010-12-14 | 2012-06-22 | 한국전자통신연구원 | 혈액 분석용 소자 및 이를 이용한 혈액 분석 방법 |
CN102671723B (zh) * | 2011-02-17 | 2015-03-11 | 王崇智 | 介质上电润湿微电极阵列结构上的液滴处理方法 |
US9227200B2 (en) | 2011-06-03 | 2016-01-05 | The Regents Of The University Of California | Microfluidic devices with flexible optically transparent electrodes |
US8883014B2 (en) * | 2011-06-03 | 2014-11-11 | The Regents Of The University Of California | Monolithically formed EWOD device and method of making the same |
US20130062205A1 (en) * | 2011-09-14 | 2013-03-14 | Sharp Kabushiki Kaisha | Active matrix device for fluid control by electro-wetting and dielectrophoresis and method of driving |
CN102500436A (zh) * | 2011-09-28 | 2012-06-20 | 复旦大学 | 基于电润湿的单面二维驱动数字微流控芯片 |
US8821705B2 (en) * | 2011-11-25 | 2014-09-02 | Tecan Trading Ag | Digital microfluidics system with disposable cartridges |
AU2013207938B2 (en) * | 2012-01-10 | 2016-11-17 | President And Fellows Of Harvard College | Modification of surfaces for fluid and solid repellency |
US20140378339A1 (en) * | 2012-01-24 | 2014-12-25 | Katholieke Universiteit Euven | Patterning device |
CN102866193B (zh) * | 2012-09-04 | 2015-04-01 | 吴传勇 | 基于介电泳来操控液体中的粒子的器件及方法 |
US9857333B2 (en) * | 2012-10-31 | 2018-01-02 | Berkeley Lights, Inc. | Pens for biological micro-objects |
US9403172B2 (en) | 2012-11-08 | 2016-08-02 | Berkeley Lights, Inc. | Circuit based optoelectronic tweezers |
US9366647B2 (en) * | 2013-03-14 | 2016-06-14 | Taiwan Semiconductor Manufacturing Company, Ltd. | Optical detection for bio-entities |
US9239328B2 (en) * | 2012-12-17 | 2016-01-19 | Taiwan Semiconductor Manufacturing Company, Ltd. | Systems and methods for an integrated bio-entity manipulation and processing semiconductor device |
EP2869922B1 (en) * | 2013-01-09 | 2019-11-20 | Tecan Trading AG | Disposable cartridge for microfluidics systems |
US9453838B2 (en) * | 2013-03-12 | 2016-09-27 | Asociación Centro de Investigación Cooperative en Biomateriales | Methods for making microarrays and their uses |
WO2014167858A1 (ja) * | 2013-04-12 | 2014-10-16 | パナソニック株式会社 | 溶媒制御方法およびエレクトロウェッティング用溶媒 |
CN103470852A (zh) * | 2013-09-12 | 2013-12-25 | 东南大学 | 一种基于电润湿技术的微流体阀装置 |
EP3084492B1 (en) * | 2013-12-20 | 2022-08-17 | Universiteit Gent | Adiabatic coupler |
AU2015249294B2 (en) * | 2014-04-25 | 2020-02-27 | Berkeley Lights, Inc. | Providing DEP manipulation devices and controllable electrowetting devices in the same microfluidic apparatus |
US20150377831A1 (en) * | 2014-06-27 | 2015-12-31 | The Governing Council Of The University Of Toronto | Digital microfluidic devices and methods employing integrated nanostructured electrodeposited electrodes |
US9815056B2 (en) | 2014-12-05 | 2017-11-14 | The Regents Of The University Of California | Single sided light-actuated microfluidic device with integrated mesh ground |
-
2016
- 2016-10-27 CN CN202210023074.1A patent/CN114289087B/zh active Active
- 2016-10-27 JP JP2018519385A patent/JP6891169B2/ja active Active
- 2016-10-27 SG SG11201802968VA patent/SG11201802968VA/en unknown
- 2016-10-27 CN CN201680077445.7A patent/CN108472649B/zh active Active
- 2016-10-27 TW TW109142086A patent/TWI781484B/zh active
- 2016-10-27 WO PCT/US2016/059234 patent/WO2017075295A1/en active Application Filing
- 2016-10-27 SG SG10202107069UA patent/SG10202107069UA/en unknown
- 2016-10-27 KR KR1020187014919A patent/KR102426825B1/ko active IP Right Grant
- 2016-10-27 AU AU2016344171A patent/AU2016344171B2/en active Active
- 2016-10-27 EP EP20211621.6A patent/EP3862088A1/en active Pending
- 2016-10-27 CA CA3001616A patent/CA3001616C/en active Active
- 2016-10-27 EP EP16794477.6A patent/EP3370868B1/en active Active
- 2016-10-27 TW TW105134881A patent/TWI714656B/zh active
-
2018
- 2018-04-22 IL IL258851A patent/IL258851B2/he unknown
-
2021
- 2021-05-26 JP JP2021088097A patent/JP2021126656A/ja not_active Ceased
- 2021-12-17 AU AU2021286430A patent/AU2021286430A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20090170186A1 (en) * | 2004-04-12 | 2009-07-02 | Ming Chiang Wu | Optoelectronic tweezers for microparticle and cell manipulation |
US20080225378A1 (en) * | 2007-03-13 | 2008-09-18 | Varioptic | Dielectric coatings for electrowetting applications |
Non-Patent Citations (1)
Title |
---|
FURUTA, TSUTOMU, ET AL. , TSUTOMU, ET AL., FURUTA, 31 December 2012 (2012-12-31) * |
Also Published As
Publication number | Publication date |
---|---|
TWI781484B (zh) | 2022-10-21 |
JP6891169B2 (ja) | 2021-06-18 |
AU2016344171A1 (en) | 2018-05-10 |
KR102426825B1 (ko) | 2022-07-28 |
CA3001616C (en) | 2023-09-05 |
EP3862088A1 (en) | 2021-08-11 |
AU2021286430A1 (en) | 2022-01-20 |
WO2017075295A1 (en) | 2017-05-04 |
CA3001616A1 (en) | 2017-05-04 |
TW201726540A (zh) | 2017-08-01 |
AU2016344171B2 (en) | 2021-09-23 |
TWI714656B (zh) | 2021-01-01 |
JP2021126656A (ja) | 2021-09-02 |
WO2017075295A8 (en) | 2018-05-31 |
CN114289087B (zh) | 2024-03-26 |
SG10202107069UA (en) | 2021-07-29 |
CN108472649B (zh) | 2022-01-14 |
IL258851B1 (he) | 2023-04-01 |
KR20180072812A (ko) | 2018-06-29 |
CN114289087A (zh) | 2022-04-08 |
CN108472649A (zh) | 2018-08-31 |
EP3370868B1 (en) | 2020-12-09 |
IL258851A (he) | 2018-06-28 |
SG11201802968VA (en) | 2018-05-30 |
JP2018535088A (ja) | 2018-11-29 |
EP3370868A1 (en) | 2018-09-12 |
TW202126571A (zh) | 2021-07-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11964275B2 (en) | Microfluidic apparatus having an optimized electrowetting surface and related systems and methods | |
EP3370868B1 (en) | Microfluidic electrowetting device apparatus having a covalently bound hydrophobic surface | |
US10569271B2 (en) | Single-sided light-actuated microfluidic device with integrated mesh ground | |
US11192108B2 (en) | Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof | |
US20200171501A1 (en) | Biological Process Systems and Methods Using Microfluidic Apparatus Having an Optimized Electrowetting Surface | |
US10766033B2 (en) | Droplet generation in a microfluidic device having an optoelectrowetting configuration | |
US20160199837A1 (en) | Movement and selection of micro-objects in a microfluidic apparatus |