IL137649A - Method of adsorption chromatography - Google Patents
Method of adsorption chromatographyInfo
- Publication number
- IL137649A IL137649A IL13764998A IL13764998A IL137649A IL 137649 A IL137649 A IL 137649A IL 13764998 A IL13764998 A IL 13764998A IL 13764998 A IL13764998 A IL 13764998A IL 137649 A IL137649 A IL 137649A
- Authority
- IL
- Israel
- Prior art keywords
- liquid
- density
- approximately
- column
- sample molecule
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 100
- 238000005377 adsorption chromatography Methods 0.000 title description 6
- 239000007788 liquid Substances 0.000 claims abstract description 215
- 238000010828 elution Methods 0.000 claims abstract description 75
- 230000008569 process Effects 0.000 claims abstract description 46
- 239000003480 eluent Substances 0.000 claims abstract description 43
- 239000007787 solid Substances 0.000 claims abstract description 42
- 238000002156 mixing Methods 0.000 claims abstract description 30
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 27
- 238000004458 analytical method Methods 0.000 claims abstract description 5
- 238000011068 loading method Methods 0.000 claims abstract description 4
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 150
- 230000006872 improvement Effects 0.000 claims description 8
- 230000002829 reductive effect Effects 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 5
- 238000004440 column chromatography Methods 0.000 claims description 4
- 238000011140 membrane chromatography Methods 0.000 claims description 3
- 239000000243 solution Substances 0.000 description 62
- 239000000523 sample Substances 0.000 description 42
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 40
- 239000000872 buffer Substances 0.000 description 33
- 102000004169 proteins and genes Human genes 0.000 description 32
- 108090000623 proteins and genes Proteins 0.000 description 32
- 239000003795 chemical substances by application Substances 0.000 description 29
- 150000003839 salts Chemical class 0.000 description 29
- 239000007987 MES buffer Substances 0.000 description 27
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 22
- 239000011780 sodium chloride Substances 0.000 description 20
- 239000011534 wash buffer Substances 0.000 description 17
- 239000003463 adsorbent Substances 0.000 description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 9
- 239000000463 material Substances 0.000 description 9
- 239000000203 mixture Substances 0.000 description 7
- 238000001179 sorption measurement Methods 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 238000002835 absorbance Methods 0.000 description 6
- 230000003247 decreasing effect Effects 0.000 description 6
- 239000012149 elution buffer Substances 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 239000011347 resin Substances 0.000 description 5
- 229920005989 resin Polymers 0.000 description 5
- 230000002441 reversible effect Effects 0.000 description 5
- 239000011343 solid material Substances 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 4
- 238000001742 protein purification Methods 0.000 description 4
- 239000012460 protein solution Substances 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 238000011107 packed bed chromatography Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000011800 void material Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000005352 clarification Methods 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 238000001471 micro-filtration Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 102000004196 processed proteins & peptides Human genes 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 239000012488 sample solution Substances 0.000 description 2
- 238000013341 scale-up Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- -1 without limitation Substances 0.000 description 2
- 241000370685 Arge Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 230000031018 biological processes and functions Effects 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000005341 cation exchange Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000011097 chromatography purification Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013058 crude material Substances 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000012502 diagnostic product Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000011536 extraction buffer Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 238000004255 ion exchange chromatography Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011027 product recovery Methods 0.000 description 1
- 239000004627 regenerated cellulose Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
- 238000010977 unit operation Methods 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/20—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the sorbent material
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1807—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns using counter-currents, e.g. fluidised beds
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/265—Adsorption chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
- B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
- B01D15/1892—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns the sorbent material moving as a whole, e.g. continuous annular chromatography, true moving beds or centrifugal chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Health & Medical Sciences (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Peptides Or Proteins (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Saccharide Compounds (AREA)
- Separation Using Semi-Permeable Membranes (AREA)
- Liquid Developers In Electrophotography (AREA)
- Steroid Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US7520298P | 1998-02-18 | 1998-02-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IL137649A true IL137649A (en) | 2004-08-31 |
Family
ID=22124214
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL13764998A IL137649A (en) | 1998-02-18 | 1998-02-18 | Method of adsorption chromatography |
| IL13764999A IL137649A0 (en) | 1998-02-18 | 1999-02-16 | Adsorption chromatography |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IL13764999A IL137649A0 (en) | 1998-02-18 | 1999-02-16 | Adsorption chromatography |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6027650A (de) |
| EP (1) | EP1064066B1 (de) |
| JP (1) | JP2002503539A (de) |
| AT (1) | ATE250966T1 (de) |
| AU (1) | AU745806B2 (de) |
| CA (1) | CA2322048A1 (de) |
| DE (1) | DE69911754T2 (de) |
| DK (1) | DK1064066T3 (de) |
| IL (2) | IL137649A (de) |
| WO (1) | WO1999042193A1 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE245048T1 (de) * | 1998-10-31 | 2003-08-15 | Amersham Biosciences Ab | Chromatographisches verfahren mit verwendung eines wirbelbetts |
| US6290910B1 (en) | 1999-03-03 | 2001-09-18 | University Of North Florida | Continuously variable volume chamber for flow injection analysis |
| KR100409092B1 (ko) * | 2000-12-22 | 2003-12-11 | 주)녹십자 | 단백질의 제조 방법 |
| KR100372931B1 (ko) * | 2000-12-26 | 2003-02-25 | 변유량 | 팽창층 겔 흡착-이온교환 크로마토그래피법을 이용한 박테리오신의 분리 정제 방법 |
| US20070199899A1 (en) * | 2004-03-31 | 2007-08-30 | Andrew Alaska | Sweep-flow methods and clogging disrupters, for expanded bed chromatography of liquids with suspended particulates |
| EP1765866B1 (de) * | 2004-06-07 | 2014-01-08 | Therapure Biopharma Inc. | Isolierung von plasma- oder serumprotein |
| SE529259C2 (sv) * | 2005-08-31 | 2007-06-12 | Ge Healthcare Bio Sciences Ab | Tillverkning av kromarografimatriser, en kromarografimatris, en vätskekromatografikolonn, prcess för att isolera målföreningar och användning av en kromarografimatris för vätskekromatografi |
| WO2007058599A1 (en) * | 2005-11-21 | 2007-05-24 | Ge Healthcare Bio-Sciences Ab | Manufacture of a chromatography matrix |
| EP1972933A1 (de) * | 2007-03-23 | 2008-09-24 | Coöperatie AVEBE U.A. | Chromatographisches Säulesystem |
| CA2682333C (en) * | 2007-03-28 | 2015-05-05 | Upfront Chromatography A/S | Expanded bed column and disposable chromatography |
| AU2011343813B2 (en) * | 2010-12-15 | 2015-05-21 | Takeda Pharmaceutical Company Limited | Eluate collection using conductivity gradient |
| JP5621052B2 (ja) * | 2010-12-21 | 2014-11-05 | エフ．ホフマン−ラ ロシュアーゲーＦ．Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | クロマトグラフィー装置の特徴決定 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4411789A (en) * | 1981-08-21 | 1983-10-25 | Liburdy Robert P | Magnetic resonance chromatography |
| US4675113A (en) * | 1984-11-28 | 1987-06-23 | University Patents, Inc. | Affinity chromatography using dried calcium alginate-magnetite separation media in a magnetically stabilized fluidized bed |
| US5084184A (en) * | 1991-03-11 | 1992-01-28 | Exxon Chemical Patents Inc. | Positive focusing in a magnetically stabilized fluidized bed |
| SE9101149D0 (sv) * | 1991-04-17 | 1991-04-17 | Pharmacia Lkb Biotech | Beads for down stream processing |
| DE4203268A1 (de) * | 1992-02-05 | 1993-08-12 | Laevosan Gmbh & Co Kg | Verfahren zur chromatographischen trennung |
| JP3825798B2 (ja) * | 1994-01-18 | 2006-09-27 | ジェネンテク，インコーポレイテッド | ＩｇＥアンタゴニストを用いる寄生虫感染症の治療法 |
| US5702946A (en) * | 1994-03-03 | 1997-12-30 | Genentech, Inc. | Anti-IL-8 monoclonal antibodies for treatment of inflammatory disorders |
| CA2157219C (en) * | 1994-08-31 | 2010-10-05 | Munehiro Noda | Process for purifying recombinant human serum albumin |
| EP0746430A1 (de) * | 1995-01-04 | 1996-12-11 | Oberburg Engineering Ag | Drehturm mit expandierwerkzeugen |
| IL117645A (en) * | 1995-03-30 | 2005-08-31 | Genentech Inc | Vascular endothelial cell growth factor antagonists for use as medicaments in the treatment of age-related macular degeneration |
| US6096871A (en) * | 1995-04-14 | 2000-08-01 | Genentech, Inc. | Polypeptides altered to contain an epitope from the Fc region of an IgG molecule for increased half-life |
-
1998
- 1998-02-18 IL IL13764998A patent/IL137649A/en active IP Right Grant
-
1999
- 1999-02-16 EP EP99934253A patent/EP1064066B1/de not_active Revoked
- 1999-02-16 US US09/250,976 patent/US6027650A/en not_active Expired - Fee Related
- 1999-02-16 IL IL13764999A patent/IL137649A0/xx unknown
- 1999-02-16 DK DK99934253T patent/DK1064066T3/da active
- 1999-02-16 CA CA002322048A patent/CA2322048A1/en not_active Abandoned
- 1999-02-16 JP JP2000532198A patent/JP2002503539A/ja active Pending
- 1999-02-16 DE DE69911754T patent/DE69911754T2/de not_active Revoked
- 1999-02-16 WO PCT/US1999/003293 patent/WO1999042193A1/en not_active Ceased
- 1999-02-16 AU AU32948/99A patent/AU745806B2/en not_active Ceased
- 1999-02-16 AT AT99934253T patent/ATE250966T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2002503539A (ja) | 2002-02-05 |
| WO1999042193A1 (en) | 1999-08-26 |
| AU745806B2 (en) | 2002-04-11 |
| ATE250966T1 (de) | 2003-10-15 |
| US6027650A (en) | 2000-02-22 |
| CA2322048A1 (en) | 1999-08-26 |
| DE69911754D1 (en) | 2003-11-06 |
| DE69911754T2 (de) | 2004-07-29 |
| WO1999042193A9 (en) | 1999-11-11 |
| DK1064066T3 (da) | 2003-12-15 |
| EP1064066A1 (de) | 2001-01-03 |
| EP1064066B1 (de) | 2003-10-01 |
| AU3294899A (en) | 1999-09-06 |
| IL137649A0 (en) | 2001-10-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FF | Patent granted | ||
| KB | Patent renewed | ||
| KB | Patent renewed |