IE42566B1 - Process for the enrichment of pregnancy-specific -glycoprotein - Google Patents
Process for the enrichment of pregnancy-specific -glycoproteinInfo
- Publication number
- IE42566B1 IE42566B1 IE817/76A IE81776A IE42566B1 IE 42566 B1 IE42566 B1 IE 42566B1 IE 817/76 A IE817/76 A IE 817/76A IE 81776 A IE81776 A IE 81776A IE 42566 B1 IE42566 B1 IE 42566B1
- Authority
- IE
- Ireland
- Prior art keywords
- glycoprotein
- pregnancy
- specific
- carrier
- antibody
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 34
- 230000035935 pregnancy Effects 0.000 title claims abstract description 17
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 10
- 239000000126 substance Substances 0.000 claims abstract description 9
- 239000000284 extract Substances 0.000 claims abstract description 6
- 210000002966 serum Anatomy 0.000 claims abstract description 6
- 239000012736 aqueous medium Substances 0.000 claims abstract description 5
- 210000002700 urine Anatomy 0.000 claims abstract description 5
- 239000007791 liquid phase Substances 0.000 claims abstract description 4
- 239000000243 solution Substances 0.000 claims description 16
- 150000003839 salts Chemical class 0.000 claims description 13
- 239000003463 adsorbent Substances 0.000 claims description 10
- 102000003886 Glycoproteins Human genes 0.000 claims description 8
- 108090000288 Glycoproteins Proteins 0.000 claims description 8
- 238000002955 isolation Methods 0.000 claims description 5
- 150000001720 carbohydrates Chemical class 0.000 claims description 4
- 230000003169 placental effect Effects 0.000 claims description 4
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 claims description 4
- 239000000427 antigen Substances 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 230000003196 chaotropic effect Effects 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 2
- 229920000936 Agarose Polymers 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 239000004202 carbamide Substances 0.000 claims description 2
- 239000001913 cellulose Substances 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 claims description 2
- 229920001577 copolymer Polymers 0.000 claims description 2
- 238000000502 dialysis Methods 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 239000012460 protein solution Substances 0.000 claims description 2
- 229920003002 synthetic resin Polymers 0.000 claims description 2
- 239000000057 synthetic resin Substances 0.000 claims description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 1
- 239000005977 Ethylene Substances 0.000 claims 1
- FPYJFEHAWHCUMM-UHFFFAOYSA-N maleic anhydride Chemical compound O=C1OC(=O)C=C1 FPYJFEHAWHCUMM-UHFFFAOYSA-N 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims 1
- VGTPCRGMBIAPIM-UHFFFAOYSA-M sodium thiocyanate Chemical compound [Na+].[S-]C#N VGTPCRGMBIAPIM-UHFFFAOYSA-M 0.000 claims 1
- 210000002826 placenta Anatomy 0.000 abstract description 2
- 239000007858 starting material Substances 0.000 abstract 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000000872 buffer Substances 0.000 description 4
- 238000010494 dissociation reaction Methods 0.000 description 4
- 230000005593 dissociations Effects 0.000 description 4
- 229910052588 hydroxylapatite Inorganic materials 0.000 description 4
- 230000007935 neutral effect Effects 0.000 description 4
- XYJRXVWERLGGKC-UHFFFAOYSA-D pentacalcium;hydroxide;triphosphate Chemical compound [OH-].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O XYJRXVWERLGGKC-UHFFFAOYSA-D 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 238000011403 purification operation Methods 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- YYXLGGIKSIZHSF-UHFFFAOYSA-N ethene;furan-2,5-dione Chemical compound C=C.O=C1OC(=O)C=C1 YYXLGGIKSIZHSF-UHFFFAOYSA-N 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- -1 for example Polymers 0.000 description 1
- 239000007973 glycine-HCl buffer Substances 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N hydrochloric acid Substances Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- NQMRYBIKMRVZLB-UHFFFAOYSA-N methylamine hydrochloride Chemical compound [Cl-].[NH3+]C NQMRYBIKMRVZLB-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000011541 reaction mixture Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000012064 sodium phosphate buffer Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4715—Pregnancy proteins, e.g. placenta proteins, alpha-feto-protein, pregnancy specific beta glycoprotein
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Reproductive Health (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Endocrinology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Cell Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Food Science & Technology (AREA)
- Gynecology & Obstetrics (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US05/569,476 US4065445A (en) | 1971-09-29 | 1975-04-18 | Pregnancy-specific β1 -glycoprotein and process for isolating it |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE42566L IE42566L (en) | 1976-10-18 |
| IE42566B1 true IE42566B1 (en) | 1980-09-10 |
Family
ID=24275609
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE817/76A IE42566B1 (en) | 1975-04-18 | 1976-04-15 | Process for the enrichment of pregnancy-specific -glycoprotein |
| IE818/76A IE42679B1 (en) | 1975-04-18 | 1976-04-15 | Process for the purification of pregnancy-specific -glycoprotein |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE818/76A IE42679B1 (en) | 1975-04-18 | 1976-04-15 | Process for the purification of pregnancy-specific -glycoprotein |
Country Status (18)
| Country | Link |
|---|---|
| JP (2) | JPS51133406A ( ) |
| AT (2) | AT344920B ( ) |
| AU (2) | AU503288B2 ( ) |
| BE (2) | BE840913A ( ) |
| CA (2) | CA1065305A ( ) |
| DE (1) | DE2612479A1 ( ) |
| DK (2) | DK173576A ( ) |
| ES (2) | ES446944A1 ( ) |
| FI (2) | FI56184C ( ) |
| FR (2) | FR2307815A1 ( ) |
| GB (2) | GB1541454A ( ) |
| IE (2) | IE42566B1 ( ) |
| IT (1) | IT1060215B ( ) |
| LU (2) | LU74783A1 ( ) |
| NL (2) | NL7603902A ( ) |
| NO (2) | NO761306L ( ) |
| NZ (1) | NZ180619A ( ) |
| SE (2) | SE419340B ( ) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2720704C2 (de) * | 1977-05-07 | 1986-09-25 | Behringwerke Ag, 3550 Marburg | Neues Glycoprotein, Verfahren zu seiner Herstellung und seine Verwendung |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5325030B2 ( ) * | 1972-12-19 | 1978-07-24 |
-
1976
- 1976-03-24 DE DE19762612479 patent/DE2612479A1/de active Pending
- 1976-04-12 ES ES446944A patent/ES446944A1/es not_active Expired
- 1976-04-12 ES ES446939A patent/ES446939A1/es not_active Expired
- 1976-04-13 NL NL7603902A patent/NL7603902A/xx not_active Application Discontinuation
- 1976-04-13 NL NL7603903A patent/NL7603903A/xx not_active Application Discontinuation
- 1976-04-14 DK DK173576A patent/DK173576A/da active IP Right Grant
- 1976-04-14 NO NO761306A patent/NO761306L/no unknown
- 1976-04-14 FI FI761017A patent/FI56184C/fi not_active IP Right Cessation
- 1976-04-14 NO NO761305A patent/NO145405C/no unknown
- 1976-04-14 DK DK173876A patent/DK173876A/da active IP Right Grant
- 1976-04-14 FI FI761016A patent/FI54486C/fi not_active IP Right Cessation
- 1976-04-15 SE SE7604484A patent/SE419340B/xx unknown
- 1976-04-15 GB GB15558/76A patent/GB1541454A/en not_active Expired
- 1976-04-15 AU AU13081/76A patent/AU503288B2/en not_active Expired
- 1976-04-15 GB GB15557/76A patent/GB1541453A/en not_active Expired
- 1976-04-15 CA CA250,426A patent/CA1065305A/en not_active Expired
- 1976-04-15 SE SE7604483A patent/SE7604483L/ not_active Application Discontinuation
- 1976-04-15 IE IE817/76A patent/IE42566B1/en unknown
- 1976-04-15 NZ NZ180619A patent/NZ180619A/xx unknown
- 1976-04-15 IE IE818/76A patent/IE42679B1/en unknown
- 1976-04-15 CA CA250,439A patent/CA1065307A/en not_active Expired
- 1976-04-15 AU AU13082/76A patent/AU503342B2/en not_active Expired
- 1976-04-16 AT AT284176A patent/AT344920B/de not_active IP Right Cessation
- 1976-04-16 AT AT284276A patent/AT344734B/de not_active IP Right Cessation
- 1976-04-16 IT IT22445/76A patent/IT1060215B/it active
- 1976-04-16 LU LU74783A patent/LU74783A1/xx unknown
- 1976-04-16 LU LU74782A patent/LU74782A1/xx unknown
- 1976-04-16 FR FR7611367A patent/FR2307815A1/fr active Granted
- 1976-04-16 FR FR7611372A patent/FR2307816A1/fr active Granted
- 1976-04-17 JP JP51044152A patent/JPS51133406A/ja active Granted
- 1976-04-17 JP JP51044151A patent/JPS51133408A/ja active Pending
- 1976-04-20 BE BE166291A patent/BE840913A/xx not_active IP Right Cessation
- 1976-04-20 BE BE166290A patent/BE840912A/xx unknown
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Baumstark et al. | A preparative method for the separation of 7S gamma globulin from human serum | |
| EP0253845B1 (en) | Tangential flow affinity ultrafiltration | |
| JPS5839576B2 (ja) | 生物学的巨大分子を可逆的に固定しうる新規物質並びにその製造方法 | |
| US4163714A (en) | Separating substances with pressure-driven affinity sorption membranes | |
| CA2315750A1 (en) | A process for preparing human proinsulin | |
| US4191533A (en) | Pregnancy-specific β1 -glycoprotein and process for isolating it | |
| GB2179947A (en) | Process for the extraction of proteins from milk | |
| US6001974A (en) | Method of separating albumin from serum by ion-exchange chromatography with membrane adsorbers | |
| CA1101847A (en) | Process for the concentration of a placenta-specific glycoprotein | |
| US3639559A (en) | Method of isolating antigens and/or antibodies from protein mixtur | |
| US4065445A (en) | Pregnancy-specific β1 -glycoprotein and process for isolating it | |
| US5055557A (en) | Ultrapurification of factor IX and other vitamin K-dependent proteins | |
| US5055558A (en) | Method for the selective deproteinization of whey | |
| CA1252744A (en) | Ultrapurification of factor ix and other vitamin k- dependent proteins | |
| Turková | Bioaffinity chromatography | |
| US3959249A (en) | Method for isolating transferrines from biological materials | |
| IE42566B1 (en) | Process for the enrichment of pregnancy-specific -glycoprotein | |
| Vannier et al. | The preparation and properties of a hapten-cellulose antibody adsorbent | |
| US4018885A (en) | Steroid-binding globulin and process for preparing it and antibodies thereto | |
| JPS62259596A (ja) | ハイブリツドタンパク質の精製方法 | |
| WO1991014706A2 (en) | Lectin affinity membranes and methods for using them | |
| JPH0679172A (ja) | クロマトグラフィー剤およびタンパク質、ポリペプチドまたは金属の分離のためのその使用法 | |
| US4859766A (en) | Purified appetite-regulating substances of biological orgin, their antibodies, immunocomplexes of the appetite--regulating substances formed with the antibodies and processes for preparing same | |
| KR100341297B1 (ko) | 재조합활성형프로인슐린의분리.정제방법 | |
| JPS6141548B2 ( ) |