HUP9801188A2 - Eljárás transzgenikus állatokból származó magasabbrendű transzkripciós komplexek tisztítására - Google Patents

Eljárás transzgenikus állatokból származó magasabbrendű transzkripciós komplexek tisztítására

Info

Publication number
HUP9801188A2
HUP9801188A2 HU9801188A HUP9801188A HUP9801188A2 HU P9801188 A2 HUP9801188 A2 HU P9801188A2 HU 9801188 A HU9801188 A HU 9801188A HU P9801188 A HUP9801188 A HU P9801188A HU P9801188 A2 HUP9801188 A2 HU P9801188A2
Authority
HU
Hungary
Prior art keywords
transgenic animals
purification
higher order
transcription complexes
tbp
Prior art date
Application number
HU9801188A
Other languages
English (en)
Inventor
Erick Berglund
Bernd Kirschbaum
Michael Meisterernst
Greg Polites
Original Assignee
Hoechst Ag.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst Ag. filed Critical Hoechst Ag.
Publication of HU9801188D0 publication Critical patent/HU9801188D0/hu
Publication of HUP9801188A2 publication Critical patent/HUP9801188A2/hu
Publication of HUP9801188A3 publication Critical patent/HUP9801188A3/hu

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

A találmány epitóppal tőldőtt TATA-dőbőz fehérjét (TBP) kódőló DNS-ttartalmazó transzgénekkel, az epitóppal tőldőtt TBP-t kifejezőtranszgenikűs állatők előállításával, valamint mindezek alkalmazásávalfőglalkőzik sőkféle eűkarióta szövetből és sejttípűsból való (új)transzkripciós faktőrők és transzkripciós kőmplexek affinitásőstisztításáhőz. ŕ
HU9801188A 1997-05-26 1998-05-25 Purification of higher order transcription complexes from transgenic animals HUP9801188A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97108433 1997-05-26

Publications (3)

Publication Number Publication Date
HU9801188D0 HU9801188D0 (en) 1998-09-28
HUP9801188A2 true HUP9801188A2 (hu) 1999-03-29
HUP9801188A3 HUP9801188A3 (en) 2005-05-30

Family

ID=8226829

Family Applications (1)

Application Number Title Priority Date Filing Date
HU9801188A HUP9801188A3 (en) 1997-05-26 1998-05-25 Purification of higher order transcription complexes from transgenic animals

Country Status (15)

Country Link
US (2) US20020157127A1 (hu)
JP (1) JPH114638A (hu)
KR (1) KR100607527B1 (hu)
CN (1) CN1200235A (hu)
AR (1) AR012743A1 (hu)
AT (1) ATE443132T1 (hu)
AU (1) AU748461B2 (hu)
BR (1) BR9801703A (hu)
CA (1) CA2232806A1 (hu)
CZ (1) CZ160198A3 (hu)
DE (1) DE69841149D1 (hu)
HU (1) HUP9801188A3 (hu)
ID (1) ID20397A (hu)
PL (1) PL197770B1 (hu)
TR (1) TR199800920A2 (hu)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4850047A (en) * 1986-08-29 1989-07-18 Fujitsu Limited Optical bus communication system utilizing frame format signals
WO2004095029A1 (ja) * 2003-04-23 2004-11-04 Olympus Corporation 一分子蛍光分析により核酸と核酸結合タンパク質との結合を検出する方法
WO2007086382A1 (ja) * 2006-01-24 2007-08-02 Nagoya City University ヒト関節リウマチの病態を再現するトランスジェニック非ヒト哺乳動物
CN101864420B (zh) * 2009-05-06 2012-05-23 中国人民解放军军事医学科学院生物工程研究所 一种用于hek293细胞高效表达外源基因的表达载体

Also Published As

Publication number Publication date
HU9801188D0 (en) 1998-09-28
CN1200235A (zh) 1998-12-02
AU6806898A (en) 1998-11-26
BR9801703A (pt) 2000-01-11
US20020157127A1 (en) 2002-10-24
AU748461B2 (en) 2002-06-06
TR199800920A2 (xx) 1998-12-21
AR012743A1 (es) 2000-11-08
KR100607527B1 (ko) 2006-09-22
PL326482A1 (en) 1998-12-07
DE69841149D1 (de) 2009-10-29
KR19980087337A (ko) 1998-12-05
ID20397A (id) 1998-12-03
CZ160198A3 (cs) 1998-12-16
CA2232806A1 (en) 1998-11-26
ATE443132T1 (de) 2009-10-15
JPH114638A (ja) 1999-01-12
PL197770B1 (pl) 2008-04-30
US20050268350A1 (en) 2005-12-01
HUP9801188A3 (en) 2005-05-30

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Legal Events

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FD9A Lapse of provisional protection due to non-payment of fees