HUP0200945A3 - Surrogate cell-based system and method for assaying the activity of hapatitis c virus ns3 protease - Google Patents

Surrogate cell-based system and method for assaying the activity of hapatitis c virus ns3 protease

Info

Publication number
HUP0200945A3
HUP0200945A3 HU0200945A HUP0200945A HUP0200945A3 HU P0200945 A3 HUP0200945 A3 HU P0200945A3 HU 0200945 A HU0200945 A HU 0200945A HU P0200945 A HUP0200945 A HU P0200945A HU P0200945 A3 HUP0200945 A3 HU P0200945A3
Authority
HU
Hungary
Prior art keywords
hapatitis
assaying
protease
virus
activity
Prior art date
Application number
HU0200945A
Other languages
English (en)
Original Assignee
Boehringer Ingelheim Ca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Ca Ltd filed Critical Boehringer Ingelheim Ca Ltd
Publication of HUP0200945A2 publication Critical patent/HUP0200945A2/hu
Publication of HUP0200945A3 publication Critical patent/HUP0200945A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
HU0200945A 1999-05-04 2000-05-01 Surrogate cell-based system and method for assaying the activity of hapatitis c virus ns3 protease HUP0200945A3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13236099P 1999-05-04 1999-05-04

Publications (2)

Publication Number Publication Date
HUP0200945A2 HUP0200945A2 (en) 2002-08-28
HUP0200945A3 true HUP0200945A3 (en) 2004-07-28

Family

ID=22453660

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0200945A HUP0200945A3 (en) 1999-05-04 2000-05-01 Surrogate cell-based system and method for assaying the activity of hapatitis c virus ns3 protease

Country Status (14)

Country Link
US (3) US7276373B2 (hu)
EP (1) EP1177211B1 (hu)
JP (1) JP4782927B2 (hu)
AT (1) ATE386746T1 (hu)
AU (1) AU772386B2 (hu)
CA (1) CA2365262C (hu)
DE (1) DE60038113T2 (hu)
DK (1) DK1177211T3 (hu)
ES (1) ES2300262T3 (hu)
HU (1) HUP0200945A3 (hu)
IL (2) IL145376A0 (hu)
MX (1) MXPA01011045A (hu)
NZ (1) NZ515861A (hu)
WO (1) WO2000066623A2 (hu)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2365262C (en) * 1999-05-04 2008-06-10 Boehringer Ingelheim (Canada) Ltd. Surrogate cell-based system and method for assaying the activity of hepatitis c virus ns3 protease
WO2001002601A2 (en) * 1999-07-07 2001-01-11 Du Pont Pharmaceuticals Company Cell-based assay systems for examining hcv ns3 protease activity
US6986892B1 (en) 1999-11-24 2006-01-17 Chiron Corporation Immunogenic Hepatitis C virus non-structural polypeptides
ATE376558T1 (de) * 1999-11-24 2007-11-15 Novartis Vaccines & Diagnostic Neues nichtstrukturelles hcv polypeptid
US6921634B2 (en) * 1999-12-23 2005-07-26 Board Of Regents, The University Of Texas System Replication competent hepatitus C virus and methods of use
CA2370803A1 (en) * 2000-02-25 2001-08-30 Cambridge Drug Discovery, Ltd. Methods for identifying a protease inhibitor
JP2004516039A (ja) 2000-12-22 2004-06-03 ベーリンガー インゲルハイム （カナダ） リミテッド Ｃ型肝炎ウイルスからの自己複製ｒｎａ分子
DE10234418A1 (de) * 2002-07-29 2004-02-12 Bayer Ag Verfahren zur Untersuchung und Beurteilung von Virusenzymen im HTS
AU2004272114B2 (en) * 2003-09-12 2010-11-04 Vertex Pharmaceuticals Incorporated Animal model for protease activity and liver damage
RS20110578A3 (en) 2003-10-14 2016-02-29 F. Hoffmann-La Roche Ltd MACROCYCLIC CARBOXYLIC ACIDS AND ACYLSULPHONAMIDES AS HCV REPLICATION INHIBITORS
CN102816170A (zh) 2005-07-25 2012-12-12 因特蒙公司 C型肝炎病毒复制的新颖大环抑制剂
AU2006301966A1 (en) 2005-10-11 2007-04-19 Array Biopharma, Inc. Compounds and methods for inhibiting hepatitis C viral replication
CA2629343A1 (en) 2005-11-11 2007-05-24 Vertex Pharmaceuticals, Inc. Hepatitis c virus variants
US7705138B2 (en) * 2005-11-11 2010-04-27 Vertex Pharmaceuticals Incorporated Hepatitis C virus variants
WO2013027217A1 (en) * 2011-08-22 2013-02-28 Ramot At Tel-Aviv University Ltd. Zymoxins and methods of using the same
AU2013341227A1 (en) * 2012-11-07 2015-05-07 Gilead Sciences, Inc. HCV genotype 6 replicons
WO2019023164A1 (en) * 2017-07-24 2019-01-31 The Board Of Trustees Of The Leland Stanford Junior University COMPOSITIONS AND METHODS FOR INDUCING PROTEIN FUNCTION
WO2022094463A1 (en) * 2020-11-02 2022-05-05 Regents Of The University Of Minnesota Live cell assay for protease inhibition

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3269642B2 (ja) * 1990-06-25 2002-03-25 財団法人阪大微生物病研究会 非Ａ非Ｂ型肝炎ウイルス遺伝子ｃＤＮＡおよび抗原ポリペプチド
FI913068A (fi) * 1990-06-25 1991-12-26 Univ Osaka Res Found Non-a-, non-b-hepatitisviruspartiklar.
US5861267A (en) * 1995-05-01 1999-01-19 Vertex Pharmaceuticals Incorporated Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
IT1277914B1 (it) * 1995-08-22 1997-11-12 Angeletti P Ist Richerche Bio Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e
KR100209095B1 (ko) * 1996-06-28 1999-07-15 성재갑 C형 간염 바이러스의 프로테아제의 활성을 측정할 수 있는 c형 간염 대체 바이러스, 그 재조합 유전자 및 그 용도
US6280940B1 (en) * 1998-08-05 2001-08-28 Agouron Pharmaceuticals, Inc. Reporter gene system for use in cell-based assessment of inhibitors of the Hepatitis C virus protease
US6117639A (en) * 1998-08-31 2000-09-12 Vertex Pharmaceuticals Incorporated Fusion proteins, DNA molecules, vectors, and host cells useful for measuring protease activity
CA2365262C (en) 1999-05-04 2008-06-10 Boehringer Ingelheim (Canada) Ltd. Surrogate cell-based system and method for assaying the activity of hepatitis c virus ns3 protease

Also Published As

Publication number Publication date
EP1177211B1 (en) 2008-02-20
HUP0200945A2 (en) 2002-08-28
NZ515861A (en) 2004-08-27
AU4282500A (en) 2000-11-17
US20030162169A1 (en) 2003-08-28
US7276373B2 (en) 2007-10-02
DK1177211T3 (da) 2008-06-02
WO2000066623A3 (en) 2001-03-22
IL145376A (en) 2009-09-01
IL145376A0 (en) 2002-06-30
JP2002542812A (ja) 2002-12-17
EP1177211A2 (en) 2002-02-06
JP4782927B2 (ja) 2011-09-28
CA2365262A1 (en) 2000-11-09
US7838207B2 (en) 2010-11-23
US20050227226A1 (en) 2005-10-13
DE60038113T2 (de) 2009-02-12
MXPA01011045A (es) 2002-07-22
DE60038113D1 (de) 2008-04-03
ATE386746T1 (de) 2008-03-15
US20030162168A1 (en) 2003-08-28
WO2000066623A2 (en) 2000-11-09
AU772386B2 (en) 2004-04-29
CA2365262C (en) 2008-06-10
ES2300262T3 (es) 2008-06-16

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Legal Events

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FD9A Lapse of provisional protection due to non-payment of fees