DK1177211T3 - Surrogatcellebaseret system og fremgangsmåde til at analysere aktiviteten for Hepatitis C-virus NS3-protease - Google Patents

Surrogatcellebaseret system og fremgangsmåde til at analysere aktiviteten for Hepatitis C-virus NS3-protease

Info

Publication number
DK1177211T3
DK1177211T3 DK00922396T DK00922396T DK1177211T3 DK 1177211 T3 DK1177211 T3 DK 1177211T3 DK 00922396 T DK00922396 T DK 00922396T DK 00922396 T DK00922396 T DK 00922396T DK 1177211 T3 DK1177211 T3 DK 1177211T3
Authority
DK
Denmark
Prior art keywords
protease
hcv
transactivator
polyprotein
fragment
Prior art date
Application number
DK00922396T
Other languages
English (en)
Inventor
Charles Pellerin
Daniel Lamarre
Original Assignee
Boehringer Ingelheim Ca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Ca Ltd filed Critical Boehringer Ingelheim Ca Ltd
Application granted granted Critical
Publication of DK1177211T3 publication Critical patent/DK1177211T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/24011—Poxviridae
    • C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
    • C12N2710/24141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DK00922396T 1999-05-04 2000-05-01 Surrogatcellebaseret system og fremgangsmåde til at analysere aktiviteten for Hepatitis C-virus NS3-protease DK1177211T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13236099P 1999-05-04 1999-05-04
PCT/CA2000/000496 WO2000066623A2 (en) 1999-05-04 2000-05-01 Surrogate cell-based system and method for assaying the activity of hepatitis c virus ns3 protease

Publications (1)

Publication Number Publication Date
DK1177211T3 true DK1177211T3 (da) 2008-06-02

Family

ID=22453660

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00922396T DK1177211T3 (da) 1999-05-04 2000-05-01 Surrogatcellebaseret system og fremgangsmåde til at analysere aktiviteten for Hepatitis C-virus NS3-protease

Country Status (14)

Country Link
US (3) US20030162168A1 (da)
EP (1) EP1177211B1 (da)
JP (1) JP4782927B2 (da)
AT (1) ATE386746T1 (da)
AU (1) AU772386B2 (da)
CA (1) CA2365262C (da)
DE (1) DE60038113T2 (da)
DK (1) DK1177211T3 (da)
ES (1) ES2300262T3 (da)
HU (1) HUP0200945A3 (da)
IL (2) IL145376A0 (da)
MX (1) MXPA01011045A (da)
NZ (1) NZ515861A (da)
WO (1) WO2000066623A2 (da)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MXPA01011045A (es) * 1999-05-04 2002-07-22 Boehringer Ingelheim Ltd Sistema sustituto basado en celulas y metodo para evaluar la actividad de la proteasa ns3 de virus de la hepatitis c.
EP1196436A2 (en) * 1999-07-07 2002-04-17 Bristol-Myers Squibb Pharma Company Peptide boronic acid inhibitors of hepatitis c virus protease
US6986892B1 (en) 1999-11-24 2006-01-17 Chiron Corporation Immunogenic Hepatitis C virus non-structural polypeptides
CN1425027A (zh) * 1999-11-24 2003-06-18 希龙公司 新颖的hcv非结构多肽
US6921634B2 (en) * 1999-12-23 2005-07-26 Board Of Regents, The University Of Texas System Replication competent hepatitus C virus and methods of use
US6528252B2 (en) * 2000-02-25 2003-03-04 Biofocus Discovery Limited Methods for identifying a protease inhibitor
ES2287074T3 (es) 2000-12-22 2007-12-16 Boehringer Ingelheim (Canada) Ltd. Molecula de rna autorreplicante del virus de la hepatitis c.
DE10234418A1 (de) * 2002-07-29 2004-02-12 Bayer Ag Verfahren zur Untersuchung und Beurteilung von Virusenzymen im HTS
JP2007505603A (ja) * 2003-09-12 2007-03-15 ヴァーテックス ファーマシューティカルズ、 インコーポレイテッド プロテアーゼ活性および肝障害のための動物モデル
AU2004281780B2 (en) 2003-10-14 2009-03-19 F. Hoffmann-La Roche Ltd Macrocyclic carboxylic acids and acylsulfonamides as inhibitors of HCV replication
MX2008001166A (es) 2005-07-25 2008-03-18 Intermune Inc Nuevos inhibidores macrociclicos de la replicacion del virus de hepatitis c.
ATE493409T1 (de) 2005-10-11 2011-01-15 Intermune Inc Verbindungen und verfahren zur inhibierung der replikation des hepatitis-c-virus
US7705138B2 (en) 2005-11-11 2010-04-27 Vertex Pharmaceuticals Incorporated Hepatitis C virus variants
EP2392590A3 (en) 2005-11-11 2012-03-14 Vertex Pharmaceuticals Incorporated Hepatitis C virus variants
WO2013027217A1 (en) * 2011-08-22 2013-02-28 Ramot At Tel-Aviv University Ltd. Zymoxins and methods of using the same
CA2890277A1 (en) * 2012-11-07 2014-05-15 Gilead Sciences, Inc. Hcv genotype 6 replicons
US20210087548A1 (en) * 2017-07-24 2021-03-25 The Board Of Trustees Of The Leland Stanford Junior University Compositions and Methods for Inducing Protein Function
WO2022094463A1 (en) * 2020-11-02 2022-05-05 Regents Of The University Of Minnesota Live cell assay for protease inhibition

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ238681A (en) * 1990-06-25 1992-03-26 Univ Osaka Res Found Non-a, non-b hepatitis virus particles and their production
JP3269642B2 (ja) * 1990-06-25 2002-03-25 財団法人阪大微生物病研究会 非Ａ非Ｂ型肝炎ウイルス遺伝子ｃＤＮＡおよび抗原ポリペプチド
US5861267A (en) * 1995-05-01 1999-01-19 Vertex Pharmaceuticals Incorporated Methods, nucleotide sequences and host cells for assaying exogenous and endogenous protease activity
IT1277914B1 (it) * 1995-08-22 1997-11-12 Angeletti P Ist Richerche Bio Procedimento per produrre - in forma pura e in quantita' elevate - polipeptidi con l'attivita' proteolitica della proteasi ns3 di hcv, e
KR100209095B1 (ko) * 1996-06-28 1999-07-15 성재갑 C형 간염 바이러스의 프로테아제의 활성을 측정할 수 있는 c형 간염 대체 바이러스, 그 재조합 유전자 및 그 용도
US6280940B1 (en) * 1998-08-05 2001-08-28 Agouron Pharmaceuticals, Inc. Reporter gene system for use in cell-based assessment of inhibitors of the Hepatitis C virus protease
US6117639A (en) * 1998-08-31 2000-09-12 Vertex Pharmaceuticals Incorporated Fusion proteins, DNA molecules, vectors, and host cells useful for measuring protease activity
MXPA01011045A (es) * 1999-05-04 2002-07-22 Boehringer Ingelheim Ltd Sistema sustituto basado en celulas y metodo para evaluar la actividad de la proteasa ns3 de virus de la hepatitis c.

Also Published As

Publication number Publication date
IL145376A0 (en) 2002-06-30
JP4782927B2 (ja) 2011-09-28
US7276373B2 (en) 2007-10-02
ES2300262T3 (es) 2008-06-16
AU772386B2 (en) 2004-04-29
EP1177211A2 (en) 2002-02-06
JP2002542812A (ja) 2002-12-17
WO2000066623A2 (en) 2000-11-09
EP1177211B1 (en) 2008-02-20
DE60038113D1 (de) 2008-04-03
NZ515861A (en) 2004-08-27
WO2000066623A3 (en) 2001-03-22
US20030162169A1 (en) 2003-08-28
DE60038113T2 (de) 2009-02-12
MXPA01011045A (es) 2002-07-22
ATE386746T1 (de) 2008-03-15
US20030162168A1 (en) 2003-08-28
IL145376A (en) 2009-09-01
CA2365262C (en) 2008-06-10
AU4282500A (en) 2000-11-17
CA2365262A1 (en) 2000-11-09
US20050227226A1 (en) 2005-10-13
HUP0200945A3 (en) 2004-07-28
US7838207B2 (en) 2010-11-23
HUP0200945A2 (en) 2002-08-28

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