HUP0000486A2 - Rögzített enzim alkalmazása triglicerid olajok előállítására - Google Patents

Rögzített enzim alkalmazása triglicerid olajok előállítására

Info

Publication number
HUP0000486A2
HUP0000486A2 HU0000486A HUP0000486A HUP0000486A2 HU P0000486 A2 HUP0000486 A2 HU P0000486A2 HU 0000486 A HU0000486 A HU 0000486A HU P0000486 A HUP0000486 A HU P0000486A HU P0000486 A2 HUP0000486 A2 HU P0000486A2
Authority
HU
Hungary
Prior art keywords
enzyme
phase
aqueous phase
triglyceride oils
water
Prior art date
Application number
HU0000486A
Other languages
English (en)
Inventor
Jurgen Fabian
Johan Paul T. Geurtsen
Martin Roger Grote
Adrianus Rozendaal
Karel P. Van Putte
Original Assignee
Unilever N.V.,
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever N.V., filed Critical Unilever N.V.,
Publication of HUP0000486A2 publication Critical patent/HUP0000486A2/hu
Publication of HUP0000486A3 publication Critical patent/HUP0000486A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6454—Glycerides by esterification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)

Abstract

A találmány tárgya eljárás enzim rögzítésére, ami abban áll, hőgy a.az immőbilízálásra szőlgáló lipáz enzimet kiválasztják, b. főlytőnőshidrőfób fázisból és diszpergált vizes fázisból álló eműlziótkészítenek, amely vizes fázis őldatlan anyagőt tartalmaz, és emellettőldőtt állapőtban van jelen az enzim, és őlyan anyag, amely akövetkező lépés végrehajtása űtán az enzim hőrdőzójaként szőlgál, c. adiszpergált fázisból a vizet eltávőlítják, a víz eltávőlítását addigfőlytatják, míg ez a fázis szilárd enzimmel bevőnt részecskékkéalakűl. A hidrőfób fázis tartalmazhatja az enzim szűbsztrátjáűlszőlgáló anyagőkat, ezek közül egy vagy több, a trigliceridek,digliceridek, mőnőgliceridek, glicerin és zsírsavak körébe tartőzik.Megfelelő vizes fázis képezhető példáűl őlyan nyers lipáz fermentációsfőlyadékból, amely hőrdőzóanyagként alkalmas inaktivált biőmasszáttartalmaz. A rögzített enzim alkalmazásával végrehajtható eljárásőkkörébe tartőznak ehető triglicerid őlajők észter átrendezési,acidőlízis és savmentesítési eljárásai. Az eljárás igen kisenzimfőgyasztással jár, és gazdaságős. ŕ
HU0000486A 1996-12-19 1997-12-03 Immobilized enzyme and its use for the processing of triglyceride oils HUP0000486A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP96203644 1996-12-19
PCT/EP1997/006957 WO1998027199A1 (en) 1996-12-19 1997-12-03 Immobilized enzyme and its use for the processing of triglyceride oils

Publications (2)

Publication Number Publication Date
HUP0000486A2 true HUP0000486A2 (hu) 2000-06-28
HUP0000486A3 HUP0000486A3 (en) 2001-10-29

Family

ID=8224734

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0000486A HUP0000486A3 (en) 1996-12-19 1997-12-03 Immobilized enzyme and its use for the processing of triglyceride oils

Country Status (14)

Country Link
US (1) US6025171A (hu)
EP (1) EP0951535A1 (hu)
JP (1) JP4017082B2 (hu)
AU (1) AU741174B2 (hu)
BR (1) BR9713949A (hu)
CA (1) CA2275707A1 (hu)
CO (1) CO4650228A1 (hu)
CZ (1) CZ221899A3 (hu)
HU (1) HUP0000486A3 (hu)
PL (1) PL334336A1 (hu)
SK (1) SK80799A3 (hu)
TR (1) TR199901337T2 (hu)
WO (1) WO1998027199A1 (hu)
ZA (1) ZA9711159B (hu)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU720776B2 (en) * 1995-06-27 2000-06-08 Unilever Plc Immobilized enzyme and its use for the processing of triglyceride oils
ES2395898T3 (es) 2002-04-19 2013-02-15 Dsm Ip Assets B.V. Fosfolipasas, ácidos nucleicos que las codifican, y métodos para obtenerlas y usarlas
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
AU2003257211A1 (en) * 2002-08-09 2004-02-25 The Procter And Gamble Company A process for immobilizing an enzyme
EP1639108A2 (en) 2003-02-28 2006-03-29 McGILL UNIVERSITY Cell and enzyme compositions for modulating bile acids, cholesterol and triglycerides
CA3007908A1 (en) 2003-03-07 2005-04-14 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
DE102004019472A1 (de) * 2004-04-22 2005-11-17 Bayer Healthcare Ag Phenylacetamide
GB0600913D0 (en) * 2006-01-17 2006-02-22 Syngenta Ltd Improvements in or relating to organic compounds
WO2007092314A2 (en) 2006-02-02 2007-08-16 Verenium Corporation Esterases and related nucleic acids and methods
PT2057274E (pt) 2006-09-21 2014-03-13 Dsm Ip Assets Bv Fosfolípases, ácidos nucleicos que as codificam e métodos de preparação e utilização das mesmas
UA97127C2 (uk) * 2006-12-06 2012-01-10 Бандж Ойлз, Инк. Спосіб безперервної ферментативної обробки композиції, що містить ліпід, та система для його здійснення
US20100216209A1 (en) * 2007-07-31 2010-08-26 Eiji Iwaoka Immobilized lipase and method for producing the same
US8153391B2 (en) 2008-08-29 2012-04-10 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8357503B2 (en) 2008-08-29 2013-01-22 Bunge Oils, Inc. Hydrolases, nucleic acids encoding them and methods for making and using them
US8198062B2 (en) * 2008-08-29 2012-06-12 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
CN101381721B (zh) * 2008-09-26 2011-01-05 北京凯泰新世纪生物技术有限公司 一种固定化脂肪酶及其制备方法
FR2947564B1 (fr) 2009-07-06 2011-07-22 Univ Paris Curie Catalyseur enzymatique heterogene, procede de preparation et utilisation
UA109884C2 (uk) 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU540882B2 (en) * 1980-03-08 1984-12-06 Fuji Oil Company Limited Enzymatic transesterification of lipid and enzyme used therein
DK402583D0 (da) * 1983-09-05 1983-09-05 Novo Industri As Fremgangsmade til fremstilling af et immobiliseret lipasepraeparat og anvendelse deraf
US4940845A (en) * 1984-05-30 1990-07-10 Kao Corporation Esterification process of fats and oils and enzymatic preparation to use therein
DK399387D0 (da) * 1987-07-31 1987-07-31 Novo Industri As Immobiliseret lipase og dennes anvendelse
WO1989002916A1 (en) * 1987-09-28 1989-04-06 Novo-Nordisk A/S Method for immobilizing lipase
DE3854042T2 (de) * 1987-12-09 1995-11-16 Kao Corp., Tokio/Tokyo Immobilisiertes Enzym und Veresterung und Zwischenveresterung mit demselben.
FI96036C (fi) * 1988-08-24 1996-04-25 Allied Colloids Ltd Menetelmä partikkelien valmistamiseksi, jotka sisältävät biologisesti tuotetun aineen polymeerimateriaalimatriisissa
DK638688D0 (da) * 1988-11-16 1988-11-16 Novo Industri As Partikelformet immobiliseret lipase-praeparat, fremgangsmaade til fremstilling deraf og anvendelse deraf
GB8915299D0 (en) * 1989-07-04 1989-08-23 Cerestar Holding Bv Carbohydrate refining process and a supported enzyme for use therein
DE4027290C2 (de) * 1990-08-29 1995-03-23 Forschungszentrum Juelich Gmbh Verfahren zur Gewinnung von Enzymen aus enzymhaltigen Suspensionen
US5182201A (en) * 1990-10-26 1993-01-26 Uop Lipase immobilization without covalent bonding on an amphiphilic support containing lipophilic alkyl chains
DE4115938A1 (de) * 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
JP3194792B2 (ja) * 1992-06-12 2001-08-06 天野エンザイム株式会社 酵素を用いた脂肪族ポリエステルの分解法
DE69531538T2 (de) * 1994-02-21 2004-06-24 Novozymes A/S Verfahren zur herstellung einer immobilisierten enzympräparation und ihre verwendung
AU720776B2 (en) * 1995-06-27 2000-06-08 Unilever Plc Immobilized enzyme and its use for the processing of triglyceride oils

Also Published As

Publication number Publication date
CA2275707A1 (en) 1998-06-25
ZA9711159B (en) 1999-06-11
WO1998027199A1 (en) 1998-06-25
CO4650228A1 (es) 1998-09-03
AU741174B2 (en) 2001-11-22
PL334336A1 (en) 2000-02-28
EP0951535A1 (en) 1999-10-27
AU5662398A (en) 1998-07-15
HUP0000486A3 (en) 2001-10-29
US6025171A (en) 2000-02-15
JP4017082B2 (ja) 2007-12-05
SK80799A3 (en) 2000-03-13
BR9713949A (pt) 2000-03-21
CZ221899A3 (cs) 1999-11-17
TR199901337T2 (xx) 1999-10-21
JP2001506499A (ja) 2001-05-22

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