HUE034396T2 - Tenyésztõ tápközeg, eljárás Salmonella és E. coli tenyésztésére és eljárás Salmonella és E. coli kimutatására - Google Patents

Tenyésztõ tápközeg, eljárás Salmonella és E. coli tenyésztésére és eljárás Salmonella és E. coli kimutatására Download PDF

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HUE034396T2
HUE034396T2 HUE12811195A HUE12811195A HUE034396T2 HU E034396 T2 HUE034396 T2 HU E034396T2 HU E12811195 A HUE12811195 A HU E12811195A HU E12811195 A HUE12811195 A HU E12811195A HU E034396 T2 HUE034396 T2 HU E034396T2
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concentration
salmonella
medium
coli
ethanolamine
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HUE12811195A
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Gabriela Martinez
Renaud Tremblay
Janikim Larochelle
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Foodchek Systems Inc
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
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    • C12N2500/00—Specific components of cell culture medium
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    • C12Q2304/00—Chemical means of detecting microorganisms
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/879—Salmonella

Claims (8)

  1. TENYÉSZTŐ TÂPKÔZEG, ELJÁRÁS SALMONELLA ÉS R COU TENYÉSZTÉSÉRE Ê$ ELJÁRÁS SALMONELLA ÉS JÉL COU KIMUTATÁSÁRA Szabadalmi igénypontok I. Tenyésztő íápközeg, amely alkalmas Salmonéla ψρ cxMschmchto colt (E caE) kimutatására éleim iszer-miptábao vagy környezetből vett mintában, ahol a tenyésztő tápközeg a következőket tartalmazó tápanyagkeverék biológiailag hatásos koncentrációját tartalmazza: kaleíum-klorid 0,001-9,05 g/L. koncentrációban; monöbázisos kálium-foszfát 1-6 g/L koncentrációban; magnézium-szulfát 0*01 -0,5 g/L koncentrációban; náirium-kforid 0,1-1 g/L .koncentrációban; diházisos-nátrium-foszfát. 0,5-10 g/L koncentrációban; eiiröttisäv 0,1-1 g/L koneeturáetóban; ékiszlőkivonat 1-4 g/L koncentrációban; B1.2 vitamin 0,14,0 mg/'L koncentrációban; etanolamin 1,0-3,0 g/L koncentrációban; """-"""""vas-szulfát 0,01-0,05 g/L koneentrációban; és nátrium·-piruvát 0,5-2,0 g/L koncentrációban.
  2. 3, Λζ 1. igénypont szerinti tápközeg, ahol a tápanyagkeverék 3 g/L koncentrációban tartalmaz élesztőkivonaíot.
    3. Az I igénypont szerinti tápközeg, amelyben a kalcium-klorid 0,013 g/L, koncentrációban van jelen.
  3. 4. Az 1. igénypont szerinti tápközeg, amelyben a monobázisos kálium-foszfát 3 g/L. koncentrációban van jelen,
  4. 5. Az I. igénypont szerinti tápközeg, amelyben a magnézium-szulfát 0J2 g/L koncentrációban van jelen.
  5. 6. Az 1. igénypont, szerinti tápközeg, amelyben a nátrium-kloríd 0,5 g/L koncentrációban van jelen,
  6. 7. Az 1. igénypont szerinti tápközeg, amelyben a citromsav 0,54? g/L koneentrációban van jelem
  7. 8. Az 1-7. igénypontok bármelyike szerinti tápközeg, amelyben a B i2~v itatni η 0,5 mg/L koncentrációban van jelen; az eianolatnín 2,0 g/L koncén nációban van jelen; a vas-szulfát 0,019 g/L. koncentrációban van jelén; és a fiálrium-piruvát 1,0 g/L, koncentrációban van je len,
  8. 9. Eljárás Salmonella spp vagy Escherichia coli tenyésztésére éieirnLszerminiában vagy környezeti mintában, ahol az. eljárás tartalmazza a minta inkubáSásártak a lépést az 1-8, igénypontok bármelyike szerinti tápközegben, .10. A 9. igénypont szerinti eljárás, ahol a tápanyagközeg 3 g/L koncentrációban tartalmaz élesztökivonatot. ÍL A 9. vagy 10. igénypont szerinti eljárás, ahol a tápközeg tartalmaz. B12-vitamint 0,5 mg/L koncentrációban; etanolamini 2,0 g/L koncentrációban; vas-szül tatot 0,019 g/L, koncentrációban; és nátrium-piruvátot 1,0 g/L koncentrációban, 12. A 11. igénypont szerinti eljárás, ahol az Escherichia coli (£. coli) £. coli 0157, shigatoxint termelő E. coli (STEC) és etrierahemorrhagíás £. coil (EHEC). 13. A 11. igénypont szerinti eljárás, ahol a Salmonella spp Salmonella -typhimuriim és Salmonella unterica, 14. A 9-13:, igénypontok bármelyike szerinti eljárás, amely tartalmazza továbbá az inkubálásl lépés után Salmonella spp vagy E< eolil detektálásának a lépését, és ahol a detektálás polimeráz-láncrakeióvaj (PCR), íektinkötésen keresztül, egyszerű diffúzióval, laterális diffúzióval, antitestkötéssel, laterális áramlással, immunológiai detektálással, ELISA-val vagy átfolyásé s („flow-through") lépéssel történik.
HUE12811195A 2011-07-12 2012-07-10 Tenyésztõ tápközeg, eljárás Salmonella és E. coli tenyésztésére és eljárás Salmonella és E. coli kimutatására HUE034396T2 (hu)

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CN108285917A (zh) * 2017-12-26 2018-07-17 广州聚佰生物科技有限公司 同时检测大肠菌群和沙门氏菌的显色培养基及检测片

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