HRP20211562T1 - Molekularno otkrivanje/dijagnostika reagensa za tumor - Google Patents
Molekularno otkrivanje/dijagnostika reagensa za tumor Download PDFInfo
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- HRP20211562T1 HRP20211562T1 HRP20211562TT HRP20211562T HRP20211562T1 HR P20211562 T1 HRP20211562 T1 HR P20211562T1 HR P20211562T T HRP20211562T T HR P20211562TT HR P20211562 T HRP20211562 T HR P20211562T HR P20211562 T1 HRP20211562 T1 HR P20211562T1
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- detection
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- 238000001514 detection method Methods 0.000 title claims 29
- 239000003153 chemical reaction reagent Substances 0.000 title claims 19
- 206010028980 Neoplasm Diseases 0.000 title claims 10
- 238000003745 diagnosis Methods 0.000 title claims 2
- 239000000523 sample Substances 0.000 claims 12
- 230000003321 amplification Effects 0.000 claims 7
- 238000003199 nucleic acid amplification method Methods 0.000 claims 7
- 101150118392 sdc-2 gene Proteins 0.000 claims 7
- 201000002313 intestinal cancer Diseases 0.000 claims 5
- 230000011987 methylation Effects 0.000 claims 5
- 238000007069 methylation reaction Methods 0.000 claims 5
- 208000003200 Adenoma Diseases 0.000 claims 3
- 206010001233 Adenoma benign Diseases 0.000 claims 3
- 206010009944 Colon cancer Diseases 0.000 claims 3
- 208000001333 Colorectal Neoplasms Diseases 0.000 claims 3
- 201000003741 Gastrointestinal carcinoma Diseases 0.000 claims 3
- 210000003608 fece Anatomy 0.000 claims 3
- 108091029523 CpG island Proteins 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 2
- 238000009007 Diagnostic Kit Methods 0.000 claims 2
- 208000005016 Intestinal Neoplasms Diseases 0.000 claims 2
- 239000011324 bead Substances 0.000 claims 2
- 101000995332 Homo sapiens Protein NDRG4 Proteins 0.000 claims 1
- 101150042012 SEPTIN9 gene Proteins 0.000 claims 1
- 101150006966 bmp3 gene Proteins 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 239000000439 tumor marker Substances 0.000 claims 1
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- B03—SEPARATION OF SOLID MATERIALS USING LIQUIDS OR USING PNEUMATIC TABLES OR JIGS; MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C—MAGNETIC OR ELECTROSTATIC SEPARATION OF SOLID MATERIALS FROM SOLID MATERIALS OR FLUIDS; SEPARATION BY HIGH-VOLTAGE ELECTRIC FIELDS
- B03C1/00—Magnetic separation
- B03C1/005—Pretreatment specially adapted for magnetic separation
- B03C1/015—Pretreatment specially adapted for magnetic separation by chemical treatment imparting magnetic properties to the material to be separated, e.g. roasting, reduction, oxidation
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- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- Plant Pathology (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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Claims (14)
1. Reagens za otkrivanje metilacije gena SDC2, koji sadrži prajmere za amplifikaciju kako je prikazano u SEQ ID NO.3 i SEQ ID NO.4.
2. Reagens za molekularno otkrivanje/dijagnostiku tumora, naznačen time što uzima izmet kao uzorak za otkrivanje, i sadrži reagens za otkrivanje metilacije gena SDC2 prema zahtjevu 1.
3. Reagens za otkrivanje/dijagnostiku prema zahtjevu 2, naznačen time što nadalje sadrži reagense za hvatanje DNA.
4. Reagens za otkrivanje/dijagnostiku prema zahtjevu 3, naznačen time što su reagensi za hvatanje DNK magnetske kuglice za hvatanje.
5. Reagens za otkrivanje/dijagnostiku prema zahtjevu 4, naznačen time što magnetske kuglice za hvatanje sadrže hvatajuće sekvence za gen SDC2, poželjno sadrže hvatajuće sekvence za otok CpG gena SDC2.
6. Reagens za otkrivanje/dijagnostiku prema zahtjevu 5, naznačen time što su hvatajuće sekvence kako je prikazano u SEQ ID NO.1 ili SEQ ID NO.2.
7. Reagens za otkrivanje/dijagnostiku prema zahtjevu 2, naznačen time što je reagens za otkrivanje metilacije gena SDC2 reagens za otkrivanje metilacije za otok CpG.
8. Reagens za otkrivanje/dijagnostiku prema zahtjevu 2, naznačen time što nadalje sadrži sondu za otkrivanje; poželjno, sonda za otkrivanje je kako je prikazano u SEQ ID NO.25 ili SEQ ID NO.26, poželjno je sonda kao što je prikazano u SEQ ID NO.26.
9. Reagens za otkrivanje/dijagnostiku prema bilo kojem od zahtjeva 2-8, naznačen time što je tumor rak crijeva ili prekancerogeni adenom, i poželjno je da je rak crijeva kolorektalni rak.
10. Komplet za otkrivanje/dijagnostiku tumora, naznačen time što sadrži reagens za otkrivanje/dijagnostiku prema bilo kojem od zahtjeva 2 do 9; poželjno, tumor je rak crijeva ili prekancerogeni adenom; i još poželjnije, rak crijeva je kolorektalni rak.
11. Metoda otkrivanja tumora, naznačena time što se izmet koristi kao uzorak za otkrivanje; nakon što se uzorak za otkrivanje pomiješa s reagensom za otkrivanje/dijagnostiku prema bilo kojem od zahtjeva 2 do 9, mješavina se podvrgne ekstrakciji i amplifikaciji; i rezultat otkrivanja dobiva se prema rezultatu amplifikacije.
12. Metoda otkrivanja prema zahtjevu 11, naznačena time što je objekt amplifikacije tumorski marker koji je odabran između gena SDC2, gena NDRG4, gena BMP3 ili gena Septin9.
13. Postupak otkrivanja prema zahtjevu 11 ili 12, naznačen time što se rezultat otkrivanja dobiva usporedbom rezultata amplifikacije uzorka koji se želi otkriti s rezultatom amplifikacije normalnog uzorka; i ako rezultat amplifikacije uzorka koji se želi otkriti i normalnog uzorka imaju značajnu razliku ili iznimno značajnu razliku, davatelj uzorka predstavlja pozitivan rezultat.
14. Uporaba reagensa za otkrivanje metilacije gena SDC2 prema zahtjevu 1, reagensa za otkrivanje/dijagnostiku prema bilo kojem od zahtjeva 2 do 9, ili kompleta za otkrivanje/dijagnostiku tumora prema zahtjevu 10 za otkrivanje tumora, pri čemu se izmet koristi kao uzorak za otkrivanje; poželjno, tumor je rak crijeva ili prekancerogeni adenom; poželjno, tumor je kolorektalni rak.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511034264.XA CN105543354B (zh) | 2015-12-31 | 2015-12-31 | 一种肿瘤分子检测/诊断试剂 |
| EP16880940.8A EP3425055B1 (en) | 2015-12-31 | 2016-12-13 | Molecular detection/diagnosis reagent for tumor |
| PCT/CN2016/109622 WO2017114150A1 (zh) | 2015-12-31 | 2016-12-13 | 一种肿瘤分子检测/诊断试剂 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20211562T1 true HRP20211562T1 (hr) | 2022-01-07 |
Family
ID=55822911
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20211562TT HRP20211562T1 (hr) | 2015-12-31 | 2016-12-13 | Molekularno otkrivanje/dijagnostika reagensa za tumor |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US10876168B2 (hr) |
| EP (1) | EP3425055B1 (hr) |
| JP (1) | JP6609055B2 (hr) |
| KR (1) | KR102105260B1 (hr) |
| CN (1) | CN105543354B (hr) |
| AU (1) | AU2016383317B2 (hr) |
| CA (1) | CA3010143C (hr) |
| CY (1) | CY1124654T1 (hr) |
| DK (1) | DK3425055T3 (hr) |
| ES (1) | ES2893302T3 (hr) |
| HR (1) | HRP20211562T1 (hr) |
| HU (1) | HUE056315T2 (hr) |
| LT (1) | LT3425055T (hr) |
| MY (1) | MY195999A (hr) |
| PL (1) | PL3425055T3 (hr) |
| PT (1) | PT3425055T (hr) |
| RS (1) | RS62642B1 (hr) |
| SG (1) | SG11201805531UA (hr) |
| SI (1) | SI3425055T1 (hr) |
| WO (1) | WO2017114150A1 (hr) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543354B (zh) * | 2015-12-31 | 2019-07-26 | 广州市康立明生物科技有限责任公司 | 一种肿瘤分子检测/诊断试剂 |
| CN106893777B (zh) * | 2017-03-02 | 2021-04-30 | 武汉艾米森生命科技有限公司 | 用于检测结直肠癌相关基因多位点甲基化试剂盒及应用 |
| CN106947822B (zh) | 2017-04-12 | 2020-04-10 | 广州市康立明生物科技有限责任公司 | Itga4基因甲基化检测试剂、试剂盒及检测方法 |
| CN108642180A (zh) * | 2018-05-21 | 2018-10-12 | 上海锐翌生物科技有限公司 | 检测sdc2基因甲基化的方法和试剂盒 |
| CN110511997A (zh) * | 2018-05-22 | 2019-11-29 | 广州市康立明生物科技有限责任公司 | 肿瘤标志物、甲基化试剂、试剂盒及其应用 |
| CN108977543B (zh) * | 2018-08-06 | 2021-12-14 | 上海锐翌生物科技有限公司 | 一种基于联合检测sdc2和sfrp2基因甲基化水平的结直肠癌早期诊断试剂 |
| CN110964812B (zh) * | 2018-09-29 | 2021-10-22 | 广州康立明生物科技股份有限公司 | Hoxa9甲基化检测试剂在制备肺癌诊断试剂中的用途 |
| CN110964813B (zh) * | 2018-09-29 | 2021-11-16 | 广州康立明生物科技股份有限公司 | Hoxa7甲基化检测试剂在制备肺癌诊断试剂中的用途 |
| KR102280363B1 (ko) * | 2019-06-18 | 2021-07-22 | (주)지노믹트리 | Sdc2 유전자의 메틸화 검출방법 |
| CN110283911A (zh) * | 2019-06-28 | 2019-09-27 | 四川沃文特生物技术有限公司 | 用于粪便样本进行早期结直肠癌基因甲基化检测的引物对和探针及试剂盒 |
| CN110331142B (zh) * | 2019-07-11 | 2021-12-21 | 广州康立明生物科技股份有限公司 | 一种多基因联合检测试剂 |
| CN110317871B (zh) * | 2019-07-11 | 2022-06-07 | 广州康立明生物科技股份有限公司 | 一种基因标志物组合及其应用 |
| CN112301124A (zh) * | 2019-07-29 | 2021-02-02 | 人和未来生物科技（长沙）有限公司 | 用于结直肠癌和腺瘤辅助诊断的试剂盒及其使用方法 |
| CN110358836B (zh) * | 2019-07-31 | 2022-12-23 | 中山大学附属第六医院 | 筛查或诊断肿瘤的试剂组合、试剂盒和装置 |
| CN110373470A (zh) * | 2019-08-06 | 2019-10-25 | 阿吉安(福州)基因医学检验实验室有限公司 | 结直肠肿瘤特异性甲基化检测的引物、探针及试剂盒 |
| KR102261606B1 (ko) * | 2019-11-07 | 2021-06-07 | (주)지노믹트리 | 대장암 검출 방법 |
| CN114085905B (zh) * | 2020-08-24 | 2024-02-23 | 广州达健生物科技有限公司 | 用于检测结直肠癌的组合物及其试剂盒与应用 |
| CN114085904B (zh) * | 2020-08-24 | 2024-02-23 | 广州达健生物科技有限公司 | 结直肠癌基因甲基化检测引物探针组合及其试剂盒与应用 |
| CN112159846A (zh) * | 2020-09-22 | 2021-01-01 | 深圳市核子基因科技有限公司 | 组合物、试剂盒及其应用 |
| CN115537464B (zh) * | 2021-06-30 | 2023-06-20 | 武汉艾米森生命科技有限公司 | 一种结直肠癌或癌前病变的诊断或辅助诊断试剂、核酸组合、试剂盒及应用 |
| CN116334179A (zh) * | 2022-07-20 | 2023-06-27 | 圣湘生物科技股份有限公司 | 一种双特异性探针捕获磁珠及其制备方法和应用 |
| CN116987786A (zh) * | 2023-04-20 | 2023-11-03 | 广州奇辉生物科技有限公司 | 用于结直肠癌检测的靶基因组合、引物和探针以及试剂盒 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8216810B2 (en) * | 2004-04-27 | 2012-07-10 | The Trustees Of Columbia University In The City Of New York | Multiplex systems, methods, and kits for detecting and identifying nucleic acids |
| KR101142131B1 (ko) | 2009-11-05 | 2012-05-11 | (주)지노믹트리 | 장암 진단을 위한 장암 특이적 메틸화 마커 유전자의 메틸화 검출방법 |
| CN103314114B (zh) | 2010-09-13 | 2016-08-10 | 临床基因组学股份有限公司 | 结直肠癌的外遗传标记以及使用它们的诊断方法 |
| KR101569498B1 (ko) | 2012-11-07 | 2015-11-16 | (주)지노믹트리 | 위용종 및 위암 특이적 메틸화 마커 유전자를 이용한 위용종 및 위암의 검출방법 |
| WO2015023146A1 (ko) | 2013-08-14 | 2015-02-19 | (주)지노믹트리 | 전암병변의 검출방법 |
| EP2915884B1 (en) | 2014-03-03 | 2017-12-06 | Fundacio Institut d'Investigació Biomèdica de Girona Dr. Josep Trueta | Method for diagnosing colorectal cancer from a human feces sample by quantitive PCR |
| WO2015153283A1 (en) * | 2014-03-31 | 2015-10-08 | Mayo Foundation For Medical Education And Research | Detecting colorectal neoplasm |
| CN105543354B (zh) | 2015-12-31 | 2019-07-26 | 广州市康立明生物科技有限责任公司 | 一种肿瘤分子检测/诊断试剂 |
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Also Published As
| Publication number | Publication date |
|---|---|
| CY1124654T1 (el) | 2022-07-22 |
| EP3425055B1 (en) | 2021-07-07 |
| CN105543354A (zh) | 2016-05-04 |
| ES2893302T3 (es) | 2022-02-08 |
| SI3425055T1 (sl) | 2021-12-31 |
| RS62642B1 (sr) | 2021-12-31 |
| EP3425055A1 (en) | 2019-01-09 |
| US20190010557A1 (en) | 2019-01-10 |
| US10876168B2 (en) | 2020-12-29 |
| KR102105260B1 (ko) | 2020-04-27 |
| AU2016383317B2 (en) | 2019-11-14 |
| LT3425055T (lt) | 2021-10-25 |
| CA3010143C (en) | 2021-08-10 |
| WO2017114150A1 (zh) | 2017-07-06 |
| DK3425055T3 (da) | 2021-10-11 |
| CN105543354B (zh) | 2019-07-26 |
| CA3010143A1 (en) | 2017-07-06 |
| AU2016383317A1 (en) | 2018-07-19 |
| JP6609055B2 (ja) | 2019-11-20 |
| PL3425055T3 (pl) | 2022-01-10 |
| MY195999A (en) | 2023-02-27 |
| HUE056315T2 (hu) | 2022-02-28 |
| KR20180094090A (ko) | 2018-08-22 |
| EP3425055A4 (en) | 2019-08-28 |
| PT3425055T (pt) | 2021-10-06 |
| SG11201805531UA (en) | 2018-07-30 |
| JP2019501651A (ja) | 2019-01-24 |
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