HRP20191685T1 - Novi postupak - Google Patents
Novi postupak Download PDFInfo
- Publication number
- HRP20191685T1 HRP20191685T1 HRP20191685T HRP20191685T1 HR P20191685 T1 HRP20191685 T1 HR P20191685T1 HR P20191685 T HRP20191685 T HR P20191685T HR P20191685 T1 HRP20191685 T1 HR P20191685T1
- Authority
- HR
- Croatia
- Prior art keywords
- vol
- lps
- mixture
- extracting
- cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims 16
- 239000002158 endotoxin Substances 0.000 claims 16
- 229920006008 lipopolysaccharide Polymers 0.000 claims 16
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 7
- 239000000203 mixture Substances 0.000 claims 7
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims 6
- 230000001580 bacterial effect Effects 0.000 claims 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 5
- 238000000605 extraction Methods 0.000 claims 4
- 241001222774 Salmonella enterica subsp. enterica serovar Minnesota Species 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 2
- 241000588722 Escherichia Species 0.000 claims 1
- 241000588724 Escherichia coli Species 0.000 claims 1
- 241000607142 Salmonella Species 0.000 claims 1
- 238000005903 acid hydrolysis reaction Methods 0.000 claims 1
- 238000001704 evaporation Methods 0.000 claims 1
- 230000007062 hydrolysis Effects 0.000 claims 1
- 238000006460 hydrolysis reaction Methods 0.000 claims 1
- 239000003960 organic solvent Substances 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K3/00—Materials not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Materials Engineering (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Polymers & Plastics (AREA)
- Medicinal Chemistry (AREA)
- Sustainable Development (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Catalysts (AREA)
- Saccharide Compounds (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Extraction Or Liquid Replacement (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Claims (15)
1. Postupak ekstrahiranja lipopolisaharida (LPS) iz gram negativnih bakterijskih stanica, naznačen time što se sastoji u koraku: ekstrahiranja LPS iz stanica u smjesi za ekstrahiranje LPS koja sadrži kloroform, metanol i vodu, gdje je smjesa za ekstrahiranje LPS jednofazna, te gdje je količina vode u smjesi za ekstrahiranje LPS između 0,4 i 1,5% (vol./vol.), postotak metanola u smjesi za ekstrahiranje LPS je između 5% i 40% (vol./vol.), te gdje je postotak kloroforma u smjesi za ekstrahiranje LPS između 60% (vol./vol.) i 95% (vol./vol.).
2. Postupak u skladu s patentnim zahtjevom 1, naznačen time što je količina vode 0,8 do 1,2% (vol./vol.).
3. Postupak u skladu s patentnim zahtjevom 2, naznačen time što je količina vode 1% (vol./vol.).
4. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 3, naznačen time što je postotak metanola u smjesi za ekstrahiranje LPS između 10% i 30% (vol./vol.).
5. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 4, naznačen time što je postotak kloroforma u smjesi za ekstrahiranje LPS između 75% (vol./vol.) i 90% (vol./vol.).
6. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 5, naznačen time što se ekstrahiranje LPS provodi na temperaturi između otprilike 35 °C i otprilike 65 °C.
7. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 6, naznačen time što se LPS ekstrahiranje provodi na pH između 7,8 i 9.
8. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 7, naznačen time što se LPS ekstrahiranje provodi u trajanju od otprilike 0,5 do otprilike 20 sati.
9. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 8, naznačen time što dodatno uključuje korake:
i. ispiranja stanica otopinom etanola ili etanolom; i izborno
ii. ispiranja stanica po drugi put etanolom ili metanolom.
10. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 9, naznačen time što dodatno uključuje korak: isparavanja vode, alkohola i dodatnog organskog otapala iz otopine LPS, čime se dobije suhi ostatak LPS.
11. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 10, naznačen time što je bakterijska stanica ona duboko grubog mutantnog bakterijskog soja iz roda Salmonella ili Escherichia.
12. Postupak u skladu s patentnim zahtjevom 11, naznačen time što su bakterijske stanice one od bakterije Escherichia coli.
13. Postupak u skladu s patentnim zahtjevom 11, naznačen time što su bakterijske stanice one od bakterije Salmonella minnesota.
14. Postupak u skladu s patentnim zahtjevom 11, naznačen time što su bakterijske stanice one od bakterije Salmonella minnesota R595.
15. Postupak u skladu s bilo kojim od patentnih zahtjeva 1 do 14, naznačen time što dodatno uključuje korak: podvrgavanja LPS uzastopno kiseloj hidrolizi i bazičnoj hidrolizi, kako bi se dobilo 3D-MPL.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1008401.0A GB201008401D0 (en) | 2010-05-20 | 2010-05-20 | Novel process |
| EP11721756.2A EP2571902B1 (en) | 2010-05-20 | 2011-05-18 | Novel process |
| PCT/EP2011/058016 WO2011144645A1 (en) | 2010-05-20 | 2011-05-18 | Novel process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20191685T1 true HRP20191685T1 (hr) | 2019-12-13 |
Family
ID=42341031
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20191685 HRP20191685T1 (hr) | 2010-05-20 | 2019-09-17 | Novi postupak |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US20130066064A1 (hr) |
| EP (1) | EP2571902B1 (hr) |
| JP (2) | JP2013527289A (hr) |
| CN (1) | CN102933606B (hr) |
| BR (1) | BR112012028930B8 (hr) |
| CA (1) | CA2800368C (hr) |
| CY (1) | CY1122071T1 (hr) |
| DK (1) | DK2571902T3 (hr) |
| ES (1) | ES2748512T3 (hr) |
| GB (1) | GB201008401D0 (hr) |
| HR (1) | HRP20191685T1 (hr) |
| HU (1) | HUE047042T2 (hr) |
| LT (1) | LT2571902T (hr) |
| PL (1) | PL2571902T3 (hr) |
| PT (1) | PT2571902T (hr) |
| SI (1) | SI2571902T1 (hr) |
| WO (1) | WO2011144645A1 (hr) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11186652B2 (en) * | 2015-12-22 | 2021-11-30 | Glaxosmithkline Biologicals Sa | LPS extraction process |
| US10947073B2 (en) | 2017-10-03 | 2021-03-16 | Hewlett-Packard Development Company, L.P. | Velocity and torque based media motor control |
| JPWO2021060127A1 (hr) * | 2019-09-24 | 2021-04-01 | ||
| CN116554361B (zh) * | 2023-07-11 | 2023-11-03 | 江苏瑞科生物技术股份有限公司 | 用于提取脂多糖的抽提液和方法 |
| CN116585747B (zh) * | 2023-07-12 | 2023-10-27 | 江苏瑞科生物技术股份有限公司 | 一种脂多糖提取装置及提取方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4076589A (en) * | 1973-05-29 | 1978-02-28 | Schering Corporation | Process for the production of dihydroxyacetone |
| KR900007831B1 (ko) * | 1986-11-28 | 1990-10-20 | 더 리포조옴 캄파니, 인코포레이티드 | 인지질 조성물 |
| US4912094B1 (en) * | 1988-06-29 | 1994-02-15 | Ribi Immunochem Research Inc. | Modified lipopolysaccharides and process of preparation |
| CA2348928C (en) * | 1998-11-03 | 2010-01-26 | De Staat Der Nederlanden, Vertegenwoordigd Door De Minister Van Welzijn,Volksgezondheid En Cultuur | Lps with reduced toxicity from genetically modified gram negative bacteria |
| DE10013539B4 (de) * | 2000-03-20 | 2006-07-13 | Pharma-Zentrale Gmbh | Lipopolysaccharide aus Escherichia coli, Verfahren zu deren Herstellung und deren Verwendungen |
| US20030031684A1 (en) | 2001-03-30 | 2003-02-13 | Corixa Corporation | Methods for the production of 3-O-deactivated-4'-monophosphoryl lipid a (3D-MLA) |
| JP3793792B2 (ja) * | 2003-06-25 | 2006-07-05 | 群馬県 | スフィンゴ脂質および／またはスフィンゴ糖脂質含有物の製造方法 |
| JP4308714B2 (ja) * | 2004-06-02 | 2009-08-05 | 日新製糖株式会社 | 天然物由来グリセロ糖脂質の製造方法 |
| WO2007106073A2 (en) * | 2006-03-02 | 2007-09-20 | University Of Massachusetts | Modified pathogens for use as vaccines |
-
2010
- 2010-05-18 US US13/698,937 patent/US20130066064A1/en not_active Abandoned
- 2010-05-20 GB GBGB1008401.0A patent/GB201008401D0/en not_active Ceased
-
2011
- 2011-05-18 PT PT117217562T patent/PT2571902T/pt unknown
- 2011-05-18 SI SI201131789T patent/SI2571902T1/sl unknown
- 2011-05-18 BR BR112012028930A patent/BR112012028930B8/pt active IP Right Grant
- 2011-05-18 EP EP11721756.2A patent/EP2571902B1/en active Active
- 2011-05-18 JP JP2013510607A patent/JP2013527289A/ja active Pending
- 2011-05-18 CA CA2800368A patent/CA2800368C/en active Active
- 2011-05-18 HU HUE11721756A patent/HUE047042T2/hu unknown
- 2011-05-18 ES ES11721756T patent/ES2748512T3/es active Active
- 2011-05-18 WO PCT/EP2011/058016 patent/WO2011144645A1/en active Application Filing
- 2011-05-18 LT LTEP11721756.2T patent/LT2571902T/lt unknown
- 2011-05-18 DK DK11721756.2T patent/DK2571902T3/da active
- 2011-05-18 CN CN201180025064.1A patent/CN102933606B/zh active Active
- 2011-05-18 PL PL11721756T patent/PL2571902T3/pl unknown
-
2015
- 2015-01-08 US US14/592,894 patent/US9499639B2/en active Active
-
2016
- 2016-03-04 JP JP2016041783A patent/JP6271619B2/ja active Active
-
2019
- 2019-09-17 HR HRP20191685 patent/HRP20191685T1/hr unknown
- 2019-09-24 CY CY20191101006T patent/CY1122071T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP6271619B2 (ja) | 2018-01-31 |
| JP2016174601A (ja) | 2016-10-06 |
| CA2800368C (en) | 2016-08-23 |
| ES2748512T3 (es) | 2020-03-17 |
| SI2571902T1 (sl) | 2019-11-29 |
| CY1122071T1 (el) | 2020-11-25 |
| CA2800368A1 (en) | 2011-11-24 |
| JP2013527289A (ja) | 2013-06-27 |
| BR112012028930B8 (pt) | 2021-04-13 |
| BR112012028930B1 (pt) | 2020-12-15 |
| US20130066064A1 (en) | 2013-03-14 |
| EP2571902A1 (en) | 2013-03-27 |
| US9499639B2 (en) | 2016-11-22 |
| DK2571902T3 (da) | 2019-10-07 |
| HUE047042T2 (hu) | 2020-04-28 |
| PL2571902T3 (pl) | 2019-12-31 |
| LT2571902T (lt) | 2019-10-10 |
| CN102933606B (zh) | 2015-09-02 |
| PT2571902T (pt) | 2019-10-24 |
| WO2011144645A1 (en) | 2011-11-24 |
| US20150126727A1 (en) | 2015-05-07 |
| GB201008401D0 (en) | 2010-07-07 |
| CN102933606A (zh) | 2013-02-13 |
| EP2571902B1 (en) | 2019-07-10 |
| BR112012028930A2 (pt) | 2016-07-26 |
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