CN102858375A - 载有干扰核糖核酸的细胞微粒子、其制备方法及其应用 - Google Patents

载有干扰核糖核酸的细胞微粒子、其制备方法及其应用 Download PDF

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CN102858375A
CN102858375A CN2010800662810A CN201080066281A CN102858375A CN 102858375 A CN102858375 A CN 102858375A CN 2010800662810 A CN2010800662810 A CN 2010800662810A CN 201080066281 A CN201080066281 A CN 201080066281A CN 102858375 A CN102858375 A CN 102858375A
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small interfering
interfering rnas
microvesicles
preparation methods
containing small
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CN102858375B (zh
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张辰宇
曾科
刘丹青
张玉婧
顾宏伟
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Jiangsu Micromedmark Biotech Co Ltd
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Abstract

含有干扰核糖核酸的细胞微粒子、其制备方法及其应用。还提供包括含有干扰核糖核酸的药物组合和试剂盒。含有干扰核糖核酸的细胞微粒子以及包括该细胞微粒子的药物组合和试剂盒可用于研究干扰核糖核酸对受体细胞的影响作用。由于含有干扰核糖核酸的细胞微粒子能够稳定、高效、特异性地输送干扰核糖核酸,因而可用于治疗相关疾病。

Description

PCT国内申请,说明书已公开。

Claims (1)

  1. PCT国内申请,权利要求书已公开。
CN201080066281.0A 2010-05-26 2010-05-26 载有干扰核糖核酸的细胞微粒子、其制备方法及其应用 Active CN102858375B (zh)

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Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9856477B2 (en) * 2012-11-13 2018-01-02 Codiak Biosciences, Inc. Delivery of therapeutic agent
EP2971010B1 (en) 2013-03-14 2020-06-10 ModernaTX, Inc. Formulation and delivery of modified nucleoside, nucleotide, and nucleic acid compositions
US20160194368A1 (en) 2013-09-03 2016-07-07 Moderna Therapeutics, Inc. Circular polynucleotides
US20160194625A1 (en) 2013-09-03 2016-07-07 Moderna Therapeutics, Inc. Chimeric polynucleotides
CA2926218A1 (en) 2013-10-03 2015-04-09 Moderna Therapeutics, Inc. Polynucleotides encoding low density lipoprotein receptor
WO2015186013A1 (en) 2014-06-04 2015-12-10 Capsugel Belgium Nv Stable dosage form articles for oral administration
CA2955250A1 (en) 2014-07-16 2016-01-21 Moderna Therapeutics, Inc. Chimeric polynucleotides
WO2016014846A1 (en) 2014-07-23 2016-01-28 Moderna Therapeutics, Inc. Modified polynucleotides for the production of intrabodies
MX2017015962A (es) 2015-06-10 2018-07-06 Univ Texas Uso de exosomas para el tratamiento de enfermedades.
LT3394093T (lt) 2015-12-23 2022-04-25 Modernatx, Inc. Ox40 ligandus koduojančių polinukleotidų naudojimo būdai
MA43587A (fr) 2016-01-10 2018-11-14 Modernatx Inc Arnm thérapeutiques codant pour des anticorps anti-ctla-4
US20190216857A1 (en) * 2016-09-09 2019-07-18 Cornell University Delivery of nucleic acids, proteins and small molecules in vitreous vesicular bodies
JP7285220B2 (ja) 2017-05-18 2023-06-01 モデルナティエックス インコーポレイテッド 連結したインターロイキン-12(il12)ポリペプチドをコードするポリヌクレオチドを含む脂質ナノ粒子
WO2023156532A1 (en) * 2022-02-18 2023-08-24 Servicio Andaluz De Salud ISOLATED PLASMA MEMBRANE-DERIVED VESICLES (PMdV) FOR USE IN THE TREATMENT OF VIRAL INFECTIONS

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070917A2 (en) * 2002-02-20 2003-08-28 Sirna Therapeutics, Inc. Rna interference mediated inhibition of myc and myb genes or genes of their respective pathways
CN101386848A (zh) * 2008-08-12 2009-03-18 南京大学 细胞微粒子所载微小核糖核酸及其制备研究方法和应用
CN101432432A (zh) * 2006-05-03 2009-05-13 扬·奥洛夫·洛特温尔 核酸向细胞的胞外体转移
WO2009100029A1 (en) * 2008-02-01 2009-08-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
WO2009147519A1 (en) * 2008-06-06 2009-12-10 Centre National De La Recherche Scientifique - Cnrs- Use of endo-lysosomal system and secreted vesicles (exosome-like) in treatments and diagnostics based on small rna and experimental study of small rna

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040241176A1 (en) 2000-04-27 2004-12-02 Ap Cells. Inc. Method of producing membrane vesicles
US20030124513A1 (en) 2001-05-29 2003-07-03 Mcswiggen James Enzymatic nucleic acid treatment of diseases or conditions related to levels of HIV
WO2005032561A1 (ja) 2003-10-03 2005-04-14 Riken HIV−Vprの機能に関する発明
CA2609260A1 (en) 2004-06-02 2005-12-22 Sourcepharm, Inc. Rna-containing microvesicles and methods therefor
CN1948475B (zh) 2004-10-13 2010-12-08 厦门大学 可用于治疗艾滋病的重组表达载体、经改造的造血干细胞和方法
US9085778B2 (en) 2006-05-03 2015-07-21 VL27, Inc. Exosome transfer of nucleic acids to cells
CA2756670A1 (en) 2009-03-26 2010-09-30 The Regents Of The University Of California Mesenchymal stem cells producing inhibitory rna for disease modification
CN101869715A (zh) 2010-05-26 2010-10-27 江苏命码生物科技有限公司 载有干扰核糖核酸的细胞微粒子、其制备方法及其应用

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070917A2 (en) * 2002-02-20 2003-08-28 Sirna Therapeutics, Inc. Rna interference mediated inhibition of myc and myb genes or genes of their respective pathways
CN101432432A (zh) * 2006-05-03 2009-05-13 扬·奥洛夫·洛特温尔 核酸向细胞的胞外体转移
WO2009100029A1 (en) * 2008-02-01 2009-08-13 The General Hospital Corporation Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions
WO2009147519A1 (en) * 2008-06-06 2009-12-10 Centre National De La Recherche Scientifique - Cnrs- Use of endo-lysosomal system and secreted vesicles (exosome-like) in treatments and diagnostics based on small rna and experimental study of small rna
CN101386848A (zh) * 2008-08-12 2009-03-18 南京大学 细胞微粒子所载微小核糖核酸及其制备研究方法和应用

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
AHARON A等: "Monocyte-derived microparticles and exosomes induce procoagulant and apoptotic effects on endothelial cells", <THROMB HAEMOST> *
NOBUYOSHI KOSAKA等: "Secretory Mechanisms and Intercellular Transfer of MicroRNAs in Living Cells", <THE JOURNAL OF BIOLOGICAL CHEMISTRY> *
朱磊 等: "Exosomes 在肿瘤免疫方面的研究进展", 《中国生物制品学杂志》 *

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