CN102858375A - 载有干扰核糖核酸的细胞微粒子、其制备方法及其应用 - Google Patents
载有干扰核糖核酸的细胞微粒子、其制备方法及其应用 Download PDFInfo
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Abstract
含有干扰核糖核酸的细胞微粒子、其制备方法及其应用。还提供包括含有干扰核糖核酸的药物组合和试剂盒。含有干扰核糖核酸的细胞微粒子以及包括该细胞微粒子的药物组合和试剂盒可用于研究干扰核糖核酸对受体细胞的影响作用。由于含有干扰核糖核酸的细胞微粒子能够稳定、高效、特异性地输送干扰核糖核酸,因而可用于治疗相关疾病。
Description
PCT国内申请,说明书已公开。
Claims (1)
- PCT国内申请,权利要求书已公开。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/CN2010/073262 WO2011147086A1 (zh) | 2010-05-26 | 2010-05-26 | 载有干扰核糖核酸的细胞微粒子、其制备方法及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN102858375A true CN102858375A (zh) | 2013-01-02 |
CN102858375B CN102858375B (zh) | 2016-08-10 |
Family
ID=45003208
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201080066281.0A Active CN102858375B (zh) | 2010-05-26 | 2010-05-26 | 载有干扰核糖核酸的细胞微粒子、其制备方法及其应用 |
Country Status (3)
Country | Link |
---|---|
US (1) | US9376679B2 (zh) |
CN (1) | CN102858375B (zh) |
WO (1) | WO2011147086A1 (zh) |
Families Citing this family (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9856477B2 (en) * | 2012-11-13 | 2018-01-02 | Codiak Biosciences, Inc. | Delivery of therapeutic agent |
EP2971010B1 (en) | 2013-03-14 | 2020-06-10 | ModernaTX, Inc. | Formulation and delivery of modified nucleoside, nucleotide, and nucleic acid compositions |
US20160194368A1 (en) | 2013-09-03 | 2016-07-07 | Moderna Therapeutics, Inc. | Circular polynucleotides |
US20160194625A1 (en) | 2013-09-03 | 2016-07-07 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
CA2926218A1 (en) | 2013-10-03 | 2015-04-09 | Moderna Therapeutics, Inc. | Polynucleotides encoding low density lipoprotein receptor |
WO2015186013A1 (en) | 2014-06-04 | 2015-12-10 | Capsugel Belgium Nv | Stable dosage form articles for oral administration |
CA2955250A1 (en) | 2014-07-16 | 2016-01-21 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
WO2016014846A1 (en) | 2014-07-23 | 2016-01-28 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of intrabodies |
MX2017015962A (es) | 2015-06-10 | 2018-07-06 | Univ Texas | Uso de exosomas para el tratamiento de enfermedades. |
LT3394093T (lt) | 2015-12-23 | 2022-04-25 | Modernatx, Inc. | Ox40 ligandus koduojančių polinukleotidų naudojimo būdai |
MA43587A (fr) | 2016-01-10 | 2018-11-14 | Modernatx Inc | Arnm thérapeutiques codant pour des anticorps anti-ctla-4 |
US20190216857A1 (en) * | 2016-09-09 | 2019-07-18 | Cornell University | Delivery of nucleic acids, proteins and small molecules in vitreous vesicular bodies |
JP7285220B2 (ja) | 2017-05-18 | 2023-06-01 | モデルナティエックス インコーポレイテッド | 連結したインターロイキン-12(il12)ポリペプチドをコードするポリヌクレオチドを含む脂質ナノ粒子 |
WO2023156532A1 (en) * | 2022-02-18 | 2023-08-24 | Servicio Andaluz De Salud | ISOLATED PLASMA MEMBRANE-DERIVED VESICLES (PMdV) FOR USE IN THE TREATMENT OF VIRAL INFECTIONS |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003070917A2 (en) * | 2002-02-20 | 2003-08-28 | Sirna Therapeutics, Inc. | Rna interference mediated inhibition of myc and myb genes or genes of their respective pathways |
CN101386848A (zh) * | 2008-08-12 | 2009-03-18 | 南京大学 | 细胞微粒子所载微小核糖核酸及其制备研究方法和应用 |
CN101432432A (zh) * | 2006-05-03 | 2009-05-13 | 扬·奥洛夫·洛特温尔 | 核酸向细胞的胞外体转移 |
WO2009100029A1 (en) * | 2008-02-01 | 2009-08-13 | The General Hospital Corporation | Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions |
WO2009147519A1 (en) * | 2008-06-06 | 2009-12-10 | Centre National De La Recherche Scientifique - Cnrs- | Use of endo-lysosomal system and secreted vesicles (exosome-like) in treatments and diagnostics based on small rna and experimental study of small rna |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040241176A1 (en) | 2000-04-27 | 2004-12-02 | Ap Cells. Inc. | Method of producing membrane vesicles |
US20030124513A1 (en) | 2001-05-29 | 2003-07-03 | Mcswiggen James | Enzymatic nucleic acid treatment of diseases or conditions related to levels of HIV |
WO2005032561A1 (ja) | 2003-10-03 | 2005-04-14 | Riken | HIV−Vprの機能に関する発明 |
CA2609260A1 (en) | 2004-06-02 | 2005-12-22 | Sourcepharm, Inc. | Rna-containing microvesicles and methods therefor |
CN1948475B (zh) | 2004-10-13 | 2010-12-08 | 厦门大学 | 可用于治疗艾滋病的重组表达载体、经改造的造血干细胞和方法 |
US9085778B2 (en) | 2006-05-03 | 2015-07-21 | VL27, Inc. | Exosome transfer of nucleic acids to cells |
CA2756670A1 (en) | 2009-03-26 | 2010-09-30 | The Regents Of The University Of California | Mesenchymal stem cells producing inhibitory rna for disease modification |
CN101869715A (zh) | 2010-05-26 | 2010-10-27 | 江苏命码生物科技有限公司 | 载有干扰核糖核酸的细胞微粒子、其制备方法及其应用 |
-
2010
- 2010-05-26 CN CN201080066281.0A patent/CN102858375B/zh active Active
- 2010-05-26 US US13/700,067 patent/US9376679B2/en active Active
- 2010-05-26 WO PCT/CN2010/073262 patent/WO2011147086A1/zh active Application Filing
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003070917A2 (en) * | 2002-02-20 | 2003-08-28 | Sirna Therapeutics, Inc. | Rna interference mediated inhibition of myc and myb genes or genes of their respective pathways |
CN101432432A (zh) * | 2006-05-03 | 2009-05-13 | 扬·奥洛夫·洛特温尔 | 核酸向细胞的胞外体转移 |
WO2009100029A1 (en) * | 2008-02-01 | 2009-08-13 | The General Hospital Corporation | Use of microvesicles in diagnosis, prognosis and treatment of medical diseases and conditions |
WO2009147519A1 (en) * | 2008-06-06 | 2009-12-10 | Centre National De La Recherche Scientifique - Cnrs- | Use of endo-lysosomal system and secreted vesicles (exosome-like) in treatments and diagnostics based on small rna and experimental study of small rna |
CN101386848A (zh) * | 2008-08-12 | 2009-03-18 | 南京大学 | 细胞微粒子所载微小核糖核酸及其制备研究方法和应用 |
Non-Patent Citations (3)
Title |
---|
AHARON A等: "Monocyte-derived microparticles and exosomes induce procoagulant and apoptotic effects on endothelial cells", <THROMB HAEMOST> * |
NOBUYOSHI KOSAKA等: "Secretory Mechanisms and Intercellular Transfer of MicroRNAs in Living Cells", <THE JOURNAL OF BIOLOGICAL CHEMISTRY> * |
朱磊 等: "Exosomes 在肿瘤免疫方面的研究进展", 《中国生物制品学杂志》 * |
Also Published As
Publication number | Publication date |
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US20130209544A1 (en) | 2013-08-15 |
US20140302119A2 (en) | 2014-10-09 |
WO2011147086A1 (zh) | 2011-12-01 |
US9376679B2 (en) | 2016-06-28 |
CN102858375B (zh) | 2016-08-10 |
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