WO2005032561A1 - HIV−Vprの機能に関する発明 - Google Patents
HIV−Vprの機能に関する発明 Download PDFInfo
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- WO2005032561A1 WO2005032561A1 PCT/JP2004/014595 JP2004014595W WO2005032561A1 WO 2005032561 A1 WO2005032561 A1 WO 2005032561A1 JP 2004014595 W JP2004014595 W JP 2004014595W WO 2005032561 A1 WO2005032561 A1 WO 2005032561A1
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- hiv
- mrna
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- vpr protein
- vpr
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12—Antivirals
- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56988—HIV or HTLV
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16311—Human Immunodeficiency Virus, HIV concerning HIV regulatory proteins
- C12N2740/16322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
- G01N2333/08—RNA viruses
- G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
- G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
- G01N2333/16—HIV-1, HIV-2
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2500/00—Screening for compounds of potential therapeutic value
Definitions
- the present invention relates to a method for screening an anti-HIV-1 drug based on the function of a Vpr protein, and a pharmaceutical composition for treating HIV-1 infection.
- AIDS Acquired immunodeficiency syndrome
- HIV-1 human immunodeficiency virus
- the particle structure of HIV-1 is a spherical shape of lOOnm, and mainly contains RNA, which is the main body of the gene, and enzymes that act on viral replication, reverse transcriptase, integrase, and proteinase. So far, inhibitors targeting these enzymes have been developed as anti-HIV drugs.
- combination therapy HAART
- HIV-1 has the property that its reverse transcriptase has no mutation repair ability, so that its gene is likely to be mutated. It has become a problem. There is a strong need for urgent development of new AIDS treatments to overcome this problem.
- HIV-1 is a member of the retrovirus, which is characterized by having accessory genes such as nef, vif, vpr, and vpu in addition to structural genes such as gag, pol, and env, and regulatory genes. Has the most complex structures and functions. In HIV-1, more than 30 diverse viral mRNAs are synthesized from a single 9-kb genome. This diversity has been shown to be caused by differences in splicing patterns (J. Virology, p. 6365-6378, 1993). These diverse splicing patterns control the expression of viral proteins and are thought to play a major role in the viral life cycle. [0005] HIV-1 Vpr protein is an accessory protein abundantly contained in virus particles.
- Vpr protein which has the function of arresting the cell cycle of the host cell at the G2 phase, is a key to the development of AIDS.
- Vpr protein increases the transcriptional activity from the HIV-1 LTR, but it is unknown how it affects the synthesis of viral mRNA.
- the present inventors isolated the intracellular factor that binds to the Vpr protein by the yeast two-hybrid method.
- PJ biology As a result, spliceosome—associated protein 14 5 (SAP145) He saw that they were combined (50th Annual Meeting of the Japanese Virology Society 'General Assembly, Program' Abstracts, p. 108, October 16-18, 2002). The researchers found that the Vpr protein and SAP145 also interacted in cells and were partially co-localized in the nucleus. SAP145 was a constituent of splicing factor 3b contained in U2s nRNP in spliceosomes.
- the group of the present inventors presumed that the Vpr protein is involved in splicing, and analyzed the effect of the Vpr protein on the splicing of ⁇ -globin2 pre-mRNA.
- ⁇ -globin2 pre-mRNA which has three exons and two introns surrounded by it, was selected as a model for analyzing the effect on splicing.
- the Vpr protein has an activity to inhibit the splicing of intron 1 of ⁇ -globin2 pre-mRNA (the 50th Annual Meeting of the Japanese Society for Virology 'General Assembly, Program' Abstracts, p. 108, October 16-18, 2002).
- Vpr protein splicing inhibition by Vpr protein may have sequence specificity.
- the Vpr protein It is still unclear how the effect of inhibition of pricing has to do with the replication and proliferation of HIV-1! /, Na! / ,.
- Non-Patent Document 1 Virology, 6365-6378, 1993
- Non-patent document 2 Virology, 263: 313-322, 1999
- Non-Patent Document 3 Virology, 276: 323-322, 2000
- Non-patent document 4 Nat.Med., 4: 65-71, 1998
- Non-Patent Document 5 The 50th Annual Meeting of the Japanese Society for Virology 'General Assembly, Program' Abstracts, p. 108, October 16-18, 2002
- Non-Patent Document 6 Nucleic Acid Res., 29, 464-478, 2001
- Non-Patent Document 7 Adachi et al., J. Virology, 59 (2): 284-291, 1986
- Non-Patent Document 8 Nishino et al., Biochem. Biophys. Res. Commun., 232 (2): 550-
- Non-Patent Document 9 Annu.Rev. Microbiol., 52: 491-532, 1998
- Non-Patent Document 10 Virology, 6365-6378, 1993
- An object of the present invention is to provide a pharmaceutical composition for suppressing the proliferation of HIV-1. Another object of the present invention is to provide a method for screening a substance for suppressing the proliferation of HIV-1.
- the pre-mRNA generated from the HIV-1 genome is roughly classified into a 9 kb form, a 4 kb form, and a 2 kb form (sometimes referred to as a 1.8 kb form, but described herein as a 2 kb form).
- the 9 kb form pre-mRNA is characterized in that HIV-1 genomic RNA (SEQ ID NO: 1) has not been spliced.
- the 4 kb form pre-mRNA is characterized by being HIV-1 genomic RNA (SEQ ID NO: 1) strongly spliced. 2kb four The pre-mRNA is characterized in that HIV-1 genomic RNA (SEQ ID NO: 1) has been spliced twice or more. These 9 kb, 4 kb and 2 kb forms of pre-mRNA form a total of more than 30 types of mature mRNAs, which are used for the expression of HIV-1 protein.
- a plurality of mature mRNAs can be obtained from a 2 kb form of pre-mRNA, but in the present specification, a group of mature mRNAs derived from a 2 kb form of pre-mRNA is simply referred to as a “2 kb form of mRNA group”. Notation. Similarly, mature mRNAs derived from the 9 kb form of pre-mRNA are referred to as “9 kb form mRNAs”, and mature mRNAs derived from the 4 kb form of pre-mRNA are referred to as “4 kb form mRNAs”. Notation.
- virus-derived proteins In the HIV-1 life cycle, in the early stage of infection, virus-derived proteins also express the ability to express 2 kb forms of mRNAs. In the late stage of infection, proteins are expressed from 4 kb and 9 kb forms of mRNAs. Become.
- the proteins encoded by the 4kb and 9kb forms of mRNAs contain the structural proteins that make up HIV-1 particles, and their expression leads to viral replication and proliferation (see Figure 1).
- the present inventors found that in the presence of the Vpr protein, the amount of the mRNA group of the 4 kb form increased and the amount of the mRNA group of the 2 kb form decreased (see FIG. 2).
- the present invention provides a method for screening a substance that suppresses the growth of HIV-1, comprising the following steps:
- transfected cells in the presence of the candidate substance for 24 to 72 hours, preferably Incubate for 48 hours or 72 hours (this time may be determined by a person skilled in the art according to the cells used and the culture conditions);
- the candidate is a substance that inhibits the growth of HIV-1.
- a method that includes: A vector containing a mutated HIV-1 genome that exhibits the same expression pattern as the wild-type HIV-1 gene and is mutated so as not to express the Vpr protein is used.
- Mutations that do not express the Vpr protein include (a) mutations introduced into the start codon of the vpr gene and mutations introduced into the promoter, which are intended to prevent the expression of the protein encoded by the vpr gene. And (b) a mutation introduced into the vpr gene itself, which aims to cause the resulting mutant Vpr protein to lose the activity of the Vpr protein.
- the mutation that does not express the Vpr protein is a mutation that introduces a start codon of the vpr gene.
- a vector containing a mutant HIV-1 genome that exhibits a similar expression pattern to the wild-type HIV-1 gene and does not express the Vpr protein is the HIV-1 cDNA NL432 (sequence). No. 2; GenBank accession number M19921), a mutation was introduced into the start codon of the vpr gene (nucleotides 5559-5561 in SEQ ID NO: 2) to prevent the expression of the Vpr protein, thereby mutating the HIV-1 genome.
- This is a vector containing cDNA.
- the vector containing the mutant HIV-1 genome is a part of the Gag-Pol region of the HIV-1 genome (SEQ ID NO: 2; GenBank accession number M19921 1) in the HIV-1 cDNA NL432 (SEQ ID NO: 2).
- a vector containing genomic cDNA is a part of the Gag-Pol region of the HIV-1 genome (SEQ ID NO: 2; GenBank accession number M19921 1) in the HIV-1 cDNA NL432 (SEQ ID NO: 2).
- the deletion of a part of the Gag-Pol region of the HIV-1 genome is performed so that the virus gene can be safely handled even in a normal laboratory.
- This mutant HIV-1 genomic cDNA has been reported to exhibit almost the same mRNA expression pattern as the wild-type HIV-1 gene, because the virus loses its ability to infect and amplify the virus (N ucleic Acid Res., 29, 464-478, 2001).
- the screening method of the present invention includes a mutant HIV-1 genomic that exhibits the same expression pattern as the wild-type HIV-1 gene and is mutated so as not to express the Vpr protein.
- the vector can be prepared, for example, as follows.
- the expression pattern similar to that of the wild-type HIV-1 gene means that a transcription and splicing reaction occurs as in the case of the wild-type HIV-1 gene, and that the expression pattern is similar to that of the wild-type HIV-1 gene. It means showing the expression pattern of HIV-1 mRNA.
- HIV-1 cDNA NL432 (SEQ ID NO: 2; GenBank accession number M19921)
- a mutation was introduced into the start codon of the vpr gene (nucleotides 5559-5561 in SEQ ID NO: 2) to prevent expression of the Vpr protein.
- the mutated HIV-1 genomic cDNA (SEQ ID NO: 3) is treated with restriction enzymes PflMI and EcoRI, and the treated mutant HIV-1 genomic cDNA is inserted into a plasmid also treated with the restriction enzymes PflMI and EcoRI. Prepare.
- a plasmid containing a gene in which a mutation has been introduced into the start codon of the above vpr gene is treated with the restriction enzymes Ball and Spel, and a part of the gag-pol region (base number in SEQ ID NO: 2; A fragment prepared by blunt-ending the fragment from which 1511-4551) has been deleted and then performing self-ligation can be used.
- An expression vector containing a gene encoding the Vpr protein is a HIV-1 minute isolated NL432 infectious molecular clone (Adachi et al., J. Virology, 59 (2): 284-291, 1986).
- PBluescriptll-S which was obtained by amplifying the vpr gene of The type inserted into the EcoRV and Xbal sites of K (+) (FvprZpSK + II) is type III, and the type inserted into the Xhol and Notl sites of the expression plasmid pME-18Neo vector for mammalian cells (FvprZpME18Neo) ( Nishino et al., Biochem. Biophys. Res. Commun., 232 (2): 550-554, 1997).
- the present invention provides a method for screening a substance that suppresses the growth of HIV-1, comprising the following steps:
- the transfected cells are cultured in the presence of the candidate substance for 24 hours to 72 hours, preferably 48 hours to 72 hours (this time may be determined by a person skilled in the art depending on the cells used and the culture conditions). May be determined);
- a method comprising:
- the vector containing the HIV-1 genome that exhibits an expression pattern similar to that of the wild-type HIV-1 gene is a vector containing the HIV-1 cDNA NL432 (SEQ ID NO: 2; GenBank accession number M19921).
- the vector comprising the mutant HIV-1 genome is a part of the Gag-Pol region of the HIV-1 genome (SEQ ID NO: 2; GenBank accession number M19921) in the HIV-1 cDNA NL432 (SEQ ID NO: 2).
- This vector contains the mutated HIV-1 genomic cDNA lacking the nucleotide numbers 1511-4551) in No. 2.
- the present invention provides a method for screening a substance that suppresses the growth of HIV-1, comprising the following steps:
- a mutant HIV-1 genome which shows the same expression pattern as the wild-type HIV-1 gene and is mutated so as not to express the Vpr protein, and an expression cassette different from the mutant HIV-1 genome Transfecting a cell with a vector comprising a gene encoding an integrated Vpr protein;
- the transfected cells are cultured in the presence of the candidate substance for 24 hours to 72 hours, preferably 48 hours to 72 hours (this time may be determined by a person skilled in the art depending on the cells used and the culture conditions). May be determined);
- a method comprising:
- the cell into which the gene is to be introduced is not particularly limited as long as it is a mammalian cell, but is preferably a human-derived cell.
- human-derived cells are human-derived cultured cells, more preferably, HeLa cells, Jurkat cells, and the like.
- the cultivation depends on the cell system and can be performed by a method well known to those skilled in the art, but more detailed conditions may be determined by the skilled artisan as appropriate.
- Gene transfer can be carried out by a conventional method well known to those skilled in the art without particular limitation. It can be carried out by an extrusion method or an electoral-portation method.
- the culturing time after transfusion is 24 hours to 72 hours, and this time may be determined by a person skilled in the art according to the cell used and the culturing conditions. For example, at least 24 hours or more, desirably 48 hours, or 72 hours.
- RNA can be extracted from cells by a conventional method such as the AGPC method (acid guanidium-pheno-chloroform method) using a commercially available RNA extraction reagent such as Trizol reagent (Invitrogen).
- AGPC method acid guanidium-pheno-chloroform method
- Trizol reagent Invitrogen
- RT-PCR is carried out using primers that specifically amplify all types of HIV-1 mRNA and mRNAs of each form of 9 kb, 4 kb and 2 kb.
- RT-PCR can be performed, for example, using KOD + (TOYOBO) under the conditions of heating at 94 ° C for 2 minutes and appropriately repeating a cycle of 94 ° C for 60 seconds to 60 ° C for 60 seconds to 72 ° C for 60 seconds.
- the DNA fragment corresponding to the amplified mRNA of each form is quantified by, for example, a method using FLA-2000 (FUJIFILM).
- the quantification method may be a semi-quantitative method.
- the amount of this DNA fragment reflects the amount of each form of mRNA group.
- the amounts of DNA fragments corresponding to all types of HIV-1 mRNA are measured and compared with the experimental system and the control system, because the other 9 kb, 4 kb and 2 kb forms of the mRNA group This is for confirming whether or not the amount of generation is comparable.
- the amount of mRNA (all types) produced is the same in the experimental system and the control system, it is also possible to directly compare the difference in the amount of produced mRNA in each of the other forms.
- the ratio of the amount of production of the mRNA group of 4 kb form is 1.5
- the ratio of the production amount of all types of mRNA is 1. Since it is 0, it can be concluded that the increase in the amount of mRNA of the 4 kb form is caused by splicing control, not by the increase in the production of mRNA as a whole.
- the amount of the DNA fragment corresponding to the amplified mRNA group of each form of HIV-1 mRNA is determined by the presence of the candidate substance as compared to the control (p. ⁇ PSP ⁇ vpr and Vpr proteins are not expressed!
- the value close to the value derived from cells transfected with the control vector is 4 kb compared to the absence of the candidate substance due to the presence of the candidate substance.
- Form mRNA group A decrease in the amount of the corresponding DNA fragment, preferably a decrease in the amount of the DNA fragment corresponding to the 4 kb form of mRNA group and an increase in the amount of the DNA fragment corresponding to the 2 kb form of the mRNA group Say.
- the candidate substance inhibits the splicing inhibitory activity by the Vpr protein, it is determined that this substance is a substance that suppresses the proliferation of HIV-1.
- the candidate substance may be a biological substance such as a protein, a peptide, an antibody, or a nucleic acid, which may be a chemical substance.
- a biological substance such as a protein, a peptide, an antibody, or a nucleic acid
- the chemical substance a known compound may be used, or a conjugate library synthesized by a combinatorial chemistry technique known to those skilled in the art may be used.
- the biological material one having a sequence derived from nature may be used, or one having an artificial sequence may be used.
- a candidate substance to inhibit the splicing inhibitory activity of a Vpr protein can be evaluated in more detail by testing it in several dilution series.
- the mRNA group of the 2kb form and the 4kb form of HIV-1 contains more than 30 different mRNAs generated by alternative splicing (see Fig. 3).
- the present inventors have studied in detail the expression level of mRNA that constitutes each form of mRNA group, and found that in the presence of the Vpr protein, the 4 kb form of mRNA group was formed in the presence of the Vpr protein. It was found that among mRNAs, env mRNA increased, and the amount of tat mRNA among mRNAs constituting the 2 kb form mRNA group decreased (see FIGS. 4 and 5). This indicates that the Vpr protein controls the alternative splicing of HIV-1 mRNA by selectively inhibiting it, thereby increasing the expression of structural genes among the HIV-1 genes. Te ru.
- the screening method of the present invention comprises the following steps in place of the step (4):
- the amplified DNA fragments corresponding to the RNA groups of each form are subjected to gel electrophoresis (eg, acrylamide gel electrophoresis) to obtain DNA fragments corresponding to the mRNAs constituting the mRNA groups of each form. Separately detecting; and,
- the expression pattern of mRNA that constitutes the mRNA group of each form of HIV-1 that is determined by detecting the DNA fragment corresponding to each mRNA depends on the presence of the candidate substance. Control compared to the absence of quality (a vector containing the mutant HIV-1 genome described above, and no gene encoding the Vpr protein! /, Derived from cells transfected with the control vector) If the expression pattern approaches, the candidate substance is considered to be a substance that suppresses the growth of HIV-1;
- the presence of the candidate substance in the expression pattern of the mRNA constituting the mRNA group of each form of HIV-1, which is found by detecting the DNA fragment corresponding to each mRNA When the value of the control (from a cell into which a control vector that does not express p ⁇ PSP ⁇ vpr and Vpr protein is derived) is closer to the value of the control compared to the absence of the candidate substance, the presence of the candidate substance
- the value of the control from a cell into which a control vector that does not express p ⁇ PSP ⁇ vpr and Vpr protein is derived
- the presence of the candidate substance refers to a case where the amount of the DNA fragment corresponding to the env mRNA is reduced in the 4 kb form of the mRNA group compared to the absence of the substance, and desirably, the 4 kb form of the mRNA corresponds to the en V mRNA.
- this substance since the candidate substance inhibits splicing control by the Vpr protein, it is determined that this substance is a substance that suppresses the proliferation of HIV-1.
- the present invention provides primers that specifically amplify all types of HIV-1 mRNA, namely, 9 kb, 4 kb and 2 kb forms of mRNA groups.
- Each primer consists of a continuous 15-30 bases of DNA corresponding to the HIV-1 genome.
- a primer having the nucleotide sequence of SEQ ID NO: 6 was used as the forward primer, and the nucleotide sequence of SEQ ID NO: 7 was used as the reverse primer to amplify all types of HIV-1 mRNA.
- Primers having the nucleotide sequence of SEQ ID NO: 10 may be used as specific to the group (see FIG. 6).
- a primer pair represented by SEQ ID NO: 6 and SEQ ID NO: 7 To specifically amplify the mRNA group, a pair of primers represented by SEQ ID NO: 6 and SEQ ID NO: 9 to specifically amplify the mRNA group, a pair of primers represented by SEQ ID NO: 6 and 2 kb
- the primer pairs shown in SEQ ID NO: 6 and SEQ ID NO: 10 should be used, respectively.
- the primer of the present invention can be obtained by chemical synthesis using a phosphoramidite method known to those skilled in the art.
- the present invention relates to a pharmaceutical composition
- a pharmaceutical composition comprising, as an active ingredient, a substance that inhibits HIV-1 Vpr protein and thereby suppresses an increase in 4 kb form mRNA group and a decrease in 2 kb form mRNA group in HIV-1.
- the pharmaceutical composition of the present invention desirably contains a substance selected by the screening method of the present invention as an active ingredient.
- HIV-1 Preferably for controlling the growth of HIV-1.
- the pharmaceutical composition of the present invention may contain a pharmaceutically acceptable carrier or diluent.
- Pharmaceutically acceptable carriers or diluents and the like are essentially chemically inert and harmless compositions that have no effect on the biological activity of the pharmaceutical compositions of the present invention. Examples of such carriers or diluents include but are not limited to salts, sugar solutions, glycerol solutions, ethanol and the like.
- the pharmaceutical composition of the present invention is provided in a form that can be appropriately administered to a patient.
- the preparation form of the pharmaceutical composition of the present invention may be, for example, a liquid preparation such as an injection or a drip, or a freeze-dried preparation.
- the pharmaceutical composition of the present invention may contain an appropriate amount of any pharmaceutically acceptable additive, for example, an emulsifier, a stabilizer, an isotonic agent, and a pH adjuster.
- the lyophilized preparation can be prepared by subjecting the liquid preparation of the present invention to a lyophilization treatment.
- the freeze-drying treatment can be performed by a conventional method.
- the freeze-dried preparation of the present invention can be generally used by redissolving it with any suitable sterilized solution (redissolved solution) using a supplementary solution.
- a re-dissolved solution include an electrolyte solution such as water for injection and physiological saline, a glucose solution, and other general infusions.
- the pharmaceutical composition of the present invention can be administered intravenously, intraarterially, orally, intraosseously, transmucosally, or rectally to humans who want to administer in dosage unit form. And may be administered by any suitable method appropriate for the condition of the patient. It is administered in a dosage form suitable for these administration methods, for example, various injections, oral preparations, drops, suppositories and the like.
- the dose of the pharmaceutical composition of the present invention is desirably determined in consideration of the drug, the dosage form, the patient's condition such as age and weight, the administration route, the nature and extent of the disease, and the like.
- the physician will be able to determine the optimal dosage and dosage interval for an individual patient.
- the present inventors have determined that the HIV-1 Vpr protein is involved in the regulation of HIV-1 mRNA splicing and plays an important role in the replication-proliferation of HIV-1. Then, they reached the idea that the replication and proliferation of HIV-1 could be suppressed by suppressing the expression of the Vpr protein, and completed the present invention.
- siRNA short interference RNA
- siRNA is a double-stranded RNA having a sequence of 21 to 23 bases, and the 3 'end of each RNA has a protruding end of 2 to 3 bases.
- RISC protein-induced silencing complex
- the vpr mRNA is cleaved, and thus the expression of the Vpr protein is suppressed (knocked down).
- siRNA for the vpr gene By suppressing the expression of Vpr protein by siRNA for the vpr gene, the replication and growth of HIV-1 can be suppressed.
- the siRNA of the present invention includes a double-stranded RNA having a continuous sequence of 21 to 23 bases inside the vpr gene (SEQ ID NO: 5), and the 3 'end of each RNA has 2 to 3 bases. It is the protruding end of.
- the siRNA of the present invention may be prepared by chemical synthesis using a phosphoramidite method or the like.
- the present invention also relates to a pharmaceutical composition comprising the siRNA of the present invention.
- the pharmaceutical composition containing the siRNA of the present invention is used for suppressing the growth of HIV-1.
- the pharmaceutical composition comprising the siRNA of the present invention comprises a cationic carrier such as a cationic ribosome and a cationic polymer, or a window, which is effective for translocating the siRNA into cells. It may contain a carrier utilizing a lus envelope. Further, similarly to the pharmaceutical composition of the present invention, the pharmaceutical composition containing the siRNA of the present invention may contain a pharmaceutically acceptable carrier or diluent, and may be in a form that can be appropriately administered to a patient. Yes, dosage is determined by physician.
- a cationic carrier such as a cationic ribosome and a cationic polymer
- a window which is effective for translocating the siRNA into cells. It may contain a carrier utilizing a lus envelope.
- the pharmaceutical composition containing the siRNA of the present invention may contain a pharmaceutically acceptable carrier or diluent, and may be in a form that can be appropriately administered to a patient. Yes, dosage is determined by physician.
- the pharmaceutical composition containing the siRNA of the present invention also means a pharmaceutical composition containing a vector that expresses the siRNA of the present invention in a target cell instead of the siRNA of the present invention.
- a vector that expresses the siRNA of the present invention in a target cell instead of the siRNA of the present invention.
- the siRNA of the present invention is expressed in the cell, so that the same effects as those obtained by administering the siRNA of the present invention can be obtained.
- the present invention relates to an antisense nucleic acid for suppressing the expression of HIV-1 Vpr protein.
- the antisense nucleic acid of the present invention is composed of a complementary strand DNA having a continuous sequence of 15 to 30 bases inside the vpr gene (SEQ ID NO: 5).
- the antisense nucleic acid of the present invention may be prepared by chemical synthesis using the phosphoramidite method or the like.
- the present invention also relates to a pharmaceutical composition comprising the antisense nucleic acid of the present invention.
- the pharmaceutical composition containing the antisense nucleic acid of the present invention is used for suppressing the growth of HIV-1.
- the pharmaceutical composition containing the antisense nucleic acid of the present invention utilizes a cationic carrier such as a cationic ribosome and a cationic polymer, or a virus envelope, which is effective for transferring the antisense nucleic acid into cells. May be included.
- the pharmaceutical composition containing the antisense nucleic acid of the present invention may contain a pharmaceutically acceptable carrier or diluent, etc., as in the case of the pharmaceutical composition of the present invention. Form, the dose being determined by the physician.
- the present invention provides a novel anti-HIV-1 drug and a method for screening the same, thereby providing a new therapeutic method and a means for developing the same in the field of AIDS treatment in which the emergence of drug-resistant viruses has become a problem.
- the screening method and the pharmaceutical composition of the present invention target a completely novel mechanism in the replication and amplification processes of a virus after infection, which has been conventionally targeted for the treatment, and are widely used in the treatment of AIDS. Giving it a gender can make a difference.
- FIG. 1 is a schematic diagram of expression patterns of mRNA groups of each form of HIV-1 during the early stage and the late stage of HIV-1 infection.
- FIG. 2 is a graph showing the ratio of the amount of the mRNA group of each form at 48 hours after transfection of HeLa cells with pAPSPAvpr and Vpr protein expression vectors, and pAPSPA vpr and control vectors. It is.
- LTR indicates total mRNA
- 9K indicates a 9 kb form mRNA group
- 4K indicates a 4 kb form mRNA group
- 2K indicates a 2 kb form mRNA group.
- FIG. 3 is a view showing mRNAs constituting mRNA groups of 2 kb form and 4 kb form of HIV-1.
- FIG. 4 is a photograph showing the results of analysis of a 4 kb form mRNA group by gel electrophoresis, and the results of the analysis of the mRNA constituting the 4 kb form mRNA group in the absence of Vpr protein versus the presence of Vpr protein. It is a graph which shows the ratio of the amount of production.
- lane 1 was not transfection
- lane 2 was transfection with pAPSPAvpr and Vpr protein expression vector
- lane 3 was transfection with p ⁇ SPA vpr and control vector. .
- FIG. 5 is a photograph showing the results of gel electrophoresis analysis of the 2 kb form mRNA group, and the results of the analysis of the mRNA constituting the 2 kb form mRNA group in the absence of Vpr protein and in the presence of Vpr protein. It is a graph which shows the ratio of the amount of production.
- lane 1 was not transfection
- lane 2 was transfection with pAPSPAvpr and Vpr protein expression vector
- lane 3 was transfection with p ⁇ SPA vpr and control vector. .
- FIG. 6 shows primers for amplifying total mRNA, 9 kb form mRNA group, 4 kb form mRNA group, and 2 kb form mRNA group, which are generated from pA PSP Avpr. It is a figure showing a position.
- Example 1 Effect of HIV-1 Vpr protein on splicing of viral genome
- HIV-1 cDNA NL432 (SEQ ID NO: 2: GenBank Accession No. M19921) was treated with restriction enzymes Ndel and sail, and the excised fragment was blunt-ended and incorporated into pBluescript SK +. .
- the ATG was mutated from GTG to the start codon of the vpr gene (base numbers 5559-5561 in SEQ ID NO: 2) in this plasmid.
- a fragment was excised from the mutated plasmid with the restriction enzymes PflMI and EcoRI, and then incorporated into N432 treated with the restriction enzymes PflMI and EcoRI in the same manner to prepare NL432 Avpr.
- NL432 ⁇ vpr is treated with the restriction enzymes Ball and Spel, and a fragment from which a part of the gag-pol region (base numbers 1511-4551 in SEQ ID NO: 2) has been deleted is blunt-ended, followed by self-ligation.
- the plasmid ⁇ PSP ⁇ vpr containing the mutant HIV-1 genomic cDNA (SEQ ID NO: 4) was used.
- An expression vector containing the gene encoding the Vpr protein (SEQ ID NO: 5) is an HIV-1 minute isolated NL432 infectious molecular clone (Adachi et al., J. Virology, 59 (2): 284-291, 1986).
- control vector As the control vector, the above-described Vpr expression vector in which the sequence CAA encoding the third amino acid of the Vpr protein was mutated to a termination codon TAA was used. Therefore, Vpr protein is not expressed from the control vector!
- the pAPSP Avpr described above was introduced into HeLa cells together with a Vpr expression vector or control vector by the transfusion method. Specifically, transfection was performed using Lipofectamine Plus (Invitrogen) according to the manufacturer's protocol. The transfected cells were cultured at 37 ° C in the presence of 5% CO. 24 hours after starting culture
- Primers were designed and synthesized to specifically increase the mRNA group of each of the 9 kb, 4 kb, and 2 kb forms of HIV-1 mRNA. Specifically, the od p045 primer (SEQ ID NO: 6) was used as the forward primer, and the 744 (Total) primer (SEQ ID NO: 7) and 9Kb were used as reverse primers to amplify all types of HIV-1 mRNA.
- Gag primer (SEQ ID NO: 8) specific to the form mRNA group, KPNA primer (SEQ ID NO: 9) specific to the 4Kb form mRNA group, and SJ4.7A primer (SEQ ID NO: 9) specific to the 2Kb form mRNA group 10) was used (see Figure 6).
- the RNA from which the above-mentioned cellular power was also extracted was type III, and DNA fragments corresponding to mRNA groups of each form were semi-quantitatively amplified by semi-quantitative RT PCR. Since the amount of this DNA fragment reflects the amount of the mRNA group of each form, by observing the amount of the amplified DNA fragment, It was observed whether the amount of foam mRNA group changed.
- RNA extracted 24 hours after the transfection no change in the amount of the mRNA group of each form of HIV-1 based on the presence or absence of the Vpr protein was observed.
- the RNA extracted at 48 hours when the Vpr protein was present, an increase in the 4 kb form mRNA group and a decrease in the 2 kb form mRNA group were observed (see FIG. 2).
- HIV-1 genomic RNA is in a two-spliced 2kb (1.8 kb) form that translocates out of the nucleus and is regulated by this RNA in the cytoplasm.
- the genes Rev, Tat, and Nef are expressed.
- the 9 kb form, a fully genomic RNA, and the once-spliced 4 kb form cannot translocate to the nucleus and remain in the nucleus.
- Rev binds to the RRE (Rev response element) sequence in the pre-mRNA, transports 4 kb and 9 kb forms of RNA to the nucleus, and Env, Gag , Pol, and other proteins that make up the virus itself (see Figure 1: Annu. Rev. Microbiol., 52: 491-532, 1998).
- RRE Rev response element
- Vpr protein has a function of promoting the generation of a 4 kb form mRNA group while suppressing the generation of a 2 kb form RNA group. This suggests that inhibition of splicing of the viral genome by the Vpr protein leads to the HIV-1 virus infection stage in the process of virus replication and propagation!
- the 2 kb and 4 kb forms of HIV-1 mRNAs contain more than 30 different mRNAs that result from alternative splicing (see Figure 3).
- This pattern of alternative splicing clearly demonstrates the regulation of expression of individual genes, such as Envs, regulatory genes, and accessory genes (J. Virology, 6365-6378, 1993). ).
- the effect of the Vpr protein on alternative splicing was observed by examining the mRNA species contained in each form of the mRNA group in the presence and absence of the Vpr protein.
- the semi-quantitative RT-PCR product obtained in the same manner as in Example 1 was swim on a 4% acrylamide gel, stained with SYBR Green (Molecular Probe Inc.), and analyzed with a fluorescence image analyzer (FLA- 2000: FUJIFILM).
- Example 3 Influence of the splicing pattern of the viral genome in the presence of HTV-II Vnr protein in HTV-1 sensitive cells
- Molt4 cells were infected with HIV-1NL432 (SEQ ID NO: 2: GenBank Accession No. M19921) or HIV-1 NL432 Avpr to prepare HIV-1 infected cells. After culturing HIV-1 infected cells for 2, 3, 4 or 8 days, total RNA is extracted from the cells and the primers are specific for each of LTR, 9kb form, env, tat, rev, nef, and vpr. Real-time PCR (Roche) quantified according to the manufacturer's protocol.
- Example 2 ie, p ⁇ PSP ⁇ vpr and Vpr expression vector, or ⁇ PSP
- the expression pattern of HIV-1 mRNA when the ⁇ vpr and control vector were introduced into HeLa cells was measured by acrylamide gel electrophoresis (Figs. 4 and 5). Also, in the presence of Vpr, an increase in env mRNA and rev mRNA and a decrease in tat mRNA were observed on day 8 after infection (FIG. 7). A similar tendency was observed in RNA extracted on days 2, 3, and 4 after infection. This suggests that Vpr protein affects the mRNA patterns of env, rev, and tat even in HIV-1-infected cells.
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US20130203837A1 (en) * | 2010-05-26 | 2013-08-08 | Chenyu Zhang | PREPARATION OF MICROVESICLE-siRNA COMPLEXES AND USE THEREOF IN AIDS TREATMENT |
US9376679B2 (en) | 2010-05-26 | 2016-06-28 | Micromedmark Biotech Co. Ltd. | Microvesicles carrying small interfering RNAs, preparation methods and uses thereof |
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JACQUENET, S. ET AL.: "Conserved stem-loop structures in the HIV-1 RNA region containing the A3 3' splice site and its cis-regulatory element: possible involvement in RNA splicing", NUCLEIC ACIDS RESEARCH, vol. 29, 2001, pages 464 - 478, XP002983149 * |
NISHIZAWA, M. ET AL.: "A Carboxy-Terminally Truncated Form of Vpr Protein of Human Immunodeficiency Virus Type 1 Retards Cell Proliferation", VIROLOGY, vol. 263, 1999, pages 313 - 322, XP004439749 * |
NISHIZAWA, M. ET AL.: "Induction of Apoptosis by the Vpr Protein of Human Immunodeficiency Virus Type 1 Occurs Independently of G2 Arrest of the Cell Cycle", VIROLOGY, vol. 276, 2000, pages 16 - 26, XP004436203 * |
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US20130203837A1 (en) * | 2010-05-26 | 2013-08-08 | Chenyu Zhang | PREPARATION OF MICROVESICLE-siRNA COMPLEXES AND USE THEREOF IN AIDS TREATMENT |
US9376679B2 (en) | 2010-05-26 | 2016-06-28 | Micromedmark Biotech Co. Ltd. | Microvesicles carrying small interfering RNAs, preparation methods and uses thereof |
US9421167B2 (en) * | 2010-05-26 | 2016-08-23 | Micromedmark Biotech Co. Ltd. | Preparation of microvesicle-siRNA complexes and use thereof in AIDS treatment |
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