HRP20020186A2 - Tenascin-c nucleic acid ligands - Google Patents
Tenascin-c nucleic acid ligands Download PDFInfo
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- HRP20020186A2 HRP20020186A2 HR20020186A HRP20020186A HRP20020186A2 HR P20020186 A2 HRP20020186 A2 HR P20020186A2 HR 20020186 A HR20020186 A HR 20020186A HR P20020186 A HRP20020186 A HR P20020186A HR P20020186 A2 HRP20020186 A2 HR P20020186A2
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- Prior art keywords
- tenascin
- nucleic acid
- nucleic acids
- mixture
- complex
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Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/364,902 US6232071B1 (en) | 1990-06-11 | 1999-07-29 | Tenascin-C nucleic acid ligands |
PCT/US2000/002167 WO2001009390A1 (en) | 1999-07-29 | 2000-01-28 | Tenascin-c nucleic acid ligands |
Publications (1)
Publication Number | Publication Date |
---|---|
HRP20020186A2 true HRP20020186A2 (en) | 2004-02-29 |
Family
ID=23436596
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HR20020186A HRP20020186A2 (en) | 1999-07-29 | 2002-02-28 | Tenascin-c nucleic acid ligands |
Country Status (29)
Country | Link |
---|---|
US (4) | US6232071B1 (no) |
EP (1) | EP1198589B9 (no) |
JP (1) | JP2003505111A (no) |
KR (1) | KR100605072B1 (no) |
CN (2) | CN100558411C (no) |
AT (1) | ATE405675T1 (no) |
AU (1) | AU767501C (no) |
BG (1) | BG106361A (no) |
BR (1) | BR0013170A (no) |
CA (1) | CA2380473A1 (no) |
CZ (1) | CZ2002365A3 (no) |
DE (1) | DE60039986D1 (no) |
DK (1) | DK1198589T3 (no) |
EA (1) | EA004795B1 (no) |
EE (1) | EE200200051A (no) |
ES (1) | ES2310989T3 (no) |
HK (1) | HK1048499B (no) |
HR (1) | HRP20020186A2 (no) |
HU (1) | HUP0202221A3 (no) |
IL (2) | IL147872A0 (no) |
MX (1) | MXPA02000819A (no) |
NO (1) | NO20020424L (no) |
NZ (2) | NZ516907A (no) |
PL (1) | PL201637B1 (no) |
RS (1) | RS50426B (no) |
SK (1) | SK1222002A3 (no) |
UA (1) | UA75578C2 (no) |
WO (1) | WO2001009390A1 (no) |
ZA (1) | ZA200200747B (no) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6232071B1 (en) * | 1990-06-11 | 2001-05-15 | Gilead Sciences, Inc. | Tenascin-C nucleic acid ligands |
US7005260B1 (en) * | 2000-01-28 | 2006-02-28 | Gilead Sciences, Inc. | Tenascin-C nucleic acid ligands |
CA2328356A1 (en) * | 1999-12-22 | 2001-06-22 | Itty Atcravi | Recreational vehicles |
US7645743B2 (en) * | 1999-12-22 | 2010-01-12 | Altermune, Llc | Chemically programmable immunity |
US7300922B2 (en) | 2001-05-25 | 2007-11-27 | Duke University | Modulators of pharmacological agents |
EP1552002A4 (en) * | 2002-06-18 | 2006-02-08 | Archemix Corp | APTAMER TOXIN MOLECULES AND METHOD FOR THEIR USE |
US20040249130A1 (en) * | 2002-06-18 | 2004-12-09 | Martin Stanton | Aptamer-toxin molecules and methods for using same |
US8039443B2 (en) * | 2002-11-21 | 2011-10-18 | Archemix Corporation | Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics |
US8853376B2 (en) | 2002-11-21 | 2014-10-07 | Archemix Llc | Stabilized aptamers to platelet derived growth factor and their use as oncology therapeutics |
US10100316B2 (en) | 2002-11-21 | 2018-10-16 | Archemix Llc | Aptamers comprising CPG motifs |
US7727969B2 (en) * | 2003-06-06 | 2010-06-01 | Massachusetts Institute Of Technology | Controlled release nanoparticle having bound oligonucleotide for targeted delivery |
WO2006093932A2 (en) * | 2005-03-01 | 2006-09-08 | Cedars-Sinai Medical Center | Use of eotaxin as a diagnostic indicator for atherosclerosis and vascular inflammation |
AR052741A1 (es) * | 2005-04-08 | 2007-03-28 | Noxxon Pharma Ag | Acidos nucleicos de union a ghrelin |
EP1897562A1 (en) * | 2006-09-08 | 2008-03-12 | Bayer Schering Pharma Aktiengesellschaft | Aptamers labelled with Gallium-68 |
WO2008028688A2 (en) | 2006-09-08 | 2008-03-13 | Bayer Schering Pharma Aktiengesellschaft | Compounds and methods for 18f labeled agents |
US20110136099A1 (en) | 2007-01-16 | 2011-06-09 | Somalogic, Inc. | Multiplexed Analyses of Test Samples |
US8975026B2 (en) | 2007-01-16 | 2015-03-10 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
EP2172566B2 (en) * | 2007-07-17 | 2022-05-18 | Somalogic, Inc. | Method for generating aptamers with improved off-rates |
EP2036981A1 (en) * | 2007-09-12 | 2009-03-18 | Bayer Schering Pharma Aktiengesellschaft | Aptamers labeled with 18F |
CA2760774A1 (en) | 2009-05-05 | 2010-11-11 | Altermune Technologies, Llc | Chemically programmable immunity |
US8236570B2 (en) | 2009-11-03 | 2012-08-07 | Infoscitex | Methods for identifying nucleic acid ligands |
US8841429B2 (en) | 2009-11-03 | 2014-09-23 | Vivonics, Inc. | Nucleic acid ligands against infectious prions |
GB201114662D0 (en) | 2011-08-24 | 2011-10-12 | Altermune Technologies Llc | Chemically programmable immunity |
CN102533772B (zh) * | 2011-12-12 | 2013-10-09 | 中国科学院武汉病毒研究所 | 一种抗hbv诱饵适体及其筛选方法 |
KR20140109956A (ko) * | 2011-12-12 | 2014-09-16 | 아이시스 이노베이션 리미티드 | 테나신-c 및 류마티스 관절염에서의 이의 용도 |
KR20220139425A (ko) | 2012-07-13 | 2022-10-14 | 웨이브 라이프 사이언시스 리미티드 | 키랄 제어 |
CN107058596A (zh) * | 2017-06-19 | 2017-08-18 | 上海市第十人民医院 | 一种与恶性胶质瘤诊断相关的标志物及其应用 |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1186660A3 (fr) | 1985-03-30 | 2002-03-20 | KAUFFMAN, Stuart A. | Procédé d'obtension d'ADN, ARN, peptides, polypeptides ou protéines, par une technique de recombination d'ADN |
WO1989006694A1 (en) | 1988-01-15 | 1989-07-27 | Trustees Of The University Of Pennsylvania | Process for selection of proteinaceous substances which mimic growth-inducing molecules |
US5789157A (en) * | 1990-06-11 | 1998-08-04 | Nexstar Pharmaceuticals, Inc. | Systematic evolution of ligands by exponential enrichment: tissue selex |
US6610841B1 (en) * | 1997-12-18 | 2003-08-26 | Gilead Sciences, Inc. | Nucleotide-based prodrugs |
US5496938A (en) * | 1990-06-11 | 1996-03-05 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands to HIV-RT and HIV-1 rev |
US6232071B1 (en) * | 1990-06-11 | 2001-05-15 | Gilead Sciences, Inc. | Tenascin-C nucleic acid ligands |
US6127119A (en) * | 1990-06-11 | 2000-10-03 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands of tissue target |
KR970002255B1 (ko) | 1990-06-11 | 1997-02-26 | 넥스스타 파아마슈티컬드, 인크. | 핵산 리간드 |
IE920562A1 (en) | 1991-02-21 | 1992-08-26 | Gilead Sciences | Aptamer specific for biomolecules and method of making |
US5683874A (en) * | 1991-03-27 | 1997-11-04 | Research Corporation Technologies, Inc. | Single-stranded circular oligonucleotides capable of forming a triplex with a target sequence |
US5582981A (en) * | 1991-08-14 | 1996-12-10 | Gilead Sciences, Inc. | Method for identifying an oligonucleotide aptamer specific for a target |
US5436132A (en) * | 1993-02-13 | 1995-07-25 | Amano Pharmaceutical Co., Ltd. | Quantitative determination of tenascin as glioma marker |
US5859228A (en) * | 1995-05-04 | 1999-01-12 | Nexstar Pharmaceuticals, Inc. | Vascular endothelial growth factor (VEGF) nucleic acid ligand complexes |
US6229002B1 (en) * | 1995-06-07 | 2001-05-08 | Nexstar Pharmaceuticlas, Inc. | Platelet derived growth factor (PDGF) nucleic acid ligand complexes |
PT957929E (pt) * | 1996-10-25 | 2006-06-30 | Gilead Sciences Inc | Complexos de ligandos de acido nucleico do factor de crescimento endotelial vascular |
US6242246B1 (en) * | 1997-12-15 | 2001-06-05 | Somalogic, Inc. | Nucleic acid ligand diagnostic Biochip |
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1999
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2000
- 2000-01-28 NZ NZ516907A patent/NZ516907A/en unknown
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- 2000-01-28 DK DK00911657T patent/DK1198589T3/da active
- 2000-01-28 AT AT00911657T patent/ATE405675T1/de not_active IP Right Cessation
- 2000-01-28 ES ES00911657T patent/ES2310989T3/es not_active Expired - Lifetime
- 2000-01-28 AU AU33519/00A patent/AU767501C/en not_active Ceased
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- 2000-01-28 CA CA002380473A patent/CA2380473A1/en not_active Abandoned
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2001
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2002
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2003
- 2003-01-24 HK HK03100657.1A patent/HK1048499B/zh not_active IP Right Cessation
- 2003-05-01 US US10/429,176 patent/US20040058884A1/en not_active Abandoned
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