HK1221710A1 - 種基於分子反向探針的測序文庫構建方法和試劑 - Google Patents

種基於分子反向探針的測序文庫構建方法和試劑

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Publication number
HK1221710A1
HK1221710A1 HK16109811.0A HK16109811A HK1221710A1 HK 1221710 A1 HK1221710 A1 HK 1221710A1 HK 16109811 A HK16109811 A HK 16109811A HK 1221710 A1 HK1221710 A1 HK 1221710A1
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HK
Hong Kong
Prior art keywords
reagent
sequencing library
library based
molecular inversion
inversion probe
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HK16109811.0A
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English (en)
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Publication of HK1221710A1 publication Critical patent/HK1221710A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK16109811.0A 2014-12-22 2016-08-16 種基於分子反向探針的測序文庫構建方法和試劑 HK1221710A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410808647 2014-12-22

Publications (1)

Publication Number Publication Date
HK1221710A1 true HK1221710A1 (zh) 2017-06-09

Family

ID=56146968

Family Applications (1)

Application Number Title Priority Date Filing Date
HK16109811.0A HK1221710A1 (zh) 2014-12-22 2016-08-16 種基於分子反向探針的測序文庫構建方法和試劑

Country Status (2)

Country Link
CN (1) CN105714383B (zh)
HK (1) HK1221710A1 (zh)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106399519B (zh) * 2016-09-30 2019-07-26 南京迪康金诺生物技术有限公司 一种基于发卡结构的目标区域捕获方法及其应用
WO2018214036A1 (zh) * 2017-05-23 2018-11-29 深圳华大基因股份有限公司 基于滚环扩增的基因组目标区域富集方法及其应用
CN110021353B (zh) * 2017-09-30 2020-11-06 厦门艾德生物医药科技股份有限公司 一种用于捕获富集基因组的特定区域的分子反向探针的筛选方法
CN109593757B (zh) * 2017-09-30 2021-08-03 厦门艾德生物医药科技股份有限公司 一种探针及其适用于高通量测序的对目标区域进行富集的方法
CN109694908B (zh) * 2017-10-24 2023-01-10 深圳乐土生物科技有限公司 检测耳聋基因20个突变位点的快速文库构建方法及检测方法
CN107586847B (zh) * 2017-10-30 2024-06-21 北京钟楣科技有限公司 一种环形接头及其应用
CN110366598B (zh) * 2017-12-29 2022-05-10 行动基因生技股份有限公司 序列比对与突变位点分析的方法及系统
CN110079592B (zh) * 2018-01-26 2021-02-12 厦门艾德生物医药科技股份有限公司 用于检测基因突变和已知、未知基因融合类型的高通量测序靶向捕获目标区域的探针和方法
CN108396057B (zh) * 2018-02-28 2021-11-09 重庆市肿瘤研究所 基于长链分子倒置探针的核酸靶向捕获测序文库制备方法
CN108486101B (zh) * 2018-04-03 2021-01-08 艾吉泰康生物科技(北京)有限公司 一种快速捕获建库的方法
CN108504722A (zh) * 2018-04-09 2018-09-07 殷东敏 一种基于锁型探针等温扩增快速检测串联重复序列的方法
CN108660194A (zh) * 2018-06-26 2018-10-16 中南大学湘雅医院 一种帕金森病致病基因突变筛查检测方法
CN109576346B (zh) * 2018-11-05 2022-06-10 深圳市艾斯基因科技有限公司 高通量测序文库的构建方法及其应用
CN111349718A (zh) * 2018-12-21 2020-06-30 深圳华大智造科技有限公司 用于病原核酸扩增的引物组、病原核酸检测文库构建方法和病原检测方法
WO2020191521A1 (zh) * 2019-03-22 2020-10-01 深圳华大智造科技有限公司 核酸序列、rna目标区域测序文库的构建方法及应用
CN110241177A (zh) * 2019-04-19 2019-09-17 上海三誉华夏基因科技有限公司 基于杂交、延伸连接反应的核酸捕获文库制备方法
CN113122612B (zh) * 2019-12-31 2023-06-27 深圳华大智造科技股份有限公司 一种用于目标区域捕获测序的dna捕获探针制备方法及其应用
CN112063690A (zh) * 2020-09-18 2020-12-11 北京求臻医学检验实验室有限公司 单分子探针多重靶向捕获文库的构建方法及应用
CN112322759A (zh) * 2020-12-10 2021-02-05 镇江华大检测有限公司 一种基于高通量测序鉴定三种鳕鱼的检测方法
CN113658638B (zh) * 2021-08-20 2022-06-03 江苏先声医学诊断有限公司 一种基于ngs平台的同源重组缺陷的检测方法和质控体系
CN114836415B (zh) * 2022-05-06 2023-04-18 南京诺唯赞生物科技股份有限公司 一种mgi平台转座酶双端标签文库的制备
WO2023240611A1 (zh) * 2022-06-17 2023-12-21 深圳华大智造科技股份有限公司 单链核酸环状文库的建库以及测序方法
CN115873927B (zh) * 2022-08-04 2023-12-26 迈杰转化医学研究(苏州)有限公司 一种不依赖连接的核酸恒温滚环扩增方法及其应用
CN115873928B (zh) * 2022-09-02 2024-01-09 迈杰转化医学研究(苏州)有限公司 一种基于双链环模板的滚环扩增方法及其应用
CN116064758A (zh) * 2022-11-17 2023-05-05 纳昂达(南京)生物科技有限公司 选择抑制性环化辅助序列及应用
CN116497088A (zh) * 2023-04-26 2023-07-28 苏州吉因加生物医学工程有限公司 一种夹板核酸分子及其试剂盒

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4372084A3 (en) * 2012-01-26 2024-08-14 Tecan Genomics, Inc. Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
CN103642944B (zh) * 2013-12-17 2016-08-17 中国人民解放军第三军医大学第三附属医院 用于检测乙型肝炎病毒yvdd耐药突变的分子倒置探针及其应用

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CN105714383B (zh) 2018-01-23

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