HK1198445A1 - On-column enzymatic cleavage - Google Patents

On-column enzymatic cleavage

Info

Publication number
HK1198445A1
HK1198445A1 HK14111935A HK14111935A HK1198445A1 HK 1198445 A1 HK1198445 A1 HK 1198445A1 HK 14111935 A HK14111935 A HK 14111935A HK 14111935 A HK14111935 A HK 14111935A HK 1198445 A1 HK1198445 A1 HK 1198445A1
Authority
HK
Hong Kong
Prior art keywords
enzymatic cleavage
column
column enzymatic
cleavage
enzymatic
Prior art date
Application number
HK14111935A
Other languages
English (en)
Chinese (zh)
Inventor
羅伯托．法爾肯施泰因
阿德爾伯特．格羅斯曼
弗里德里克．黑塞
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of HK1198445A1 publication Critical patent/HK1198445A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/775—Apolipopeptides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4726—Lectins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
HK14111935A 2012-02-29 2014-11-26 On-column enzymatic cleavage HK1198445A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12157510 2012-02-29
PCT/EP2013/053872 WO2013127816A1 (en) 2012-02-29 2013-02-27 On-column enzymatic cleavage

Publications (1)

Publication Number Publication Date
HK1198445A1 true HK1198445A1 (en) 2015-04-24

Family

ID=47750700

Family Applications (1)

Application Number Title Priority Date Filing Date
HK14111935A HK1198445A1 (en) 2012-02-29 2014-11-26 On-column enzymatic cleavage

Country Status (10)

Country Link
US (1) US20150044718A1 (ja)
EP (1) EP2820031A1 (ja)
JP (1) JP2015508664A (ja)
KR (1) KR20140135959A (ja)
CN (1) CN104144939A (ja)
CA (1) CA2862820A1 (ja)
HK (1) HK1198445A1 (ja)
MX (1) MX2014010092A (ja)
RU (1) RU2014137623A (ja)
WO (1) WO2013127816A1 (ja)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6207507B2 (ja) 2011-08-25 2017-10-04 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 短縮されたテトラネクチン−アポリポプロテインａ−ｉ融合タンパク質、それを含む脂質粒子、及びその使用
CA2929149A1 (en) * 2013-12-20 2015-06-25 F. Hoffmann-La Roche Ag Improved recombinant polypeptide production methods
BR112017024610A2 (pt) 2015-06-24 2018-07-31 F. Hoffmann-La Roche Ag anticorpos para receptor antitransferrina com afinidade especificada
AR106189A1 (es) 2015-10-02 2017-12-20 Hoffmann La Roche ANTICUERPOS BIESPECÍFICOS CONTRA EL A-b HUMANO Y EL RECEPTOR DE TRANSFERRINA HUMANO Y MÉTODOS DE USO
CN114031689A (zh) 2015-10-02 2022-02-11 豪夫迈·罗氏有限公司 双特异性抗人cd20/人转铁蛋白受体抗体及使用方法
US11360079B2 (en) 2015-12-16 2022-06-14 Neogap Therapeutics Ab T-cell reactivity platform
MX2019008996A (es) * 2017-01-30 2019-09-11 Regeneron Pharma Composiciones y metodos para reducir la carga biologica en cromatografia.
CN114790473A (zh) * 2021-11-08 2022-07-26 汉肽生物医药集团有限公司 一种利拉鲁肽融合蛋白在位酶切和纯化的方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0422237A4 (en) 1989-03-16 1992-01-02 Nippon Kayaku Kabushiki Kaisha Liquid composition and method of dyeing therewith
SE500941C2 (sv) 1989-08-16 1994-10-03 Algy Persson Förfarande och apparat för snittning av ett preparat
BR9709569B1 (pt) * 1996-06-11 2010-05-18 processo para ativação de proteìna desnaturada.
EP0972838B1 (en) 1998-07-15 2004-09-15 Roche Diagnostics GmbH Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy
US6291245B1 (en) 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
US6514741B1 (en) * 1999-08-18 2003-02-04 Zymogenetics, Inc. Tryptase-like polypeptide ztryp1
US6969757B2 (en) * 2001-01-26 2005-11-29 Syngenta Participations Ag Differential labeling for quantitative analysis of complex protein mixtures
AU2005265163B2 (en) * 2004-06-18 2009-10-01 Ambrx, Inc. Novel antigen-binding polypeptides and their uses
US7893197B2 (en) * 2004-08-25 2011-02-22 Janssen Pharmaceutica N.V. Relaxin-3 chimeric polypeptides and their preparation and use
JP4958975B2 (ja) 2006-08-31 2012-06-20 エフ．ホフマン−ラ ロシュ アーゲー インスリン様増殖因子ｉの製造方法
WO2009034190A2 (en) * 2007-09-14 2009-03-19 Genimmune N.V. Affinity tag
US20100297734A1 (en) * 2007-10-12 2010-11-25 University Health Network Fusion Tag Comprising an Affinity Tag and an EF-Hand Motif Containing Polypeptide and Methods of Use Thereof
US8034910B2 (en) * 2008-05-06 2011-10-11 Academia Sinica, Taiwan SUMO fusion protein expression system for producing native proteins
CN103068368A (zh) 2010-08-30 2013-04-24 霍夫曼-拉罗奇有限公司 产生四连蛋白-载脂蛋白a-i脂质颗粒的方法，脂质颗粒本身及其应用
WO2012028526A2 (en) 2010-08-30 2012-03-08 F. Hoffmann-La Roche Ag Tetranectin-apolipoprotein a-i, lipid particles containing it and its use

Also Published As

Publication number Publication date
WO2013127816A1 (en) 2013-09-06
RU2014137623A (ru) 2016-04-20
US20150044718A1 (en) 2015-02-12
JP2015508664A (ja) 2015-03-23
KR20140135959A (ko) 2014-11-27
CA2862820A1 (en) 2013-09-06
EP2820031A1 (en) 2015-01-07
CN104144939A (zh) 2014-11-12
MX2014010092A (es) 2014-09-16

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