HK1142928A1 - 在經修飾的隨機寡核苷酸的存在下的核酸擴增 - Google Patents

在經修飾的隨機寡核苷酸的存在下的核酸擴增

Info

Publication number
HK1142928A1
HK1142928A1 HK10109219.4A HK10109219A HK1142928A1 HK 1142928 A1 HK1142928 A1 HK 1142928A1 HK 10109219 A HK10109219 A HK 10109219A HK 1142928 A1 HK1142928 A1 HK 1142928A1
Authority
HK
Hong Kong
Prior art keywords
randomers
modified
nucleic acid
acid amplification
amplification
Prior art date
Application number
HK10109219.4A
Other languages
English (en)
Inventor
Ankenbauer Waltraud
Heindl Dieter
Laue Frank
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1142928A1 publication Critical patent/HK1142928A1/zh

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
HK10109219.4A 2008-03-19 2010-09-27 在經修飾的隨機寡核苷酸的存在下的核酸擴增 HK1142928A1 (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP08005100 2008-03-19

Publications (1)

Publication Number Publication Date
HK1142928A1 true HK1142928A1 (zh) 2010-12-17

Family

ID=39683448

Family Applications (1)

Application Number Title Priority Date Filing Date
HK10109219.4A HK1142928A1 (zh) 2008-03-19 2010-09-27 在經修飾的隨機寡核苷酸的存在下的核酸擴增

Country Status (7)

Country Link
US (2) US20090269766A1 (zh)
EP (1) EP2103693B1 (zh)
JP (1) JP5457055B2 (zh)
CN (1) CN101691593B (zh)
CA (1) CA2658520C (zh)
ES (1) ES2450073T3 (zh)
HK (1) HK1142928A1 (zh)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3150723B1 (en) 2010-06-21 2019-01-30 Life Technologies Corporation Compositions, kits, and methods for synthesis and/or detection of nucleic acids
DK3109326T3 (da) 2010-06-21 2022-02-14 Life Technologies Corp Sammensætning og fremgangsmåde til nukleinsyresyntese og amplifikation under anvendelse af rt
WO2013177429A2 (en) 2012-05-24 2013-11-28 University Of Utah Research Foundation Extreme pcr
PL230602B1 (pl) 2013-04-04 2018-11-30 Centrum Badan Molekularnych I Makromolekularnych Polskiej Akademii Nauk Kwas (E)-3-arylo-3-oksoprop-1-enylo-2-fosfonowy i jego pochodne, sposob ich wytwarzania oraz ich zastosowanie
PL223918B1 (pl) 2013-05-28 2016-11-30 Centrum Badań Molekularnych i Makromolekularnych Polskiej Akademii Nauk 3-Arylo-2-fosforylo podstawione 1-indanony i sposób ich wytwarzania
DK3337615T3 (da) 2015-11-05 2022-05-09 Univ Utah Res Found Ekstrem pcr via revers transkription
WO2018025899A1 (ja) * 2016-08-03 2018-02-08 和光純薬工業株式会社 プラスチック製マイクロ流体デバイス充填用溶液
JP2019198259A (ja) * 2018-05-15 2019-11-21 株式会社島津製作所 １ステップｒｔ−ｐｃｒ法
GB201812192D0 (en) 2018-07-26 2018-09-12 Ttp Plc Variable temperature reactor, heater and control circuit for the same

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5449603A (en) 1989-10-24 1995-09-12 Stratagene Method for hybridizing nucleic acids using single-stranded nucleic acid binding protein
CA2035010C (en) 1990-01-26 1996-12-10 Keith C. Backman Method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
DE69131891T2 (de) 1990-02-16 2000-06-15 Hoffmann La Roche Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
US5693502A (en) 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE4110808A1 (de) 1991-04-04 1992-10-08 Basf Ag Indolizintrimethinfarbstoffe
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US6033851A (en) 1994-12-09 2000-03-07 Wakunaga Seiyaku Kabushiki Kaisha Method for suppressing nonspecific hybridization in primer extension method
CA2168712A1 (en) 1995-02-07 1996-08-08 John William Henderson Sutherland Use of exonuclease and/or glycosylase as supplements to anti-polymerase antibody to increase specificity in polymerase chain reaction
CA2176193A1 (en) 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
WO1996041010A1 (en) 1995-06-07 1996-12-19 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands that bind to and inhibit dna polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
CA2257152C (en) 1996-06-03 2009-01-27 Linda M. Pilarski Methods for detection of rearranged dna
DE69735563T2 (de) 1996-06-04 2007-05-16 University Of Utah Research Foundation, Salt Lake City Vorrichtung und Verfahren zur Durchführung und Überprüfung von biologischen Prozessen
CA2257109C (en) 1996-06-04 2009-10-06 University Of Utah Research Foundation Monitoring hybridization during pcr
US6787305B1 (en) 1998-03-13 2004-09-07 Invitrogen Corporation Compositions and methods for enhanced synthesis of nucleic acid molecules
US20030118992A1 (en) 1998-09-29 2003-06-26 Warren Richard Lloyd ABC transporter
DE19913923A1 (de) 1999-03-26 2000-09-28 Amphenol Tuchel Elect Kontaktiereinrichtung für eine SIM-Karte
WO2000068411A1 (en) 1999-05-12 2000-11-16 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
DE19929705A1 (de) * 1999-06-29 2001-01-04 Biotronik Mess & Therapieg Verfahren zur Bestimmung der Baroreflex-Latenzzeit und Baroreflex-Sensitivität
CA2417986C (en) 2000-08-11 2013-11-26 University Of Utah Research Foundation Single-labeled oligonucleotide probes
EP1377683A4 (en) * 2001-04-11 2004-09-08 Us Gov Health & Human Serv MODIFIED RANDOM SPRAYERS FOR PROBE MARKING
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US6403341B1 (en) 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
US6617137B2 (en) * 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA

Also Published As

Publication number Publication date
ES2450073T3 (es) 2014-03-21
CA2658520C (en) 2016-11-08
US20090269766A1 (en) 2009-10-29
EP2103693A1 (en) 2009-09-23
CN101691593B (zh) 2015-11-25
JP5457055B2 (ja) 2014-04-02
CN101691593A (zh) 2010-04-07
EP2103693B1 (en) 2013-12-11
US8592184B2 (en) 2013-11-26
US20130109060A1 (en) 2013-05-02
JP2009225796A (ja) 2009-10-08
CA2658520A1 (en) 2009-09-19

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