HK1130837A1 - Fen-1 endonucleases, mixtures and cleavage methods fen-1 - Google Patents

Fen-1 endonucleases, mixtures and cleavage methods fen-1

Info

Publication number
HK1130837A1
HK1130837A1 HK09108931.6A HK09108931A HK1130837A1 HK 1130837 A1 HK1130837 A1 HK 1130837A1 HK 09108931 A HK09108931 A HK 09108931A HK 1130837 A1 HK1130837 A1 HK 1130837A1
Authority
HK
Hong Kong
Prior art keywords
fen
nucleic acid
acid sequences
endonucleases
mixtures
Prior art date
Application number
HK09108931.6A
Other languages
English (en)
Inventor
Michael W Kaiser
Victor I Lyamichev
Natasha Lyamicheva
Original Assignee
Third Wave Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/757,653 external-priority patent/US5843669A/en
Priority claimed from US08/758,314 external-priority patent/US6090606A/en
Application filed by Third Wave Tech Inc filed Critical Third Wave Tech Inc
Publication of HK1130837A1 publication Critical patent/HK1130837A1/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
HK09108931.6A 1996-11-29 2009-09-28 Fen-1 endonucleases, mixtures and cleavage methods fen-1 HK1130837A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/757,653 US5843669A (en) 1996-01-24 1996-11-29 Cleavage of nucleic acid acid using thermostable methoanococcus jannaschii FEN-1 endonucleases
US08/758,314 US6090606A (en) 1996-01-24 1996-12-02 Cleavage agents

Publications (1)

Publication Number Publication Date
HK1130837A1 true HK1130837A1 (en) 2010-01-08

Family

ID=27116427

Family Applications (2)

Application Number Title Priority Date Filing Date
HK09108931.6A HK1130837A1 (en) 1996-11-29 2009-09-28 Fen-1 endonucleases, mixtures and cleavage methods fen-1
HK12103400.4A HK1163158A1 (en) 1996-11-29 2012-04-05 Fen-1 endonucleases, mixtures and cleavage methods fen-1

Family Applications After (1)

Application Number Title Priority Date Filing Date
HK12103400.4A HK1163158A1 (en) 1996-11-29 2012-04-05 Fen-1 endonucleases, mixtures and cleavage methods fen-1

Country Status (11)

Country Link
US (2) US6562611B1 (fr)
EP (3) EP2055781B1 (fr)
JP (4) JP4362150B2 (fr)
AT (2) ATE414167T1 (fr)
AU (1) AU737449B2 (fr)
CA (1) CA2273204C (fr)
DE (1) DE69739108D1 (fr)
DK (1) DK0966542T3 (fr)
ES (2) ES2375764T3 (fr)
HK (2) HK1130837A1 (fr)
WO (1) WO1998023774A1 (fr)

Families Citing this family (96)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7150982B2 (en) * 1991-09-09 2006-12-19 Third Wave Technologies, Inc. RNA detection assays
US6759226B1 (en) 2000-05-24 2004-07-06 Third Wave Technologies, Inc. Enzymes for the detection of specific nucleic acid sequences
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5985557A (en) * 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
US6780982B2 (en) 1996-07-12 2004-08-24 Third Wave Technologies, Inc. Charge tags and the separation of nucleic acid molecules
JP4362150B2 (ja) * 1996-11-29 2009-11-11 サード ウェーブ テクノロジーズ，インコーポレーテッド Ｆｅｎ−１エンドヌクレアーゼ、混合物、および開裂方法
EP0983292A4 (fr) 1997-05-05 2005-05-18 Third Wave Tech Inc Reactions dependantes des cibles, utilisant des oligonucleotides de pontage de structures
US6210880B1 (en) 1997-09-19 2001-04-03 Third Wave Technologies, Inc. Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides
US6194149B1 (en) 1998-03-03 2001-02-27 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
US7101672B2 (en) 1998-05-05 2006-09-05 Third Wave Technologies, Inc. Target-dependent reactions using structure-bridging oligonucleotides
EP1185544B1 (fr) 1999-05-24 2008-11-26 Invitrogen Corporation Procede de deblocage d'oligonucleotides marques
US7838225B2 (en) 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US7534568B2 (en) 1999-10-29 2009-05-19 Hologic Inc. Methods for detection of a target nucleic acid by forming a cleavage structure with a cleavage resistant probe
US7118860B2 (en) 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
WO2001061015A2 (fr) * 2000-02-17 2001-08-23 Qiagen Gmbh Nouvelles acide nucleique polymerases chimeriques thermostables
US6500622B2 (en) * 2000-03-22 2002-12-31 Quantum Dot Corporation Methods of using semiconductor nanocrystals in bead-based nucleic acid assays
ATE296899T1 (de) 2000-03-27 2005-06-15 Applera Corp Verbesserter invasionsassay
EP1294863B1 (fr) * 2000-05-24 2009-07-08 Third Wave Technologies, Inc. Detection d'arn
DE60125243T2 (de) 2000-07-03 2007-05-31 Applera Corp., Foster City Polynukleotid-sequenzassay
DE10049211A1 (de) * 2000-10-05 2002-04-18 Qiagen Gmbh Thermostabile Polymerase aus Thermococcus pacificus
US6350580B1 (en) 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure
AU2001297524A1 (en) * 2000-11-15 2002-09-19 Third Wave Technologies, Inc. Fen endonucleases
DE10131441A1 (de) * 2001-06-29 2003-01-30 Henkel Kgaa Eine neue Gruppe von alpha-Amylasen sowie ein Verfahren zur Identifizierung und Gewinnung neuer alpha-Amylasen
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US7312033B2 (en) 2002-11-14 2007-12-25 Third Wave Technologies, Inc. CFTR allele detection assays
EP1573009B1 (fr) 2002-12-18 2011-09-21 Third Wave Technologies, Inc. Detection de petits acides nucleiques
US8206904B2 (en) 2002-12-18 2012-06-26 Third Wave Technologies, Inc. Detection of nucleic acids
US7700750B2 (en) 2003-01-09 2010-04-20 Third Wave Technologies, Inc. Connexin allele detection assays
US20040161749A1 (en) 2003-01-24 2004-08-19 Hall Jeff G. 3' end tagged oligonucleotides
ATE544852T1 (de) 2003-07-29 2012-02-15 Otsuka Pharma Co Ltd Verfahren zur beurteilung der gefahr arzneimittelinduzierter granulozytopenie
US7473773B2 (en) 2004-01-07 2009-01-06 Third Wave Technologies, Inc. Determination of hepatitis C virus genotype
US7309589B2 (en) * 2004-08-20 2007-12-18 Vironix Llc Sensitive detection of bacteria by improved nested polymerase chain reaction targeting the 16S ribosomal RNA gene and identification of bacterial species by amplicon sequencing
EP1813673B1 (fr) 2004-10-28 2011-05-11 Otsuka Pharmaceutical Co., Ltd. Marqueur d identification sensible a la therapie aux interferons dans le traitement de cancers des cellules renales
US7727721B2 (en) 2005-03-08 2010-06-01 California Institute Of Technology Hybridization chain reaction amplification for in situ imaging
US7960357B2 (en) 2005-10-07 2011-06-14 California Institute Of Technology PKR activation via hybridization chain reaction
US7482127B2 (en) 2005-11-03 2009-01-27 Third Wave Technologies, Inc. Nucleic acid detection assays employing blocker oligonucleotides
WO2007111324A1 (fr) * 2006-03-29 2007-10-04 Kyoto University Procédé de détection de la 5-méthylcytosine dans un gène et trousse de détection
CN103146825B (zh) 2006-06-01 2017-08-25 第三次浪潮技术公司 核酸的检测
US7759062B2 (en) 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
EP2079754B1 (fr) 2006-10-04 2017-03-15 Third Wave Technologies, Inc. Amorces snap-back et structures en epingle a cheveux detectables
EP2155770B1 (fr) * 2007-05-16 2013-10-16 California Institute of Technology Motif polyvalent d'acide nucléique en épingle à cheveux pour la programmation de voies d'auto-assemblage biomoléculaire
US8497364B2 (en) 2008-02-27 2013-07-30 California Institute Of Technology Triggered RNAi
AU2009225835B2 (en) 2008-03-15 2013-02-14 Hologic, Inc. Compositions and methods for analysis of nucleic acid molecules during amplification reactions
US20090246788A1 (en) 2008-04-01 2009-10-01 Roche Nimblegen, Inc. Methods and Assays for Capture of Nucleic Acids
US20110136118A1 (en) * 2008-06-17 2011-06-09 Sigma-Aldrich Co. Real time polymerase chain reaction process using a universal detection system
US8658780B2 (en) 2010-05-18 2014-02-25 California Institute Of Technology Triggered covalent probes for imaging and silencing genetic expression
US9834439B2 (en) 2010-07-20 2017-12-05 California Institute Of Technology Biomolecular self-assembly
US8877438B2 (en) 2010-07-20 2014-11-04 California Institute Of Technology Self-assembled polynucleotide structure
US8962241B2 (en) 2010-07-20 2015-02-24 California Institute Of Technology Triggered molecular geometry based bioimaging probes
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
US8715937B2 (en) 2010-11-15 2014-05-06 Exact Sciences Corporation Mutation detection assay
WO2012096430A1 (fr) 2011-01-11 2012-07-19 Seegene, Inc. Détection de séquences d'acide nucléique cibles par dosage de clivage et d'extension pto
KR101569479B1 (ko) 2011-03-29 2015-11-16 주식회사 씨젠 Ｐｔｏ 절단 및 연장-의존적 절단에 의한 타겟 핵산서열의 검출
US9850524B2 (en) 2011-05-04 2017-12-26 Seegene, Inc. Detection of target nucleic acid sequences by PO cleavage and hybridization
CA2849020C (fr) 2011-10-18 2023-05-09 Exact Sciences Corporation Dosage multiplex pour la detection de mutations kras
KR20130101952A (ko) 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
RU2620955C2 (ru) * 2012-03-05 2017-05-30 Сиджен, Инк. Детекция нуклеотидной вариации в нуклеиновокислотной последовательности-мишени в анализе с расщеплением и удлинением зондирующего и метящего олигонуклеотида (рто)
WO2014158628A1 (fr) 2013-03-14 2014-10-02 Hologic, Inc. Compositions et procédés d'analyse de molécules d'acide nucléique
US9856472B2 (en) 2013-07-01 2018-01-02 California Institute Of Technology Small conditional RNAs
PL3030682T3 (pl) 2013-08-05 2020-11-16 Twist Bioscience Corporation Biblioteki genowe syntezowane de novo
WO2016126882A1 (fr) 2015-02-04 2016-08-11 Twist Bioscience Corporation Procédés et dispositifs pour assemblage de novo d'acide oligonucléique
WO2016172377A1 (fr) 2015-04-21 2016-10-27 Twist Bioscience Corporation Dispositifs et procédés pour la synthèse de banques d'acides oligonucléiques
US20180163259A1 (en) 2015-05-01 2018-06-14 Gen-Probe Incorporated Multiplex invasive cleavage assays
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
US11512347B2 (en) 2015-09-22 2022-11-29 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
EP3943613A1 (fr) 2016-07-05 2022-01-26 California Institute of Technology Réaction en chaîne d'hybridation d'initiateurs fractionnaire
AU2017315294B2 (en) 2016-08-22 2023-12-21 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
IL264831B (en) 2016-08-30 2022-09-01 California Inst Of Techn Immunohistochemistry by hybridization chain reaction
JP6871364B2 (ja) 2016-09-21 2021-05-12 ツイスト バイオサイエンス コーポレーション 核酸に基づくデータ保存
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
JP2020508661A (ja) 2017-02-22 2020-03-26 ツイスト バイオサイエンス コーポレーション 核酸ベースのデータ保存
WO2018170169A1 (fr) 2017-03-15 2018-09-20 Twist Bioscience Corporation Banques de variants de la synapse immunologique et leur synthèse
CN107012241B (zh) * 2017-05-09 2020-11-20 东莞微量精准检测研究院有限公司 一种U6、miR 92a及miR 21的三重RT-qPCR检测方法及试剂盒
WO2018231864A1 (fr) 2017-06-12 2018-12-20 Twist Bioscience Corporation Méthodes d'assemblage d'acides nucléiques continus
KR102628876B1 (ko) 2017-06-12 2024-01-23 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
CN118326009A (zh) * 2017-07-31 2024-07-12 凸版印刷株式会社 核酸检测方法及检测用试剂
EP4219766A3 (fr) 2017-08-11 2023-10-11 Gen-Probe Incorporated Compositions et procédés de détection de staphylococcus aureus
KR20200047706A (ko) 2017-09-11 2020-05-07 트위스트 바이오사이언스 코포레이션 Gpcr 결합 단백질 및 이의 합성 방법
KR102345601B1 (ko) 2017-09-29 2021-12-30 주식회사 씨젠 Pto 절단 및 연장-의존적 연장 분석에 의한 타겟 핵산 서열의 검출
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
WO2019118550A1 (fr) 2017-12-13 2019-06-20 Gen-Probe Incorporated Procédés de traitement d'un échantillon biologique
WO2019118735A1 (fr) 2017-12-15 2019-06-20 Gen-Probe Incorporated Compositions et méthodes pour la détection de clostridium difficile toxigène
CA3088911A1 (fr) 2018-01-04 2019-07-11 Twist Bioscience Corporation Dispositif de stockage a base d'adn et methode de synthese de polynucleotides utilisant le dispositif
AU2019270243A1 (en) 2018-05-18 2021-01-07 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
EP3914732A1 (fr) 2019-01-25 2021-12-01 Gen-Probe Incorporated Détection de mycoplasma genitalium résistant aux médicaments
CA3131691A1 (fr) 2019-02-26 2020-09-03 Twist Bioscience Corporation Banques d'acides nucleiques variants pour l'optimisation d'anticorps
AU2020229349A1 (en) 2019-02-26 2021-10-14 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021061829A1 (fr) 2019-09-23 2021-04-01 Twist Bioscience Corporation Banques d'acides nucléiques variants pour crth2
EP4284925A1 (fr) 2021-01-26 2023-12-06 California Institute of Technology Arn guides conditionnels allostériques pour la régulation sélective de cellules de crispr/cas
JP2024537076A (ja) 2021-09-30 2024-10-10 ジェン－プローブ・インコーポレーテッド 温度で選択可能なｆｒｅｔカセットシグナル伝達
WO2024054924A1 (fr) 2022-09-08 2024-03-14 Gen-Probe Incorporated Procédé de détection d'analytes d'acides nucléiques à l'aide d'amorces à double spécificité

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4511502A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4518526A (en) 1982-12-22 1985-05-21 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4512922A (en) 1982-12-22 1985-04-23 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4683194A (en) 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US5118605A (en) 1984-10-16 1992-06-02 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4775619A (en) 1984-10-16 1988-10-04 Chiron Corporation Polynucleotide determination with selectable cleavage sites
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4876187A (en) 1985-12-05 1989-10-24 Meiogenics, Inc. Nucleic acid compositions with scissile linkage useful for detecting nucleic acid sequences
US5011769A (en) 1985-12-05 1991-04-30 Meiogenics U.S. Limited Partnership Methods for detecting nucleic acid sequences
WO1992006200A1 (fr) 1990-09-28 1992-04-16 F. Hoffmann-La-Roche Ag Mutations d'adn-polymerases thermostables en 5' a 3' exonuclease
US5403711A (en) 1987-11-30 1995-04-04 University Of Iowa Research Foundation Nucleic acid hybridization and amplification method for detection of specific sequences in which a complementary labeled nucleic acid probe is cleaved
JP2856804B2 (ja) 1988-03-24 1999-02-10 ユニバーシティ オブ アイオワ リサーチ ファウンデイション 標識された相補的核酸プローブを開裂する触媒反応の補因子としての活性に基づく核酸配列検出のための触媒ハイブリッド形成系
WO1990001069A1 (fr) 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Procede d'amplification et de detection de sequences d'acide nucleique
WO1990010691A1 (fr) 1989-03-10 1990-09-20 Grace-Sierra Horticultural Products Company Culture de tissus
US5144019A (en) 1989-06-21 1992-09-01 City Of Hope Ribozyme cleavage of HIV-I RNA
CA1337639C (fr) 1989-08-01 1995-11-28 Joseph Eugene Celebuski Essai de sondes d'adn faisant appel a des brins de sondes ayant une charge neutre
US5108892A (en) 1989-08-03 1992-04-28 Promega Corporation Method of using a taq dna polymerase without 5'-3'-exonuclease activity
ATE169337T1 (de) 1989-12-22 1998-08-15 Hoffmann La Roche Rekombinante expressionsvektoren und reinigungsverfahren für dns-polymerase aus thermus thermophilus
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US6083686A (en) 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5994069A (en) 1996-01-24 1999-11-30 Third Wave Technologies, Inc. Detection of nucleic acids by multiple sequential invasive cleavages
US5541311A (en) * 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5888780A (en) 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
US5422253A (en) 1992-12-07 1995-06-06 Wisconsin Alumni Research Foundation Method of site specific nucleic acid cleavage
AU8102694A (en) 1993-11-17 1995-06-06 Id Biomedical Corporation Cycling probe cleavage detection of nucleic acid sequences
CA2188660C (fr) 1994-04-25 2005-01-18 Richard G. H. Cotton Detection de mutations par clivage par resolvase
EP0705905B1 (fr) 1994-07-16 2001-10-10 Roche Diagnostics GmbH Procédé pour la détection sensible d'acides nucléiques
AU4234796A (en) 1994-11-09 1996-06-06 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants and pathogens
DE4441626A1 (de) 1994-11-23 1996-05-30 Boehringer Mannheim Gmbh Verfahren zum besonders sensitiven Nachweis von Nukleinsäuren
US5545729A (en) 1994-12-22 1996-08-13 Hybridon, Inc. Stabilized ribozyme analogs
WO1996020287A2 (fr) 1994-12-23 1996-07-04 Behringwerke Aktiengesellschaft Detection d'acides nucleiques par formation d'un produit catalyse par une cible
US5874283A (en) 1995-05-30 1999-02-23 John Joseph Harrington Mammalian flap-specific endonuclease
US5882867A (en) 1995-06-07 1999-03-16 Dade Behring Marburg Gmbh Detection of nucleic acids by formation of template-dependent product
US5985557A (en) 1996-01-24 1999-11-16 Third Wave Technologies, Inc. Invasive cleavage of nucleic acids
DK1634890T3 (da) * 1996-01-24 2009-03-09 Third Wave Tech Inc Invasiv klövning af nukleinsyrer
JP4362150B2 (ja) * 1996-11-29 2009-11-11 サード ウェーブ テクノロジーズ，インコーポレーテッド Ｆｅｎ−１エンドヌクレアーゼ、混合物、および開裂方法

Also Published As

Publication number Publication date
DK0966542T3 (da) 2009-03-23
JP4191228B2 (ja) 2008-12-03
JP2008206521A (ja) 2008-09-11
DE69739108D1 (de) 2008-12-24
EP0966542A1 (fr) 1999-12-29
JP2007259869A (ja) 2007-10-11
EP0966542A4 (fr) 2004-04-14
HK1163158A1 (en) 2012-09-07
EP2374890A2 (fr) 2011-10-12
CA2273204A1 (fr) 1998-06-04
ES2317658T3 (es) 2009-04-16
ATE414167T1 (de) 2008-11-15
EP0966542B1 (fr) 2008-11-12
ES2375764T3 (es) 2012-03-06
AU5589898A (en) 1998-06-22
US6555357B1 (en) 2003-04-29
EP2055781A3 (fr) 2009-10-21
JP4362150B2 (ja) 2009-11-11
EP2055781A2 (fr) 2009-05-06
CA2273204C (fr) 2008-10-14
JP2009297032A (ja) 2009-12-24
AU737449B2 (en) 2001-08-23
ATE530652T1 (de) 2011-11-15
EP2374890B1 (fr) 2012-11-14
EP2374890A3 (fr) 2011-11-09
EP2055781B1 (fr) 2011-10-26
JP2001526526A (ja) 2001-12-18
US6562611B1 (en) 2003-05-13
WO1998023774A1 (fr) 1998-06-04

Similar Documents

Publication Publication Date Title
HK1163158A1 (en) Fen-1 endonucleases, mixtures and cleavage methods fen-1
EP0904286A4 (fr) Clivage invasif d'acides nucleiques
WO2002070755A3 (fr) Endonucleases fen
WO2001090337A3 (fr) Detection d'arn
EP2058407A3 (fr) Division invasive d'acides nucléiques
ATE274587T1 (de) 5'-nukleasen aus thermostabilen dna-polymerasen
HK1081593A1 (en) Rna detection enzymes
CA2284552A1 (fr) Detection d'acides nucleique par clivages sequentiels invasifs multiples
WO1997026270A3 (fr) Synthese de methoxy nucleosides et de molecules d'acide nucleique enzymatique
BR9407712A (pt) Métodos e composiçoes para sequenciamento eficaz de ácidos nuclêicos
HUT77576A (hu) Enzimhatású DNS-molekulák
EP0266787A3 (fr) Procédé pour la détection des polymorphismes de restriction-longueur de fragment dans les génomes eucaryotiques
ATE326549T1 (de) Spezifische bestimmung der methylierung
ID16617A (id) Proses penggilingan basah dan penggiling untuk melakukan proses tersebut
GB9510954D0 (en) Detection of DNA damage
CA2348776A1 (fr) Ligase thermostable haute fidelite et ses utilisations
WO1991009942A3 (fr) Endonuclease d'adn bicatenaire specifique de site
UA70937C2 (uk) Спосіб кількісної оцінки реакції в твердій фазі та фторовмісний компонент реакції
WO1999043845A3 (fr) Procede de sequençage de polynucleotides et kits associes

Legal Events

Date Code Title Description
PE Patent expired

Effective date: 20171125