WO1991009942A3 - Endonuclease d'adn bicatenaire specifique de site - Google Patents

Endonuclease d'adn bicatenaire specifique de site

Info

Publication number
WO1991009942A3
WO1991009942A3 PCT/US1990/007644 US9007644W WO9109942A3 WO 1991009942 A3 WO1991009942 A3 WO 1991009942A3 US 9007644 W US9007644 W US 9007644W WO 9109942 A3 WO9109942 A3 WO 9109942A3
Authority
WO
WIPO (PCT)
Prior art keywords
double stranded
stranded dna
site
endonuclease
dna endonuclease
Prior art date
Application number
PCT/US1990/007644
Other languages
English (en)
Other versions
WO1991009942A2 (fr
Inventor
Ronald A Butow
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Publication of WO1991009942A2 publication Critical patent/WO1991009942A2/fr
Publication of WO1991009942A3 publication Critical patent/WO1991009942A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

On a isolé, purifié et caractérisé une endonucléase clivant de l'ADN bicaténaire au niveau d'un site spécifique, produisant des extrémités échlonnées comportant des surplans en position 3' présentant 4 paires de base. L'invention concerne également un procédé de production de quantités suffisantes de l'endonucléase déstinée à une purification et une caractérisation. L'invention concerne en outre un procédé d'utilisation de l'endonucléase afin de cliver l'ADN, produisant des fragments utiles à la cartographie des gènes.
PCT/US1990/007644 1989-12-22 1990-12-24 Endonuclease d'adn bicatenaire specifique de site WO1991009942A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45610089A 1989-12-22 1989-12-22
US456,100 1989-12-22

Publications (2)

Publication Number Publication Date
WO1991009942A2 WO1991009942A2 (fr) 1991-07-11
WO1991009942A3 true WO1991009942A3 (fr) 1991-08-22

Family

ID=23811425

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1990/007644 WO1991009942A2 (fr) 1989-12-22 1990-12-24 Endonuclease d'adn bicatenaire specifique de site

Country Status (3)

Country Link
US (1) US5310664A (fr)
AU (1) AU7145991A (fr)
WO (1) WO1991009942A2 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5792632A (en) * 1992-05-05 1998-08-11 Institut Pasteur Nucleotide sequence encoding the enzyme I-SceI and the uses thereof
CA2520415A1 (fr) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. Procedes d'identification de composes ciblant une endonuclease d'epissage d'arnt et utilisations desdits composes comme agents anti-proliferatifs
CA2520510A1 (fr) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc. Procedes d'identification de composes ciblant l'endonuclease d'epissage d'arnt et utilisations desdits composes comme agents antifongiques
JP2006521116A (ja) * 2003-03-27 2006-09-21 ピーティーシー セラピューティクス，インコーポレーテッド 抗真菌性および／または抗増殖性分子を同定するためのｔＲＮＡスプライシング経路の酵素の標的化
US20050053985A1 (en) * 2003-07-02 2005-03-10 Ptc Therapeutics, Inc. RNA processing protein complexes and uses thereof

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3512435A1 (de) * 1984-06-13 1986-01-02 Boehringer Mannheim Gmbh, 6800 Mannheim Restriktionsendonuklease asp 718, ihre gewinnung und verwendung
DE3425957A1 (de) * 1984-07-13 1986-01-16 Boehringer Mannheim Gmbh, 6800 Mannheim Restriktionsendonuklease dra iii, ihre gewinnung und verwendung
US4935367A (en) * 1988-03-17 1990-06-19 The United States Of America As Represented By The Department Of Health And Human Services Novel restriction endonuclease

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Cell, vol. 56, no. 3, 10 February 1989, Cell Press, (Cambridge, Mass., US), A. Delahodde et al.: "Site-specific DNA endonuclease and RNA maturase activities of two homologous intron-encoded proteins from yeast mitochondria", pages 431-441 *
Cell, vol. 56, no. 3, 10 February 1989, Cell Press, (Cambridge, Mass., US), J.M. Wenzlau et al.: "A latent intron-encoded maturase is also an endonuclease needed for intron mobility", pages 421-430 *
Nucleic Acids Research, vol. 18, no. 19, B. Sargueil et al.: "In vivo and in vitro analyses of an intron-encoded DNA endonuclease from yeast mitochondria. Recognition site by site-directed mutagenesis", pages 5659-5665 *
The Journal of Biological Chemistry,vol.265,no.31,November 1990,The American Society for Biochemistry and Molecular Biology,Inc.,(US),C.M.Wernette et al.:"Purification of a site-specific endonuclease,I-Sce II,encoded by intron 4alpha of the mitochondrial coxI gene of Saccharomyces cerevisiae". *

Also Published As

Publication number Publication date
WO1991009942A2 (fr) 1991-07-11
AU7145991A (en) 1991-07-24
US5310664A (en) 1994-05-10

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