HK1125672A1 - Isolation and purification of nucleic acid molecules with a solid phase - Google Patents

Isolation and purification of nucleic acid molecules with a solid phase

Info

Publication number
HK1125672A1
HK1125672A1 HK09103475.9A HK09103475A HK1125672A1 HK 1125672 A1 HK1125672 A1 HK 1125672A1 HK 09103475 A HK09103475 A HK 09103475A HK 1125672 A1 HK1125672 A1 HK 1125672A1
Authority
HK
Hong Kong
Prior art keywords
isolation
purification
nucleic acid
solid phase
acid molecules
Prior art date
Application number
HK09103475.9A
Other languages
English (en)
Inventor
Christian Birkner
Herbert Von Der Eltz
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1125672A1 publication Critical patent/HK1125672A1/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
HK09103475.9A 2007-04-20 2009-04-15 Isolation and purification of nucleic acid molecules with a solid phase HK1125672A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP07008073 2007-04-20

Publications (1)

Publication Number Publication Date
HK1125672A1 true HK1125672A1 (en) 2009-08-14

Family

ID=38290130

Family Applications (1)

Application Number Title Priority Date Filing Date
HK09103475.9A HK1125672A1 (en) 2007-04-20 2009-04-15 Isolation and purification of nucleic acid molecules with a solid phase

Country Status (6)

Country Link
US (1) US8101744B2 (de)
EP (1) EP1983051B1 (de)
JP (2) JP5325448B2 (de)
CN (1) CN101289661B (de)
CA (1) CA2629589C (de)
HK (1) HK1125672A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2381279T3 (es) * 2005-05-06 2012-05-24 Gen-Probe Incorporated Métodos y productos para la captura de ácido nucleico diana
CA2723726C (en) 2008-05-13 2017-09-12 Michael M. Becker Inactivatable target capture oligomers for use in the selective hybridization and capture of target nucleic acid sequences
US20120141980A1 (en) * 2009-08-17 2012-06-07 Merk Patent Gesellschaft Mit Beschrankter Haftung Method for isolating viruses
US9371509B2 (en) * 2009-09-02 2016-06-21 Roche Diagnostics Operations, Inc. Reagents for lysis of bacterial cells
US8420801B2 (en) 2010-01-08 2013-04-16 Roche Molecular Systems, Inc. Recovery of nucleic acids from magnetic glass particles
ES2543367T3 (es) 2010-07-07 2015-08-18 Diagon Kft. Procedimiento para el aislamiento específico del contenido de ADN total de gérmenes bacterianos y un kit para dicho fin
US20150045232A1 (en) * 2010-12-28 2015-02-12 Bexmart Integrated and versatile methods for systems diagnosis of diseases
CN102533725A (zh) * 2011-09-09 2012-07-04 上海佑科生物技术有限公司 一体化缓冲液及使用该一体化缓冲液分离核酸的方法
US9340828B2 (en) 2011-10-27 2016-05-17 Ge Healthcare Bio-Sciences Ab Purification of nucleic acid
GB201303666D0 (en) * 2013-03-01 2013-04-17 Goldsborough Andrew S Sample fixation and stabilisation
RU2016132972A (ru) 2014-01-28 2018-03-05 Дайс Молекьюлс СВ, ЭлЭлСи Монолиты с присоединенными распознающими соединениями, их система и их применение
JP2016020826A (ja) * 2014-07-14 2016-02-04 山善株式会社 分取液体クロマトフラフィ用インジェクタ
US20200063189A1 (en) * 2017-02-27 2020-02-27 miDiagnostics NV System and method for purifying and amplifying nucleic acids
CN115667510A (zh) * 2020-05-25 2023-01-31 积水医疗株式会社 核酸的纯化方法
CN114573483B (zh) * 2022-03-16 2022-11-01 湖南师范大学 一种疏水的磁性离子液体及其制备方法和应用
CN114990109A (zh) * 2022-06-21 2022-09-02 中国科学院过程工程研究所 一种核糖核酸纯化伴侣及其应用
CN115960885B (zh) * 2022-10-09 2023-12-12 南京诺唯赞生物科技股份有限公司 一种提取肝素钠样品中核酸的方法及组合物

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
ATE107654T1 (de) 1987-03-02 1994-07-15 Gen Probe Inc Polykationische träger zur reinigung, trennung und hybridisierung von nukleinsäure.
DE3724442A1 (de) 1987-07-23 1989-02-02 Europ Lab Molekularbiolog Verfahren und vorrichtung zur reinigung von m-13-phagen-dna
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
EP0425563B1 (de) 1988-07-20 1996-05-15 David Segev Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
ES2089038T3 (es) 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
JPH06501225A (ja) 1990-07-09 1994-02-10 ジェイ ガズデン ピーティーワイ リミテッド 改良型容器
DE69433425T2 (de) * 1993-08-30 2004-10-07 Promega Corp Zusammensetzungen und verfahren zur reinigung von nukleinsäuren
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE69734263T2 (de) 1996-07-12 2006-07-13 Toyo Boseki K.K. Verfahren zur Isolierung von Ribonucleinsäuren.
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
GB9725839D0 (en) * 1997-12-06 1998-02-04 Baker Matthew J Isolation of nucleic acids
CN1230440C (zh) * 1997-12-06 2005-12-07 Dna研究创新有限公司 核酸的分离
EP0969090A1 (de) 1998-05-27 2000-01-05 QIAGEN GmbH Schnelles und einfaches Verfahren zur Isolierung von zirkulären Nucleinsäuren
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
JP2003535589A (ja) 2000-06-02 2003-12-02 パル・コーポレーション プラスミド含有液のプロセシング
US6858720B2 (en) * 2001-10-31 2005-02-22 Agilent Technologies, Inc. Method of synthesizing polynucleotides using ionic liquids
US20040121336A1 (en) * 2002-12-20 2004-06-24 Greenfield I Lawrence Method for generating multiple samples containing a predetermined amount of nucleic acid
US20040180445A1 (en) * 2003-03-12 2004-09-16 Domanico Michael J. Methods and compositions for purification of nucleic acid from a host cell
WO2005064010A1 (de) 2003-12-19 2005-07-14 Preanalytix Gmbh Zusammensetzung zum binden von nukleinsäure an eine festphase
AU2005200670B2 (en) * 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
JPWO2005090563A1 (ja) 2004-03-23 2008-02-07 大野 弘幸 核酸溶解用溶媒、核酸含有溶液および核酸保存方法
BRPI0621413A2 (pt) * 2006-02-13 2011-12-06 Agency Science Tech & Res método de processamento de uma amostra biológica e/ou quìmica

Also Published As

Publication number Publication date
JP2012255798A (ja) 2012-12-27
EP1983051A3 (de) 2008-12-03
CN101289661B (zh) 2013-08-21
JP2008263982A (ja) 2008-11-06
JP5325448B2 (ja) 2013-10-23
JP5685227B2 (ja) 2015-03-18
US20080319182A1 (en) 2008-12-25
US8101744B2 (en) 2012-01-24
CA2629589C (en) 2016-03-29
EP1983051A2 (de) 2008-10-22
CN101289661A (zh) 2008-10-22
EP1983051B1 (de) 2014-11-26
CA2629589A1 (en) 2008-10-20

Similar Documents

Publication Publication Date Title
HK1125672A1 (en) Isolation and purification of nucleic acid molecules with a solid phase
IL204892A0 (en) Assembly of large nucleic acids
EP2268813A4 (de) Rna-moleküle und ihre verwendung
WO2010117456A9 (en) Column enrichment of pcr beads comprising tethered amplicons
GB0711675D0 (en) Labelling of RNA
EP2585595B8 (de) Rna-moleküle und ihre verwendung
EP2114831A4 (de) Reinigungsverfahren für eine fluorinbasierte lösungsmittelhaltige lösung
HK1143323A1 (zh) 採用 結合分子的聯合療法
GB0706243D0 (en) Modified nucleic acids
GB0814570D0 (en) Isolation of nucleic acid
ZA200904559B (en) Truncated alternan sucrase coding nucleic acid molecules
EP2126072A4 (de) Materialien und verfahren zur einzelmolekülsequenzierung von nukleinsäuren
EP2272856A4 (de) Sequenzspezifisches verfahren zur aufreinigung von nukleinsäure
EP1996730A4 (de) Reinigung der kleinen rna
ZA201001043B (en) A gas reformulation system comprising means to optimize the effectiveness of gas convertion
EP2318508A4 (de) Bestimmung der integrität einer rna
EP2118297A4 (de) Erzeugung von nukleinsäuremolekülen
EP2137309A4 (de) Verfahren zur trennung biologischer moleküle unter verwendung von sulfolan
EP2473614A4 (de) K5-heparosan-fermentierung und -reinigung
EP2455469A4 (de) Zur bindung an hmgb1 fähiges nukleinsäuremolekül und verwendung davon
HK1125112A1 (en) Adsorption of nucleic acids to solid phases under low-salt conditions
GB2452975B (en) Formation of biodiesel using microorganisms
GB0903562D0 (en) RNA molecules
EP2488644A4 (de) Epigenetische dna-anreicherung
GB0707790D0 (en) Nucleic acid-containing media