GR1000492B - Κλωνισμος και εκφραση μετασχηματιστικου παραγοντα αναπτυξης β2. - Google Patents

Κλωνισμος και εκφραση μετασχηματιστικου παραγοντα αναπτυξης β2.

Info

Publication number
GR1000492B
GR1000492B GR880100660A GR880100660A GR1000492B GR 1000492 B GR1000492 B GR 1000492B GR 880100660 A GR880100660 A GR 880100660A GR 880100660 A GR880100660 A GR 880100660A GR 1000492 B GR1000492 B GR 1000492B
Authority
GR
Greece
Prior art keywords
tgf
cells
mature
cdna
tamoxifen
Prior art date
Application number
GR880100660A
Other languages
English (en)
Inventor
Madisen Linda
Webb Nancy
F Purchio Antony
Original Assignee
Oncogen Etairia Periorismenis
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncogen Etairia Periorismenis filed Critical Oncogen Etairia Periorismenis
Publication of GR1000492B publication Critical patent/GR1000492B/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/495—Transforming growth factor [TGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Περιγράφονται cDNA κλώνοι κωδικοποιητικοί για TGF-β2 που χρησιμοποιούνται για να κατασκευάσουν φορείς έκφρασης ικανούς να κατευθύνουν την υψηλού επιπέδου έκφραση ώριμου βιολογικά δραστικού TGF-β2 σε κύτταρα ωοθήκης Κινεζικού Hamster, που έχουν υποστεί διατομή (CHO κύτταρα). Ο TGF-β2 που παράγεται και εκκρίνεται από παράγοντες διατομής CHO, έχουν ειδική δραστηριότητα που υπολογίζεται να είναι περίπου η μισή εκείνης του ανασυνδυαζόμενου TGF--β1. Οι κλώνοι απομονώθηκαν από μια κυτταρική γραμμή ανθρώπινου προστατικού αδενοκαρκινώματος που είχε υποστεί αγωγή με Tamoxifen (PC-3) με τη χρήση ολιγονουκλεότιδο διερευνητών επί τη βάσει της μερικής αλληλουχίας αμινοξέος καθαρισμένου TGF-β2, από μία κυτταρική γραμμή Αφρικανικού πράσινου πιθήκου, την BSC-40. Οι cDNA αλληλουχίες, προβλέπουν ότι ο TGF-β2 συντίθεται σαν μορφές προδρόμου πολυπεπτιδίου από τις οποίες λαμβάνεται με πρωτεολυτικό τεμαχισμό η ώριμη 112 αμινοξέος TGF-β2 υπομονάδα. Οι πρωτεϊνες που κωδικοποιούνται από τα ανθρώπινα TGF-β1 και TGF-β2 cDNA, δείχνουν μία συνολική ομολογία 41%. Οι ώριμες και αμινο-τερματικές περιοχές, δείχνουν 71% και 31% ομολογία αντίστοιχα. Ανάλυση κηλίδος κατά Νorthern πιστοποίησε TGF--β2 αντίγραφα, 4.1 kb, 5.1, και 6.5 kb με τη χρησιμοποίηση mRNA από ορισμένες διαφορετικές πηγές. Ανάλυση πολυαδενυλιωμένου RNA από κύτταρα PC-3 που έχουν υποστεί κατεργασία με Tamoxifen, έδειξαν ότι αυτά τα κύτταρα περιέχουν μεγαλύτερες ποσότητες TGF-β1 αντιγράφων, από ό,τι ο TGF-β, αν και παράγουν περισσότερο TGF-β2 πρωτεϊνη, από ό,τι TGF-β1. Τούτο υποδηλώνει ότι υπάρχει ένα μετα-μεταγραφικό επίπεδο ρύθμισης για την παραγωγή αυτών των πρωτεϊνών. ω
GR880100660A 1987-10-06 1988-10-05 Κλωνισμος και εκφραση μετασχηματιστικου παραγοντα αναπτυξης β2. GR1000492B (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10675287A 1987-10-06 1987-10-06
US14826788A 1988-01-25 1988-01-25
US23406588A 1988-08-18 1988-08-18

Publications (1)

Publication Number Publication Date
GR1000492B true GR1000492B (el) 1992-07-30

Family

ID=27380183

Family Applications (1)

Application Number Title Priority Date Filing Date
GR880100660A GR1000492B (el) 1987-10-06 1988-10-05 Κλωνισμος και εκφραση μετασχηματιστικου παραγοντα αναπτυξης β2.

Country Status (22)

Country Link
JP (1) JPH02444A (el)
KR (1) KR970001236B1 (el)
CN (1) CN1035845A (el)
AT (1) AT398433B (el)
BE (1) BE1002693A5 (el)
CH (1) CH680002A5 (el)
DE (1) DE3833897C2 (el)
DK (1) DK556388A (el)
ES (1) ES2012556A6 (el)
FI (1) FI884546A (el)
FR (1) FR2621324B1 (el)
GB (1) GB2210620B8 (el)
GR (1) GR1000492B (el)
IE (1) IE60918B1 (el)
IL (1) IL87877A0 (el)
IT (1) IT1227957B (el)
MY (1) MY103779A (el)
NL (1) NL8802442A (el)
NO (1) NO884429L (el)
NZ (1) NZ226486A (el)
PT (1) PT88670B (el)
SE (1) SE8803528L (el)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5221620A (en) * 1987-10-06 1993-06-22 Oncogen Cloning and expression of transforming growth factor β2
US5445941A (en) * 1993-06-21 1995-08-29 Eli Lilly And Company Method for screening anti-osteoporosis agents
CN100389120C (zh) * 2005-04-12 2008-05-21 中国人民解放军第三军医大学野战外科研究所 封闭TGF－β1的反义寡核苷酸及其在制备治疗纤维化相关疾病药物中的用途

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0169016A2 (en) * 1984-07-16 1986-01-22 Celtrix Pharmaceuticals, Inc. Polypeptide cartilage-inducing factors found in bone
EP0200341A1 (en) * 1985-03-22 1986-11-05 Genentech, Inc. Nucleic acid encoding TGF-beta and its uses
EP0268561A2 (en) * 1986-11-17 1988-05-25 Sandoz Ag Production and use of a novel T-cell suppressor factor
WO1988005787A1 (en) * 1987-01-30 1988-08-11 Techne Corporation Transforming growth factor-beta

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4931548A (en) * 1987-01-30 1990-06-05 Techne Corporation Heterodimer form of transforming growth factor-beta
AU1539188A (en) * 1987-05-04 1988-11-10 Bristol-Myers Squibb Company TGF-B2 and novel compositions having anti-neoplastic activity

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0169016A2 (en) * 1984-07-16 1986-01-22 Celtrix Pharmaceuticals, Inc. Polypeptide cartilage-inducing factors found in bone
EP0200341A1 (en) * 1985-03-22 1986-11-05 Genentech, Inc. Nucleic acid encoding TGF-beta and its uses
EP0268561A2 (en) * 1986-11-17 1988-05-25 Sandoz Ag Production and use of a novel T-cell suppressor factor
WO1988005787A1 (en) * 1987-01-30 1988-08-11 Techne Corporation Transforming growth factor-beta

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
BIOCHEMISTRY, vol. 26, no. 9, 5th May 1987, pages 2406-2410, Washington, DC, US; T. IKEDA et al.: "Human transforming growth factor type beta2: Production by a prostatic adenocarcinoma cell line, purification, and initial characterization" *
CHEMICAL ABSTRACTS, vol. 109, no. 19, 7th November 1988, page 198, abstract no. 164768b, Columbus, Ohio, US; N.R. WEBB et al.: "Structural and sequence analysis of TGF-beta2 cDNA clones predicts two different precursor proteins produced by alternative mRNA splicing", & DNA 1988, 7(7), 493-7 (Cat. P,X) *
CHEMICAL ABSTRACTS, vol. 109, no. 5, 1st August 1988, page 159, abstract no. 32967f, Columbus, Ohio, US; L. MADISEN et al.: "Transforming growth factor-beta2: cDNA cloning and sequence analysis", & DNA 1988, 7(1), 1-8 *
CHEMICAL ABSTRACTS, vol. 111, no. 7, 14th August 1989, page 147, abstract no. 51117m, Columbus, Ohio, US; L. MADISEN et al.: "Expression and characterization of recombinant TGF-beta2 proteins produced in mammalian cells", & DNA 1989, 8(3), 205-12 *
CLAIMS SEARCHED: ALL *
J. BIOL. CHEM., vol. 262, no. 25, 5th September 1987, pages 12127-12131, The American Society for Biochemistry and Molecular Biology Inc., Baltimore, US; H. MARQUARDT et al.: "Complete amino acid sequence of human transforming growth factor type beta2" *
J. CELLULAR BIOCHEMISTRY SUPPLEMENT, vol. O, no. 12, part A, 1988, page 199, abstract D116; L. MADISEN et al.: "cDNA cloning and sequence analysis of tranforming growth factor-beta 2" *
NATURE, vol. 316, 22nd August 1985, pages 701-705, London, GB; R. DERYNCK et al.: "Human transforming growth factor-beta complementary DNA sequence and expression in normal and tranformed cells" *

Also Published As

Publication number Publication date
FI884546A0 (fi) 1988-10-03
GB2210620A (en) 1989-06-14
NO884429L (no) 1989-04-07
IE883018L (en) 1989-04-06
KR970001236B1 (ko) 1997-02-04
ES2012556A6 (es) 1990-04-01
DK556388D0 (da) 1988-10-05
DE3833897C2 (de) 1997-03-20
DK556388A (da) 1989-04-07
GB2210620B (en) 1992-07-08
GB8823448D0 (en) 1988-11-16
NO884429D0 (no) 1988-10-05
IT8822213A0 (it) 1988-10-06
CN1035845A (zh) 1989-09-27
FR2621324B1 (fr) 1994-02-18
PT88670B (pt) 1992-12-31
JPH02444A (ja) 1990-01-05
IE60918B1 (en) 1994-08-24
GB2210620B8 (en) 1995-07-17
ATA245488A (de) 1994-04-15
BE1002693A5 (fr) 1991-05-07
IT1227957B (it) 1991-05-20
IL87877A0 (en) 1989-03-31
KR890006814A (ko) 1989-06-16
FR2621324A1 (fr) 1989-04-07
SE8803528L (sv) 1989-04-07
CH680002A5 (el) 1992-05-29
MY103779A (en) 1993-09-30
DE3833897A1 (de) 1989-05-03
AT398433B (de) 1994-12-27
NL8802442A (nl) 1989-05-01
NZ226486A (en) 1991-08-27
SE8803528D0 (sv) 1988-10-05
FI884546A (fi) 1989-04-07

Similar Documents

Publication Publication Date Title
CA1269055A (en) Process for producing human epidermal growth factors and analogs thereof
NO882772L (no) Nye former koloni-stimulerende faktor-1.
DK0946732T3 (da) Fremgangsmåde til den rekombinante fremstilling af 1,3-propandiol
SE8603145D0 (sv) Forfarande for framstellning av interferon-beta
AR016537A1 (es) Una celula humana para la fabricacion de eritropoyetina por activacion genica endogena, un procedimiento para la fabricacion de la misma y una construccionde adn a ser usada en el mismo, un procedimiento para la fabricacion de epo humana, epo humana aislada obtenida por el mismo y un preparado farma
HRP20020767B1 (en) Production of recombinant blood clotting factors in human cell lines
DK164940C (da) Polypeptid med human-gamma-interferonaktivitet, genteknologisk fremgangsmaade til fremstilling deraf, hybrid-plasmider indeholdende en dna-sekvens, der koder for peptidet, vaertsorganismer indeholdende hybridplasmiderne, samt laegemidler, som indeholder polypeptidet
ES2062993T3 (es) Factor de crecimiento humano i semejante a la preproinsulina.
ATE334223T1 (de) Verfahren zur herstellung eines rekombinanten polypeptids unter hinzufügung eines hemmstoffes einer metallabhängigen protease oder chymotrypsins zum zellkulturmedium
ATE256739T1 (de) Hypothalamus-spezifische polypeptide
ES2173856T3 (es) Proceso para la preparacion de vectores geneticos para la expresion del factor de crecimiento nervioso en celulas eucariotas.
Gebhard et al. Complementary DNA and derived amino acid sequence of the precursor of one of the three protein components of the inter‐α‐trypsin inhibitor complex
GR1000492B (el) Κλωνισμος και εκφραση μετασχηματιστικου παραγοντα αναπτυξης β2.
GB2182665A (en) Dna clone of human skin fibroblast collagenase
Negro et al. Synthesis, purification, and characterization of human ciliary neuronotrophic factor from E. coli
Byrne et al. Human granulocyte-macrophage colony-stimulating factor purified from a Hodgkin's tumor cell line
ATE173762T1 (de) Klonierung der n-methylhydantoinase
Nishikawa et al. Efficient cleavage by α-thrombin of a recombinant fused protein which contains insulin-like growth factor I
ES2127192T3 (es) Polipeptidos especificos del epididimo y su aplicacion.
DE69738135D1 (de) Modifizierte methylotrophe p. pastoris hefe, welche menschliches wachstumshormon sekretiert
AR014731A1 (es) Fragmento aislado de acidos nucleicos que codifica toda o una porcion sustancial de una proteina inhibidora de la apoptosis en plantas y semillas,gen quimerico, celula huesped transformada, polipeptido, metodo para alterar el nivel de expresion de dicha proteina en una celula huesped, metodos para
AR025482A1 (es) Secuencia de adn que codifica una proteina capaz de ligarse a traf; vector que comprende dicha secuencia; celulas huespedes transformadas con dicho vector;proteinas iren codificadas por dicha secuencia; metodo para producir dicha proteina; anticuerpos especificos para dichas proteinas; metodo para m
JPH03219880A (ja) 細菌コラゲナーゼ遺伝子
ES2039390T3 (es) Un procedimiento para producir un factor de diferenciacion de celulas b humanas.
Rock et al. Overexpression and structure-function analysis of a bioengineered IL-2/IL-6 chimeric lymphokine