GB853563A - Lysine racemase and the preparation of dl-lysine - Google Patents
Lysine racemase and the preparation of dl-lysineInfo
- Publication number
- GB853563A GB853563A GB20994/58A GB2099458A GB853563A GB 853563 A GB853563 A GB 853563A GB 20994/58 A GB20994/58 A GB 20994/58A GB 2099458 A GB2099458 A GB 2099458A GB 853563 A GB853563 A GB 853563A
- Authority
- GB
- United Kingdom
- Prior art keywords
- lysine
- racemase
- americanus
- aqueous
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Lysine racemase, an enzyme capable of racemising D-or L-lysine to DL-lysine, is produced by growing a lysine-racemase-producing strain of Proteus vulganis, P. ammoniae, P. mirabilis, P. americanus, P. para-americanus, P. rettgeri, P. spingidis, P. inconstans, Escherichia anindolica and E. freundii in an aqueous nutrient medium containing sources of carbohydrate, organic nitrogen and inorganic nitrogen at an initial pH between 6,0 and 8,0. The temperature of cultivation is 20 DEG to 40 DEG C. and the process is preferably submerged and the duration may be one day. A suitable medium contains cornsteep liquor, ammonium hydrogen phosphate, crude beet molasses, glycerol and magnesium sulphate. For the production of DL-lysine from D-lysine or L-lysine, the lysine racemase is contacted at pH 7,0 and 9,0 and temperature of 20. to 40 DEG C. with the lysine. After acidification the DL-lysine may be absorbed on a sulphonic acid resin eluted with aqueous alkali and crystallised by known methods. The lysine racemete may be used in the form of (a) the whole broth adjusted to pH 7,0 to 9,0 and preferably covered with toluene to prevent contamination (b) bacterial cells removed from the broth, washed with water and suspended in an aqueous medium at pH 7,0 to 9,0 and (c) the cell free product.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US853563XA | 1957-07-30 | 1957-07-30 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB853563A true GB853563A (en) | 1960-11-09 |
Family
ID=22191068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB20994/58A Expired GB853563A (en) | 1957-07-30 | 1958-07-01 | Lysine racemase and the preparation of dl-lysine |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB853563A (en) |
-
1958
- 1958-07-01 GB GB20994/58A patent/GB853563A/en not_active Expired
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