GB808300A - Improvements in a soluble enzyme preparation and process of recovery of the same - Google Patents
Improvements in a soluble enzyme preparation and process of recovery of the sameInfo
- Publication number
- GB808300A GB808300A GB37650/56A GB3765056A GB808300A GB 808300 A GB808300 A GB 808300A GB 37650/56 A GB37650/56 A GB 37650/56A GB 3765056 A GB3765056 A GB 3765056A GB 808300 A GB808300 A GB 808300A
- Authority
- GB
- United Kingdom
- Prior art keywords
- weight
- sodium
- per cent
- acid
- papain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A process of recovering from crude commercial papain, however previously processed, an enzyme of enhanced proteolytic activity, colour, solubility, odour and keeping qualities comprises extracting, preferably at 60-90 DEG F., the crude papain with an aqueous solution containing a compound such as sulphurous acid, sodium bisulphite or potassium metabisulphite, which provides 1 to 4 per cent by weight of available SO2, and 0.5 to 3 per cent by weight of a chelating agent, the weight ratio of crude papain to extracting solution preferably being between 0.3 and 0.5, the pH being between 4.5 and 7.0, preferably 5.0 and 6.0, then centrifuging or filtering with the aid of diatomaceous earth and finally spray-drying the clarified extract at a temperature which may be 250-600 DEG F. Specified chelating agents are ethylenediamine tetra-acetic acid and its sodium salt, phytic acid and its sodium and calcium salts.ALSO:A stable, soluble, highly active, powdered proteolytic enzyme composition comprises an extract of commercial crude papain with an aqueous solution containing 0.5 to 3 per cent by weight of a chelating agent such as ethylenediamine tetra-acetic acid and its sodium salt, phytic acid and its sodium and calcium salts, or citric acid and its sodium salt and 1 to 4 per cent by weight of available sulphur dioxide supplied from a compound such as sulphurous acid, sodium bisulphite or potassium metabisulphite, which extract has been clarified and spray dried.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US808300XA | 1955-12-28 | 1955-12-28 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB808300A true GB808300A (en) | 1959-02-04 |
Family
ID=22159873
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB37650/56A Expired GB808300A (en) | 1955-12-28 | 1956-12-10 | Improvements in a soluble enzyme preparation and process of recovery of the same |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB808300A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2122488A (en) * | 1982-05-29 | 1984-01-18 | Reiko Kosaka | Compositions containing papain and citric acid |
| FR2754269A1 (en) * | 1996-10-04 | 1998-04-10 | Rhodia Chimie Sa | Drying of heat-sensitive substances, especially enzymes |
-
1956
- 1956-12-10 GB GB37650/56A patent/GB808300A/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2122488A (en) * | 1982-05-29 | 1984-01-18 | Reiko Kosaka | Compositions containing papain and citric acid |
| FR2754269A1 (en) * | 1996-10-04 | 1998-04-10 | Rhodia Chimie Sa | Drying of heat-sensitive substances, especially enzymes |
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