GB761278A - Improved method of collecting micro-organisms growing on a solid matrix - Google Patents
Improved method of collecting micro-organisms growing on a solid matrixInfo
- Publication number
- GB761278A GB761278A GB13566/54A GB1356654A GB761278A GB 761278 A GB761278 A GB 761278A GB 13566/54 A GB13566/54 A GB 13566/54A GB 1356654 A GB1356654 A GB 1356654A GB 761278 A GB761278 A GB 761278A
- Authority
- GB
- United Kingdom
- Prior art keywords
- matrix
- penicillium
- culture
- bacterium
- micro
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Micro-organisms growing on a solid matrix may be collected by subjecting the culture to sonic oscillations in such manner that the parts of the micro-organisms projecting from the surface of the matrix are removed therefrom without admixture of the constituents of the matrix. A liquid layer, e.g. water, is preferably applied to the matrix prior to subjecting the culture to the sonic oscillations, thus producing a dispersion. A surface-active agent may be added to the water layer to increase the degree of dispersion obtained. The preferred frequency range is from 20,000 to 30,000 cycles/second. The process is applicable to cultures of Penicillium chrysogenum, Penicillium notatum, Penicillium olivaceum, Aspergillus niger, Rhizopus nigricans, Streptomyces griseus, Bacterium coli, Bacterium subtilis and Bacterium mesentericus. In an example a culture of Penicillium chrysogentum on an agar-agar sheet is coated with water and subjected to oscillations of frequency 22,000 c/s in a magnetostriction apparatus, to give a dark-green suspension.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| NL761278X | 1953-05-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB761278A true GB761278A (en) | 1956-11-14 |
Family
ID=19827241
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB13566/54A Expired GB761278A (en) | 1953-05-13 | 1954-05-10 | Improved method of collecting micro-organisms growing on a solid matrix |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB761278A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3300390A (en) * | 1964-03-23 | 1967-01-24 | Melpar Inc | Process for harvest of spores from aerial microbial structures |
| EP0032322A2 (en) * | 1980-01-07 | 1981-07-22 | Kozak, Peter, P., Jr. | Method for obtaining mold spore material |
| US4294930A (en) * | 1979-08-22 | 1981-10-13 | Miles Laboratories, Inc. | Process for improving the recovery of microbial cell biomass |
| FR2537156A1 (en) * | 1982-12-01 | 1984-06-08 | Inst Nat Sante Rech Med | Apparatus for mechanical detachment of cells |
| WO1985001514A1 (en) * | 1983-09-26 | 1985-04-11 | University Of Iowa Research Foundation | A method of and apparatus for harvesting mammalian cells |
-
1954
- 1954-05-10 GB GB13566/54A patent/GB761278A/en not_active Expired
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3300390A (en) * | 1964-03-23 | 1967-01-24 | Melpar Inc | Process for harvest of spores from aerial microbial structures |
| US4294930A (en) * | 1979-08-22 | 1981-10-13 | Miles Laboratories, Inc. | Process for improving the recovery of microbial cell biomass |
| EP0032322A2 (en) * | 1980-01-07 | 1981-07-22 | Kozak, Peter, P., Jr. | Method for obtaining mold spore material |
| EP0032322A3 (en) * | 1980-01-07 | 1981-08-26 | Kozak, Peter, P., Jr. | Method for obtaining mold spore material |
| FR2537156A1 (en) * | 1982-12-01 | 1984-06-08 | Inst Nat Sante Rech Med | Apparatus for mechanical detachment of cells |
| WO1985001514A1 (en) * | 1983-09-26 | 1985-04-11 | University Of Iowa Research Foundation | A method of and apparatus for harvesting mammalian cells |
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