GB730444A - Improvements in or relating to nucleotides - Google Patents
Improvements in or relating to nucleotidesInfo
- Publication number
- GB730444A GB730444A GB21309/53A GB2130953A GB730444A GB 730444 A GB730444 A GB 730444A GB 21309/53 A GB21309/53 A GB 21309/53A GB 2130953 A GB2130953 A GB 2130953A GB 730444 A GB730444 A GB 730444A
- Authority
- GB
- United Kingdom
- Prior art keywords
- salt
- nucleotide
- splitting
- components
- alkaloid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Diphosphopyridine-nucleotide (DPN) is purified by forming a salt therewith and a cinchona alkaloid and then splitting the salt into its components. The salt may be formed by mixing solutions of the components or by double decomposition, e.g. from the barium salt of the nucleotide and quinine sulphate. The splitting of the salt is achieved by passing a solution thereof through an ion-exchange resin in the acetate form, whereby the alkaloid acetate passes through and the nucleotide is adsorbed. The latter may be eluted with a strong acid and precipitated with an organic solvent. An example is given of the process.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE730444X | 1952-08-04 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB730444A true GB730444A (en) | 1955-05-25 |
Family
ID=6636672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB21309/53A Expired GB730444A (en) | 1952-08-04 | 1953-07-31 | Improvements in or relating to nucleotides |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB730444A (en) |
-
1953
- 1953-07-31 GB GB21309/53A patent/GB730444A/en not_active Expired
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