GB2153356B - Nucleoside phosphoramidites and oligonucleotides produced therefrom - Google Patents

Nucleoside phosphoramidites and oligonucleotides produced therefrom

Info

Publication number
GB2153356B
GB2153356B GB08432205A GB8432205A GB2153356B GB 2153356 B GB2153356 B GB 2153356B GB 08432205 A GB08432205 A GB 08432205A GB 8432205 A GB8432205 A GB 8432205A GB 2153356 B GB2153356 B GB 2153356B
Authority
GB
United Kingdom
Prior art keywords
produced therefrom
nucleoside phosphoramidites
oligonucleotides produced
oligonucleotides
phosphoramidites
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB08432205A
Other languages
English (en)
Other versions
GB8432205D0 (en
GB2153356A (en
Inventor
Lloyd M Smith
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology
Original Assignee
California Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Institute of Technology filed Critical California Institute of Technology
Publication of GB8432205D0 publication Critical patent/GB8432205D0/en
Priority to GB8517170A priority Critical patent/GB2162571B/en
Publication of GB2153356A publication Critical patent/GB2153356A/en
Application granted granted Critical
Publication of GB2153356B publication Critical patent/GB2153356B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
GB08432205A 1983-11-15 1984-12-20 Nucleoside phosphoramidites and oligonucleotides produced therefrom Expired GB2153356B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
GB8517170A GB2162571B (en) 1983-11-15 1985-07-05 Glazing gasket

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US56501083A 1983-12-20 1983-12-20

Publications (3)

Publication Number Publication Date
GB8432205D0 GB8432205D0 (en) 1985-01-30
GB2153356A GB2153356A (en) 1985-08-21
GB2153356B true GB2153356B (en) 1987-07-22

Family

ID=24256835

Family Applications (1)

Application Number Title Priority Date Filing Date
GB08432205A Expired GB2153356B (en) 1983-11-15 1984-12-20 Nucleoside phosphoramidites and oligonucleotides produced therefrom

Country Status (6)

Country Link
JP (3) JPS60197698A (enExample)
CA (1) CA1244786A (enExample)
DE (1) DE3446635C2 (enExample)
FR (1) FR2556726B1 (enExample)
GB (1) GB2153356B (enExample)
SE (1) SE466208B (enExample)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US5118800A (en) * 1983-12-20 1992-06-02 California Institute Of Technology Oligonucleotides possessing a primary amino group in the terminal nucleotide
US4849513A (en) * 1983-12-20 1989-07-18 California Institute Of Technology Deoxyribonucleoside phosphoramidites in which an aliphatic amino group is attached to the sugar ring and their use for the preparation of oligonucleotides containing aliphatic amino groups
US5015733A (en) * 1983-12-20 1991-05-14 California Institute Of Technology Nucleosides possessing blocked aliphatic amino groups
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
USRE43096E1 (en) 1984-01-16 2012-01-10 California Institute Of Technology Tagged extendable primers and extension products
GB8509880D0 (en) * 1985-04-17 1985-05-22 Ici Plc Testing device
US4762779A (en) * 1985-06-13 1988-08-09 Amgen Inc. Compositions and methods for functionalizing nucleic acids
US4757141A (en) * 1985-08-26 1988-07-12 Applied Biosystems, Incorporated Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof
US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
DE3642939A1 (de) * 1986-06-03 1987-12-10 Europ Lab Molekularbiolog Verfahren zur dna-markierung
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5242796A (en) * 1986-07-02 1993-09-07 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US4904582A (en) * 1987-06-11 1990-02-27 Synthetic Genetics Novel amphiphilic nucleic acid conjugates
US4833332A (en) * 1987-06-12 1989-05-23 E. I. Du Pont De Nemours And Company Scanning fluorescent detection system
US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US4997928A (en) * 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
US5262536A (en) * 1988-09-15 1993-11-16 E. I. Du Pont De Nemours And Company Reagents for the preparation of 5'-tagged oligonucleotides
GB8822228D0 (en) * 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
AU9158391A (en) * 1990-12-11 1992-07-08 Abbott Laboratories Methods for labelling oligonucleotides
US5643722A (en) * 1994-05-11 1997-07-01 Trustees Of Boston University Methods for the detection and isolation of proteins
US6020481A (en) 1996-04-01 2000-02-01 The Perkin-Elmer Corporation Asymmetric benzoxanthene dyes
SE522077C2 (sv) 1997-09-05 2004-01-13 Lightup Technologies Ab Förfarande att välja sekvens hos sond för nukleinsyrahybridisering
DE19815864A1 (de) 1998-04-08 1999-10-14 Europ Lab Molekularbiolog 5'-modifizierte Nukleotide und ihre Anwendung in der Molekularbiologie und Medizin
CA2370478A1 (en) 1999-03-24 2000-09-28 Serge L. Beaucage N-acylphosphoramidites and their use in oligonucleotide synthesis
US7355037B2 (en) 2001-12-03 2008-04-08 The United States Of America As Represented By The Department Of Health And Human Services Thermolabile hydroxyl protecting groups and methods of use
WO2006065751A2 (en) 2004-12-13 2006-06-22 Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Cpg oligonucleotide prodrugs, compositions thereof and associated therapeutic methods

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3284440A (en) * 1964-06-12 1966-11-08 Merck & Co Inc Phosphate esters of cytosine arabinoide and process for preparing same
US3847898A (en) * 1969-05-27 1974-11-12 Upjohn Co N4-trihaloethoxy carbonyl arabino-furanosyl cytosine 5'-esters
DE2924332A1 (de) * 1978-06-22 1980-01-03 Miles Lab Flavin-adenin-dinukleotid-markiertes konjugat und dessen verwendung in spezifischen bindungsversuchen
IL57570A (en) * 1978-06-22 1982-07-30 Miles Lab Method and reagent for specific binding assay with a prosthetic group as a label component
US4415732A (en) * 1981-03-27 1983-11-15 University Patents, Inc. Phosphoramidite compounds and processes
FR2519005B1 (fr) * 1981-12-29 1985-10-25 Pasteur Institut Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn

Also Published As

Publication number Publication date
JPH0157119B2 (enExample) 1989-12-04
CA1244786A (en) 1988-11-15
SE466208B (sv) 1992-01-13
FR2556726B1 (fr) 1987-02-20
GB8432205D0 (en) 1985-01-30
SE8406484L (sv) 1985-06-21
JPS60197698A (ja) 1985-10-07
JPH06145192A (ja) 1994-05-24
SE8406484D0 (sv) 1984-12-19
JPH0678353B2 (ja) 1994-10-05
JPH0460600B2 (enExample) 1992-09-28
GB2153356A (en) 1985-08-21
FR2556726A1 (fr) 1985-06-21
DE3446635C2 (de) 1997-08-07
JPH01250393A (ja) 1989-10-05
DE3446635A1 (de) 1985-06-27

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Legal Events

Date Code Title Description
PE20 Patent expired after termination of 20 years

Effective date: 20041219