GB2123428A - Method for production of predetermined polyribonucleotides - Google Patents

Method for production of predetermined polyribonucleotides

Info

Publication number
GB2123428A
GB2123428A GB08324625A GB8324625A GB2123428A GB 2123428 A GB2123428 A GB 2123428A GB 08324625 A GB08324625 A GB 08324625A GB 8324625 A GB8324625 A GB 8324625A GB 2123428 A GB2123428 A GB 2123428A
Authority
GB
United Kingdom
Prior art keywords
ribonucleotide
recipient
methods
donor
predetermined
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB08324625A
Other versions
GB8324625D0 (en
GB2123428B (en
Inventor
Hiroaki Shizuya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INNOVAX LAB Ltd
Innovax Laboratories Ltd
Original Assignee
INNOVAX LAB Ltd
Innovax Laboratories Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INNOVAX LAB Ltd, Innovax Laboratories Ltd filed Critical INNOVAX LAB Ltd
Publication of GB8324625D0 publication Critical patent/GB8324625D0/en
Publication of GB2123428A publication Critical patent/GB2123428A/en
Application granted granted Critical
Publication of GB2123428B publication Critical patent/GB2123428B/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)

Abstract

Methods of production of predetermined polyribonucleotide. These methods may then be used to synthesize RNA coding for a desired protein. The methods described include: (a) adding a single nucleotide on to the 3' end of a recipient ribonucleotide of predetermined composition by reacting the compound of the form AppNp with the recipient ribonucleotide in the presence of T4 RNA ligase and isolating the extended ribonucleotide where N is a ribonucleotide selected from the group adenine, guanine, cytosine, uracil and inosine. (b) adding a ribonucleotide of at least two bases by phosphorylating a donor ribonucleotide of at least two bases at the terminal 5' and 3' ends, then mixing the phosphorylated donor ribonucleotide with a recipient ribonucleotide in the presence of T4 RNA ligase to add the donor onto the 3' end of the recipient ribonucleotide where the recipient nucleotide is selected from the group consisting of a dinucleotide phosphorylated in the free 5' position and a nucleotide longer than 2 base pairs with either a hydroxyl or a phosphate group on the 5' terminus and a hydroxylated 3' end. (c) synthesizing ribonucleic acid of a predetermined composition by sequentially building polynucleotides using the above methods.
GB08324625A 1982-02-01 1983-01-25 Method for production of predetermined polyribonucleotides Expired GB2123428B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34478982A 1982-02-01 1982-02-01
PCT/US1983/000115 WO1983002626A1 (en) 1982-02-01 1983-01-25 Method for production of predetermined polyribonucleotides

Publications (3)

Publication Number Publication Date
GB8324625D0 GB8324625D0 (en) 1983-10-19
GB2123428A true GB2123428A (en) 1984-02-01
GB2123428B GB2123428B (en) 1986-02-05

Family

ID=23352042

Family Applications (1)

Application Number Title Priority Date Filing Date
GB08324625A Expired GB2123428B (en) 1982-02-01 1983-01-25 Method for production of predetermined polyribonucleotides

Country Status (7)

Country Link
EP (1) EP0100353A1 (en)
JP (1) JPS59500081A (en)
AU (1) AU1334983A (en)
DE (1) DE3328937T1 (en)
FR (1) FR2522682A1 (en)
GB (1) GB2123428B (en)
WO (1) WO1983002626A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1295830B1 (en) * 1997-10-28 1999-05-28 Angeletti P Ist Richerche Bio PROCEDURE FOR THE SYNTHESIS OF POLYNUCLEOTIDES HAVING TOTALLY OR PARTIALLY RANDOM NUCLEOTIDE SEQUENCES
US6479262B1 (en) * 2000-05-16 2002-11-12 Hercules, Incorporated Solid phase enzymatic assembly of polynucleotides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS56144095A (en) * 1980-04-10 1981-11-10 Mitsubishi Chem Ind Ltd Preparation of oligoribonucleotide

Also Published As

Publication number Publication date
WO1983002626A1 (en) 1983-08-04
EP0100353A1 (en) 1984-02-15
JPS59500081A (en) 1984-01-19
AU1334983A (en) 1983-08-12
GB8324625D0 (en) 1983-10-19
GB2123428B (en) 1986-02-05
DE3328937T1 (en) 1984-10-18
FR2522682A1 (en) 1983-09-09

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee