JPS56144095A - Preparation of oligoribonucleotide - Google Patents

Preparation of oligoribonucleotide

Info

Publication number
JPS56144095A
JPS56144095A JP4709680A JP4709680A JPS56144095A JP S56144095 A JPS56144095 A JP S56144095A JP 4709680 A JP4709680 A JP 4709680A JP 4709680 A JP4709680 A JP 4709680A JP S56144095 A JPS56144095 A JP S56144095A
Authority
JP
Japan
Prior art keywords
diphosphate
diribonucleotide
oligoribonucleotide
preparation
ribonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP4709680A
Other languages
Japanese (ja)
Other versions
JPH0226958B2 (en
Inventor
Isao Uchida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Mitsubishi Kasei Corp
Original Assignee
Mitsubishi Kasei Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsubishi Kasei Corp filed Critical Mitsubishi Kasei Corp
Priority to JP4709680A priority Critical patent/JPS56144095A/en
Publication of JPS56144095A publication Critical patent/JPS56144095A/en
Publication of JPH0226958B2 publication Critical patent/JPH0226958B2/ja
Granted legal-status Critical Current

Links

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  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PURPOSE: To make the preparation of oligoribonucleotide easy, by using RNA ligase as a catalyst in reacting a 5'-phosphoalkyl ester of diribonucleotide 5',3'-diphosphate with ribonucleotide 5',3-diphosphate.
CONSTITUTION: Diribonucleotide 5',3'-diphosphate with its hydroxyl group blocked, e.g., 5'-phosphoalkyl ester, 5'-phosphoanililate, etc. of diribonucleotide 5',3'-diphosphate, is reacted with ribonucleotide 5',3'-diphosphate in a buffer solution containing RNA ligase, adenosinetriphosphosphate, MgCl2, bovine serum albumin, etc. The reaction product is obtained by column chromatography.
COPYRIGHT: (C)1981,JPO&Japio
JP4709680A 1980-04-10 1980-04-10 Preparation of oligoribonucleotide Granted JPS56144095A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP4709680A JPS56144095A (en) 1980-04-10 1980-04-10 Preparation of oligoribonucleotide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP4709680A JPS56144095A (en) 1980-04-10 1980-04-10 Preparation of oligoribonucleotide

Publications (2)

Publication Number Publication Date
JPS56144095A true JPS56144095A (en) 1981-11-10
JPH0226958B2 JPH0226958B2 (en) 1990-06-13

Family

ID=12765648

Family Applications (1)

Application Number Title Priority Date Filing Date
JP4709680A Granted JPS56144095A (en) 1980-04-10 1980-04-10 Preparation of oligoribonucleotide

Country Status (1)

Country Link
JP (1) JPS56144095A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2522682A1 (en) * 1982-02-01 1983-09-09 Innovax Lab Ltd PROCESS FOR PRODUCING PREDETERMINED POLYRIBONUCLEOTIDES

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH09159926A (en) * 1995-12-14 1997-06-20 Nec Corp Wide-angle periscopic device

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
BIOCHEMISTRY=1978 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2522682A1 (en) * 1982-02-01 1983-09-09 Innovax Lab Ltd PROCESS FOR PRODUCING PREDETERMINED POLYRIBONUCLEOTIDES

Also Published As

Publication number Publication date
JPH0226958B2 (en) 1990-06-13

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