GB2007244A - Purifying Polyribosyl Ribitol Phosphate (PRP) and Vaccines containing Immunologically Active PRP - Google Patents

Purifying Polyribosyl Ribitol Phosphate (PRP) and Vaccines containing Immunologically Active PRP

Info

Publication number
GB2007244A
GB2007244A GB7842230A GB7842230A GB2007244A GB 2007244 A GB2007244 A GB 2007244A GB 7842230 A GB7842230 A GB 7842230A GB 7842230 A GB7842230 A GB 7842230A GB 2007244 A GB2007244 A GB 2007244A
Authority
GB
United Kingdom
Prior art keywords
prp
immunologically active
vaccines containing
antibody
purifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
GB7842230A
Other versions
GB2007244B (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth Holdings LLC
Original Assignee
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US05/846,466 external-priority patent/US4196192A/en
Priority claimed from US05/846,488 external-priority patent/US4220717A/en
Application filed by American Cyanamid Co filed Critical American Cyanamid Co
Publication of GB2007244A publication Critical patent/GB2007244A/en
Application granted granted Critical
Publication of GB2007244B publication Critical patent/GB2007244B/en
Expired legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/099—Bordetella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/102—Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/70—Multivalent vaccine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

The invention provides methods for the purification of polyribosyl ribitol phosphate (PRP) from Haemophilus influenzae type b cultures, as well as novel forms of immunologically active PRP obtainable by such methods and combined vaccines containing immunologically active PRP and Bordetella pertussis antigens. The purification methods involve removing contaminants to a low level by treating the PRP in the form of a hexadecyltrimethyl ammonium bromide complex with a calcium phosphate adsorbent (hydroxylapatite) in a phosphate buffer, and may be practiced either as a batch process or as a column process. The purified PRP thus obtainable is immunologically active and may be used with B. pertussis antigens to form a combined vaccine which elicits anti-PRP antibody and antipercussis antibody formations in young animals. This sera with anti-PRP antibody exhibits a strong bactericidal activity.
GB7842230A 1977-10-28 1978-10-27 Purifying polyribosyl ribitol phosphate (prp) and vaccines containing immunoligically active prp Expired GB2007244B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US05/846,466 US4196192A (en) 1977-10-28 1977-10-28 Combined Haemophilus influenzae type b and pertussis vaccine
US05/846,488 US4220717A (en) 1977-12-22 1977-12-22 Isolation and purification of polyribosyl ribitol phosphate from Haemophilus influenzae type b.

Publications (2)

Publication Number Publication Date
GB2007244A true GB2007244A (en) 1979-05-16
GB2007244B GB2007244B (en) 1982-03-31

Family

ID=27126641

Family Applications (1)

Application Number Title Priority Date Filing Date
GB7842230A Expired GB2007244B (en) 1977-10-28 1978-10-27 Purifying polyribosyl ribitol phosphate (prp) and vaccines containing immunoligically active prp

Country Status (25)

Country Link
JP (1) JPS5480408A (en)
AR (1) AR218932A1 (en)
AU (1) AU520902B2 (en)
BE (1) BE871586A (en)
CA (1) CA1117866A (en)
CH (1) CH649781A5 (en)
DD (1) DD140701A5 (en)
DE (1) DE2845745A1 (en)
DK (1) DK154327C (en)
ES (1) ES474611A1 (en)
FI (1) FI63674C (en)
FR (1) FR2407000B1 (en)
GB (1) GB2007244B (en)
GR (1) GR73991B (en)
HU (1) HU178166B (en)
IE (1) IE47474B1 (en)
IL (1) IL55433A (en)
IT (1) IT1107570B (en)
NL (1) NL7810753A (en)
NO (1) NO149611C (en)
NZ (1) NZ188211A (en)
PH (1) PH15882A (en)
PL (1) PL210545A1 (en)
SE (1) SE430753B (en)
YU (1) YU250878A (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE889979A (en) * 1981-08-14 1982-02-15 Smith Kline Rit PROCESS FOR THE PREPARATION OF PURIFIED ANTIGENIC CAPSULAR BACTERIAL POLYSACCHARIDES, PRODUCTS OBTAINED AND THEIR USE
CA1209036A (en) * 1982-08-20 1986-08-05 Joseph S.C. Kuo Combined haemophilus influenzae and diphtheria, pertussis, tetanus vaccine
US4744982A (en) * 1982-08-24 1988-05-17 Hunter Kenneth W Human monoclonal antibody reactive with polyribosylribitol phosphate
ATE128628T1 (en) * 1990-08-13 1995-10-15 American Cyanamid Co FIBER HEMAGGLUTININ FROM BORDETELLA PERTUSSIS AS A CARRIER FOR CONJUGATE VACCINE.
HU9400426D0 (en) * 1991-08-16 1994-05-30 Merck & Co Inc Process for converting lipid-containing bacterial capsular polysaccharide into lipid-free polysaccharide

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3395219A (en) * 1964-12-11 1968-07-30 Merck & Co Inc Process for production of pertussis antigen
IL24946A (en) * 1965-01-19 1969-05-28 Merck & Co Inc Process for preparing b. pertussis antigens
US3465078A (en) * 1965-10-21 1969-09-02 Sydney Z Spiesel Method of recovering antigens from bordetella pertussis cells
NL174267B (en) * 1969-05-20 Roussel Uclaf IMPROVEMENT OF THE PROCESS FOR THE PREPARATION OF A SOMATIC ANTIGEN ACCORDING TO DUTCH PATENT 169754 AND PROCESS FOR PREPARING A PHARMACEUTICAL PREPARATION.
US3636192A (en) * 1970-01-13 1972-01-18 Us Army Meningococcal polysaccharide vaccines
FR2253499B1 (en) * 1973-12-10 1977-11-04 Fabre Sa Pierre
DE2461439C3 (en) * 1974-12-24 1980-03-20 Behringwerke Ag, 3550 Marburg Process for the preparation of a protective antigen from Bordetella pertussis and agent containing the same
US3978209A (en) * 1975-03-24 1976-08-31 Merck & Co., Inc. Endotoxin free meningococcus polysaccharides

Also Published As

Publication number Publication date
JPS6231694B2 (en) 1987-07-09
DK154327C (en) 1989-04-03
SE430753B (en) 1983-12-12
ES474611A1 (en) 1980-01-16
DD140701A5 (en) 1980-03-26
FI63674B (en) 1983-04-29
PL210545A1 (en) 1980-02-25
NZ188211A (en) 1984-09-28
BE871586A (en) 1979-04-27
NL7810753A (en) 1979-05-02
CH649781A5 (en) 1985-06-14
GR73991B (en) 1984-06-06
IE47474B1 (en) 1984-03-21
AU4108178A (en) 1980-05-01
DE2845745C2 (en) 1987-10-01
DK154327B (en) 1988-11-07
DE2845745A1 (en) 1979-05-10
IE782146L (en) 1979-04-28
SE7811192L (en) 1979-04-29
FI63674C (en) 1983-08-10
IT7851621A0 (en) 1978-10-24
FI783269A (en) 1979-04-29
PH15882A (en) 1983-04-13
DK479578A (en) 1979-04-29
NO149611C (en) 1984-05-23
FR2407000B1 (en) 1983-01-21
AU520902B2 (en) 1982-03-04
HU178166B (en) 1982-03-28
NO149611B (en) 1984-02-13
JPS5480408A (en) 1979-06-27
IT1107570B (en) 1985-11-25
NO783635L (en) 1979-05-02
IL55433A (en) 1982-09-30
AR218932A1 (en) 1980-07-15
CA1117866A (en) 1982-02-09
YU250878A (en) 1983-12-31
GB2007244B (en) 1982-03-31
FR2407000A1 (en) 1979-05-25
IL55433A0 (en) 1978-10-31

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee