GB1570698A - Method of generating a ph-gradient especially for use in electrophoresis - Google Patents
Method of generating a ph-gradient especially for use in electrophoresis Download PDFInfo
- Publication number
- GB1570698A GB1570698A GB51757/76A GB5175776A GB1570698A GB 1570698 A GB1570698 A GB 1570698A GB 51757/76 A GB51757/76 A GB 51757/76A GB 5175776 A GB5175776 A GB 5175776A GB 1570698 A GB1570698 A GB 1570698A
- Authority
- GB
- United Kingdom
- Prior art keywords
- gradient
- matrix
- groups
- immobilized
- generating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000000034 method Methods 0.000 title claims description 37
- 238000001962 electrophoresis Methods 0.000 title description 6
- 238000000926 separation method Methods 0.000 claims description 36
- 239000011159 matrix material Substances 0.000 claims description 27
- 239000000872 buffer Substances 0.000 claims description 10
- 239000002609 medium Substances 0.000 claims description 10
- 230000000087 stabilizing effect Effects 0.000 claims description 10
- 239000012736 aqueous medium Substances 0.000 claims description 4
- 229910052799 carbon Inorganic materials 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 229910052717 sulfur Inorganic materials 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 125000000524 functional group Chemical group 0.000 claims description 2
- 238000000760 immunoelectrophoresis Methods 0.000 claims description 2
- 238000007693 zone electrophoresis Methods 0.000 claims description 2
- 239000000959 ampholyte mixture Substances 0.000 description 10
- 239000000499 gel Substances 0.000 description 7
- -1 sulphonic acid- Chemical class 0.000 description 7
- 230000003139 buffering effect Effects 0.000 description 5
- 239000000178 monomer Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 4
- 239000002253 acid Chemical class 0.000 description 4
- 230000005684 electric field Effects 0.000 description 4
- 229920000642 polymer Polymers 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000037230 mobility Effects 0.000 description 3
- 229920002401 polyacrylamide Polymers 0.000 description 3
- 238000006116 polymerization reaction Methods 0.000 description 3
- 230000032258 transport Effects 0.000 description 3
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- VVJKKWFAADXIJK-UHFFFAOYSA-N Allylamine Chemical compound NCC=C VVJKKWFAADXIJK-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000003431 cross linking reagent Substances 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 2
- 239000001124 (E)-prop-1-ene-1,2,3-tricarboxylic acid Substances 0.000 description 1
- OSSNTDFYBPYIEC-UHFFFAOYSA-N 1-ethenylimidazole Chemical compound C=CN1C=CN=C1 OSSNTDFYBPYIEC-UHFFFAOYSA-N 0.000 description 1
- SMZOUWXMTYCWNB-UHFFFAOYSA-N 2-(2-methoxy-5-methylphenyl)ethanamine Chemical compound COC1=CC=C(C)C=C1CCN SMZOUWXMTYCWNB-UHFFFAOYSA-N 0.000 description 1
- JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N 2-Propenoic acid Natural products OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- KGIGUEBEKRSTEW-UHFFFAOYSA-N 2-vinylpyridine Chemical compound C=CC1=CC=CC=N1 KGIGUEBEKRSTEW-UHFFFAOYSA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 101500025736 Drosophila melanogaster CAP-1 Proteins 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 229940091181 aconitic acid Drugs 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- 125000004103 aminoalkyl group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical class OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000006172 buffering agent Substances 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical class OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- ANTSCNMPPGJYLG-UHFFFAOYSA-N chlordiazepoxide Chemical compound O=N=1CC(NC)=NC2=CC=C(Cl)C=C2C=1C1=CC=CC=C1 ANTSCNMPPGJYLG-UHFFFAOYSA-N 0.000 description 1
- GTZCVFVGUGFEME-IWQZZHSRSA-N cis-aconitic acid Chemical compound OC(=O)C\C(C(O)=O)=C\C(O)=O GTZCVFVGUGFEME-IWQZZHSRSA-N 0.000 description 1
- 238000010924 continuous production Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000003411 electrode reaction Methods 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 230000037427 ion transport Effects 0.000 description 1
- 230000005865 ionizing radiation Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 description 1
- DYUWTXWIYMHBQS-UHFFFAOYSA-N n-prop-2-enylprop-2-en-1-amine Chemical compound C=CCNCC=C DYUWTXWIYMHBQS-UHFFFAOYSA-N 0.000 description 1
- 229910017464 nitrogen compound Inorganic materials 0.000 description 1
- 150000002830 nitrogen compounds Chemical class 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- ABLZXFCXXLZCGV-UHFFFAOYSA-N phosphonic acid group Chemical class P(O)(O)=O ABLZXFCXXLZCGV-UHFFFAOYSA-N 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- GTZCVFVGUGFEME-UHFFFAOYSA-N trans-aconitic acid Natural products OC(=O)CC(C(O)=O)=CC(O)=O GTZCVFVGUGFEME-UHFFFAOYSA-N 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- ZTWTYVWXUKTLCP-UHFFFAOYSA-N vinylphosphonic acid Chemical compound OP(O)(=O)C=C ZTWTYVWXUKTLCP-UHFFFAOYSA-N 0.000 description 1
- NLVXSWCKKBEXTG-UHFFFAOYSA-N vinylsulfonic acid Chemical compound OS(=O)(=O)C=C NLVXSWCKKBEXTG-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Electrostatic Separation (AREA)
- Saccharide Compounds (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE7514049A SE422371B (sv) | 1975-12-12 | 1975-12-12 | Sett att generera en ph-funktion for anvendning vid elektrofores |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1570698A true GB1570698A (en) | 1980-07-09 |
Family
ID=20326321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB51757/76A Expired GB1570698A (en) | 1975-12-12 | 1976-12-10 | Method of generating a ph-gradient especially for use in electrophoresis |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US4130470A (enExample) |
| JP (1) | JPS5910492B2 (enExample) |
| DE (1) | DE2656162C3 (enExample) |
| FR (1) | FR2334951A1 (enExample) |
| GB (1) | GB1570698A (enExample) |
| SE (1) | SE422371B (enExample) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1979000002A1 (en) | 1977-06-15 | 1979-01-11 | Nat Res Dev | Improvements relating to membrane electrophoresis |
| US4490381A (en) * | 1979-11-13 | 1984-12-25 | Imperial Chemical Industries Plc | 1'-Substituted spiro[imidazolidine-4,3'-indoline]2,2',5-triones |
| US4542200A (en) * | 1982-02-19 | 1985-09-17 | Fmc Corporation | Polyacrylamide cross-linked with a polysaccharide resin as electrophoretic gel medium |
| US4504641A (en) * | 1982-02-19 | 1985-03-12 | Fmc Corporation | Polyacrylamide cross-linked with a polysaccharide resin as electrophoretic gel medium |
| JPS59171848A (ja) * | 1983-03-18 | 1984-09-28 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| JPS59171849A (ja) * | 1983-03-18 | 1984-09-28 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| JPS6060548A (ja) * | 1983-09-14 | 1985-04-08 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| SE8801081L (sv) * | 1988-03-24 | 1989-09-25 | Pharmacia Ab | Lagringsstabil akrylamidderivatkomposition foer generering av immobiliserade ph-gradienter |
| US4925545A (en) * | 1989-01-31 | 1990-05-15 | Amest, Inc. | Method of generating pH functions in electrophoresis and isoelectric focusing |
| US4963236A (en) * | 1989-03-08 | 1990-10-16 | Ampholife Technologies | Apparatus and methods for isoelectric focusing |
| US5160594A (en) * | 1989-03-08 | 1992-11-03 | Board Of Regents Of The University Of Texas System | Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers |
| US5773645A (en) * | 1997-05-05 | 1998-06-30 | Bio-Rad Laboratories, Inc. | Two-dimensional electrophoresis device |
| US5993627A (en) * | 1997-06-24 | 1999-11-30 | Large Scale Biology Corporation | Automated system for two-dimensional electrophoresis |
| US6554991B1 (en) | 1997-06-24 | 2003-04-29 | Large Scale Proteomics Corporation | Automated system for two-dimensional electrophoresis |
| RU2140073C1 (ru) * | 1998-01-30 | 1999-10-20 | Стоянов Александр Владимирович | Способ формирования сред с заданным профилем ph |
| US7517442B1 (en) | 1999-08-09 | 2009-04-14 | Life Technologies Corporation | Facile method and apparatus for the analysis of biological macromolecules in two dimensions using common and familiar electrophoresis formats |
| US7195923B2 (en) * | 2001-01-31 | 2007-03-27 | Scripps Laboratories, Inc. | Ratiometric determination of glycated protein |
| JP4236938B2 (ja) * | 2001-05-10 | 2009-03-11 | インビトロジェン コーポレイション | 予め注型された水和可能分離媒体を電気泳動するための方法および装置 |
| US7601251B2 (en) * | 2001-05-10 | 2009-10-13 | Life Technologies Corporation | Methods and apparatus for low resistance electrophoresis of prior-cast, hydratable separation media |
| US20060096906A1 (en) * | 2002-10-13 | 2006-05-11 | Adam Rubin | Microfluid biomolecule separation system |
| US7622028B2 (en) * | 2003-05-09 | 2009-11-24 | Life Technologies Corporation | Solution phase electrophoresis device, components, and methods |
| US7850835B2 (en) * | 2003-05-09 | 2010-12-14 | Life Technologies Corporation | Solution phase electrophoresis device, components, and methods |
| AU2004269196B2 (en) * | 2003-09-03 | 2010-03-04 | Shmuel Bukshpan | Methods and apparatus for rapid crystallization of biomolecules |
| EP1671112A4 (en) * | 2003-10-07 | 2011-03-23 | Life Technologies Corp | IMPROVED GELE FOR ISOELECTRIC FOCUSING AND METHOD FOR USE THEREOF |
| SE0302827D0 (sv) | 2003-10-23 | 2003-10-23 | Amersham Biosciences Ab | Method for synthesis of acrylamide derivatives |
| US7407816B2 (en) * | 2004-05-07 | 2008-08-05 | Gentius, Inc | Isoelectric particles and uses thereof |
| WO2007126354A1 (en) * | 2006-04-27 | 2007-11-08 | Ge Healthcare Bio-Sciences Ab | Electrodic bridge |
| US20110100821A1 (en) * | 2007-04-20 | 2011-05-05 | Brian Furmanski | Mineral oil free isoelectric focusing apparatus for immobilized ph gradient strips |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2731411A (en) * | 1951-12-05 | 1956-01-17 | Ionics | Electrically conductive membranes and the like comprising the sulfonated polymerizates of polyvinyl aryl compounds |
| US2976576A (en) * | 1956-04-24 | 1961-03-28 | Wichterle Otto | Process for producing shaped articles from three-dimensional hydrophilic high polymers |
| SE314227B (enExample) * | 1964-08-26 | 1969-09-01 | Lkb Produkter Ab | |
| US3523863A (en) * | 1966-11-09 | 1970-08-11 | Beckman Instruments Inc | Drying and preservation of electrophoresis gel films |
| GB1223589A (en) * | 1967-12-18 | 1971-02-24 | South African Inventions | Improvements relating to a medium for separations |
| US3704217A (en) * | 1969-09-08 | 1972-11-28 | Samuel T Nerenberg | Segmental macromolecular separation method and apparatus |
| SE357891B (enExample) * | 1970-04-08 | 1973-07-16 | Lkb Produkter Ab | |
| US3664939A (en) * | 1970-04-22 | 1972-05-23 | Univ California | ISOELECTRIC FOCUSING AND FRACTIONATION AMPHOLYTES IN THERMALLY ENGENDERED pH GRADIENTS |
| BE789188A (fr) * | 1971-09-24 | 1973-01-15 | Orion Yhtymae Oy | Procede pour la determination quantitative et qualitative de molecules ayant des proprietes antigenes |
| DE2234000B2 (de) * | 1972-07-11 | 1974-08-08 | Maurer, Sigrid, 7400 Tuebingen | Gel-Fertigpackung für elektrochemische Trennungen |
| GB1359944A (en) * | 1972-07-18 | 1974-07-17 | Miles Lab | Apparatus for gel electrophoresis |
| US3873514A (en) * | 1974-05-17 | 1975-03-25 | Bio Rad Laboratories | Preparation of gel for affinity chromatography |
| US3948743A (en) * | 1975-04-14 | 1976-04-06 | Bio-Rad Laboratories | Method for gel electrophoresis |
-
1975
- 1975-12-12 SE SE7514049A patent/SE422371B/xx not_active IP Right Cessation
-
1976
- 1976-12-07 US US05/748,184 patent/US4130470A/en not_active Expired - Lifetime
- 1976-12-10 GB GB51757/76A patent/GB1570698A/en not_active Expired
- 1976-12-10 JP JP51149299A patent/JPS5910492B2/ja not_active Expired
- 1976-12-10 DE DE2656162A patent/DE2656162C3/de not_active Expired
- 1976-12-10 FR FR7637402A patent/FR2334951A1/fr active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| DE2656162C3 (de) | 1982-02-25 |
| DE2656162B2 (de) | 1980-11-20 |
| SE422371B (sv) | 1982-03-01 |
| JPS5273088A (en) | 1977-06-18 |
| DE2656162A1 (de) | 1977-09-29 |
| FR2334951A1 (fr) | 1977-07-08 |
| FR2334951B1 (enExample) | 1980-04-30 |
| SE7514049L (sv) | 1977-06-13 |
| US4130470A (en) | 1978-12-19 |
| JPS5910492B2 (ja) | 1984-03-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| PE20 | Patent expired after termination of 20 years |
Effective date: 19961209 |