GB1388725A - Enzyme adsorbing matrices for use in the purification and storage of enzymes - Google Patents
Enzyme adsorbing matrices for use in the purification and storage of enzymesInfo
- Publication number
- GB1388725A GB1388725A GB2205772A GB2205772A GB1388725A GB 1388725 A GB1388725 A GB 1388725A GB 2205772 A GB2205772 A GB 2205772A GB 2205772 A GB2205772 A GB 2205772A GB 1388725 A GB1388725 A GB 1388725A
- Authority
- GB
- United Kingdom
- Prior art keywords
- cellulose
- trypsin
- enzyme
- enzymes
- crosslinked
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 9
- 102000004190 Enzymes Human genes 0.000 title abstract 9
- 238000000746 purification Methods 0.000 title 1
- 229920002678 cellulose Polymers 0.000 abstract 7
- 239000001913 cellulose Substances 0.000 abstract 7
- 229940088598 enzyme Drugs 0.000 abstract 7
- 239000011159 matrix material Substances 0.000 abstract 6
- -1 p-aminobenzyl Chemical group 0.000 abstract 5
- 239000003446 ligand Substances 0.000 abstract 4
- 239000004365 Protease Substances 0.000 abstract 3
- 108090000631 Trypsin Proteins 0.000 abstract 3
- 102000004142 Trypsin Human genes 0.000 abstract 3
- 239000012588 trypsin Substances 0.000 abstract 3
- 230000001810 trypsinlike Effects 0.000 abstract 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 2
- XKUKSGPZAADMRA-UHFFFAOYSA-N glycyl-glycyl-glycine Chemical compound NCC(=O)NCC(=O)NCC(O)=O XKUKSGPZAADMRA-UHFFFAOYSA-N 0.000 abstract 2
- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 abstract 2
- HXKWSIPCTVYMBN-UHFFFAOYSA-N 2-(4-aminophenyl)guanidine Chemical compound NC(=N)NC1=CC=C(N)C=C1 HXKWSIPCTVYMBN-UHFFFAOYSA-N 0.000 abstract 1
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 abstract 1
- 125000000022 2-aminoethyl group Chemical group [H]C([*])([H])C([H])([H])N([H])[H] 0.000 abstract 1
- VWDSNVUXQDDXBN-UHFFFAOYSA-N 3-aminobenzenecarboximidamide Chemical compound NC(=N)C1=CC=CC(N)=C1 VWDSNVUXQDDXBN-UHFFFAOYSA-N 0.000 abstract 1
- SLXKOJJOQWFEFD-UHFFFAOYSA-N 6-aminohexanoic acid Chemical compound NCCCCCC(O)=O SLXKOJJOQWFEFD-UHFFFAOYSA-N 0.000 abstract 1
- 229920001817 Agar Polymers 0.000 abstract 1
- 229920000936 Agarose Polymers 0.000 abstract 1
- 108010004032 Bromelains Proteins 0.000 abstract 1
- 102000003670 Carboxypeptidase B Human genes 0.000 abstract 1
- 108090000087 Carboxypeptidase B Proteins 0.000 abstract 1
- 102000008186 Collagen Human genes 0.000 abstract 1
- 108010035532 Collagen Proteins 0.000 abstract 1
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 abstract 1
- 229920002307 Dextran Polymers 0.000 abstract 1
- 108010088842 Fibrinolysin Proteins 0.000 abstract 1
- 108090000270 Ficain Proteins 0.000 abstract 1
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 abstract 1
- 108010008488 Glycylglycine Proteins 0.000 abstract 1
- 108090000526 Papain Proteins 0.000 abstract 1
- 108090000190 Thrombin Proteins 0.000 abstract 1
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 abstract 1
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 abstract 1
- 125000001931 aliphatic group Chemical group 0.000 abstract 1
- 229960002684 aminocaproic acid Drugs 0.000 abstract 1
- 125000003118 aryl group Chemical group 0.000 abstract 1
- LZCZIHQBSCVGRD-UHFFFAOYSA-N benzenecarboximidamide;hydron;chloride Chemical compound [Cl-].NC(=[NH2+])C1=CC=CC=C1 LZCZIHQBSCVGRD-UHFFFAOYSA-N 0.000 abstract 1
- UCMIRNVEIXFBKS-UHFFFAOYSA-N beta-alanine Chemical compound NCCC(O)=O UCMIRNVEIXFBKS-UHFFFAOYSA-N 0.000 abstract 1
- OTBHHUPVCYLGQO-UHFFFAOYSA-N bis(3-aminopropyl)amine Chemical compound NCCCNCCCN OTBHHUPVCYLGQO-UHFFFAOYSA-N 0.000 abstract 1
- 235000019835 bromelain Nutrition 0.000 abstract 1
- 229920001436 collagen Polymers 0.000 abstract 1
- 230000002860 competitive effect Effects 0.000 abstract 1
- 125000004956 cyclohexylene group Chemical group 0.000 abstract 1
- 235000019836 ficin Nutrition 0.000 abstract 1
- POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 abstract 1
- 108010067216 glycyl-glycyl-glycine Proteins 0.000 abstract 1
- 229940043257 glycylglycine Drugs 0.000 abstract 1
- NAQMVNRVTILPCV-UHFFFAOYSA-N hexane-1,6-diamine Chemical compound NCCCCCCN NAQMVNRVTILPCV-UHFFFAOYSA-N 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- 125000000843 phenylene group Chemical group C1(=C(C=CC=C1)*)* 0.000 abstract 1
- 229940080469 phosphocellulose Drugs 0.000 abstract 1
- 229940012957 plasmin Drugs 0.000 abstract 1
- 229920002401 polyacrylamide Polymers 0.000 abstract 1
- 238000001179 sorption measurement Methods 0.000 abstract 1
- 229960004072 thrombin Drugs 0.000 abstract 1
- 229960005356 urokinase Drugs 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B15/00—Preparation of other cellulose derivatives or modified cellulose, e.g. complexes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Polymers & Plastics (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14277871A | 1971-05-12 | 1971-05-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1388725A true GB1388725A (en) | 1975-03-26 |
Family
ID=22501228
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB2205772A Expired GB1388725A (en) | 1971-05-12 | 1972-05-11 | Enzyme adsorbing matrices for use in the purification and storage of enzymes |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US3746622A (https=) |
| JP (3) | JPS5713266B1 (https=) |
| BE (1) | BE783439A (https=) |
| CA (1) | CA986046A (https=) |
| CH (1) | CH590297A5 (https=) |
| DE (1) | DE2220568C2 (https=) |
| FR (1) | FR2137610B1 (https=) |
| GB (1) | GB1388725A (https=) |
| IT (1) | IT965051B (https=) |
| SE (1) | SE406913B (https=) |
| ZA (1) | ZA723163B (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3983001A (en) * | 1972-05-10 | 1976-09-28 | Ceskoslovenska Akademie Ved | Isolation of biologically active compounds by affinity chromatography |
| DE2247163A1 (de) * | 1972-09-26 | 1974-03-28 | Merck Patent Gmbh | Traegermatrix zur fixierung biologisch wirksamer stoffe und verfahren zu ihrer herstellung |
| FR2234311B1 (https=) * | 1973-06-21 | 1976-04-30 | Air Liquide | |
| US4010074A (en) * | 1974-01-22 | 1977-03-01 | The Green Cross Corporation | Method for purification and recovery of urokinase |
| FR2268022B1 (https=) * | 1974-04-22 | 1977-06-24 | Rhone Poulenc Ind | |
| FR2366306A1 (fr) * | 1976-02-20 | 1978-04-28 | Anvar | Nouveau procede de preparation de clostripaine pure et clostripaine pure lyophilisee obtenue par ce procede |
| US4225675A (en) * | 1977-01-14 | 1980-09-30 | Nippon Soda Company, Ltd. | Adsorbent for urokinase and process for the production of urokinase |
| NL7800200A (nl) * | 1977-01-14 | 1978-07-18 | Nippon Soda Co | Adsorptiemiddel voor urokinase en werkwijze voor de bereiding van urokinase. |
| US4259448A (en) * | 1978-01-03 | 1981-03-31 | Nippon Soda Company, Ltd. | Protein adsorbent and process for the purification of urokinase |
| JPS5517364A (en) * | 1978-07-25 | 1980-02-06 | Toho Yakuhin Kogyo Kk | Purification of pancreatic kallikrein |
| US4406826A (en) * | 1981-03-05 | 1983-09-27 | W. R. Grace & Co. | Heat curable conductive ink |
| US4443495A (en) * | 1981-03-05 | 1984-04-17 | W. R. Grace & Co. | Heat curable conductive ink |
| DE3249491T1 (de) * | 1982-05-31 | 1984-04-19 | Mochida Pharmaceutical Co., Ltd., Tokyo | Immobilisiertes Pepsin zur Verwendung beim Entfernen von Immunkomplex aus dem Blut und eine Verfahren zur Entfernung von Immunkomplex aus dem Blut unter Verwendung des immobilisierten Pepsins |
| CN101693748B (zh) * | 2009-10-15 | 2011-12-07 | 南昌市浩然生物医药有限公司 | 一种特异性纯化高分子尿激酶层析介质及制备方法和应用 |
| US8377672B2 (en) | 2010-02-18 | 2013-02-19 | 3M Innovative Properties Company | Ligand functionalized polymers |
| KR102793894B1 (ko) | 2019-06-28 | 2025-04-08 | 삼성전자주식회사 | 디지털 픽셀 및 이를 포함하는 이미지 센서 |
-
1971
- 1971-05-12 US US00142778A patent/US3746622A/en not_active Expired - Lifetime
-
1972
- 1972-03-28 CA CA138,314A patent/CA986046A/en not_active Expired
- 1972-04-25 JP JP4170372A patent/JPS5713266B1/ja active Pending
- 1972-04-26 DE DE2220568A patent/DE2220568C2/de not_active Expired
- 1972-05-08 FR FR7216331A patent/FR2137610B1/fr not_active Expired
- 1972-05-08 SE SE7206026A patent/SE406913B/sv unknown
- 1972-05-09 IT IT24125/72A patent/IT965051B/it active
- 1972-05-09 ZA ZA723163A patent/ZA723163B/xx unknown
- 1972-05-10 CH CH698672A patent/CH590297A5/xx not_active IP Right Cessation
- 1972-05-11 GB GB2205772A patent/GB1388725A/en not_active Expired
- 1972-05-12 BE BE783439A patent/BE783439A/xx not_active IP Right Cessation
-
1979
- 1979-03-02 JP JP2438079A patent/JPS54157893A/ja active Granted
-
1982
- 1982-04-19 JP JP57065236A patent/JPS5839513B2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DE2220568C2 (de) | 1987-01-02 |
| IT965051B (it) | 1974-01-31 |
| JPS5839513B2 (ja) | 1983-08-30 |
| CH590297A5 (https=) | 1977-07-29 |
| CA986046A (en) | 1976-03-23 |
| JPS54157893A (en) | 1979-12-13 |
| FR2137610A1 (https=) | 1972-12-29 |
| US3746622A (en) | 1973-07-17 |
| JPS57198086A (en) | 1982-12-04 |
| BE783439A (fr) | 1972-11-13 |
| ZA723163B (en) | 1973-05-30 |
| JPS5759755B2 (https=) | 1982-12-16 |
| SE406913B (sv) | 1979-03-05 |
| FR2137610B1 (https=) | 1976-10-29 |
| JPS5713266B1 (https=) | 1982-03-16 |
| DE2220568A1 (de) | 1972-11-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed [section 19, patents act 1949] | ||
| 746 | Register noted 'licences of right' (sect. 46/1977) | ||
| PCNP | Patent ceased through non-payment of renewal fee |