GB1223763A - Process for the preparation of chymotrypsin b - Google Patents

Process for the preparation of chymotrypsin b

Info

Publication number
GB1223763A
GB1223763A GB5116068A GB5116068A GB1223763A GB 1223763 A GB1223763 A GB 1223763A GB 5116068 A GB5116068 A GB 5116068A GB 5116068 A GB5116068 A GB 5116068A GB 1223763 A GB1223763 A GB 1223763A
Authority
GB
United Kingdom
Prior art keywords
chymotrypsin
ammonium sulphate
proteins
cake
added
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB5116068A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OPOCHIMIE L
Original Assignee
OPOCHIMIE L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by OPOCHIMIE L filed Critical OPOCHIMIE L
Publication of GB1223763A publication Critical patent/GB1223763A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

1,223,763. Chymotrypsin B. L'OPOCHIMIE. 29 Oct., 1968 [3 Nov., 1967], No. 51160/68. Heading C3H. In a process for obtaining chymotrypsin B by a fractionated precipitation of the proteins contained in a pulpy pancreas mixture followed by dissolution of the final cake in cold water, activation with trypsin and chromatographic purification, a first amount of ammonium sulphate is added to reach a degree of saturation of about 0À2, the mixture obtained is then filtered free of proteins of very high molecular weight and non-proteinaceous impurities, a second amount of ammonium sulphate is added to the filtrate to reach a degree of saturation comprised between 0À4 and 0À5 and the resulting mixture is filtered, giving a final cake the weight of which is approximately equal to 20% of that of the raw mixture of precipitable proteins contained in the aqueous extract before adding thereto said second amount of ammonium sulphate, said cake is redissolved in water, trypsin is added to the resulting solution in a quantity substantially equal to 1/40 of the weight of proteins contained in said solution, as determined from spectrophotometric readings, and the chymotrypsin B contained in the trypsinactivated solution is separated from other enzymes and proteins by a single chromatography. The cake may be dialysed to remove ammonium sulphate before the activation step.
GB5116068A 1967-11-03 1968-10-29 Process for the preparation of chymotrypsin b Expired GB1223763A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR126852 1967-11-03

Publications (1)

Publication Number Publication Date
GB1223763A true GB1223763A (en) 1971-03-03

Family

ID=8641174

Family Applications (1)

Application Number Title Priority Date Filing Date
GB5116068A Expired GB1223763A (en) 1967-11-03 1968-10-29 Process for the preparation of chymotrypsin b

Country Status (8)

Country Link
BE (1) BE722896A (en)
CH (1) CH491967A (en)
DE (1) DE1806625A1 (en)
DK (1) DK122818B (en)
FR (1) FR1552038A (en)
GB (1) GB1223763A (en)
IE (1) IE32447B1 (en)
NL (1) NL6815600A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105400765A (en) * 2015-11-21 2016-03-16 青岛康原药业有限公司 Method for conducting affinity chromatography, stepwise elution and purification on chymotrypsin and medicine composition for improving re-dissolution of chymotrypsin

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110964707A (en) * 2019-12-31 2020-04-07 江西浩然生物制药有限公司 Extraction and purification method of chymotrypsin

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105400765A (en) * 2015-11-21 2016-03-16 青岛康原药业有限公司 Method for conducting affinity chromatography, stepwise elution and purification on chymotrypsin and medicine composition for improving re-dissolution of chymotrypsin

Also Published As

Publication number Publication date
DK122818B (en) 1972-04-17
IE32447B1 (en) 1973-08-08
NL6815600A (en) 1969-05-06
IE32447L (en) 1969-05-03
FR1552038A (en) 1969-01-03
DE1806625A1 (en) 1969-12-11
BE722896A (en) 1969-04-25
CH491967A (en) 1970-06-15

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