IE32447B1 - Process for the preparation of chymotrypsin b - Google Patents
Process for the preparation of chymotrypsin bInfo
- Publication number
- IE32447B1 IE32447B1 IE128868A IE128868A IE32447B1 IE 32447 B1 IE32447 B1 IE 32447B1 IE 128868 A IE128868 A IE 128868A IE 128868 A IE128868 A IE 128868A IE 32447 B1 IE32447 B1 IE 32447B1
- Authority
- IE
- Ireland
- Prior art keywords
- chymotrypsin
- ammonium sulphate
- proteins
- cake
- added
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
1,223,763. Chymotrypsin B. L'OPOCHIMIE. 29 Oct., 1968 [3 Nov., 1967], No. 51160/68. Heading C3H. In a process for obtaining chymotrypsin B by a fractionated precipitation of the proteins contained in a pulpy pancreas mixture followed by dissolution of the final cake in cold water, activation with trypsin and chromatographic purification, a first amount of ammonium sulphate is added to reach a degree of saturation of about 0À2, the mixture obtained is then filtered free of proteins of very high molecular weight and non-proteinaceous impurities, a second amount of ammonium sulphate is added to the filtrate to reach a degree of saturation comprised between 0À4 and 0À5 and the resulting mixture is filtered, giving a final cake the weight of which is approximately equal to 20% of that of the raw mixture of precipitable proteins contained in the aqueous extract before adding thereto said second amount of ammonium sulphate, said cake is redissolved in water, trypsin is added to the resulting solution in a quantity substantially equal to 1/40 of the weight of proteins contained in said solution, as determined from spectrophotometric readings, and the chymotrypsin B contained in the trypsinactivated solution is separated from other enzymes and proteins by a single chromatography. The cake may be dialysed to remove ammonium sulphate before the activation step.
[GB1223763A]
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR126852 | 1967-11-03 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| IE32447L IE32447L (en) | 1969-05-03 |
| IE32447B1 true IE32447B1 (en) | 1973-08-08 |
Family
ID=8641174
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IE128868A IE32447B1 (en) | 1967-11-03 | 1968-10-25 | Process for the preparation of chymotrypsin b |
Country Status (8)
| Country | Link |
|---|---|
| BE (1) | BE722896A (en) |
| CH (1) | CH491967A (en) |
| DE (1) | DE1806625A1 (en) |
| DK (1) | DK122818B (en) |
| FR (1) | FR1552038A (en) |
| GB (1) | GB1223763A (en) |
| IE (1) | IE32447B1 (en) |
| NL (1) | NL6815600A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105400765A (en) * | 2015-11-21 | 2016-03-16 | 青岛康原药业有限公司 | Method for conducting affinity chromatography, stepwise elution and purification on chymotrypsin and medicine composition for improving re-dissolution of chymotrypsin |
| CN110964707A (en) * | 2019-12-31 | 2020-04-07 | 江西浩然生物制药有限公司 | Extraction and purification method of chymotrypsin |
-
1967
- 1967-11-03 FR FR1552038D patent/FR1552038A/fr not_active Expired
-
1968
- 1968-10-25 IE IE128868A patent/IE32447B1/en unknown
- 1968-10-25 BE BE722896D patent/BE722896A/xx unknown
- 1968-10-29 GB GB5116068A patent/GB1223763A/en not_active Expired
- 1968-10-31 CH CH1620868A patent/CH491967A/en not_active IP Right Cessation
- 1968-11-01 DK DK532068A patent/DK122818B/en unknown
- 1968-11-01 NL NL6815600A patent/NL6815600A/xx unknown
- 1968-11-02 DE DE19681806625 patent/DE1806625A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| NL6815600A (en) | 1969-05-06 |
| CH491967A (en) | 1970-06-15 |
| FR1552038A (en) | 1969-01-03 |
| DK122818B (en) | 1972-04-17 |
| GB1223763A (en) | 1971-03-03 |
| IE32447L (en) | 1969-05-03 |
| DE1806625A1 (en) | 1969-12-11 |
| BE722896A (en) | 1969-04-25 |
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