FR2047889A1 - Fibrinolytic enzyme separation from mould - fungus cultures - Google Patents
Fibrinolytic enzyme separation from mould - fungus culturesInfo
- Publication number
- FR2047889A1 FR2047889A1 FR7000610A FR7000610A FR2047889A1 FR 2047889 A1 FR2047889 A1 FR 2047889A1 FR 7000610 A FR7000610 A FR 7000610A FR 7000610 A FR7000610 A FR 7000610A FR 2047889 A1 FR2047889 A1 FR 2047889A1
- Authority
- FR
- France
- Prior art keywords
- sephadex
- dissolving
- active ingredient
- fibrinolytic enzyme
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
- B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
- B29C66/00—General aspects of processes or apparatus for joining preformed parts
- B29C66/01—General aspects dealing with the joint area or with the area to be joined
- B29C66/05—Particular design of joint configurations
- B29C66/10—Particular design of joint configurations particular design of the joint cross-sections
- B29C66/11—Joint cross-sections comprising a single joint-segment, i.e. one of the parts to be joined comprising a single joint-segment in the joint cross-section
- B29C66/112—Single lapped joints
- B29C66/1122—Single lap to lap joints, i.e. overlap joints
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
Highly purified fibrinolytic enzyme for dissolving arterial and venous thrombi is prepared by extracting crude enzyme mixture from Aspergillus ochraceus with aqs. buffer solution (pref. with 0.05-0.1 M tris-HCl buffer or 0.05-0.1 M NH4 acetate buffer of pH 6.5-7.5, partic. 7-7.2); filtering the extract containing active ingredient over an anion exchanger DEAE-Sephadex or cellulose; precipitating the active ingredient from the filtrate with water-miscible organic solvents; re-dissolving the precipitate with water after drying in vacuo and subjecting it to gel chromatography on cross-linked dextran gel Sephadex G-75 or Sephadex G-100 and freeze drying the protease-containing fractions.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DD14036369 | 1969-06-09 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2047889A1 true FR2047889A1 (en) | 1971-03-19 |
| FR2047889B1 FR2047889B1 (en) | 1974-04-12 |
Family
ID=5481312
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7000610A Granted FR2047889A1 (en) | 1969-06-09 | 1970-01-08 | Fibrinolytic enzyme separation from mould - fungus cultures |
Country Status (9)
| Country | Link |
|---|---|
| AT (1) | AT296195B (en) |
| CS (1) | CS151021B2 (en) |
| DE (1) | DE2024251C3 (en) |
| DK (1) | DK130005B (en) |
| FR (1) | FR2047889A1 (en) |
| HU (1) | HU163467B (en) |
| RO (1) | RO57948A (en) |
| SE (1) | SE378602B (en) |
| SU (1) | SU375829A3 (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2468081C2 (en) * | 2011-03-09 | 2012-11-27 | Александр Андреевич Осмоловский | Method of obtaining proteinase-activator of protein c in blood plasma |
| RU2448158C1 (en) * | 2011-03-24 | 2012-04-20 | Эспосито Трейдинг Лтд | Method for preparing preparation fortelysin showing fibrinolytic properties, and its application for treating myocardial infraction |
-
1970
- 1970-01-08 FR FR7000610A patent/FR2047889A1/en active Granted
- 1970-05-19 DE DE2024251A patent/DE2024251C3/en not_active Expired
- 1970-05-20 AT AT04523/70A patent/AT296195B/en not_active IP Right Cessation
- 1970-06-02 CS CS3851A patent/CS151021B2/cs unknown
- 1970-06-04 DK DK289370AA patent/DK130005B/en unknown
- 1970-06-08 SU SU1447197A patent/SU375829A3/ru active
- 1970-06-08 HU HUAE320A patent/HU163467B/hu unknown
- 1970-06-09 SE SE7007982A patent/SE378602B/xx unknown
- 1970-06-09 RO RO63586A patent/RO57948A/ro unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE2024251A1 (en) | 1971-02-11 |
| AT296195B (en) | 1972-01-15 |
| CS151021B2 (en) | 1973-09-17 |
| DE2024251C3 (en) | 1980-04-03 |
| RO57948A (en) | 1975-06-15 |
| DK130005C (en) | 1975-06-09 |
| SE378602B (en) | 1975-09-08 |
| DK130005B (en) | 1974-12-09 |
| SU375829A3 (en) | 1973-03-23 |
| FR2047889B1 (en) | 1974-04-12 |
| DE2024251B2 (en) | 1979-08-02 |
| HU163467B (en) | 1973-09-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |