GB1230279A - - Google Patents

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Publication number
GB1230279A
GB1230279A GB1230279DA GB1230279A GB 1230279 A GB1230279 A GB 1230279A GB 1230279D A GB1230279D A GB 1230279DA GB 1230279 A GB1230279 A GB 1230279A
Authority
GB
United Kingdom
Prior art keywords
cross
water
linked dextran
dextran gel
diethylaminoethyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of GB1230279A publication Critical patent/GB1230279A/en
Expired legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00Medicinal preparations containing peptides

Abstract

1,230,279. Fibrinolytic enzyme. ARZNEIMITTELWERK DRESDEN VEB. 11 Dec., 1969, No. 60541/69. Heading C3H. A process for the isolation of a fibrinolytic enzyme from mould fungus cultures, wherein a crude enzyme mixture from Aspergillus ochraceus is extracted with an aqueous buffer solution, the extract containing the active material is filtered through a diethylaminoethyl-groupcontaining cross-linked dextran gel anion exchanger or through a diethylaminoethyl-cellulose anion exchanger, the active material precipitated from the filtrate obtained by means of a water-miscible organic solvent, the precipitate obtained separated and dried in a vacuum, again dissolved in water and subjected to gel chromatography on cross-linked dextran gel and the protease-containing fractions obtained then lyophilised. The cross-linked dextran gel must be one capable of fractionating substrates of M.Wt. 1000 to 100,000 and have a water regain of 7À5-10; suitable commercially available types are mentioned.
GB1230279D 1969-12-11 1969-12-11 Expired GB1230279A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB6054169 1969-12-11

Publications (1)

Publication Number Publication Date
GB1230279A true GB1230279A (en) 1971-04-28

Family

ID=10485725

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1230279D Expired GB1230279A (en) 1969-12-11 1969-12-11

Country Status (1)

Country Link
GB (1) GB1230279A (en)

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee