GB1230279A - - Google Patents
Info
- Publication number
- GB1230279A GB1230279A GB1230279DA GB1230279A GB 1230279 A GB1230279 A GB 1230279A GB 1230279D A GB1230279D A GB 1230279DA GB 1230279 A GB1230279 A GB 1230279A
- Authority
- GB
- United Kingdom
- Prior art keywords
- cross
- water
- linked dextran
- dextran gel
- diethylaminoethyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Abstract
1,230,279. Fibrinolytic enzyme. ARZNEIMITTELWERK DRESDEN VEB. 11 Dec., 1969, No. 60541/69. Heading C3H. A process for the isolation of a fibrinolytic enzyme from mould fungus cultures, wherein a crude enzyme mixture from Aspergillus ochraceus is extracted with an aqueous buffer solution, the extract containing the active material is filtered through a diethylaminoethyl-groupcontaining cross-linked dextran gel anion exchanger or through a diethylaminoethyl-cellulose anion exchanger, the active material precipitated from the filtrate obtained by means of a water-miscible organic solvent, the precipitate obtained separated and dried in a vacuum, again dissolved in water and subjected to gel chromatography on cross-linked dextran gel and the protease-containing fractions obtained then lyophilised. The cross-linked dextran gel must be one capable of fractionating substrates of M.Wt. 1000 to 100,000 and have a water regain of 7À5-10; suitable commercially available types are mentioned.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB6054169 | 1969-12-11 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1230279A true GB1230279A (en) | 1971-04-28 |
Family
ID=10485725
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB1230279D Expired GB1230279A (en) | 1969-12-11 | 1969-12-11 |
Country Status (1)
Country | Link |
---|---|
GB (1) | GB1230279A (en) |
-
1969
- 1969-12-11 GB GB1230279D patent/GB1230279A/en not_active Expired
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |