FR3062395B1 - Microorganisme genetiquement optimise pour la production de molecules d'interet - Google Patents

Microorganisme genetiquement optimise pour la production de molecules d'interet Download PDF

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Publication number
FR3062395B1
FR3062395B1 FR1750696A FR1750696A FR3062395B1 FR 3062395 B1 FR3062395 B1 FR 3062395B1 FR 1750696 A FR1750696 A FR 1750696A FR 1750696 A FR1750696 A FR 1750696A FR 3062395 B1 FR3062395 B1 FR 3062395B1
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FR
France
Prior art keywords
interest
molecules
production
microorganism
genetically modified
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Expired - Fee Related
Application number
FR1750696A
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English (en)
French (fr)
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FR3062395A1 (fr
Inventor
Cedric Boisart
Nicolas Morin
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Enobraq
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Enobraq
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Priority to FR1750696A priority Critical patent/FR3062395B1/fr
Application filed by Enobraq filed Critical Enobraq
Priority to CN201880019539.8A priority patent/CN110475851A/zh
Priority to EP18702646.3A priority patent/EP3574082A1/fr
Priority to JP2019561348A priority patent/JP2020506722A/ja
Priority to BR112019015411-6A priority patent/BR112019015411A2/pt
Priority to KR1020197022622A priority patent/KR20190114982A/ko
Priority to PCT/EP2018/052004 priority patent/WO2018138288A1/fr
Priority to US16/480,579 priority patent/US20190359988A1/en
Publication of FR3062395A1 publication Critical patent/FR3062395A1/fr
Application granted granted Critical
Publication of FR3062395B1 publication Critical patent/FR3062395B1/fr
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C12P7/625—Polyesters of hydroxy carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01001—Transketolase (2.2.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01002—Transaldolase (2.2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01019—Phosphoribulokinase (2.7.1.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
FR1750696A 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet Expired - Fee Related FR3062395B1 (fr)

Priority Applications (8)

Application Number Priority Date Filing Date Title
FR1750696A FR3062395B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet
EP18702646.3A EP3574082A1 (fr) 2017-01-27 2018-01-26 Microorganisme génétiquement optimisé pour la production de molécules d'intérêt
JP2019561348A JP2020506722A (ja) 2017-01-27 2018-01-26 目的分子を産生するための遺伝的に最適化された微生物
BR112019015411-6A BR112019015411A2 (pt) 2017-01-27 2018-01-26 Microorganismo geneticamente otimizado para produzir moléculas de interesse
CN201880019539.8A CN110475851A (zh) 2017-01-27 2018-01-26 用于产生感兴趣的分子的经基因优化的微生物
KR1020197022622A KR20190114982A (ko) 2017-01-27 2018-01-26 대상 분자의 생산을 위한 유전자 최적화 미생물
PCT/EP2018/052004 WO2018138288A1 (fr) 2017-01-27 2018-01-26 Microorganisme génétiquement optimisé pour la production de molécules d'intérêt
US16/480,579 US20190359988A1 (en) 2017-01-27 2018-01-26 Genetically optimised microorganism for producing molecules of interest

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR1750696A FR3062395B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet
FR1750696 2017-01-27

Publications (2)

Publication Number Publication Date
FR3062395A1 FR3062395A1 (fr) 2018-08-03
FR3062395B1 true FR3062395B1 (fr) 2021-04-16

Family

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FR1750696A Expired - Fee Related FR3062395B1 (fr) 2017-01-27 2017-01-27 Microorganisme genetiquement optimise pour la production de molecules d'interet

Country Status (8)

Country Link
US (1) US20190359988A1 (enExample)
EP (1) EP3574082A1 (enExample)
JP (1) JP2020506722A (enExample)
KR (1) KR20190114982A (enExample)
CN (1) CN110475851A (enExample)
BR (1) BR112019015411A2 (enExample)
FR (1) FR3062395B1 (enExample)
WO (1) WO2018138288A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2958986B1 (en) * 2013-02-22 2022-09-07 DSM IP Assets B.V. Recombinant micro-organism for use in method with increased product yield
US11767521B2 (en) * 2020-02-21 2023-09-26 The Regents Of The University Of California Genetically modified bacterial cells and methods useful for producing indigoidine
CN118956840A (zh) * 2021-04-19 2024-11-15 武汉合生科技有限公司 获得α-法尼烯以及β-法尼烯的重组微生物及其构建方法
CN115259391B (zh) * 2022-08-26 2023-10-10 辽宁中森生物科技有限公司 一种污水处理用多元菌藻组合物、复合剂及其应用
CN117210446A (zh) * 2023-03-21 2023-12-12 北京蓝晶微生物科技有限公司 一种重建卡尔文循环提高异养微生物代谢产物产量的方法
CN117721054B (zh) * 2023-12-12 2025-01-28 四川大学 一种提高乳酸菌在盐胁迫下生物膜产量的方法
CN119709580A (zh) * 2025-03-04 2025-03-28 诸城市浩天药业有限公司 一种高效生产d-泛酸的工程菌株及其构建方法与应用
CN121450560B (zh) * 2026-01-06 2026-04-28 济南大学 一种生产对香豆酸的罗氏真养产碱工程菌株及其构建方法和应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RS54444B1 (sr) * 2011-07-12 2016-06-30 Scientist Of Fortune S.A. Rekombinantni mikroorganizmi za proizvodnju korisnih metabolita
FR3016371B1 (fr) * 2014-01-16 2018-02-02 Institut National De La Recherche Agronomique Levures modifiees pour utiliser le dioxyde de carbone

Also Published As

Publication number Publication date
KR20190114982A (ko) 2019-10-10
EP3574082A1 (fr) 2019-12-04
JP2020506722A (ja) 2020-03-05
BR112019015411A2 (pt) 2020-05-26
FR3062395A1 (fr) 2018-08-03
CN110475851A (zh) 2019-11-19
WO2018138288A1 (fr) 2018-08-02
US20190359988A1 (en) 2019-11-28

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